WO2002006463A3 - Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches - Google Patents

Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches Download PDF

Info

Publication number
WO2002006463A3
WO2002006463A3 PCT/EP2001/008190 EP0108190W WO0206463A3 WO 2002006463 A3 WO2002006463 A3 WO 2002006463A3 EP 0108190 W EP0108190 W EP 0108190W WO 0206463 A3 WO0206463 A3 WO 0206463A3
Authority
WO
WIPO (PCT)
Prior art keywords
monomeric
chain
regulation
switches
gene expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2001/008190
Other languages
French (fr)
Other versions
WO2002006463A2 (en
Inventor
Roger Beerli
Ulrich Schopfer
Carlos F Barbas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Pharma GmbH
Novartis AG
Scripps Research Institute
Original Assignee
Novartis Erfindungen Verwaltungs GmbH
Novartis AG
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to IL15383301A priority Critical patent/IL153833A0/en
Application filed by Novartis Erfindungen Verwaltungs GmbH, Novartis AG, Scripps Research Institute filed Critical Novartis Erfindungen Verwaltungs GmbH
Priority to NZ523649A priority patent/NZ523649A/en
Priority to JP2002512356A priority patent/JP2004504029A/en
Priority to EP01951681A priority patent/EP1303606B1/en
Priority to CA002415726A priority patent/CA2415726A1/en
Priority to AU2001272544A priority patent/AU2001272544B2/en
Priority to DE60132208T priority patent/DE60132208T2/en
Priority to AU7254401A priority patent/AU7254401A/en
Publication of WO2002006463A2 publication Critical patent/WO2002006463A2/en
Publication of WO2002006463A3 publication Critical patent/WO2002006463A3/en
Anticipated expiration legal-status Critical
Priority to AU2005234684A priority patent/AU2005234684B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C07K2319/715—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16 containing a domain for ligand dependent transcriptional activation, e.g. containing a steroid receptor domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Single chain, monomeric polypeptide gene switches are provided. The gene switches include ligand binding domains and at least one functional domain. Preferred functional domains are DNA binding domains and transcriptional regulating domains. Methods of regulating gene function using the switches are also provided.
PCT/EP2001/008190 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches Ceased WO2002006463A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AU7254401A AU7254401A (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
NZ523649A NZ523649A (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
JP2002512356A JP2004504029A (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand-dependent polypeptide switches
EP01951681A EP1303606B1 (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
CA002415726A CA2415726A1 (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
IL15383301A IL153833A0 (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
DE60132208T DE60132208T2 (en) 2000-07-18 2001-07-16 REGULATION OF GENE EXPRESSION USING ONE-CHAIN, MONOMER, LIGAND-DEPENDENT POLYPEPTIDE SWITCHES
AU2001272544A AU2001272544B2 (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
AU2005234684A AU2005234684B2 (en) 2000-07-18 2005-11-18 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61906300A 2000-07-18 2000-07-18
US09/619,063 2000-07-18

Publications (2)

Publication Number Publication Date
WO2002006463A2 WO2002006463A2 (en) 2002-01-24
WO2002006463A3 true WO2002006463A3 (en) 2002-07-25

Family

ID=24480304

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/008190 Ceased WO2002006463A2 (en) 2000-07-18 2001-07-16 Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches

Country Status (10)

Country Link
EP (2) EP1908835A3 (en)
JP (1) JP2004504029A (en)
AT (1) ATE382691T1 (en)
AU (2) AU7254401A (en)
CA (1) CA2415726A1 (en)
DE (1) DE60132208T2 (en)
ES (1) ES2299494T3 (en)
IL (1) IL153833A0 (en)
NZ (1) NZ523649A (en)
WO (1) WO2002006463A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329728B1 (en) 1999-10-25 2008-02-12 The Scripps Research Institute Ligand activated transcriptional regulator proteins
US7011972B2 (en) 2000-07-18 2006-03-14 The Scripps Research Institute Fusion polypeptide comprising two ligand binding domains
EP1967590A3 (en) 2001-03-13 2008-09-17 Novartis AG Lentiviral packaging constructs
WO2002097050A2 (en) 2001-05-31 2002-12-05 Novartis Ag Novel estrogen receptor ligand binding domain variants and novel ligands and pharmaceutical compositions
US7927840B2 (en) 2006-09-11 2011-04-19 Gen Probe Incorporated Method for detecting West Nile Virus nucleic acids in the 3′ non-coding region
AU2003298605B2 (en) 2002-10-16 2007-10-18 Gen-Probe Incorporated Compositions and methods for detecting west nile virus
EP4210733B1 (en) * 2020-09-04 2024-11-06 Miltenyi Biotec B.V. & Co. KG System for inducible expression of an adapter in immune cells

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0540065A1 (en) * 1987-12-02 1993-05-05 The Salk Institute For Biological Studies Retinoic acid receptor composition
WO1998018925A2 (en) * 1996-10-29 1998-05-07 Valentis, Inc. Modified glucocorticoid receptors, glucocorticoid receptors/progesterone receptors hybrids
WO1999010510A2 (en) * 1997-08-26 1999-03-04 Ariad Gene Therapeutics, Inc. Fusion proteins comprising a dimerization, trimerization or tetramerization domain and an additional heterologous transcription activation, transcription repression, dna binding or ligand binding domain
WO1999047656A2 (en) * 1998-03-17 1999-09-23 Gendaq Limited Nucleic acid binding proteins
WO2000023464A2 (en) * 1998-10-16 2000-04-27 Novartis Ag Zinc finger binding domains for gnn
WO2001030843A1 (en) * 1999-10-25 2001-05-03 Novartis Ag Ligand activated transcriptional regulator proteins

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7057015B1 (en) * 1999-10-20 2006-06-06 The Salk Institute For Biological Studies Hormone receptor functional dimers and methods of their use

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0540065A1 (en) * 1987-12-02 1993-05-05 The Salk Institute For Biological Studies Retinoic acid receptor composition
WO1998018925A2 (en) * 1996-10-29 1998-05-07 Valentis, Inc. Modified glucocorticoid receptors, glucocorticoid receptors/progesterone receptors hybrids
WO1999010510A2 (en) * 1997-08-26 1999-03-04 Ariad Gene Therapeutics, Inc. Fusion proteins comprising a dimerization, trimerization or tetramerization domain and an additional heterologous transcription activation, transcription repression, dna binding or ligand binding domain
WO1999047656A2 (en) * 1998-03-17 1999-09-23 Gendaq Limited Nucleic acid binding proteins
WO2000023464A2 (en) * 1998-10-16 2000-04-27 Novartis Ag Zinc finger binding domains for gnn
WO2001030843A1 (en) * 1999-10-25 2001-05-03 Novartis Ag Ligand activated transcriptional regulator proteins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEERLI ROGER R ET AL: "Chemically regulated zinc finger transcription factors.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 42, 20 October 2000 (2000-10-20), pages 32617 - 32627, XP002199787, ISSN: 0021-9258 *
BEERLI ROGER R ET AL: "Positive and negative regulation of endogenous genes by designed transcription factors", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 97, no. 4, 15 February 2000 (2000-02-15), pages 1495 - 1500, XP002188512, ISSN: 0027-8424 *
BURCIN M M ET AL: "A REGULATORY SYSTEM FOR TARGET GENE EXPRESSION", FRONTIERS IN BIOSCIENCE, XX, XX, vol. 3, 1 March 1998 (1998-03-01), pages 1 - 7, XP000874619 *

Also Published As

Publication number Publication date
CA2415726A1 (en) 2002-01-24
AU7254401A (en) 2002-01-30
NZ523649A (en) 2005-02-25
DE60132208D1 (en) 2008-02-14
AU2001272544B2 (en) 2005-12-01
ATE382691T1 (en) 2008-01-15
JP2004504029A (en) 2004-02-12
IL153833A0 (en) 2003-07-31
DE60132208T2 (en) 2008-12-24
EP1908835A3 (en) 2008-06-18
EP1303606B1 (en) 2008-01-02
EP1303606A2 (en) 2003-04-23
WO2002006463A2 (en) 2002-01-24
ES2299494T3 (en) 2008-06-01
EP1908835A2 (en) 2008-04-09

Similar Documents

Publication Publication Date Title
WO2001053312A8 (en) Novel nucleic acids and polypeptides
CA2383926A1 (en) Functional genomics using zinc finger proteins
EP0892048A3 (en) XAF genes and polypeptides and their use for modulating apoptosis
WO2001023412A3 (en) Methods of screening for agents which inhibit aggregation of polypeptides
AU4141101A (en) Nucleic acids, proteins, and antibodies
WO1999025843A3 (en) Human checkpoint kinase, hcds1, compositions and methods
AU2003234198A1 (en) Novel genes, compositions, and methods for modulating the unfolded protein response
WO2002044376A3 (en) Modulation of gene expression using insulator binding proteins
WO2001049721A3 (en) Bacterial genes and proteins that are essential for cell viability and their uses
WO2003066821A3 (en) Molecules interacting with casl (mical) polynucleotides, polypeptides, and methods of using the same
ZA9710163B (en) Novel carboxylesterase nucleic acid molecules, proteins and uses thereof.
WO2002066640A3 (en) Zinc finger binding domains for nucleotide sequence ann
AU1178100A (en) Novel g protein-coupled receptor protein, its dna and ligand thereof
AU7254401A (en) Regulation of gene expression using single-chain, monomeric, ligand dependent polypeptide switches
AU2001265096A1 (en) Short-root gene, promoter, and uses thereof
AU5245900A (en) Novel protein and dna thereof
WO2001064837A3 (en) β NETRIN AND USES THEREOF
WO1999039009A8 (en) Processes for isolating, amplifying and characterizing dna
WO2002026960A3 (en) Modulation of gene expression using localization domains
WO1997046587A3 (en) Ob protein binding protein
AU4942597A (en) Thermostable dna polymerase from (anaerocellum thermophilum)
WO2001016605A3 (en) Identifying modulators of transcriptional activator protein interactions
WO2001068846A3 (en) Transcription activator of the cited family
WO1999028465A3 (en) Pias molecules that recognize and bind stat proteins and uses thereof
AU1457300A (en) Dna replication-regulating genes, antibodies thereto and diagnostic applicationsthereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2001951681

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 153833

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 2415726

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001272544

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 523649

Country of ref document: NZ

WWP Wipo information: published in national office

Ref document number: 2001951681

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 523649

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 523649

Country of ref document: NZ

WWG Wipo information: grant in national office

Ref document number: 2001272544

Country of ref document: AU

WWG Wipo information: grant in national office

Ref document number: 2001951681

Country of ref document: EP