WO2002000938A3 - Methods and compositions for transcription-based nucleic acid amplification - Google Patents

Methods and compositions for transcription-based nucleic acid amplification Download PDF

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Publication number
WO2002000938A3
WO2002000938A3 PCT/US2001/020660 US0120660W WO0200938A3 WO 2002000938 A3 WO2002000938 A3 WO 2002000938A3 US 0120660 W US0120660 W US 0120660W WO 0200938 A3 WO0200938 A3 WO 0200938A3
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WO
WIPO (PCT)
Prior art keywords
amplification
methods
transcription
module
rna transcripts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/020660
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French (fr)
Other versions
WO2002000938A9 (en
WO2002000938A2 (en
Inventor
Nurith Kurn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nugen Technologies Inc
Original Assignee
Nugen Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2001271595A priority Critical patent/AU2001271595A1/en
Priority to AT01950624T priority patent/ATE455186T1/en
Priority to DE60141087T priority patent/DE60141087D1/en
Priority to CA002412721A priority patent/CA2412721A1/en
Priority to EP01950624A priority patent/EP1356094B1/en
Priority to MXPA02012735A priority patent/MXPA02012735A/en
Application filed by Nugen Technologies Inc filed Critical Nugen Technologies Inc
Priority to JP2002506251A priority patent/JP2004513617A/en
Publication of WO2002000938A2 publication Critical patent/WO2002000938A2/en
Anticipated expiration legal-status Critical
Publication of WO2002000938A9 publication Critical patent/WO2002000938A9/en
Publication of WO2002000938A3 publication Critical patent/WO2002000938A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification for the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.
PCT/US2001/020660 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification Ceased WO2002000938A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AT01950624T ATE455186T1 (en) 2000-06-26 2001-06-26 METHODS AND COMPOSITIONS FOR TRANSCRIPTION-BASED DUPLICATION OF NUCLEIC ACIDS
DE60141087T DE60141087D1 (en) 2000-06-26 2001-06-26 METHODS AND COMPOSITIONS FOR TRANSCRIPTION-REPRODUCTIVE MACHINING OF NUCLEIC ACIDS
CA002412721A CA2412721A1 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
EP01950624A EP1356094B1 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
MXPA02012735A MXPA02012735A (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification.
AU2001271595A AU2001271595A1 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification
JP2002506251A JP2004513617A (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US21390800P 2000-06-26 2000-06-26
US60/213,908 2000-06-26
US27774801P 2001-03-21 2001-03-21
US60/277,748 2001-03-21

Publications (3)

Publication Number Publication Date
WO2002000938A2 WO2002000938A2 (en) 2002-01-03
WO2002000938A9 WO2002000938A9 (en) 2003-07-03
WO2002000938A3 true WO2002000938A3 (en) 2003-08-28

Family

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Family Applications (1)

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PCT/US2001/020660 Ceased WO2002000938A2 (en) 2000-06-26 2001-06-26 Methods and compositions for transcription-based nucleic acid amplification

Country Status (10)

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US (3) US6686156B2 (en)
EP (1) EP1356094B1 (en)
JP (1) JP2004513617A (en)
CN (1) CN1498274A (en)
AT (1) ATE455186T1 (en)
AU (1) AU2001271595A1 (en)
CA (1) CA2412721A1 (en)
DE (1) DE60141087D1 (en)
MX (1) MXPA02012735A (en)
WO (1) WO2002000938A2 (en)

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US7351557B2 (en) 2001-03-09 2008-04-01 Nugen Technologies, Inc. Amplification of RNA sequences using composite RNA-DNA primers and strand displacement
US7771946B2 (en) 2001-03-09 2010-08-10 Nugen Technologies, Inc. Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences
US7771934B2 (en) 2000-12-13 2010-08-10 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8465950B2 (en) 2003-04-14 2013-06-18 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US9074246B2 (en) 2010-01-25 2015-07-07 Rd Biosciences, Inc. Self-folding amplification of target nucleic acid
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US9650628B2 (en) 2012-01-26 2017-05-16 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library regeneration
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
US9957549B2 (en) 2012-06-18 2018-05-01 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US11028430B2 (en) 2012-07-09 2021-06-08 Nugen Technologies, Inc. Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing

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