WO2002012566A3 - The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix - Google Patents

The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix Download PDF

Info

Publication number
WO2002012566A3
WO2002012566A3 PCT/US2001/024894 US0124894W WO0212566A3 WO 2002012566 A3 WO2002012566 A3 WO 2002012566A3 US 0124894 W US0124894 W US 0124894W WO 0212566 A3 WO0212566 A3 WO 0212566A3
Authority
WO
WIPO (PCT)
Prior art keywords
photocycloaddition
immobilization
oligonucleotides
evaluation
hydrogel matrix
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/024894
Other languages
French (fr)
Other versions
WO2002012566A2 (en
Inventor
Robert Elghanian
Charles K Brush
Yanzheng Xu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Motorola Solutions Inc
Original Assignee
Motorola Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Motorola Inc filed Critical Motorola Inc
Priority to EP01957507A priority Critical patent/EP1307414A2/en
Priority to AU2001279244A priority patent/AU2001279244A1/en
Publication of WO2002012566A2 publication Critical patent/WO2002012566A2/en
Publication of WO2002012566A3 publication Critical patent/WO2002012566A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

[0064] [2+2]photo-attachable functional groups were incorporated in polyacrylamide based hydrogels and synthetic oligonucleotide probes. The probes were photochemically attached by covalent bonding to the three dimensional surface of a hydrogel. The resultant hydrogel microarrays were used for detection of specific target oligonulcleotides, including mRNA and DNA, as suitable for expression and single nucleotide polymorphism type microarray analyses.
PCT/US2001/024894 2000-08-09 2001-08-09 The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix Ceased WO2002012566A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01957507A EP1307414A2 (en) 2000-08-09 2001-08-09 The use and evaluation of a 2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
AU2001279244A AU2001279244A1 (en) 2000-08-09 2001-08-09 The use and evaluation of a (2+2) photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US22407000P 2000-08-09 2000-08-09
US60/224,070 2000-08-09
US23230500P 2000-09-12 2000-09-12
US60/232,305 2000-09-12

Publications (2)

Publication Number Publication Date
WO2002012566A2 WO2002012566A2 (en) 2002-02-14
WO2002012566A3 true WO2002012566A3 (en) 2003-01-09

Family

ID=26918399

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/024894 Ceased WO2002012566A2 (en) 2000-08-09 2001-08-09 The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix

Country Status (3)

Country Link
EP (1) EP1307414A2 (en)
AU (1) AU2001279244A1 (en)
WO (1) WO2002012566A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025049700A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6921638B2 (en) 1999-06-25 2005-07-26 Amersham Biosciences Ab Hydrogel-based microarray signal amplification methods and devices therefor
US6664061B2 (en) * 1999-06-25 2003-12-16 Amersham Biosciences Ab Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
DK2964624T3 (en) 2013-03-08 2017-04-03 Illumina Cambridge Ltd RHODAMINE COMPOUNDS AND USE AS FLUORESCING LABELS
ES2620423T3 (en) 2013-03-08 2017-06-28 Illumina Cambridge Limited Polymetin compounds and their use as fluorescent markers
DK3017065T3 (en) 2013-07-01 2018-11-26 Illumina Inc Catalyst-free Surface functionalization and polymer grafting
CN114089597B (en) 2013-12-19 2025-03-14 Illumina公司 Substrate comprising a nano-patterned surface and method for preparing the same
US10537889B2 (en) 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
EP3325648B1 (en) 2015-07-17 2023-03-29 Illumina, Inc. Polymer sheets for sequencing applications
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
SG11201810611YA (en) 2016-12-22 2018-12-28 Illumina Cambridge Ltd Coumarin compounds and their uses as fluorescent labels
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
GB201716931D0 (en) 2017-10-16 2017-11-29 Illumina Cambridge Ltd New fluorescent compounds and their use as biomarkers
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
BR112020026528B1 (en) 2019-03-01 2024-02-15 Illumina Cambridge Limited COUMARIN COMPOUND REPLACED WITH EXOCYCLIC AMINE, NUCLEOTIDE OR OLIGONUCLEOTIDE, KIT, SEQUENCING METHOD AND METHOD TO SYNTHESIS THE SAID COMPOUND
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
CA3103636A1 (en) 2019-03-01 2020-09-10 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and their uses as fluorescent labels
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
JP2023510454A (en) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド Pigments and compositions containing cyclooctatetraene
US11787831B2 (en) 2020-06-22 2023-10-17 Illumina Cambridge Limited Nucleosides and nucleotides with 3′ acetal blocking group
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195518A1 (en) 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
JP2024516191A (en) 2021-05-05 2024-04-12 イルミナ ケンブリッジ リミテッド Fluorescent dyes containing bis-boron fused heterocycles and their use in sequencing - Patents.com
KR20240009435A (en) 2021-05-20 2024-01-22 일루미나, 인코포레이티드 Compositions and methods for sequencing by synthesis
EP4448786B1 (en) 2021-12-16 2026-02-04 Illumina, Inc. Methods for metal directed cleavage of surface-bound polynucleotides
AU2022421156A1 (en) 2021-12-20 2024-01-18 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
EP4453243B1 (en) 2021-12-20 2026-02-11 Illumina, Inc. Periodate compositions and methods for chemical cleavage of surface-bound polynucleotides
US20230304086A1 (en) 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
AU2023246772A1 (en) 2022-03-29 2024-01-18 Illumina Inc. Chromenoquinoline dyes and uses in sequencing
CA3223037A1 (en) 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
WO2023192900A1 (en) 2022-03-31 2023-10-05 Illumina Singapore Pte. Ltd. Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis
CN119301275A (en) 2022-05-31 2025-01-10 伊路米纳有限公司 Compositions and methods for nucleic acid sequencing
CN119604513A (en) 2022-06-28 2025-03-11 因美纳公司 Fluorescent dyes containing fused tetracyclic biboronic heterocycles and their use in sequencing
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
US20240140939A1 (en) 2022-09-30 2024-05-02 Illumina Cambridge Limited Compositions and methods for reducing photo damage during sequencing
WO2024123866A1 (en) 2022-12-09 2024-06-13 Illumina, Inc. Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers
WO2024137765A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
CN119095982A (en) 2022-12-22 2024-12-06 伊路米纳有限公司 Palladium catalyst compositions and methods for sequencing by synthesis
CN119095984A (en) 2022-12-27 2024-12-06 伊路米纳有限公司 Sequencing method using 3' allyl-terminated nucleotides
WO2024206407A2 (en) 2023-03-29 2024-10-03 Illumina, Inc. Naphthalimide dyes and uses in nucleic acid sequencing
EP4689181A1 (en) 2023-03-30 2026-02-11 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2024254367A1 (en) 2023-06-09 2024-12-12 Illumina, Inc Method for polishing a substrate
WO2025090596A1 (en) 2023-10-26 2025-05-01 Illumina, Inc. 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing
US20250163505A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Conjugated polymers or polymer dots as fluorescent labels
US20250197933A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Hydrogel nanoparticles as labeling scaffold in sequencing
WO2025144716A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
US20250215494A1 (en) 2023-12-29 2025-07-03 Illumina, Inc. Tricyclic polymethine dyes for nucleic acid sequencing
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025221895A1 (en) 2024-04-19 2025-10-23 Illumina, Inc. Water soluble polymer scaffolds for dye labeling
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015798A1 (en) * 1989-06-19 1990-12-27 Boehringer Mannheim Gmbh Aminoalkyl maleimides and haptene and antigen derivatives thereof and conjugates with peptides or proteins
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces
WO1998030575A1 (en) * 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
WO2000034457A1 (en) * 1998-12-10 2000-06-15 Takara Shuzo Co., Ltd. Method for immobilizing oligonucleotide on a carrier
WO2001001143A2 (en) * 1999-06-25 2001-01-04 Motorola Inc. Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker
WO2001084234A1 (en) * 2000-05-01 2001-11-08 Proligo Llc Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990015798A1 (en) * 1989-06-19 1990-12-27 Boehringer Mannheim Gmbh Aminoalkyl maleimides and haptene and antigen derivatives thereof and conjugates with peptides or proteins
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces
WO1998030575A1 (en) * 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of macromolecules
WO2000034457A1 (en) * 1998-12-10 2000-06-15 Takara Shuzo Co., Ltd. Method for immobilizing oligonucleotide on a carrier
WO2001001143A2 (en) * 1999-06-25 2001-01-04 Motorola Inc. Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker
WO2001084234A1 (en) * 2000-05-01 2001-11-08 Proligo Llc Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 200036, Derwent World Patents Index; Class B04, AN 2000-423411, XP002203841 *
ELSNER H I ET AL: "USE OF PSORALENS FOR COVALENT IMMOBILIZATION OF BIOMOLECULES IN SOLID PHASE ASSAYS", BIOCONJUGATE CHEMISTRY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, US, vol. 5, no. 5, 1 September 1994 (1994-09-01), pages 463 - 467, XP000465957, ISSN: 1043-1802 *
M. N. YOUSAF, M. MRKSICH: "Diels-Alder reaction for the selective immobilization of protein to electroactive self-assembled monolayers", J. AM. CHEM. SOC., vol. 121, 1999, pages 4286 - 4287, XP002203840 *
REHMAN F ET AL: "Immobilization of acrylamide-modified oligonucleotides by co-polymerization", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 2, 15 February 1999 (1999-02-15), pages 649 - 655, XP002155808, ISSN: 0305-1048 *
YI-ZHONG A ET AL: "Sequence-Specific Modification of Guanosine in DNA by a C60-Linked Deoxyoligonucleotide: Evidence for a Non-Singlet Oxygen Mechanism", TETRAHEDRON, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 52, no. 14, 1 April 1996 (1996-04-01), pages 5179 - 5189, XP004104270, ISSN: 0040-4020 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025049700A1 (en) 2023-08-31 2025-03-06 Illumina, Inc. Compositions and methods for nucleic acid sequencing

Also Published As

Publication number Publication date
WO2002012566A2 (en) 2002-02-14
EP1307414A2 (en) 2003-05-07
AU2001279244A1 (en) 2002-02-18

Similar Documents

Publication Publication Date Title
WO2002012566A3 (en) The use and evaluation of a [2+2] photocycloaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
WO2003014392A3 (en) Use and evaluation of a [2+2] photoaddition in immobilization of oligonucleotides on a three-dimensional hydrogel matrix
EP0971943B1 (en) Nucleic acid-containing polymerizable complex
US7364848B2 (en) Integrated microarray analysis
US6057100A (en) Oligonucleotide arrays
WO2001036682A3 (en) Long oligonucleotide arrays
US6692912B1 (en) Nucleic acid-containing polymerizable complex
NZ525336A (en) Leukocyte expression profiling
EP1693469A3 (en) Detecting DNA sequence variations
CA2278715A1 (en) Aminooxy-modified oligonucleotides
AU3754289A (en) Immobilized sequence-specific probes
GB2389904A (en) Arrayed polynucleotides and their use in genome analysis
CA2206102A1 (en) Comparative fluorescence hybridization to nucleic acid arrays
BR0112789A (en) Reaction cartridge, device for performing enzymatic and / or molecular biology reactions, and nucleic acid amplification and filler processes in a cartridge's reaction chambers
JP2002544508A5 (en)
CA2301403A1 (en) High-throughput screening of gene function using libraries for functional genomics applications
WO2000070088A3 (en) Immobilization of unmodified biopolymers to acyl fluoride activated substrates
CA2189941A1 (en) Fluorescence Polarization Detection of Nucleic Acids
WO2000075792A3 (en) Highly sensitive biological agent probe
CA2195971A1 (en) Nucleic acid hybridization assay probes targeted to mycoplasma pneumoniae nucleic acid
NO20032697L (en) Test strip assay system and assay method for detection and / or determination of specific nucleic acid sequences
WO2005040419A8 (en) Oligonucleotide microarray
WO2003054149A3 (en) Targeted genetic risk-stratification using microarrays
ATE415491T1 (en) OLIGONUCLEOTIDES FOR LABELING OLIGONUCLEOTIDE PROBE AND PROTEINS
US20040081969A1 (en) Devices and methods for evaulating the quality of a sample for use in an array assay

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2001957507

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001957507

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2001957507

Country of ref document: EP