WO2002004620A3 - Verfahren zur isolierung von nukleinsäuren - Google Patents

Verfahren zur isolierung von nukleinsäuren Download PDF

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Publication number
WO2002004620A3
WO2002004620A3 PCT/EP2001/008066 EP0108066W WO0204620A3 WO 2002004620 A3 WO2002004620 A3 WO 2002004620A3 EP 0108066 W EP0108066 W EP 0108066W WO 0204620 A3 WO0204620 A3 WO 0204620A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
isolating nucleic
solution
relates
alkali halides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/008066
Other languages
English (en)
French (fr)
Other versions
WO2002004620A2 (de
Inventor
Martin Weber
Thorsten Singer
Sarah Cosaert
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to EP01971766A priority Critical patent/EP1299531A2/de
Priority to US10/332,667 priority patent/US20040091875A1/en
Priority to JP2002509474A priority patent/JP2004502458A/ja
Publication of WO2002004620A2 publication Critical patent/WO2002004620A2/de
Publication of WO2002004620A3 publication Critical patent/WO2002004620A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Isolierung von Nucleinsäuren aus einer Lösung, wobei die Nukleinsäuren an einer SiO2-haltigen Oberfläche in Gegenwart von Alkalihalogeniden und Alkohol absorbiert werden. Ferner betrifft die Erfindung die Verwendung einer Pufferlösung enthaltend Alkalihalogenide zur Isolierung von Nukleinsäuren an einem SiO2-haltigen Träger sowie ein Kit zur Durchführung eines Verfahrens zur Isolierung von Nukleinsäuren aus einer Lösung.
PCT/EP2001/008066 2000-07-12 2001-07-12 Verfahren zur isolierung von nukleinsäuren Ceased WO2002004620A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01971766A EP1299531A2 (de) 2000-07-12 2001-07-12 Verfahren zur isolierung von nukleinsäuren
US10/332,667 US20040091875A1 (en) 2000-07-12 2001-07-12 Method for isolating nucleic acids
JP2002509474A JP2004502458A (ja) 2000-07-12 2001-07-12 核酸の単離方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10033991.3 2000-07-12
DE10033991A DE10033991A1 (de) 2000-07-12 2000-07-12 Verfahren zur Isolierung von Nukleinsäuren

Publications (2)

Publication Number Publication Date
WO2002004620A2 WO2002004620A2 (de) 2002-01-17
WO2002004620A3 true WO2002004620A3 (de) 2002-07-18

Family

ID=7648743

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008066 Ceased WO2002004620A2 (de) 2000-07-12 2001-07-12 Verfahren zur isolierung von nukleinsäuren

Country Status (5)

Country Link
US (1) US20040091875A1 (de)
EP (1) EP1299531A2 (de)
JP (1) JP2004502458A (de)
DE (1) DE10033991A1 (de)
WO (1) WO2002004620A2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
DE10253351B4 (de) * 2002-11-08 2007-02-22 InViTek Gesellschaft für Biotechnik & Biodesign mbH Neuartige Pufferfomulierungen zur Isolierung, Reinigung und Rückgewinnung lang- und kurzkettiger Nukleinsäuren
EP1654361B2 (de) † 2003-07-25 2023-01-18 Life Technologies Corporation Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe
JP2008220380A (ja) * 2003-10-31 2008-09-25 Fujifilm Corp 核酸の分離精製方法
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
DE102005047736B4 (de) 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
DE102005059217B4 (de) 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
JP5463492B2 (ja) * 2008-10-08 2014-04-09 島根県 微生物細胞からのプラスミドｄｎａ抽出法
CN103173432B (zh) 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
DE102012012523B4 (de) 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512767A1 (de) * 1991-05-03 1992-11-11 Becton, Dickinson and Company Festphase-Extraktionsreinigung von DNA
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
WO1996021729A1 (en) * 1995-01-13 1996-07-18 Vical Incorporated Process for reducing rna concentration in a mixture of biological material using diatomaceous earth
WO1999054340A1 (en) * 1998-04-22 1999-10-28 Promega Corporation Endotoxin reduction in nucleic acid purification
WO2000034463A1 (de) * 1998-12-04 2000-06-15 Invitek Gmbh Formulierungen und verfahren zur isolierung von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien und nachfolgende komplexe genanalytik

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
JP3256238B2 (ja) * 1994-02-11 2002-02-12 クイアジェン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング 二本鎖／単鎖核酸構造物の分離方法
JP4196227B2 (ja) * 1997-05-20 2008-12-17 東洋紡績株式会社 核酸またはタンパク質抽出用シリカ粒子組成物
JPH11196869A (ja) * 1998-01-19 1999-07-27 Toyobo Co Ltd リボ核酸の単離方法
ATE521703T1 (de) * 1999-05-14 2011-09-15 Promega Corp Zellkonzentration und lysatklärung mittels paramagnetischen partikeln

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512767A1 (de) * 1991-05-03 1992-11-11 Becton, Dickinson and Company Festphase-Extraktionsreinigung von DNA
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
WO1996021729A1 (en) * 1995-01-13 1996-07-18 Vical Incorporated Process for reducing rna concentration in a mixture of biological material using diatomaceous earth
WO1999054340A1 (en) * 1998-04-22 1999-10-28 Promega Corporation Endotoxin reduction in nucleic acid purification
WO2000034463A1 (de) * 1998-12-04 2000-06-15 Invitek Gmbh Formulierungen und verfahren zur isolierung von nukleinsäuren aus beliebigen komplexen ausgangsmaterialien und nachfolgende komplexe genanalytik

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NEUDECKER FRANK ET AL: "High-throughput method for isolating plasmid DNA with reduced lipopolysaccharide content.", BIOTECHNIQUES, vol. 28, no. 1, January 2000 (2000-01-01), pages 107 - 109, XP002158615, ISSN: 0736-6205 *

Also Published As

Publication number Publication date
DE10033991A1 (de) 2002-01-24
JP2004502458A (ja) 2004-01-29
WO2002004620A2 (de) 2002-01-17
US20040091875A1 (en) 2004-05-13
EP1299531A2 (de) 2003-04-09

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