WO2001087914A3 - Plant gene targeting using oligonucleotides - Google Patents

Plant gene targeting using oligonucleotides Download PDF

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Publication number
WO2001087914A3
WO2001087914A3 PCT/US2001/016152 US0116152W WO0187914A3 WO 2001087914 A3 WO2001087914 A3 WO 2001087914A3 US 0116152 W US0116152 W US 0116152W WO 0187914 A3 WO0187914 A3 WO 0187914A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
gene targeting
plant gene
presented
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/016152
Other languages
French (fr)
Other versions
WO2001087914A2 (en
Inventor
Gregory D May
Eric B Kmiec
Michael C Rice
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Delaware
Original Assignee
University of Delaware
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Delaware filed Critical University of Delaware
Priority to EP01937552A priority Critical patent/EP1284985A2/en
Priority to AU2001263271A priority patent/AU2001263271A1/en
Priority to CA002409172A priority patent/CA2409172A1/en
Publication of WO2001087914A2 publication Critical patent/WO2001087914A2/en
Publication of WO2001087914A3 publication Critical patent/WO2001087914A3/en
Priority to US10/294,172 priority patent/US20030163849A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and compositions are presented for the generation of targeted alterations in a plant genome using double-stranded homogeneous oligonucleotides containing a single type of nucleotide. These methods can be used to correct mutations, introduce mutations and/or alter gene activity in a plant cell. A cell-free assay system for monitoring genetic alteration using the oligonucleotides of the invention is also presented.
PCT/US2001/016152 2000-05-17 2001-05-17 Plant gene targeting using oligonucleotides Ceased WO2001087914A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01937552A EP1284985A2 (en) 2000-05-17 2001-05-17 Plant gene targeting using oligonucleotides
AU2001263271A AU2001263271A1 (en) 2000-05-17 2001-05-17 Plant gene targeting using oligonucleotides
CA002409172A CA2409172A1 (en) 2000-05-17 2001-05-17 Plant gene targeting using oligonucleotides
US10/294,172 US20030163849A1 (en) 2000-05-17 2002-11-13 Plant gene targeting using oligonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20559700P 2000-05-17 2000-05-17
US60/205,597 2000-05-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/294,172 Continuation US20030163849A1 (en) 2000-05-17 2002-11-13 Plant gene targeting using oligonucleotides

Publications (2)

Publication Number Publication Date
WO2001087914A2 WO2001087914A2 (en) 2001-11-22
WO2001087914A3 true WO2001087914A3 (en) 2002-06-13

Family

ID=22762846

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/016152 Ceased WO2001087914A2 (en) 2000-05-17 2001-05-17 Plant gene targeting using oligonucleotides

Country Status (5)

Country Link
US (1) US20030163849A1 (en)
EP (1) EP1284985A2 (en)
AU (1) AU2001263271A1 (en)
CA (1) CA2409172A1 (en)
WO (1) WO2001087914A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006105436A2 (en) * 2005-03-30 2006-10-05 The Regents Of The University Of California Cloning and characterization of micrornas from rice
WO2007073149A1 (en) 2005-12-22 2007-06-28 Keygene N.V. Alternative nucleotides for improved targeted nucleotide exchange
ES2450743T3 (en) * 2007-12-21 2014-03-25 Keygene N.V. An improved mutagenesis method using polyethylene glycol by introduction of mutagenic nitrogen bases in plant protoplasts
MX2011002617A (en) 2008-09-11 2011-04-11 Keygene Nv Method for diagnostic marker development.
EP2376637A1 (en) 2008-12-22 2011-10-19 Keygene N.V. Use of double stranded rna to increase the efficiency of targeted gene alteration in plant protoplasts
EP2646549B1 (en) 2010-12-02 2015-02-18 Keygene N.V. Targeted alteration of dna
CN103502449B (en) 2010-12-02 2016-08-10 关键基因股份有限公司 Targeted changes to DNA with oligonucleotides
WO2016105185A1 (en) 2014-12-22 2016-06-30 Keygene N.V. Plant callus populations

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2326163A (en) * 1997-05-28 1998-12-16 Zeneca Ltd In situ modification of plant genes
WO1999025853A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
US6010907A (en) * 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
WO2001014531A1 (en) * 1999-08-20 2001-03-01 University Of Delaware Cell-free assay for plant gene targeting and conversion
WO2001073002A2 (en) * 2000-03-27 2001-10-04 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4459355A (en) * 1982-07-12 1984-07-10 International Paper Company Method for transforming plant cells
CA1313830C (en) * 1985-08-07 1993-02-23 Dilip Maganlal Shah Glyphosate-resistant plants
US5422251A (en) * 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
US5312910A (en) * 1987-05-26 1994-05-17 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5955363A (en) * 1990-01-03 1999-09-21 Promega Corporation Vector for in vitro mutagenesis and use thereof
US6136601A (en) * 1991-08-21 2000-10-24 Epoch Pharmaceuticals, Inc. Targeted mutagenesis in living cells using modified oligonucleotides
US5801154A (en) * 1993-10-18 1998-09-01 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of multidrug resistance-associated protein
DK0733059T3 (en) * 1993-12-09 2000-10-16 Univ Jefferson Compounds and Methods for Site-directed Mutations in Eukaryotic Cells
GB9408717D0 (en) * 1994-05-03 1994-06-22 Biotech & Biolog Scien Res DNA sequences
US5912340A (en) * 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
US5905185A (en) * 1995-11-30 1999-05-18 Ppl Therapeutics Protein C production in non-human transgenic mammals
US5731181A (en) * 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
US6004804A (en) * 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US6271360B1 (en) * 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2326163A (en) * 1997-05-28 1998-12-16 Zeneca Ltd In situ modification of plant genes
WO1999025853A1 (en) * 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
US6010907A (en) * 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
WO2001014531A1 (en) * 1999-08-20 2001-03-01 University Of Delaware Cell-free assay for plant gene targeting and conversion
WO2001073002A2 (en) * 2000-03-27 2001-10-04 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CULVER KENNETH W ET AL: "Correction of chromosomal point mutations in human cells with bifunctional oligonucleotides", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 10, October 1999 (1999-10-01), pages 989 - 993, XP002151266, ISSN: 1087-0156 *
GAMPER HOWARD B ET AL: "The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts.", NUCLEIC ACIDS RESEARCH, vol. 28, no. 21, 1 November 2000 (2000-11-01), pages 4332 - 4339, XP002192476, ISSN: 0305-1048 *
GAMPER HOWARD B JR ET AL: "A plausible mechanism for gene correction by chimeric oligonucleotides.", BIOCHEMISTRY, vol. 39, no. 19, 16 May 2000 (2000-05-16), pages 5808 - 5816, XP002192477, ISSN: 0006-2960 *
HUNGER-BERTLING K ET AL: "SHORT DNA FRAGMENTS INDUCE SITE SPECIFIC RECOMBINATION IN MAMMALIAN CELLS", MOLECULAR AND CELLULAR BIOCHEMISTRY, NORWELL, MA, US, vol. 92, 1990, pages 107 - 116, XP000972193, ISSN: 0300-8177 *
RICE M C ET AL: "GENETIC REPAIR OF MUTATIONS IN PLANT CELL-FREE EXTRACTS DIRECTED BYSPECIFIC CHIMERIC OLIGONUCLEOTIDES", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 123, no. 2, June 2000 (2000-06-01), pages 427 - 437, XP000945128, ISSN: 0032-0889 *

Also Published As

Publication number Publication date
WO2001087914A2 (en) 2001-11-22
EP1284985A2 (en) 2003-02-26
CA2409172A1 (en) 2001-11-22
US20030163849A1 (en) 2003-08-28
AU2001263271A1 (en) 2001-11-26

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