WO2001094565A3 - FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES - Google Patents

FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES Download PDF

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Publication number
WO2001094565A3
WO2001094565A3 PCT/US2001/018737 US0118737W WO0194565A3 WO 2001094565 A3 WO2001094565 A3 WO 2001094565A3 US 0118737 W US0118737 W US 0118737W WO 0194565 A3 WO0194565 A3 WO 0194565A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
fatty acid
elongase
coa synthase
acid elongase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/018737
Other languages
French (fr)
Other versions
WO2001094565A2 (en
Inventor
Jan G Jaworski
Brenda J Blacklock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miami University
University of Miami
Original Assignee
Miami University
University of Miami
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miami University, University of Miami filed Critical Miami University
Priority to AU6829601A priority Critical patent/AU6829601A/en
Priority to DE60122719T priority patent/DE60122719T2/en
Priority to CA002411247A priority patent/CA2411247A1/en
Priority to AU2001268296A priority patent/AU2001268296B2/en
Priority to EP01946218A priority patent/EP1294898B1/en
Publication of WO2001094565A2 publication Critical patent/WO2001094565A2/en
Publication of WO2001094565A3 publication Critical patent/WO2001094565A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Elongase KCS polypeptides with altered substrate specificity and/or catalytic activity are disclosed. Such elongase KCS polypeptides are effective for producing very long chain fatty acids (VLCFA) fatty acids. Also disclosed are nucleic acids encoding elongase KCS polypeptides, and yeast and plants expressing these polypeptides.
PCT/US2001/018737 2000-06-08 2001-06-08 FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES Ceased WO2001094565A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU6829601A AU6829601A (en) 2000-06-08 2001-06-08 Fatty acid elongase 3-ketoacyl coa synthase polypeptides
DE60122719T DE60122719T2 (en) 2000-06-08 2001-06-08 FATTY ACID ELONGASE 3-ketoacyl-CoA synthase polypeptides
CA002411247A CA2411247A1 (en) 2000-06-08 2001-06-08 Fatty acid elongase 3-ketoacyl coa synthase polypeptides
AU2001268296A AU2001268296B2 (en) 2000-06-08 2001-06-08 Fatty acid elongase 3-ketoacyl coa synthase polypeptides
EP01946218A EP1294898B1 (en) 2000-06-08 2001-06-08 FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21032600P 2000-06-08 2000-06-08
US60/210,326 2000-06-08

Publications (2)

Publication Number Publication Date
WO2001094565A2 WO2001094565A2 (en) 2001-12-13
WO2001094565A3 true WO2001094565A3 (en) 2002-05-02

Family

ID=22782454

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/018737 Ceased WO2001094565A2 (en) 2000-06-08 2001-06-08 FATTY ACID ELONGASE 3-KETOACYL CoA SYNTHASE POLYPEPTIDES

Country Status (7)

Country Link
US (2) US6713664B2 (en)
EP (1) EP1294898B1 (en)
AT (1) ATE338132T1 (en)
AU (2) AU6829601A (en)
CA (1) CA2411247A1 (en)
DE (1) DE60122719T2 (en)
WO (1) WO2001094565A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
CA2547320C (en) * 2003-11-25 2013-07-16 National Research Council Of Canada Fatty acid elongase (fae) genes and their utility in increasing erucic acid and other very long-chain fatty acid proportions in seed oil
GB0603160D0 (en) 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
KR101524398B1 (en) * 2006-03-15 2015-06-04 디에스엠 아이피 어셋츠 비.브이. Polyunsaturated Fatty Acid Production in Heterologous Organisms Using PUFA Polyketide Synthase Systems
CN102741267B (en) * 2009-03-19 2020-06-23 帝斯曼知识产权资产有限公司 Polyunsaturated fatty acid synthase nucleic acid molecules and polypeptides, and compositions, preparation methods and uses thereof
CN102888384A (en) * 2012-10-24 2013-01-23 江苏省中国科学院植物研究所 Rocket salad fatty acid elongase and coding gene thereof
CN103667341B (en) * 2013-11-28 2015-09-02 浙江省农业科学院 A kind of artificial orientation's regulate and control method improving plant long-chain fatty acid content
EP3277827B1 (en) * 2015-04-02 2021-03-24 Biopetrolia AB Fungal cells and methods for production of very long chain fatty acid derived products
CN107674876B (en) * 2016-08-01 2022-06-07 西北农林科技大学 MoKCS gene, enzyme coded by gene and application of gene
CN119285726A (en) * 2024-12-11 2025-01-10 湘湖实验室(农业浙江省实验室) Protein for synthesizing octadecene compounds and method for synthesizing octadecene compounds

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015387A2 (en) * 1993-11-30 1995-06-08 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-coa metabolism
WO1996013582A1 (en) * 1994-10-26 1996-05-09 Dna Plant Technology Corporation Fae1 genes and their uses
WO1998054954A1 (en) * 1997-06-03 1998-12-10 Cargill, Incorporated Fatty acid elongases
WO2001029238A2 (en) * 1999-10-20 2001-04-26 Gesellschaft Für Erwerb Und Verwertung Von Schutzrechten - Gvs Mbh Elongase promoters for the tissue-specific expression of transgenes in plants

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2285970A1 (en) 1997-04-14 1998-10-22 The University Of British Columbia Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
US6051756A (en) * 1998-02-26 2000-04-18 Cargill, Incorporated Particle bombardment transformation of Brassica

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015387A2 (en) * 1993-11-30 1995-06-08 Calgene, Inc. Nucleic acid sequences encoding a plant cytoplasmic protein involved in fatty acyl-coa metabolism
WO1996013582A1 (en) * 1994-10-26 1996-05-09 Dna Plant Technology Corporation Fae1 genes and their uses
WO1998054954A1 (en) * 1997-06-03 1998-12-10 Cargill, Incorporated Fatty acid elongases
WO2001029238A2 (en) * 1999-10-20 2001-04-26 Gesellschaft Für Erwerb Und Verwertung Von Schutzrechten - Gvs Mbh Elongase promoters for the tissue-specific expression of transgenes in plants

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EM_PL EMBL; 24 July 1997 (1997-07-24), CLEMENS S ET AL.: "Brassica napus 3-ketoacyl-CoA synthase (fae1) mRNA, complete cds", XP002191552 *
DATABASE EM_PL EMBL; 4 July 1995 (1995-07-04), JAMES D W JR ET AL.: "Arabidopsis thaliana fatty acid elongase 1 (Fae1) gene, complete cds", XP002191553 *
DOMERGUE F ET AL.: "Seed acyl-CoA elongases: the other system of fatty acid synthesis", OCL-OLEAGINEUX CORPS GRAS LIPIDES, vol. 6, no. 1, January 1999 (1999-01-01), pages 101 - 106, XP001064063 *
JAMES D W JR ET AL.: "Directed tagging of the Arabidopsis FATTY ACID ELONGATION (FAE1) gene with the maize transposon activator", PLANT CELL, vol. 7, March 1995 (1995-03-01), pages 309 - 319, XP002911493 *
MILLAR A ET AL.: "Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme", PLANT JOURNAL, vol. 12, no. 1, 1997, pages 121 - 131, XP002125076, ISSN: 0960-7412 *
POST-BEITTENMILLER D: "Biochemistry and molecular biology of wax production in plants", ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, vol. 47, 1996, pages 405 - 430, XP002125077, ISSN: 1040-2519 *

Also Published As

Publication number Publication date
CA2411247A1 (en) 2001-12-13
WO2001094565A2 (en) 2001-12-13
EP1294898B1 (en) 2006-08-30
EP1294898A2 (en) 2003-03-26
DE60122719D1 (en) 2006-10-12
US20020049994A1 (en) 2002-04-25
ATE338132T1 (en) 2006-09-15
DE60122719T2 (en) 2008-04-17
US6713664B2 (en) 2004-03-30
US20040139498A1 (en) 2004-07-15
AU6829601A (en) 2001-12-17
AU2001268296B2 (en) 2006-05-25

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