WO2002004657A3 - Screening method - Google Patents

Screening method Download PDF

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Publication number
WO2002004657A3
WO2002004657A3 PCT/GB2001/003101 GB0103101W WO0204657A3 WO 2002004657 A3 WO2002004657 A3 WO 2002004657A3 GB 0103101 W GB0103101 W GB 0103101W WO 0204657 A3 WO0204657 A3 WO 0204657A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
apoptopic
exposing
identifying
state
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/003101
Other languages
French (fr)
Other versions
WO2002004657A2 (en
Inventor
Tom Cotter
Ian Hayes
Finbarr Murphy
Liam Seery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EiRx Therapeutics Ltd
Original Assignee
EiRx Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0016692.6A external-priority patent/GB0016692D0/en
Application filed by EiRx Therapeutics Ltd filed Critical EiRx Therapeutics Ltd
Priority to EP01947681A priority Critical patent/EP1299558B1/en
Priority to DE60119524T priority patent/DE60119524T2/en
Priority to AU2001269326A priority patent/AU2001269326A1/en
Publication of WO2002004657A2 publication Critical patent/WO2002004657A2/en
Anticipated expiration legal-status Critical
Publication of WO2002004657A3 publication Critical patent/WO2002004657A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2510/00—Detection of programmed cell death, i.e. apoptosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to a method for identifying a gene product which modulates the transition of a cell between a non-apoptopic state and an apoptopic state, comprising the steps of: (a) exposing the cell to an inhibitor of GM-CSF mediated inhibition of apoptosis; and (b) exposing the cell to one or more agents which increase tyrosine phosphorylation; and (c) placing the cell in conditions which permit it to undergo spontaneous apoptosis; and (d) monitoring the level(s) of expression of the one or more gene products in the cell; and (e) identifying gene product(s) whose expression has been increased, decreased or modified as a result of performing steps (a) to (d).
PCT/GB2001/003101 2000-07-07 2001-07-09 Screening method Ceased WO2002004657A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01947681A EP1299558B1 (en) 2000-07-07 2001-07-09 Screening method
DE60119524T DE60119524T2 (en) 2000-07-07 2001-07-09 SCREENING PROCESS
AU2001269326A AU2001269326A1 (en) 2000-07-07 2001-07-09 Screening method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0016692.6A GB0016692D0 (en) 2000-07-07 2000-07-07 Method
GB0016692.6 2000-07-07
US25445900P 2000-12-08 2000-12-08
US60/254,459 2000-12-08

Publications (2)

Publication Number Publication Date
WO2002004657A2 WO2002004657A2 (en) 2002-01-17
WO2002004657A3 true WO2002004657A3 (en) 2003-01-16

Family

ID=26244614

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/003101 Ceased WO2002004657A2 (en) 2000-07-07 2001-07-09 Screening method

Country Status (5)

Country Link
EP (1) EP1299558B1 (en)
AT (1) ATE325889T1 (en)
AU (1) AU2001269326A1 (en)
DE (1) DE60119524T2 (en)
WO (1) WO2002004657A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002352459A1 (en) * 2001-12-20 2003-07-09 Eirx Therapeutics Limited Early stage redox-related apoptosis modulator-2 (esram-2)
WO2004065959A2 (en) * 2003-01-23 2004-08-05 Eirx Therapeutics Limited Kinases and gpcrs involved in apoptosis
GB0305267D0 (en) * 2003-03-07 2003-04-09 Eirx Therapeutics Ltd Proteins involved in apoptosis
WO2007002088A2 (en) * 2005-06-20 2007-01-04 Exelixis, Inc. Psat1s as modifiers of the pten/akt pathway and methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0926242A1 (en) * 1996-12-02 1999-06-30 Ajinomoto Co., Inc. Gliotoxin derivatives and anticancer agent comprising the same
WO1999032514A2 (en) * 1997-12-19 1999-07-01 Warner-Lambert Company Sag: sensitive to apoptosis gene

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0926242A1 (en) * 1996-12-02 1999-06-30 Ajinomoto Co., Inc. Gliotoxin derivatives and anticancer agent comprising the same
WO1999032514A2 (en) * 1997-12-19 1999-07-01 Warner-Lambert Company Sag: sensitive to apoptosis gene

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
COXON ANGELA ET AL: "Cytokine-activated endothelial cells delay neutrophil apoptosis in vitro and in vivo: A role for granulocyte/macrophage colony-stimulating factor", JOURNAL OF EXPERIMENTAL MEDICINE, TOKYO, JP, vol. 190, no. 7, 4 October 1999 (1999-10-04), pages 923 - 933, XP002194526, ISSN: 0022-1007 *
MCCUBREY J.A:, MAY W. STRATFORD, DURINO V. MUFSON A.: "serine/threonine phosphorylation in cytokine signal transduction", LEUKEMIA, vol. 14, no. 1, January 2000 (2000-01-01), pages 9 - 21, XP002209543 *
P. WEINMANN, P. GAEHTGENS, B. WALZOG: "Bcl-X1 and Bax-alpha mediated regulation of Apoptosis of human neutrophils via caspase-3", BLOOD, vol. 93, no. 9, 1999, pages 3106 - 3115, XP002209559 *
S.J. WILSON, B.A. LEONE, D. ANDERSON, A. MANNING, S.T. HOLGATE: "Immunohistochemical analysis of the activation of NF-kappaB and expression of associated cytokines and adhesion molecules in human models of allergic inflammation", JOURNAL OF PATHOLOGY, vol. 189, 1999, pages 265 - 272, XP002209544 *
W. YUGANG, L. HONTAO, Z. YINGMEI, M. DALONG: "cDNA cloning and expresion of an apoptosis-related gene, human TFAR15 gene", SCIENCE IN CHINA SERIES C LIFE SCIENCES, vol. 42, no. 3, 1999, pages 323 - 329, XP002209542 *
Y. WANG, T. REA, J. BIAN, S.GRAY, Y. SUN: "Identification of genes responsive to etoposide-induced apoptosis: application of DNA chip technology", FEBS, vol. 445, 1999, pages 269 - 273, XP002209541 *

Also Published As

Publication number Publication date
EP1299558A2 (en) 2003-04-09
ATE325889T1 (en) 2006-06-15
DE60119524D1 (en) 2006-06-14
WO2002004657A2 (en) 2002-01-17
EP1299558B1 (en) 2006-05-10
DE60119524T2 (en) 2007-05-03
AU2001269326A1 (en) 2002-01-21

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