WO2001092566A3 - Immobilisierte nukleinsäuren und verwendungen davon - Google Patents

Immobilisierte nukleinsäuren und verwendungen davon Download PDF

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Publication number
WO2001092566A3
WO2001092566A3 PCT/EP2001/006014 EP0106014W WO0192566A3 WO 2001092566 A3 WO2001092566 A3 WO 2001092566A3 EP 0106014 W EP0106014 W EP 0106014W WO 0192566 A3 WO0192566 A3 WO 0192566A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
immobilized nucleic
nucleic acids
matrix
immobilized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/006014
Other languages
English (en)
French (fr)
Other versions
WO2001092566A2 (de
Inventor
Jens Burmeister
Petra Burgstaller
Sven Klussmann
Thomas Klein
Christian Frauendorf
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TME Pharma AG
Original Assignee
Noxxon Pharma AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Noxxon Pharma AG filed Critical Noxxon Pharma AG
Priority to US10/296,506 priority Critical patent/US20050208487A1/en
Priority to AU2001269039A priority patent/AU2001269039A1/en
Priority to EP01947321A priority patent/EP1283881A2/de
Publication of WO2001092566A2 publication Critical patent/WO2001092566A2/de
Publication of WO2001092566A3 publication Critical patent/WO2001092566A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3204—Inorganic carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/321—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Inorganic Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

Die vorliegende Erfindung betrifft in einem Aspekt eine immobilisierte Nukleinsäure umfassend eine Nukleinsäure und eine Matrix, wobei die Nukleinsäure ein Spiegelmer ist und das Spiegelmer funktional aktiv ist. In einem weiteren Aspekt betrifft die Erfindung eine immobilisierte Nukleinsäure umfassend eine Nukleinsäure und eine Matrix, wobei die Nukleinsäure zumindest über ihr 3'-Ende an die Matrix gekoppelt ist und die Nukleinsäure eine funktionale Nukleinsäure ist. Schließlich betrifft die Erfindung in einem weiteren Aspekt die Verwendung derartiger immobilisierter Nukleinsäuren als Affinitätsliganden beispielsweise in der Chromatographie und in der Apherese.
PCT/EP2001/006014 2000-05-26 2001-05-25 Immobilisierte nukleinsäuren und verwendungen davon Ceased WO2001092566A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/296,506 US20050208487A1 (en) 2000-05-26 2001-05-25 Immobilized nuckeic acids and uses thereof
AU2001269039A AU2001269039A1 (en) 2000-05-26 2001-05-25 Immobilized nucleic acids and uses thereof
EP01947321A EP1283881A2 (de) 2000-05-26 2001-05-25 Immobilisierte nukleinsäuren und verwendungen davon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10026300 2000-05-26
DE10026300.3 2000-05-26

Publications (2)

Publication Number Publication Date
WO2001092566A2 WO2001092566A2 (de) 2001-12-06
WO2001092566A3 true WO2001092566A3 (de) 2002-09-19

Family

ID=7643773

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006014 Ceased WO2001092566A2 (de) 2000-05-26 2001-05-25 Immobilisierte nukleinsäuren und verwendungen davon

Country Status (4)

Country Link
US (1) US20050208487A1 (de)
EP (1) EP1283881A2 (de)
AU (1) AU2001269039A1 (de)
WO (1) WO2001092566A2 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040015107A (ko) 2001-04-30 2004-02-18 아베시아 바이오테크놀러지, 아이엔씨. 고체 지지체 상에 올리뉴클레오티드의 고정화
DE10122862A1 (de) * 2001-05-11 2002-11-21 Noxxon Pharma Ag GnRH bindende Nukleinsäure
DE10258924A1 (de) * 2002-12-17 2004-07-08 Eberhard-Karls-Universität Tübingen Universitätsklinikum Mit die Adhäsion von biologischem Material vermittelnden Substanzen beschichtete Vorrichtung
GB0328448D0 (en) * 2003-12-09 2004-01-14 Univ Leeds Agents for the regulation of transcription via zinc finger proteins
GB0425102D0 (en) * 2004-11-15 2004-12-15 Ciba Spec Chem Water Treat Ltd Polymeric compositions and methods of employing them in papermaking processes
GB0521139D0 (en) 2005-10-18 2005-11-23 Univ Sheffield Therapeutic agent
US20100151465A1 (en) * 2008-03-27 2010-06-17 Jingyue Ju Selective Capture and Release of Analytes
EP2002855B1 (de) * 2007-06-14 2012-07-11 RenApta B.V. Künstliche Niere
US9090663B2 (en) * 2009-04-21 2015-07-28 The Trustees Of Columbia University In The City Of New York Systems and methods for the capture and separation of microparticles
US20110128535A1 (en) * 2009-11-30 2011-06-02 David Eugene Baker Nano-Structured Substrates, Articles, and Methods Thereof
JP5911240B2 (ja) * 2010-10-04 2016-04-27 キヤノン株式会社 多孔質ガラス、その製造方法、光学部材および撮像装置
WO2012055573A1 (en) * 2010-10-29 2012-05-03 Noxxon Pharma Ag Use of hepcidin binding nucleic acids for depletion of hepcidin from the body
WO2013019714A1 (en) 2011-07-29 2013-02-07 The Trustees Of Columbia University In The City Of New York Mems affinity sensor for continuous monitoring of analytes
US10359419B2 (en) * 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
WO2016022696A1 (en) 2014-08-05 2016-02-11 The Trustees Of Columbia University In The City Of New York Method of isolating aptamers for minimal residual disease detection
CN109055490A (zh) * 2018-08-30 2018-12-21 郑州安图生物工程股份有限公司 一种具有核酸洗脱功能的反转录pcr反应液和核酸提取方法
US20220404347A1 (en) * 2021-06-17 2022-12-22 Roche Diagnostics Operations, Inc. Methods for detecting an analyte using structure switching binding agents

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4182750A (en) * 1977-04-21 1980-01-08 Sullivan Thomas E Bloodcompatible functional polymers
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren
DE19745668A1 (de) * 1996-10-17 1998-04-23 Scheller Frieder Prof Definierte Kopplung von biotechnologischen Funktionseinheiten
WO1998051698A1 (en) * 1997-05-14 1998-11-19 Marek Kwiatkowski Synthesis of oligonucleotides

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4182750A (en) * 1977-04-21 1980-01-08 Sullivan Thomas E Bloodcompatible functional polymers
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren
DE19745668A1 (de) * 1996-10-17 1998-04-23 Scheller Frieder Prof Definierte Kopplung von biotechnologischen Funktionseinheiten
WO1998051698A1 (en) * 1997-05-14 1998-11-19 Marek Kwiatkowski Synthesis of oligonucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NOLTE A ET AL: "MIRROR-DESIGN OF L-OLIGONUCLEOTIDE LIGANDS BINDING TO L-ARGININE", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 14, 1 September 1996 (1996-09-01), pages 1116 - 1119, XP002039162, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
US20050208487A1 (en) 2005-09-22
WO2001092566A2 (de) 2001-12-06
EP1283881A2 (de) 2003-02-19
AU2001269039A1 (en) 2001-12-11

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