WO2001096382A3 - Thermostable cellulase - Google Patents

Thermostable cellulase Download PDF

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Publication number
WO2001096382A3
WO2001096382A3 PCT/IS2001/000012 IS0100012W WO0196382A3 WO 2001096382 A3 WO2001096382 A3 WO 2001096382A3 IS 0100012 W IS0100012 W IS 0100012W WO 0196382 A3 WO0196382 A3 WO 0196382A3
Authority
WO
WIPO (PCT)
Prior art keywords
variant
variants
amino acid
thermostable
thermostable cellulase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IS2001/000012
Other languages
French (fr)
Other versions
WO2001096382A2 (en
Inventor
Kryzysztof B Wicher
Olof Peder Holst
Maher Youssef Abou Hachem
Eva Margareta Nordber Karlsson
Gudmundur O Hreggvidsson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prokaria Ltd
Original Assignee
Prokaria Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ltd filed Critical Prokaria Ltd
Priority to AU2001264212A priority Critical patent/AU2001264212A1/en
Priority to EP01938538A priority patent/EP1297004A2/en
Priority to US10/003,759 priority patent/US6812018B2/en
Publication of WO2001096382A2 publication Critical patent/WO2001096382A2/en
Publication of WO2001096382A3 publication Critical patent/WO2001096382A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Disclosed are variant thermostable cellulases, nucleic acids encoding the variants and methods for producing the variants. The variant thermostable cellulases have the amino acid sequence of a glycosyl hydrolase of family 12 wherein one or more amino acid residues which are not part of the catalytic domain are deleted. In specific embodiments, the variant thermostable cellulase is a variant of Cell2A of Rhodothermus marinus.
PCT/IS2001/000012 2000-06-15 2001-06-15 Thermostable cellulase Ceased WO2001096382A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2001264212A AU2001264212A1 (en) 2000-06-15 2001-06-15 Thermostable cellulase
EP01938538A EP1297004A2 (en) 2000-06-15 2001-06-15 Thermostable cellulase
US10/003,759 US6812018B2 (en) 2000-06-15 2001-10-23 Thermostable cellulase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59488400A 2000-06-15 2000-06-15
US09/594,884 2000-06-15

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US59488400A Continuation-In-Part 2000-06-15 2000-06-15

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/003,759 Continuation-In-Part US6812018B2 (en) 2000-06-15 2001-10-23 Thermostable cellulase

Publications (2)

Publication Number Publication Date
WO2001096382A2 WO2001096382A2 (en) 2001-12-20
WO2001096382A3 true WO2001096382A3 (en) 2002-04-18

Family

ID=24380817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IS2001/000012 Ceased WO2001096382A2 (en) 2000-06-15 2001-06-15 Thermostable cellulase

Country Status (4)

Country Link
US (1) US6812018B2 (en)
EP (1) EP1297004A2 (en)
AU (1) AU2001264212A1 (en)
WO (1) WO2001096382A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030199072A1 (en) * 2002-04-19 2003-10-23 Prokaria, Itd. Crystal and structure of a thermostable glycosol hydrolase and use thereof, and modified proteins
US20050054752A1 (en) * 2003-09-08 2005-03-10 O'brien John P. Peptide-based diblock and triblock dispersants and diblock polymers
US7348172B2 (en) 2004-04-16 2008-03-25 Ab Enzymes Oy Method and DNA constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi
WO2005100557A1 (en) * 2004-04-16 2005-10-27 Ab Enzymes Oy Method and dna constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi
CN101094917B (en) 2004-12-30 2012-04-25 金克克国际有限公司 Novel variant H. jecorina CBH2 cellulase
JP2009511019A (en) * 2005-10-06 2009-03-19 ルシジェン コーポレイション Thermostable viral polymerase and use thereof
US7803601B2 (en) * 2006-10-04 2010-09-28 Board Of Regents, The University Of Texas Production and secretion of glucose in photosynthetic prokaryotes (cyanobacteria)
WO2008042975A2 (en) * 2006-10-04 2008-04-10 Board Of Regents, The University Of Texas System Compositions, methods and systems for producing saccharides in photosynthetic prokaryotes (cyanobacteria)
US20080085536A1 (en) * 2006-10-04 2008-04-10 Board Of Regents, The University Of Texas System Production of Cellulose in Halophilic Photosynthetic Prokaryotes (Cyanobacteria)
US20080124767A1 (en) * 2006-10-04 2008-05-29 Board Of Regents, The University Of Texas At Austin Production and Secretion of Sucrose in Photosynthetic Prokaryotes (Cyanobacteria)
AU2007318868B2 (en) * 2006-11-06 2012-03-15 Council Of Scientific & Industrial Research A recombinant meso-active thermo-stable protein and the process of design and biosynthesis thereof
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
RU2565550C2 (en) 2010-09-24 2015-10-20 Те Борд Оф Трастиз Оф Те Лилэнд Стэнфорд Джуниор Юниверсити Direct capture, amplification and sequencing of target dna using immobilised primers
EP2785853A2 (en) 2011-12-02 2014-10-08 BP Corporation North America Inc. Compositions and methods for biomass liquefaction
CN103756979B (en) * 2012-05-10 2015-07-08 江南大学 Method for improving thermal stability of enzyme
CN103805579B (en) * 2014-02-19 2016-05-18 江南大学 A kind of heat-staple amylase mutant and its preparation method and application
JP6268486B2 (en) 2014-08-04 2018-01-31 本田技研工業株式会社 Super thermostable endoglucanase belonging to GH family 12
JP6315807B2 (en) 2014-08-04 2018-04-25 本田技研工業株式会社 Super thermostable endoglucanase belonging to GH family 12
JP6552098B2 (en) 2015-08-20 2019-07-31 本田技研工業株式会社 Super thermostable endoglucanase
JP6244597B2 (en) * 2015-08-21 2017-12-13 本田技研工業株式会社 Super thermostable endoglucanase

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044361A1 (en) * 1996-05-22 1997-11-27 Recombinant Biocatalysis, Inc. Endoglucanases
WO1998033895A1 (en) * 1997-01-31 1998-08-06 Novo Nordisk A/S A thermostable endo-beta-1,4-glucanase
WO1999031255A2 (en) * 1997-12-16 1999-06-24 Genencor International, Inc. Novel egiii-like enzymes, dna encoding such enzymes and methods for producing such enzymes
WO2000009707A1 (en) * 1998-06-24 2000-02-24 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
WO2000037614A2 (en) * 1998-12-18 2000-06-29 Genencor International, Inc. Variants of egiii-like cellulase and compositions thereof

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Publication number Priority date Publication date Assignee Title
US4966850A (en) 1987-01-21 1990-10-30 Forintek Canada Corp. Production of thermostable xylanase and cellulase
US4795101A (en) 1987-05-13 1989-01-03 Genencor, Inc. Use of cellulase in a method of wet milling a starch-containing grain
US4832864A (en) 1987-09-15 1989-05-23 Ecolab Inc. Compositions and methods that introduce variations in color density into cellulosic fabrics, particularly indigo dyed denim
LT3208B (en) 1992-04-10 1995-03-27 Ssv Dev Oy Enzyme products for use in the improvement of feed value and conservation of fibrous crops
US5792641A (en) 1992-10-06 1998-08-11 Novo Nordisk A/S Cellulase variants and detergent compositions containing cellulase variants
US5883066A (en) 1993-06-28 1999-03-16 The Procter & Gamble Company Liquid detergent compositions containing cellulase and amine
US5445957A (en) 1994-02-10 1995-08-29 Triarco Industries, Inc. Enzyme food supplement composition containing beta-fructofuranosidase, cellulase and hemicellulase
US5677151A (en) 1994-06-24 1997-10-14 Cornell Research Foundation, Inc. Thermostable cellulase from a thermomonospora gene
US5525193A (en) 1994-10-26 1996-06-11 Novo Nordisk A/S Use of monocomponent cellulase for removing inks, coatings, and toners from printed paper
US5919271A (en) 1994-12-31 1999-07-06 Procter & Gamble Company Detergent composition comprising cellulase enzyme and nonionic cellulose ether
DE19515072A1 (en) 1995-04-28 1996-10-31 Cognis Bio Umwelt Detergent containing cellulase
US5700686A (en) 1995-06-06 1997-12-23 Iogen Corporation Protease-treated and purified cellulase compositions and methods for reducing backstaining during enzymatic stonewashing
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
MX9801889A (en) 1995-09-20 1998-05-31 Genencor Int Protease modified cellulase compositions.
AU5713896A (en) 1996-03-12 1997-10-01 Genencor International, Inc. Alkaline cellulase and method of producing same
EP0927756B1 (en) 1996-03-14 2006-07-26 Japan ,represented by Director General of Agency of Industrial Science and Technology Protein having cellulase activities and process for producing the same
WO1998001569A1 (en) 1996-07-08 1998-01-15 Rijkslandbouwuniversiteit Wageningen Cellulases
WO1998006892A1 (en) 1996-08-16 1998-02-19 International Paper Company Enzymatic freeness enhancement
BR9711479B1 (en) 1996-09-17 2009-08-11 cellulase variant having increased resistance to anion voltage.
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Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044361A1 (en) * 1996-05-22 1997-11-27 Recombinant Biocatalysis, Inc. Endoglucanases
WO1998033895A1 (en) * 1997-01-31 1998-08-06 Novo Nordisk A/S A thermostable endo-beta-1,4-glucanase
WO1999031255A2 (en) * 1997-12-16 1999-06-24 Genencor International, Inc. Novel egiii-like enzymes, dna encoding such enzymes and methods for producing such enzymes
WO2000009707A1 (en) * 1998-06-24 2000-02-24 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
WO2000037614A2 (en) * 1998-12-18 2000-06-29 Genencor International, Inc. Variants of egiii-like cellulase and compositions thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
K B WICHER ET AL: "Deletion of a cytotoxic, N-terminal putative signal peptide results in a significant increase in production yields in Esterichia coli and improved specific activity of Cel12A from Rhodothermus marinus", APPL. MICROBIOL BIOTECHNOL, vol. 55, 2001, pages 578 - 584, XP002902256 *
S HALLDORSDOTTIR ET AL: "Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12", APPL. MICROBIOL BIOTECHNOL, vol. 49, 1998, pages 277 - 284, XP002902257 *

Also Published As

Publication number Publication date
US6812018B2 (en) 2004-11-02
WO2001096382A2 (en) 2001-12-20
EP1297004A2 (en) 2003-04-02
AU2001264212A1 (en) 2001-12-24
US20020102699A1 (en) 2002-08-01

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