WO2002004680A3 - Real-time sequence determination - Google Patents

Real-time sequence determination Download PDF

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Publication number
WO2002004680A3
WO2002004680A3 PCT/US2001/021811 US0121811W WO0204680A3 WO 2002004680 A3 WO2002004680 A3 WO 2002004680A3 US 0121811 W US0121811 W US 0121811W WO 0204680 A3 WO0204680 A3 WO 0204680A3
Authority
WO
WIPO (PCT)
Prior art keywords
real
time sequence
sequence determination
methodology
dntps
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/021811
Other languages
French (fr)
Other versions
WO2002004680A2 (en
Inventor
Susan H Hardin
James M Briggs
Shiao-Chun Tu
Xiaolian Gao
Richard Willson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Visigen Biotechnologies Inc
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Visigen Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22807697&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2002004680(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Visigen Biotechnologies Inc filed Critical Visigen Biotechnologies Inc
Priority to JP2002509533A priority Critical patent/JP2004513619A/en
Priority to EP01961632A priority patent/EP1368460B1/en
Priority to AU2001282881A priority patent/AU2001282881B2/en
Priority to DE60131194T priority patent/DE60131194T2/en
Priority to AU8288101A priority patent/AU8288101A/en
Priority to CA002415897A priority patent/CA2415897A1/en
Publication of WO2002004680A2 publication Critical patent/WO2002004680A2/en
Anticipated expiration legal-status Critical
Publication of WO2002004680A3 publication Critical patent/WO2002004680A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Soil Working Implements (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Oscillators With Electromechanical Resonators (AREA)
  • Error Detection And Correction (AREA)
  • Color Television Systems (AREA)
  • Saccharide Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
PCT/US2001/021811 2000-07-07 2001-07-09 Real-time sequence determination Ceased WO2002004680A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2002509533A JP2004513619A (en) 2000-07-07 2001-07-09 Real-time sequencing
EP01961632A EP1368460B1 (en) 2000-07-07 2001-07-09 Real-time sequence determination
AU2001282881A AU2001282881B2 (en) 2000-07-07 2001-07-09 Real-time sequence determination
DE60131194T DE60131194T2 (en) 2000-07-07 2001-07-09 SEQUENCE PROVISION IN REAL TIME
AU8288101A AU8288101A (en) 2000-07-07 2001-07-09 Real-time sequence determination
CA002415897A CA2415897A1 (en) 2000-07-07 2001-07-09 Real-time sequence determination

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21659400P 2000-07-07 2000-07-07
US60/216,594 2000-07-07

Publications (2)

Publication Number Publication Date
WO2002004680A2 WO2002004680A2 (en) 2002-01-17
WO2002004680A3 true WO2002004680A3 (en) 2003-10-16

Family

ID=22807697

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021811 Ceased WO2002004680A2 (en) 2000-07-07 2001-07-09 Real-time sequence determination

Country Status (9)

Country Link
US (22) US20030064366A1 (en)
EP (3) EP1975251A3 (en)
JP (3) JP2004513619A (en)
CN (2) CN101525660A (en)
AT (1) ATE377093T1 (en)
AU (2) AU2001282881B2 (en)
CA (1) CA2415897A1 (en)
DE (1) DE60131194T2 (en)
WO (1) WO2002004680A2 (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7323320B2 (en) 2002-09-12 2008-01-29 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9365838B2 (en) 2009-03-27 2016-06-14 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10246744B2 (en) 2016-08-15 2019-04-02 Omniome, Inc. Method and system for sequencing nucleic acids
US11705217B2 (en) 2008-03-28 2023-07-18 Pacific Biosciences Of California, Inc. Sequencing using concatemers of copies of sense and antisense strands

Families Citing this family (827)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9213691D0 (en) * 1992-06-27 1992-08-12 Hurlstone Gary Personal alarm torch
US5823720A (en) * 1996-02-16 1998-10-20 Bitmoore High precision cutting tools
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
JP4638043B2 (en) 1998-12-14 2011-02-23 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド Systems and methods for sequencing single molecule nucleic acids by polymerase synthesis
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
ATE377093T1 (en) * 2000-07-07 2007-11-15 Visigen Biotechnologies Inc REAL-TIME SEQUENCE DETERMINATION
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7727722B2 (en) * 2001-08-29 2010-06-01 General Electric Company Ligation amplification
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US20050009124A1 (en) * 2001-11-26 2005-01-13 Echelon Biosciences Incorporated Assays for detection of phosphoinositide kinase and phosphatase activity
US20040014096A1 (en) * 2002-04-12 2004-01-22 Stratagene Dual-labeled nucleotides
ATE547535T1 (en) * 2002-08-29 2012-03-15 Ge Healthcare Bio Sciences NUCLEOSIDE POLYPHOSPHATES WITH BLOCKED TERMINAL PHOSPHATE
US7910295B2 (en) * 2002-11-14 2011-03-22 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimarker quantitative RT-PCR
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
JP4896708B2 (en) * 2003-02-05 2012-03-14 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Terminal phosphate-labeled nucleotides with novel linkers
JP2006513705A (en) * 2003-02-05 2006-04-27 アマシャム・バイオサイエンス・コーポレイション Nucleic acid growth
WO2004071948A2 (en) * 2003-02-10 2004-08-26 Reveo, Inc. Micro-nozzle, nano-nozzle, manufacturing methods therefor, applications therefor
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (en) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
US20060062531A1 (en) * 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7405434B2 (en) * 2004-11-16 2008-07-29 Cornell Research Foundation, Inc. Quantum dot conjugates in a sub-micrometer fluidic channel
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
JP2006197832A (en) * 2005-01-19 2006-08-03 Tohoku Univ Universal substrate affinity detection method for ABC transporter mutated with cysteine residues using environmentally sensitive fluorescent probe
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP1846758A2 (en) * 2005-02-09 2007-10-24 Pacific Biosciences of California, Inc. Nucleotide compositions and uses thereof
US20100203504A1 (en) * 2005-07-07 2010-08-12 Yoichi Katsumoto Substance-Information Acquisition Method Using Evanescent Light Beam, Substance-Information Measurement Apparatus, Base-Sequence Determination Method and Base-Sequence Determination Apparatus
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007041621A2 (en) * 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
US7825037B2 (en) * 2005-10-17 2010-11-02 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US10060904B1 (en) 2005-10-17 2018-08-28 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US9156004B2 (en) 2005-10-17 2015-10-13 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US8017331B2 (en) * 2005-11-04 2011-09-13 Mannkind Corporation IRE-1α substrates
ATE481505T1 (en) 2005-12-12 2010-10-15 Us Gov Health & Human Serv NUCLEIC ACID SEQUENCING PROBE AND METHOD OF USE
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US8262900B2 (en) * 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2639578B1 (en) 2006-12-14 2016-09-14 Life Technologies Corporation Apparatus for measuring analytes using large scale fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2126072A4 (en) * 2007-02-21 2011-07-13 Life Technologies Corp Materials and methods for single molecule nucleic acid sequencing
EP3543357A1 (en) 2007-05-08 2019-09-25 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
WO2008147879A1 (en) * 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8703422B2 (en) 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US8182993B2 (en) * 2007-06-06 2012-05-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2009046094A1 (en) 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
JP2011505119A (en) * 2007-10-22 2011-02-24 ライフ テクノロジーズ コーポレーション Method and system for obtaining ordered and fragmented sequence fragments along a nucleic acid molecule
GB0721340D0 (en) * 2007-10-30 2007-12-12 Isis Innovation Polymerase-based single-molecule DNA sequencing
WO2009117031A2 (en) * 2007-12-18 2009-09-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
CN101519698B (en) * 2008-03-10 2013-05-22 周国华 Method for quantitatively measuring nucleic acid with sequence tags
JP4586081B2 (en) * 2008-03-31 2010-11-24 株式会社日立ハイテクノロジーズ Fluorescence analyzer
US20090269746A1 (en) * 2008-04-25 2009-10-29 Gil Atzmon Microsequencer-whole genome sequencer
US8148503B2 (en) 2008-06-11 2012-04-03 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in DNA sequencing
JP5268444B2 (en) * 2008-06-23 2013-08-21 株式会社日立ハイテクノロジーズ Single molecule real-time sequence device, nucleic acid analyzer, and single molecule real-time sequence method
CN102203282B (en) 2008-06-25 2014-04-30 生命技术公司 Methods and apparatus for measuring analytes using large scale FET arrays
US20110281740A1 (en) * 2008-06-30 2011-11-17 Joseph Beechem Methods for Real Time Single Molecule Sequencing
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100035252A1 (en) * 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8882980B2 (en) * 2008-09-03 2014-11-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
WO2010039189A2 (en) * 2008-09-23 2010-04-08 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
WO2010062775A2 (en) 2008-11-03 2010-06-03 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
EP2607496B1 (en) 2008-12-23 2014-07-16 Illumina, Inc. Methods useful in nucleic acid sequencing protocols
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
EP2411536B1 (en) * 2009-03-27 2014-09-17 Nabsys, Inc. Methods for analyzing biomolecules and probes bound thereto
WO2010129019A2 (en) 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
US20100279882A1 (en) * 2009-05-01 2010-11-04 Mostafa Ronaghi Sequencing methods
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20100311144A1 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
WO2010144151A2 (en) * 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Single-molecule real-time analysis of protein synthesis
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
US20110040081A1 (en) * 2009-08-14 2011-02-17 Epicentre Technologies Corporation METHODS, COMPOSITIONS, AND KITS FOR GENERATING rRNA-DEPLETED SAMPLES OR ISOLATING rRNA FROM SAMPLES
EP2475787A2 (en) * 2009-09-07 2012-07-18 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
WO2011078897A1 (en) * 2009-09-15 2011-06-30 Life Technologies Corporation Improved sequencing methods, compositions, systems, kits and apparatuses
EP2483680A4 (en) 2009-09-30 2014-01-01 Quantapore Inc Ultrafast sequencing of biological polymers using a labeled nanopore
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
WO2011090710A2 (en) * 2009-12-28 2011-07-28 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Composite probes and use thereof in super resolution methods
WO2011093939A1 (en) 2010-02-01 2011-08-04 Illumina, Inc. Focusing methods and optical systems and assemblies using the same
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
DE202011003570U1 (en) 2010-03-06 2012-01-30 Illumina, Inc. Systems and apparatus for detecting optical signals from a sample
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
SG10201503540QA (en) * 2010-05-06 2015-06-29 Ibis Biosciences Inc Integrated sample preparation systems and stabilized enzyme mixtures
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
TW201716791A (en) 2010-06-30 2017-05-16 生命技術公司 Apparatus and method for testing an ion sensing field effect transistor (ISFET) array
EP2598804B1 (en) 2010-06-30 2021-10-20 Life Technologies Corporation Method to generate an output signal in an isfet-array
WO2012003363A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Ion-sensing charge-accumulation circuits and methods
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
TWI527245B (en) 2010-07-03 2016-03-21 生命技術公司 Chemical sensor with micro-doped bungee
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US8889886B2 (en) 2010-08-25 2014-11-18 Pacific Biosciences, Inc. Cyanine dyes
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
CA2811333C (en) 2010-09-16 2020-05-12 Gen-Probe Incorporated Capture probes immobilizable via l-nucleotide tail
CA2811294C (en) 2010-09-16 2016-06-28 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
EP2619564B1 (en) 2010-09-24 2016-03-16 Life Technologies Corporation Matched pair transistor circuits
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012055929A1 (en) 2010-10-26 2012-05-03 Illumina, Inc. Sequencing methods
AU2011323478B2 (en) 2010-11-01 2015-09-10 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2640849B1 (en) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
EP2643484A4 (en) 2010-11-22 2014-04-16 Univ California METHODS OF IDENTIFYING A CELLULAR TRANSCRIPT FROM RNA
SG191409A1 (en) 2010-12-27 2013-08-30 Ibis Biosciences Inc Nucleic acid sample preparation methods and compositions
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
WO2012106081A2 (en) 2011-01-31 2012-08-09 Illumina, Inc. Methods for reducing nucleic acid damage
WO2012104851A1 (en) 2011-01-31 2012-08-09 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US9868945B2 (en) 2011-02-08 2018-01-16 Life Technologies Corporation Linking methods, compositions, systems, kits and apparatuses
WO2012109574A2 (en) 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
ES2538694T3 (en) * 2011-04-01 2015-06-23 F. Hoffmann-La Roche Ag Variants of the T7 RNA polymerase with Cysteine-Serine substitutions
EP2694709B1 (en) 2011-04-08 2016-09-14 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
US20130040365A1 (en) 2011-08-10 2013-02-14 Life Technologies Corporation Polymerase compositions, methods of making and using same
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
CN104024269B (en) 2011-09-13 2017-05-24 激光基因公司 5-methoxy, 3'-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
US9164053B2 (en) * 2011-09-26 2015-10-20 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
US10152569B2 (en) 2011-09-26 2018-12-11 Gen-Probe Incorporated Algorithms for sequence determinations
WO2013063308A1 (en) 2011-10-25 2013-05-02 University Of Massachusetts An enzymatic method to enrich for capped rna, kits for performing same, and compositions derived therefrom
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
JP5863396B2 (en) * 2011-11-04 2016-02-16 株式会社日立製作所 DNA sequence decoding system, DNA sequence decoding method and program
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
DK2788499T3 (en) 2011-12-09 2016-03-21 Illumina Inc Enhanced root for polymer tags
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
US9334491B2 (en) 2011-12-22 2016-05-10 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
ES2645418T3 (en) 2011-12-22 2017-12-05 Ibis Biosciences, Inc. Amplification of a sequence of a ribonucleic acid
EP3211100A1 (en) 2011-12-22 2017-08-30 Ibis Biosciences, Inc. Amplification primers and methods
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
EP2802673B1 (en) 2012-01-09 2019-07-03 Oslo Universitetssykehus HF Methods and biomarkers for analysis of colorectal cancer
CN108611398A (en) 2012-01-13 2018-10-02 Data生物有限公司 Genotyping is carried out by new-generation sequencing
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
EP2809807B1 (en) 2012-02-01 2019-04-10 Gen-Probe Incorporated Asymmetric hairpin target capture oligomers
CN107326085B (en) * 2012-02-03 2022-05-03 加州理工学院 Encoding and decoding of signals in multiplexed biochemical assays
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
NO2694769T3 (en) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
CA3138752C (en) 2012-04-03 2024-02-06 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
ES2833524T3 (en) 2012-05-02 2021-06-15 Ibis Biosciences Inc DNA sequencing
ES2683707T3 (en) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. DNA sequencing
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
EP3438286B1 (en) 2012-05-02 2020-10-28 Ibis Biosciences, Inc. Dna sequencing
EP2850086B1 (en) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
WO2014008448A1 (en) 2012-07-03 2014-01-09 Sloan Kettering Institute For Cancer Research Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation
AU2013292287A1 (en) 2012-07-19 2015-02-19 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
CA3040684C (en) 2012-08-20 2023-02-07 Hod Finkelstein Method and system for fluorescence lifetime based sequencing
ES2701750T3 (en) 2012-10-16 2019-02-25 Abbott Molecular Inc Procedures for sequencing a nucleic acid
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014113557A1 (en) 2013-01-18 2014-07-24 Nabsys, Inc. Enhanced probe binding
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US20160023208A1 (en) 2013-03-13 2016-01-28 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
EP3553175B1 (en) 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
JP2016512437A (en) 2013-03-14 2016-04-28 アボツト・モレキユラー・インコーポレイテツド Multiple methylation specific amplification systems and methods
ES2674043T5 (en) 2013-03-14 2021-11-03 Illumina Inc Modified polymerases for improved incorporation of nucleotide analogs
EP2971154A4 (en) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nucleic acid control panels
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
EP2972281B1 (en) 2013-03-15 2023-07-26 Life Technologies Corporation Chemical device with thin conductive element
CN105358709B (en) 2013-03-15 2018-12-07 雅培分子公司 System and method for detecting genome copy numbers variation
US20160153029A1 (en) 2013-03-15 2016-06-02 Ibis Biosciences, Inc. Dna sequences to assess contamination in dna sequencing
US20140264472A1 (en) 2013-03-15 2014-09-18 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
KR102230831B1 (en) 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 Modified nucleosides or nucleotides
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
EP3004385B1 (en) 2013-05-24 2018-11-28 Quantapore Inc. Nanopore-based nucleic acid analysis with mixed fret detection
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
DK3013983T3 (en) 2013-06-25 2023-03-06 Prognosys Biosciences Inc SPATIALLY ENCODED BIOLOGICAL ASSAYS USING A MICROFLUIDIC DEVICE
MX363806B (en) 2013-07-01 2019-04-03 Illumina Inc Catalyst-free surface functionalization and polymer grafting.
EP3241913B1 (en) 2013-07-03 2019-02-20 Illumina, Inc. System for sequencing by orthogonal synthesis
CN103333680B (en) * 2013-07-16 2015-05-27 北京化工大学 Dinyl oxazole eutectic material with multi-color fluorescence characteristic and preparation method of dinyl oxazole eutectic material
FI3030645T3 (en) 2013-08-08 2023-02-10 Fluidic system for reagent delivery to a flow cell
US20150051088A1 (en) 2013-08-19 2015-02-19 Abbott Molecular Inc. Next-generation sequencing libraries
US9567645B2 (en) 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
JP2016539343A (en) 2013-08-30 2016-12-15 イルミナ インコーポレイテッド Manipulating droplets on hydrophilic or mottled hydrophilic surfaces
US10351899B2 (en) 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
EP3052652B1 (en) 2013-09-30 2022-11-23 Life Technologies Corporation Polymerase compositions, methods of making and using same
JP2017504307A (en) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド Method and system for digitally counting features on an array
AU2014334531B2 (en) 2013-10-18 2019-05-16 The Regents Of The University Of Michigan Systems and methods for determining a treatment course of action
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
PL3077943T3 (en) 2013-12-03 2020-11-30 Illumina, Inc. Methods and systems for analyzing image data
CN110411998B (en) 2013-12-10 2022-06-07 伊鲁米那股份有限公司 Biosensors for biological or chemical analysis and methods for their manufacture
EP3083700B1 (en) 2013-12-17 2023-10-11 The Brigham and Women's Hospital, Inc. Detection of an antibody against a pathogen
US10246746B2 (en) 2013-12-20 2019-04-02 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
KR102333635B1 (en) 2013-12-23 2021-11-30 일루미나, 인코포레이티드 Structured substrates for improving detection of light emissions and methods relating to the same
JP6666852B2 (en) * 2014-01-16 2020-03-18 イルミナ インコーポレイテッド Gene expression panel for prognosis of prostate cancer recurrence
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015108663A1 (en) 2014-01-16 2015-07-23 Illumina, Inc. Amplicon preparation and sequencing on solid supports
JP7011392B2 (en) 2014-02-18 2022-01-26 イラミーナ インコーポレーテッド Methods and compositions for DNA profiling
CN110982689A (en) 2014-03-11 2020-04-10 伊鲁米那股份有限公司 Disposable integrated microfluidic cartridge and methods of making and using same
CA3060708A1 (en) 2014-04-29 2015-11-05 Illumina, Inc Multiplexed single cell gene expression analysis using template switch and tagmentation
ES2876432T3 (en) 2014-05-16 2021-11-12 Illumina Inc Nucleic acid synthesis techniques
CA2949984C (en) 2014-05-27 2021-10-19 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
CN106687574B (en) 2014-06-03 2021-06-29 亿明达股份有限公司 Compositions, systems and methods for detecting events using tethers anchored to or near nanoparticles
US10760109B2 (en) 2014-06-06 2020-09-01 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US10829814B2 (en) 2014-06-19 2020-11-10 Illumina, Inc. Methods and compositions for single cell genomics
EP3919621A1 (en) 2014-06-23 2021-12-08 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
WO2015200541A1 (en) 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2015200833A2 (en) 2014-06-27 2015-12-30 Abbott Laboratories COMPOSITIONS AND METHODS FOR DETECTING HUMAN PEGIVIRUS 2 (HPgV-2)
EP3161154B1 (en) 2014-06-27 2020-04-15 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
ES2697428T3 (en) 2014-07-15 2019-01-23 Illumina Inc Electronic device activated biochemically
CN112941065A (en) 2014-07-21 2021-06-11 亿明达股份有限公司 Polynucleotide enrichment Using CRISPR-CAS System
RU2697502C2 (en) 2014-07-24 2019-08-14 Эбботт Молекьюлар Инк. Compositions and methods for detecting and analyzing micobacterium tuberculosis
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP3183577B1 (en) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible surface functionalization
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
WO2016040607A1 (en) 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
JP6802154B2 (en) 2014-09-18 2020-12-16 イラミーナ インコーポレーテッド Methods and systems for analyzing nucleic acid sequencing data
US9677057B2 (en) 2014-09-30 2017-06-13 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
WO2016153999A1 (en) 2015-03-25 2016-09-29 Life Technologies Corporation Modified nucleotides and uses thereof
KR20170066540A (en) 2014-10-09 2017-06-14 일루미나, 인코포레이티드 Method and device for separating immiscible liquids to effectively isolate at least one of the liquids
ES2789000T3 (en) 2014-10-10 2020-10-23 Quantapore Inc Nanopore-based polynucleotide analysis with mutually inactivating fluorescent labels
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
JP6808617B2 (en) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド Dislocations that maintain continuity
CA2964790C (en) 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
CN114805710B (en) 2014-10-31 2023-11-17 伊鲁米纳剑桥有限公司 Polymer and DNA copolymer coating
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
ES2768762T3 (en) 2014-11-05 2020-06-23 Illumina Cambridge Ltd Reduction of DNA damage during sample preparation and sequencing using siderophore chelating agents
CN107109495B (en) 2014-11-11 2021-12-24 伊鲁米那股份有限公司 Polynucleotide amplification using CRISPR-CAS system
WO2016075204A1 (en) 2014-11-11 2016-05-19 Illumina, Inc. Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
RU2582198C1 (en) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Analogues of natural deoxyribonucleoside triphosphates and ribonucleoside triphosphates containing reporter fluorescent groups, for use in analytical bioorganic chemistry
CN104458686B (en) * 2014-12-02 2017-01-18 公安部第一研究所 DNA fluorescence spectrum collecting method based on characteristic molecular weight interior label quantitative analysis
EP3234187B1 (en) 2014-12-15 2021-02-17 Illumina, Inc. Method for single molecular placement on a substrate
EP3795681B1 (en) 2014-12-16 2024-09-18 Life Technologies Corporation Polymerase compositions and methods of making and using same
EP3234576B1 (en) 2014-12-18 2023-11-22 Life Technologies Corporation High data rate integrated circuit with transmitter configuration
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
US10379079B2 (en) 2014-12-18 2019-08-13 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
BR112017017025B1 (en) 2015-02-10 2024-01-02 Illumina, Inc METHODS AND COMPOSITIONS FOR ANALYZING CELLULAR COMPONENTS
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016134342A1 (en) 2015-02-20 2016-08-25 Wafergen, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
CN107208158B (en) 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
US10576471B2 (en) 2015-03-20 2020-03-03 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
US9976174B2 (en) 2015-03-24 2018-05-22 Illumina Cambridge Limited Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
EP3835431B1 (en) 2015-03-30 2022-11-02 Becton, Dickinson and Company Methods for combinatorial barcoding
US20180100192A1 (en) 2015-03-31 2018-04-12 lllumina Cambridge Limited Surface concatemerization of templates
CN120425026A (en) 2015-04-10 2025-08-05 十程基因技术瑞典公司 Spatial differentiation of biological samples and multiplex nucleic acid analysis
CN108449971B (en) 2015-04-14 2020-10-16 亿明达股份有限公司 Structured substrate for improved detection of light emission and method relating thereto
AU2016250281A1 (en) 2015-04-15 2017-10-26 The General Hospital Corporation LNA-based mutant enrichment next-generation sequencing assays
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
JP2018518157A (en) 2015-05-11 2018-07-12 イラミーナ インコーポレーテッド Platform for therapeutic drug discovery and analysis
JP6609641B2 (en) 2015-05-29 2019-11-20 イルミナ ケンブリッジ リミテッド Advanced use of surface primers in clusters
KR102134953B1 (en) 2015-05-29 2020-07-16 일루미나, 인코포레이티드 Sample carrier and assay system for carrying out the specified reaction
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
IL293410B2 (en) 2015-06-03 2024-01-01 Illumina Inc Compositions, systems and methods for sequencing polynucleotides using tethers anchored to polymerases attached to nanopores
CN107922966B (en) 2015-07-06 2022-06-28 伊卢米纳剑桥有限公司 Sample preparation for nucleic acid amplification
GB2556713B (en) 2015-07-06 2021-06-23 Illumina Inc Balanced AC modulation for driving droplet operations electrodes
CN107924121B (en) 2015-07-07 2021-06-08 亿明达股份有限公司 Selective Surface Patterning via Nanoimprinting
EP3322483A4 (en) 2015-07-14 2019-01-02 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
CA2992449A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
WO2017015018A1 (en) 2015-07-17 2017-01-26 Illumina, Inc. Polymer sheets for sequencing applications
WO2017014762A1 (en) * 2015-07-21 2017-01-26 Omniome, Inc. Nucleic acid sequencing methods and systems
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
WO2017019456A2 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
AU2016298541B2 (en) 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
ES2868195T3 (en) 2015-08-14 2021-10-21 Illumina Inc Systems and methods that use magnetically sensitive sensors to determine a genetic trait
CN108474805A (en) 2015-08-24 2018-08-31 亿明达股份有限公司 In-line accumulators and flow control systems for biological and chemical assays
AU2016316773B2 (en) 2015-08-28 2020-01-30 Illumina, Inc. Nucleic acid sequence analysis from single cells
CN107921432A (en) 2015-09-02 2018-04-17 伊卢米纳剑桥有限公司 Improve the system and method for the droplet manipulation in flow control system
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド Methods and compositions for library normalization
CN108350453A (en) 2015-09-11 2018-07-31 通用医疗公司 The rough complete querying of nuclease DSB and sequencing (FIND-SEQ)
JP2018529353A (en) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション Comprehensive in vitro reporting of cleavage events by sequencing (CIRCLE-seq)
US10344268B2 (en) 2015-10-01 2019-07-09 Life Technologies Corporation Polymerase compositions and kits, and methods of using and making the same
SG11201803293SA (en) 2015-10-22 2018-05-30 Illumina Inc Filler fluid for fluidic devices
US11203016B2 (en) 2015-12-01 2021-12-21 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
CN109072300B (en) 2015-12-17 2023-01-31 伊路敏纳公司 Differentiating methylation levels in complex biological samples
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
EP3848457B1 (en) 2016-02-08 2025-08-13 Rgene, Inc. Multiple ligase compositions, systems, and methods
CN109477127A (en) 2016-03-14 2019-03-15 R基因股份有限公司 Hyperthermostable lysine-mutant ssDNA/RNA ligase
SG11201805787YA (en) 2016-03-24 2018-10-30 Illumina Inc Photonic superlattice-based devices and compositions for use in luminescent imaging, and methods of using the same
PL3377226T3 (en) 2016-03-28 2021-07-26 Illumina, Inc. Multi-plane microarrays
WO2017177017A1 (en) 2016-04-07 2017-10-12 Omniome, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
WO2017176896A1 (en) 2016-04-07 2017-10-12 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
KR20180099903A (en) 2016-04-22 2018-09-05 옴니옴 인코포레이티드 Nucleic acid sequence determination methods and systems using improved detection of nucleotide-specific tertiary complex formation
WO2017184997A1 (en) 2016-04-22 2017-10-26 Illumina, Inc. Photonic stucture-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same
CA3021769C (en) 2016-04-29 2021-11-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
EP3452614B1 (en) 2016-05-02 2023-06-28 Becton, Dickinson and Company Accurate molecular barcoding
EP3455372B1 (en) 2016-05-11 2019-10-30 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
SG11201809376WA (en) 2016-05-18 2018-12-28 Illumina Inc Self assembled patterning using patterned hydrophobic surfaces
CA3025031A1 (en) * 2016-05-20 2017-11-23 Quantum-Si Incorporated Labeled nucleotide compositions and methods for nucleic acid sequencing
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109074430B (en) 2016-05-26 2022-03-29 贝克顿迪金森公司 Molecular marker counting adjustment method
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10823721B2 (en) 2016-07-05 2020-11-03 Quantapore, Inc. Optically based nanopore sequencing
WO2018013509A1 (en) 2016-07-11 2018-01-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
EP3875603A1 (en) 2016-07-12 2021-09-08 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices
KR102475710B1 (en) 2016-07-22 2022-12-08 오레곤 헬스 앤드 사이언스 유니버시티 Single-cell whole-genome libraries and combinatorial indexing methods for their preparation
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
WO2018042251A1 (en) 2016-08-29 2018-03-08 Oslo Universitetssykehus Hf Chip-seq assays
AU2017331459B2 (en) 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
EP4439045A3 (en) 2016-10-14 2024-11-27 Illumina, Inc. Cartridge assembly
ES2927350T3 (en) 2016-10-19 2022-11-04 Illumina Inc Methods for chemical ligation of nucleic acids
AU2017359048B2 (en) 2016-11-08 2022-07-21 Becton, Dickinson And Company Methods for expression profile classification
KR102790039B1 (en) 2016-11-08 2025-04-04 벡톤 디킨슨 앤드 컴퍼니 Cell marker classification method
WO2018093780A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
BR112018015871B1 (en) 2016-12-09 2021-12-07 The Broad Institute, Inc. SYSTEM, METHOD AND DEVICE TO DETECT THE PRESENCE OF A TARGET NUCLEIC ACID SEQUENCE IN A SAMPLE
WO2018118971A1 (en) 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
CA3046532A1 (en) 2016-12-22 2018-06-28 Illumina, Inc. Arrays including a resin film and a patterned polymer layer
CN110325651B (en) 2016-12-22 2024-03-15 伊鲁米那股份有限公司 Arrays with quality control tracers
RU2765075C2 (en) 2016-12-22 2022-01-25 Иллюмина, Инк. Biochip including sequencing primer and non-sequencing unit
WO2018121587A1 (en) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 Single fluorescent dye based sequencing method
CA3048415C (en) 2016-12-30 2023-02-28 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
MX2019008055A (en) 2017-01-06 2019-11-05 Illumina Inc Phasing correction.
CA3049722C (en) 2017-01-10 2023-01-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent dna binding
ES2961580T3 (en) 2017-01-13 2024-03-12 Cellular Res Inc Hydrophilic coating of fluid channels
CN110178184B (en) 2017-01-17 2024-04-19 Illumina公司 Oncogenic splice variant determination
US10844429B2 (en) 2017-01-18 2020-11-24 Illumina, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
CA3050241C (en) 2017-01-20 2022-11-29 Omniome, Inc. Allele-specific capture of nucleic acids
EP3565900B1 (en) 2017-01-20 2021-04-07 Omniome, Inc. Genotyping by polymerase binding
US10975427B2 (en) 2017-01-20 2021-04-13 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
DK3354746T3 (en) 2017-01-30 2019-09-02 Gmi Gregor Mendel Inst Fuer Molekulare Pflanzenbiologie Gmbh NEW SPIKE-IN NUCLEOTIDES FOR NORMALIZING SEQUENCE DATA
US11896944B2 (en) 2017-02-01 2024-02-13 Illumina, Inc. System and method with fiducials responding to multiple excitation frequencies
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification using blocking oligonucleotides
US11492666B2 (en) 2017-02-15 2022-11-08 Pacific Biosciences Of California, Inc. Distinguishing sequences by detecting polymerase dissociation
FI3452621T3 (en) 2017-02-21 2022-12-15 TAGMENTATION WITH IMMOBILIZED TRANSPOSOME WITH LINKS
EP3592768B1 (en) 2017-03-08 2024-12-25 The Regents of The University of Michigan Analyte detection
JP2020513815A (en) 2017-03-15 2020-05-21 ザ・ブロード・インスティテュート・インコーポレイテッド Diagnostic method for virus detection based on clustered short repeat palindrome effector system
US11021740B2 (en) 2017-03-15 2021-06-01 The Broad Institute, Inc. Devices for CRISPR effector system based diagnostics
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
CN116926061A (en) 2017-03-20 2023-10-24 Illumina公司 Methods and compositions for preparing nucleic acid libraries
EP3601593B1 (en) 2017-03-24 2021-12-22 Bio-Rad Laboratories, Inc. Universal hairpin primers
US10737267B2 (en) 2017-04-04 2020-08-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
ES2893528T3 (en) 2017-04-23 2022-02-09 Illumina Cambridge Ltd Compositions and methods to improve sample identification in indexed nucleic acid collections
ES2937929T3 (en) 2017-04-23 2023-04-03 Illumina Inc Compositions and methods to improve sample identification in indexed nucleic acid libraries
AU2018259202B2 (en) 2017-04-23 2022-03-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
CN110832087B (en) 2017-05-08 2025-03-18 伊鲁米那股份有限公司 Universal short adapter for indexing of polynucleotide samples
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
KR102628035B1 (en) 2017-06-07 2024-01-22 오레곤 헬스 앤드 사이언스 유니버시티 Single cell whole genome library for methylation sequencing
EP3634992B1 (en) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
US11702654B2 (en) 2017-06-20 2023-07-18 Illumina, Inc. Methods and compositions for addressing inefficiencies in amplification reactions
WO2019002265A1 (en) 2017-06-26 2019-01-03 Universität Für Bodenkultur Wien Novel biomarkers for detecting senescent cells
US11578180B2 (en) 2017-07-18 2023-02-14 Pacific Biosciences Of California, Inc. Method of chemically modifying plastic surfaces
KR102626317B1 (en) 2017-07-24 2024-01-18 퀀텀-에스아이 인코포레이티드 High-Intensity Labeled Reactant Compositions and Sequencing Methods
EP3662482A1 (en) 2017-07-31 2020-06-10 Illumina Inc. Sequencing system with multiplexed biological sample aggregation
FI3662083T3 (en) 2017-08-01 2024-11-20 Illumina Inc Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
KR102480894B1 (en) 2017-08-01 2022-12-23 일루미나, 인코포레이티드 Hydrogel beads for nucleotide sequencing
JP6998404B2 (en) 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 Method for enriching and determining the target nucleotide sequence
SG11202000964UA (en) 2017-08-15 2020-02-27 Omniome Inc Scanning apparatus and methods useful for detection of chemical and biological analytes
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
WO2019060640A1 (en) 2017-09-20 2019-03-28 Guardant Health, Inc. Methods and systems for differentiating somatic and germline variants
NZ759818A (en) 2017-10-16 2022-04-29 Illumina Inc Semi-supervised learning for training an ensemble of deep convolutional neural networks
KR20220031940A (en) 2017-10-16 2022-03-14 일루미나, 인코포레이티드 Aberrant splicing detection using convolutional neural networks (cnns)
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US10907205B2 (en) 2017-11-02 2021-02-02 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides
IL326759A (en) 2017-12-29 2026-04-01 Nautilus Subsidiary Inc Decoding approaches for protein identification
CA3279137A1 (en) 2018-01-08 2025-10-30 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
EP3619653B1 (en) 2018-01-15 2021-05-19 Illumina Inc. Deep learning-based variant classifier
US20210371926A1 (en) 2018-01-29 2021-12-02 The Broad Institute, Inc. Crispr effector system based diagnostics
CN119614672A (en) 2018-01-31 2025-03-14 生物辐射实验室股份有限公司 Methods and compositions for deconvoluting partitioned barcodes
KR20200139671A (en) 2018-02-06 2020-12-14 옴니옴 인코포레이티드 Composition and technology for nucleic acid primer extension
EP4253562A3 (en) 2018-02-13 2023-10-25 Illumina, Inc. Dna sequencing using hydrogel beads
CN110832086A (en) 2018-04-02 2020-02-21 伊鲁米那股份有限公司 Compositions and methods for making controls for sequence-based genetic testing
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
NL2020861B1 (en) 2018-04-12 2019-10-22 Illumina Inc Variant classifier based on deep neural networks
CN112424377A (en) 2018-04-17 2021-02-26 克罗玛科德公司 Method and system for multiplex analysis
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
AU2019257320B2 (en) 2018-04-20 2022-04-07 Illumina, Inc. Methods of encapsulating single cells, the encapsulated cells and uses thereof
WO2019209426A1 (en) 2018-04-26 2019-10-31 Omniome, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
US12235273B2 (en) 2018-05-04 2025-02-25 Abbott Laboratories HBV diagnostic, prognostic, and therapeutic methods and products
US12084474B2 (en) 2018-05-15 2024-09-10 Illumina, Inc. Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides
KR102640255B1 (en) 2018-05-17 2024-02-27 일루미나, 인코포레이티드 High-throughput single-cell sequencing with reduced amplification bias
US12352745B2 (en) 2018-05-25 2025-07-08 Illumina, Inc. Circulating RNA signatures specific to preeclampsia
AU2019276719B2 (en) 2018-05-31 2025-11-13 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
JP2021526791A (en) 2018-06-04 2021-10-11 ガーダント ヘルス， インコーポレイテッド Methods and systems for determining the cellular origin of cell-free nucleic acids
KR102507415B1 (en) 2018-06-04 2023-03-07 일루미나, 인코포레이티드 High-throughput single-cell transcriptome libraries and methods of making and of using
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
EP3820943A1 (en) 2018-07-13 2021-05-19 Quantum-si Incorporated Biconjugatable labels and methods of use
AU2019310041B2 (en) 2018-07-23 2025-11-20 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
WO2020023362A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
WO2020033601A1 (en) 2018-08-07 2020-02-13 The Broad Institute, Inc. Novel cas12b enzymes and systems
CA3109238A1 (en) 2018-08-15 2020-02-20 Illumina, Inc. Compositions and methods for improving library enrichment
CN113166807B (en) 2018-08-20 2024-06-04 生物辐射实验室股份有限公司 Nucleotide sequence generation by co-localization of barcode beads in partitions
CN109161536B (en) * 2018-08-20 2022-04-08 天津科技大学 Enzyme preparation for preparing uridylic acid and method for preparing uridylic acid by enzyme catalysis
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CA3109646A1 (en) 2018-08-30 2020-03-05 Guardant Health, Inc. Methods and systems for detecting contamination between samples
SG11202101400UA (en) 2018-08-31 2021-03-30 Guardant Health Inc Microsatellite instability detection in cell-free dna
JP7637615B2 (en) 2018-09-04 2025-02-28 ガーダント ヘルス， インコーポレイテッド Methods and systems for detecting allelic imbalance in cell-free nucleic acid samples - Patents.com
WO2020060811A1 (en) 2018-09-17 2020-03-26 Omniome, Inc. Engineered polymerases for improved sequencing
CA3110668C (en) 2018-09-20 2022-01-11 Tamirna Gmbh Micro-rna signatures for the prediction of liver dysfunction
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
US12467925B2 (en) 2018-10-03 2025-11-11 The Broad Institute, Inc. CRISPR effector system based diagnostics for hemorrhagic fever detection
JP6888123B2 (en) 2018-10-15 2021-06-16 イルミナ インコーポレイテッド Deep learning-based technology for pre-training deep convolutional neural networks
CA3113271A1 (en) 2018-10-26 2020-04-30 Illumina, Inc. Modulating polymer beads for dna processing
EP3874060B1 (en) 2018-10-31 2024-10-16 Guardant Health, Inc. Method and system for calibrating epigenetic partitioning assays
CA3103719A1 (en) 2018-10-31 2020-05-07 Illumina, Inc. Polymerases, compositions, and methods of use
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
US12139764B2 (en) 2018-11-14 2024-11-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating breast cancer
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
MX2021003771A (en) 2018-11-30 2021-05-27 Illumina Inc Analysis of multiple analytes using a single assay.
CN211311438U (en) 2018-12-04 2020-08-21 欧姆尼欧美公司 Flow cell
CA3103739A1 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
EP4745247A2 (en) 2018-12-13 2026-05-20 Becton Dickinson Co Selective extension in single-cell full transcripto analysis
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
WO2020120179A1 (en) 2018-12-14 2020-06-18 Illumina Cambridge Limited Decreasing phasing with unlabeled nucleotides during sequencing
WO2020126593A1 (en) 2018-12-17 2020-06-25 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
JP7562424B2 (en) 2018-12-17 2024-10-07 イルミナ ケンブリッジ リミテッド Sequencing primer oligonucleotides
JP7587425B2 (en) 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド Methods and compositions for paired-end sequencing using a single surface primer
BR112020026667A2 (en) 2018-12-19 2021-07-27 Illumina, Inc. methods to improve the clonality priority of the polynucleotide cluster
JP2022514010A (en) 2018-12-20 2022-02-09 ガーダント ヘルス， インコーポレイテッド Methods, compositions, and systems for improving the recovery of nucleic acid molecules
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
BR112021006038A2 (en) 2019-01-11 2021-10-26 Illumina Cambridge Limited STRAPOSOME COMPLEXES CONNECTED TO THE SURFACE OF THE COMPLEX
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
CN113574178B (en) 2019-01-23 2024-10-29 贝克顿迪金森公司 Oligonucleotides linked to antibodies
CN113614097A (en) 2019-01-23 2021-11-05 宽腾矽公司 High-strength labeled reactant compositions and sequencing methods
CA3126428A1 (en) 2019-01-31 2020-08-06 Guardant Health, Inc. Compositions and methods for isolating cell-free dna
CN120099139A (en) 2019-02-14 2025-06-06 贝克顿迪金森公司 Heterozygote targeted and whole transcriptome amplification
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (en) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 Scanning device and method for detecting chemical and biological analytes
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
US12624350B2 (en) 2019-03-01 2026-05-12 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11939636B2 (en) 2019-05-31 2024-03-26 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
AU2020312787A1 (en) 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
SG11202105836XA (en) 2019-07-12 2021-07-29 Illumina Cambridge Ltd Compositions and methods for preparing nucleic acid sequencing libraries using crispr/cas9 immobilized on a solid support
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
CN120099137A (en) 2019-07-22 2025-06-06 贝克顿迪金森公司 Single-cell chromatin immunoprecipitation sequencing assay
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
WO2021041519A1 (en) 2019-08-26 2021-03-04 Pact Pharma, Inc. Methods of performing guide-seq on primary human t cells
TW202124406A (en) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 Reversible modification of nucleotides
US20220290245A1 (en) 2019-09-11 2022-09-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Servic Cancer detection and classification
EP4715839A2 (en) 2019-10-11 2026-03-25 Guardant Health, Inc. Use of cell free bacterial nucleic acids for detection of cancer
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
LT3812472T (en) 2019-10-21 2023-03-10 Albert-Ludwigs-Universität Freiburg A truly unbiased in vitro assay to profile off-target activity of one or more target-specific programmable nucleases in cells (abnoba-seq)
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4055155A1 (en) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Engineered polymerases for improved sequencing by binding
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN113677807A (en) 2019-11-22 2021-11-19 因美纳有限公司 Preeclampsia-specific circulating RNA markers
CN114746560A (en) 2019-11-26 2022-07-12 夸登特健康公司 Methods, compositions, and systems for improved binding of methylated polynucleotides
DE202019106694U1 (en) 2019-12-02 2020-03-19 Omniome, Inc. System for sequencing nucleic acids in fluid foam
DE202019106695U1 (en) 2019-12-02 2020-03-19 Omniome, Inc. System for sequencing nucleic acids in fluid foam
CA3131632A1 (en) 2019-12-04 2021-06-10 Tong Liu Preparation of dna sequencing libraries for detection of dna pathogens in plasma
MX2021011847A (en) 2019-12-19 2021-11-17 Illumina Inc High-throughput single-cell libraries and methods of making and of using.
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN121022991A (en) 2019-12-23 2025-11-28 10X基因组学有限公司 Spatial analysis using RNA templated ligation
EP4081777B1 (en) 2019-12-23 2026-02-18 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
EP4090763B1 (en) 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
WO2021158511A1 (en) 2020-02-04 2021-08-12 Omniome, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN111311265B (en) * 2020-02-13 2023-07-25 布比（北京）网络技术有限公司 Blockchain private transaction proving method, blockchain private transaction proving device, computer equipment and storage medium
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12592298B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Hardware execution and acceleration of artificial intelligence-based base caller
US12591780B2 (en) 2020-02-20 2026-03-31 Illumina, Inc. Data compression for artificial intelligence-based base calling
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
KR20220143854A (en) 2020-02-20 2022-10-25 일루미나, 인코포레이티드 AI-based many-to-many base calling
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115151810A (en) 2020-02-25 2022-10-04 贝克顿迪金森公司 Dual specificity probes enabling the use of single cell samples as monochromatic compensation controls
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4127220B1 (en) 2020-03-30 2024-06-05 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
CA3177127A1 (en) 2020-04-30 2021-11-04 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
CN115836135A (en) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 Compositions and methods for modifying polymerase-nucleic acid complexes
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4150065A2 (en) 2020-05-12 2023-03-22 Illumina Inc Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
US12241126B2 (en) 2020-05-14 2025-03-04 Guardant Health, Inc. Homologous recombination repair deficiency detection
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
CN116134308B (en) 2020-05-19 2025-05-27 10X基因组学有限公司 Electrophoresis cartridge and instrument
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158054B1 (en) 2020-06-02 2025-04-16 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
WO2021247543A2 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
CN115803445A (en) 2020-06-02 2023-03-14 贝克顿迪金森公司 Oligonucleotides and beads for 5-prime gene expression assays
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US20230193356A1 (en) 2020-06-08 2023-06-22 The Broad Institute, Inc. Single cell combinatorial indexing from amplified nucleic acids
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
AU2021288692A1 (en) 2020-06-11 2023-02-02 Nautilus Subsidiary, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
WO2021259881A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
US20230242967A1 (en) 2020-07-02 2023-08-03 Illumina, Inc. A method to calibrate nucleic acid library seeding efficiency in flowcells
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
BR112023000073A2 (en) 2020-07-08 2023-04-11 Illumina Inc MICROSPHERES AS TRANSPOSOME TRANSPORTERS
EP4179111B1 (en) 2020-07-10 2024-04-03 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12116637B2 (en) 2020-07-24 2024-10-15 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
WO2022026761A1 (en) 2020-07-30 2022-02-03 Guardant Health, Inc. Methods for isolating cell-free dna
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CA3190588A1 (en) 2020-08-06 2022-02-10 Illumina, Inc. Preparation of rna and dna sequencing libraries using bead-linked transposomes
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
IL299783A (en) 2020-08-18 2023-03-01 Illumina Inc Sequence-specific targeted transposition and selection and sorting of nucleic acids
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022046947A1 (en) 2020-08-25 2022-03-03 Guardant Health, Inc. Methods and systems for predicting an origin of a variant
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
IL300983A (en) 2020-09-11 2023-04-01 Illumina Cambridge Ltd Methods of enriching a target sequence from a sequencing library using hairpin adaptors
WO2022061150A2 (en) 2020-09-18 2022-03-24 10X Geonomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022073012A1 (en) 2020-09-30 2022-04-07 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and a methylation-dependent nuclease
AU2021366658A1 (en) 2020-10-21 2023-06-22 Illumina Cambridge Limited Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
WO2022087309A1 (en) 2020-10-23 2022-04-28 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and base conversion
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
EP4121555B1 (en) 2020-12-21 2026-02-25 10X Genomics, Inc. Methods for capturing probes and/or barcodes
WO2022140629A1 (en) 2020-12-23 2022-06-30 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
WO2022155331A1 (en) 2021-01-13 2022-07-21 Pacific Biosciences Of California, Inc. Surface structuring with colloidal assembly
WO2022165276A1 (en) 2021-01-29 2022-08-04 Armonica Technologies, Inc. Enhancement structures for surface-enhanced raman scattering
KR20230154303A (en) 2021-01-29 2023-11-07 일루미나, 인코포레이티드 Methods, compositions and kits for improving seeding efficiency of flow cells with polynucleotides
ES3015114T3 (en) 2021-02-04 2025-04-29 Illumina Inc Long indexed-linked read generation on transposome bound beads
WO2022174054A1 (en) 2021-02-13 2022-08-18 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
EP4421491A3 (en) 2021-02-19 2024-11-27 10X Genomics, Inc. Method of using a modular assay support device
JP2024513668A (en) 2021-03-05 2024-03-27 ガーダント ヘルス， インコーポレイテッド Methods and related aspects for analyzing molecular responses
WO2022192889A1 (en) 2021-03-09 2022-09-15 Guardant Health, Inc. Detecting the presence of a tumor based on off-target polynucleotide sequencing data
US12525320B2 (en) 2021-03-16 2026-01-13 Illumina, Inc. Neural network parameter quantization for base calling
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
AU2022238446A1 (en) 2021-03-18 2023-09-07 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
CA3210451A1 (en) 2021-03-22 2022-09-29 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
JP2024511766A (en) 2021-03-29 2024-03-15 イルミナ インコーポレイテッド Improved library preparation method
CA3214282A1 (en) 2021-03-29 2022-10-06 Illumina, Inc. Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias
MX2023010495A (en) 2021-03-30 2023-09-18 Illumina Inc Improved methods of isothermal complementary dna and library preparation.
KR20230164668A (en) 2021-03-31 2023-12-04 일루미나, 인코포레이티드 Method for manufacturing directed tagged sequencing libraries using transposon-based technology using unique molecular identifiers for error correction
WO2022212180A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Artificial intelligence-based base caller with contextual awareness
JP7719206B2 (en) 2021-04-02 2025-08-05 イルミナ インコーポレイテッド Nucleotide for sequencing - Machine learning model for detecting bubbles in specimen slides
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12444482B2 (en) 2021-04-15 2025-10-14 Illumina, Inc. Multi-channel protein voxelization to predict variant pathogenicity using deep convolutional neural networks
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US12070744B2 (en) 2021-04-22 2024-08-27 Illumina, Inc. Valve assemblies and related systems
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4337789A1 (en) 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
EP4337786A1 (en) 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Single-molecule seeding and amplification on a surface
CN117916390A (en) 2021-05-20 2024-04-19 伊鲁米纳公司 Compositions and methods for sequencing-by-synthesis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12570831B2 (en) 2021-06-15 2026-03-10 Illumina, Inc. Hydrogel-free surface functionalization for sequencing
CN117597453A (en) 2021-06-23 2024-02-23 伊鲁米纳公司 Compositions, methods, kits, cartridges and systems for sequencing reagents
EP4359557B1 (en) 2021-06-24 2026-05-20 Illumina Inc METHOD AND COMPOSITIONS FOR THE COMBINATORY INDEXATION OF NUCLEAN ACIDS ON A SPOON BASE
KR20240022490A (en) 2021-06-29 2024-02-20 일루미나, 인코포레이티드 Signal-to-noise ratio metrics for determining nucleotide base calling and base calling quality
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
AU2022300970A1 (en) 2021-06-29 2024-01-18 Illumina, Inc. Self-learned base caller, trained using oligo sequences
EP4364149A1 (en) 2021-06-29 2024-05-08 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
US12530882B2 (en) 2021-07-01 2026-01-20 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US12423815B2 (en) 2021-07-13 2025-09-23 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
WO2023004357A1 (en) 2021-07-23 2023-01-26 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
CA3223746A1 (en) 2021-07-28 2023-02-02 Rohan PAUL Quality score calibration of basecalling systems
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
KR20240035413A (en) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 Base calling using the multi-base caller model
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CA3223390A1 (en) 2021-08-17 2023-02-23 Colin Brown Methods and compositions for identifying methylated cytosines
CN118103750A (en) 2021-08-31 2024-05-28 伊鲁米纳公司 Flow cell with enhanced pore imaging resolution
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
JP2024535663A (en) 2021-09-17 2024-10-02 イルミナ インコーポレイテッド Automatic Identification of Sources of Faults from Base Call Error Patterns in Nucleotide Sequencing
US20230095961A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. Graph reference genome and base-calling approach using imputed haplotypes
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
WO2023056328A2 (en) 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
US12480157B2 (en) 2021-09-30 2025-11-25 Illumina, Inc. Polynucleotide sequencing
US20230114905A1 (en) 2021-10-11 2023-04-13 Nautilus Biotechnology, Inc. Highly multiplexable analysis of proteins and proteomes
CA3234961A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441743A1 (en) 2021-12-02 2024-10-09 Illumina, Inc. Generating cluster-specific-signal corrections for determining nucleotide-base calls
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
US12403467B2 (en) 2021-12-23 2025-09-02 Illumina, Inc. Systems and related temperature calibration methods
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
EP4457814A1 (en) 2021-12-29 2024-11-06 Illumina, Inc. Automatically switching variant analysis model versions for genomic analysis applications
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
EP4483371A1 (en) 2022-02-25 2025-01-01 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
US20230410944A1 (en) 2022-02-25 2023-12-21 Illumina, Inc. Calibration sequences for nucelotide sequencing
US20230343414A1 (en) 2022-03-25 2023-10-26 Illumina, Inc. Sequence-to-sequence base calling
US12092578B2 (en) 2022-03-29 2024-09-17 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
EP4504930A1 (en) 2022-04-07 2025-02-12 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
EP4523213A1 (en) 2022-05-09 2025-03-19 Guardant Health, Inc. Detecting degradation based on strand bias
EP4523215A1 (en) 2022-05-10 2025-03-19 Illumina Inc. Adaptive neural network for nucelotide sequencing
WO2023235353A2 (en) 2022-06-03 2023-12-07 Illumina, Inc. Circulating rna biomarkers for preeclampsia
JP2025526537A (en) 2022-06-09 2025-08-15 イルミナ インコーポレイテッド Dependence of base calling on flow cell tilt
WO2023240494A1 (en) * 2022-06-15 2023-12-21 深圳华大智造科技股份有限公司 Sequencing buffer and method for improving stability of dntp modified with reversible blocking group
KR20250034034A (en) 2022-06-24 2025-03-10 일루미나, 인코포레이티드 How to improve split-read alignment by intelligently identifying and scoring candidate split groups
CA3260497A1 (en) 2022-06-27 2024-01-04 Illumina, Inc. Accelerators for a genotype imputation model
EP4544554A1 (en) 2022-06-27 2025-04-30 Illumina, Inc. Improved human leukocyte antigen (hla) genotyping
IL317959A (en) 2022-06-27 2025-02-01 Illumina Inc Generating and implementing a structural variation graph genome
CN116024320A (en) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 Fluorescent quantitative PCR method for detecting nucleic acid
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
US20240038327A1 (en) 2022-07-26 2024-02-01 Illumina Software, Inc. Rapid single-cell multiomics processing using an executable file
US20250361558A1 (en) 2022-08-19 2025-11-27 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
EP4595060A1 (en) 2022-09-29 2025-08-06 Illumina, Inc. A target-variant-reference panel for imputing target variants
US20240141427A1 (en) 2022-09-30 2024-05-02 Illumina, Inc. Polymerases, compositions, and methods of use
CN120153070A (en) 2022-09-30 2025-06-13 因美纳有限公司 Helicase-cytidine deaminase complexes and methods of use thereof
WO2024073519A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Machine-learning model for refining structural variant calls
EP4594482A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20250388894A1 (en) 2022-09-30 2025-12-25 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024077096A1 (en) 2022-10-05 2024-04-11 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
EP4599080A2 (en) 2022-10-06 2025-08-13 Illumina, Inc. Probes for improving environmental sample surveillance
WO2024077162A2 (en) 2022-10-06 2024-04-11 Illumina, Inc. Probes for improving coronavirus sample surveillance
WO2024077152A1 (en) 2022-10-06 2024-04-11 Illumina, Inc. Probes for depleting abundant small noncoding rna
US20240127906A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
KR20250110272A (en) 2022-11-15 2025-07-18 가던트 헬쓰, 인크. Methods for predicting non-small cell lung cancer (NSCLC) patient drug response or time to death or cancer progression using signals from both baseline CTDNA levels and longitudinal changes in CTDNA levels over time from circulating tumor DNA (CTDNA)
EP4627113A1 (en) 2022-11-30 2025-10-08 Illumina, Inc. Chemoenzymatic correction of false positive uracil transformations
EP4627583A1 (en) 2022-11-30 2025-10-08 Illumina, Inc. Accurately predicting variants from methylation sequencing data
WO2024129672A1 (en) 2022-12-12 2024-06-20 The Broad Institute, Inc. Trafficked rnas for assessment of cell-cell connectivity and neuroanatomy
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
US20250354196A1 (en) 2022-12-16 2025-11-20 Illumina, Inc. Boranes on solid supports
KR20250138733A (en) 2022-12-21 2025-09-22 가던트 헬쓰, 인크. Detection of homologous recombination deficiency based on the methylation status of cell-free nucleic acid molecules.
AU2023409219A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
CA3246536A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
CN119095984A (en) 2022-12-27 2024-12-06 伊路米纳有限公司 Sequencing method using 3' allyl-terminated nucleotides
CA3246669A1 (en) 2023-01-06 2024-07-11 Illumina, Inc. Reducing uracils by polymerase
WO2024151373A1 (en) 2023-01-12 2024-07-18 Nautilus Subsidiary, Inc. Characterization of glycans and glycoconjugates
JP2026506415A (en) 2023-02-06 2026-02-25 イルミナ インコーポレイテッド Determination and removal of intercluster optical interference
CA3259901A1 (en) 2023-02-17 2024-08-22 Illumina, Inc. Cell-free dna signals as biomarkers of preeclampsia
EP4706045A1 (en) 2023-03-30 2026-03-11 Illumina, Inc. Tandem repeat genotyping
EP4689181A1 (en) 2023-03-30 2026-02-11 Illumina, Inc. Compositions and methods for nucleic acid sequencing
CA3260664A1 (en) 2023-05-03 2024-11-07 Illumina, Inc. Machine learning model for recalibrating genotype calls from existing sequencing data files
EP4705522A1 (en) 2023-05-05 2026-03-11 Guardant Health, Inc. Cell-free dna blood-based test for cancer screening
EP4720334A1 (en) 2023-05-26 2026-04-08 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2024254003A1 (en) 2023-06-05 2024-12-12 Illumina, Inc. Identification and mapping of methylation sites
WO2024259251A1 (en) 2023-06-15 2024-12-19 Guardant Health, Inc. Method for hrd detection in targeted cfdna samples using de novo mutational signatures
EP4731786A1 (en) 2023-06-26 2026-04-29 Pacific Biosciences of California, Inc. Compositions and methods for nucleic acid extension
CN121359206A (en) 2023-06-27 2026-01-16 因美纳有限公司 Variant detection using methylation level estimation
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
AU2024311047A1 (en) 2023-06-30 2025-12-11 Illumina, Inc. Using flowcell spatial coordinates to link reads for improved genome analysis
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
GB202312147D0 (en) 2023-08-08 2023-09-20 Syndex Bio Ltd Methylation method
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
WO2025059533A1 (en) 2023-09-13 2025-03-20 The Broad Institute, Inc. Crispr enzymes and systems
WO2025072368A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Capture and selective release of biological material
WO2025072783A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
US20250111898A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
US20250246266A1 (en) 2023-09-29 2025-07-31 Guardant Health, Inc. Genotyping cyp2d6
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
WO2025090646A1 (en) 2023-10-27 2025-05-01 Guardant Health, Inc. Monitoring molecular response by allelic imbalance
WO2025106629A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Structural variant detection using spatially linked reads
WO2025106431A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Determining structural variants
WO2025117738A1 (en) 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency
EP4567128A1 (en) 2023-12-07 2025-06-11 Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft Improved method and means for spatial nucleic acid detection in-situ
WO2025129074A2 (en) 2023-12-14 2025-06-19 Illumina, Inc. Indexing techniques for tagmented dna libraries
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
US20250201346A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137268A1 (en) 2023-12-20 2025-06-26 Pacific Biosciences Of California, Inc. Methods and compositions for reducing gc bias
US20250210137A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
CN121359207A (en) 2023-12-21 2026-01-16 因美纳有限公司 Enhanced nucleotide read mapping and alignment using improved haplotype data structures with allele-variant differences.
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
CN117809757B (en) * 2023-12-28 2024-10-01 苏州腾迈医药科技有限公司 Molecular force field fitting method and device
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
CN121359209A (en) 2024-02-28 2026-01-16 因美纳有限公司 Personalized haplotype database for improving nucleotide read mapping and alignment, and for improving genotype detection.
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025189105A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Size thresholding of dna fragments
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
WO2025207886A1 (en) 2024-03-28 2025-10-02 Illumina, Inc. Kits and methods for on-flow cell library preparation and methylation detection
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis
CN121359208A (en) 2024-05-13 2026-01-16 因美纳有限公司 The sequencing cycle was modified during sequencing run to meet customized coverage estimation for the target genome region.
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2026006771A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Cluster-filtering scores
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
WO2026030370A1 (en) 2024-07-30 2026-02-05 Illumina, Inc. Generating adaptive intensity value decision boundaries for nucleotide sequencing
WO2026072725A1 (en) 2024-09-25 2026-04-02 Illumina, Inc. Adjusting oligonucleotide-cluster location in images in real time using band-edge spectral regions
WO2026072464A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Read mapping enhanced by spatial information
WO2026072262A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Methods and systems for phasing, mapping, and assembling sequence reads and detecting structural variants
WO2026072436A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Systems and methods for quantifying sequence read connectivity
WO2026072986A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity sequences using a machine learning model
WO2026072263A1 (en) 2024-09-27 2026-04-02 Illumina, Inc. Methods and systems for detecting copy number variants
WO2026073084A2 (en) 2024-09-30 2026-04-02 Illumina, Inc. Dna enrichment methods and kits
WO2026073213A1 (en) 2024-09-30 2026-04-02 Illumina, Inc. Protein capture techniques with surface linked aptamers
WO2026080196A1 (en) 2024-10-09 2026-04-16 Illumina, Inc. Machine learning methods and systems for detecting structural variants
WO2026080827A1 (en) 2024-10-11 2026-04-16 Illumina, Inc. Tagmentation kits and methods
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026096395A1 (en) 2024-10-28 2026-05-07 Illumina, Inc. Methods and compositions for preparing samples for sequencing
WO2026096634A1 (en) 2024-10-30 2026-05-07 Illumina, Inc. On-flow cell processing of a complementary dna sample
WO2026096262A1 (en) 2024-10-30 2026-05-07 Illumina, Inc. Methods and systems for phasing sequence reads
WO2026096180A1 (en) 2024-10-30 2026-05-07 Illumina, Inc. Transposome complex attachment to flow cell surfaces

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031834A1 (en) * 1997-01-15 1998-07-23 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
WO2000017330A1 (en) * 1998-09-21 2000-03-30 Shangai Institute Of Biochemistry Chinese Academy Of Sciences Dna polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US6207421B1 (en) * 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US5230781A (en) * 1984-03-29 1993-07-27 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US5366603A (en) * 1984-03-29 1994-11-22 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes
US4997928A (en) * 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5232075A (en) * 1991-06-25 1993-08-03 New Venture Gear, Inc. Viscous coupling apparatus with coined plates
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
JP3227189B2 (en) * 1991-12-25 2001-11-12 キヤノン株式会社 Apparatus provided with flexible cable and ink jet recording apparatus provided with the apparatus
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
CA2153387A1 (en) * 1993-01-07 1994-07-21 Hubert Koester Dna sequencing by mass spectrometry
US5677196A (en) * 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
WO1995006138A1 (en) * 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6593148B1 (en) * 1994-03-01 2003-07-15 Li-Cor, Inc. Cyanine dye compounds and labeling methods
US5601982A (en) * 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US5961923A (en) * 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5661028A (en) * 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
EP1009802B1 (en) * 1997-02-12 2004-08-11 Eugene Y. Chan Methods for analyzimg polymers
CA2283789C (en) * 1997-03-12 2005-07-05 Applied Biosystems, Llc Dna polymerases having improved labeled nucleotide incorporation properties
DK1017848T3 (en) 1997-07-28 2003-02-10 Medical Biosystems Ltd Nucleic acid sequence analysis
CA2330673C (en) * 1998-05-01 2009-05-26 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6280939B1 (en) * 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
DE19844931C1 (en) * 1998-09-30 2000-06-15 Stefan Seeger Procedures for DNA or RNA sequencing
US6221592B1 (en) * 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6044744A (en) * 1998-10-29 2000-04-04 At&T Corp. Fiber optic cable sheath removal tool
JP4638043B2 (en) * 1998-12-14 2011-02-23 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド Systems and methods for sequencing single molecule nucleic acids by polymerase synthesis
TW461073B (en) 1998-12-15 2001-10-21 Matsushita Electric Industrial Co Ltd Clock phase adjust method, and integrated circuit and design method of the same
US6558945B1 (en) * 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
EP1159453B1 (en) * 1999-03-10 2008-05-28 ASM Scientific, Inc. A method for direct nucleic acid sequencing
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
AU7086800A (en) * 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6399335B1 (en) * 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6917726B2 (en) * 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6869764B2 (en) * 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
ATE377093T1 (en) * 2000-07-07 2007-11-15 Visigen Biotechnologies Inc REAL-TIME SEQUENCE DETERMINATION
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
JP4989013B2 (en) * 2000-09-19 2012-08-01 リ−コール インコーポレーティッド Cyanine dye
US7211414B2 (en) * 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7068773B2 (en) * 2001-04-25 2006-06-27 Tekelec Methods and systems for load sharing signaling messages among signaling links in networks utilizing international signaling protocols
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US20030064400A1 (en) * 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7005518B2 (en) * 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
JP4896708B2 (en) * 2003-02-05 2012-03-14 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Terminal phosphate-labeled nucleotides with novel linkers
JP2006513705A (en) * 2003-02-05 2006-04-27 アマシャム・バイオサイエンス・コーポレイション Nucleic acid growth
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
KR100558528B1 (en) * 2003-09-25 2006-03-10 동부아남반도체 주식회사 CMOS image sensor and its manufacturing method
US7170050B2 (en) * 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20060062531A1 (en) * 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
EP1846758A2 (en) * 2005-02-09 2007-10-24 Pacific Biosciences of California, Inc. Nucleotide compositions and uses thereof
US7130041B2 (en) * 2005-03-02 2006-10-31 Li-Cor, Inc. On-chip spectral filtering using CCD array for imaging and spectroscopy
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
US7509836B2 (en) * 2005-09-01 2009-03-31 Li-Cor, Inc. Gas flux system chamber design and positioning method
US7935310B2 (en) * 2005-11-28 2011-05-03 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrate and methods for making and using same
JP2009519717A (en) * 2005-12-16 2009-05-21 アプレラ コーポレイション Method and system for fixed phase sequencing
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031834A1 (en) * 1997-01-15 1998-07-23 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
WO2000017330A1 (en) * 1998-09-21 2000-03-30 Shangai Institute Of Biochemistry Chinese Academy Of Sciences Dna polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FUREY W SCOTT ET AL: "Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 37, no. 9, 3 March 1998 (1998-03-03), pages 2979 - 2990, XP002171248, ISSN: 0006-2960 *

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7323320B2 (en) 2002-09-12 2008-01-29 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US9709503B2 (en) 2004-09-17 2017-07-18 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US11705217B2 (en) 2008-03-28 2023-07-18 Pacific Biosciences Of California, Inc. Sequencing using concatemers of copies of sense and antisense strands
US9365839B2 (en) 2009-03-27 2016-06-14 Life Technologies Corporation Polymerase compositions and methods
US9567629B2 (en) 2009-03-27 2017-02-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
US9365838B2 (en) 2009-03-27 2016-06-14 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US10246744B2 (en) 2016-08-15 2019-04-02 Omniome, Inc. Method and system for sequencing nucleic acids
US10443098B2 (en) 2016-08-15 2019-10-15 Omniome, Inc. Method and system for sequencing nucleic acids
US11168364B2 (en) 2016-08-15 2021-11-09 Omniome, Inc. Method and system for sequencing nucleic acids
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10655176B2 (en) 2017-04-25 2020-05-19 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US11447823B2 (en) 2017-04-25 2022-09-20 Pacific Biosciences Of California, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US12180545B2 (en) 2017-04-25 2024-12-31 Pacific Biosciences Of California, Inc. Methods and apparatus that increase sequencing-by-binding efficiency

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