WO2002021116A3 - Electrophoresis cell for multiple slab gels - Google Patents

Electrophoresis cell for multiple slab gels Download PDF

Info

Publication number
WO2002021116A3
WO2002021116A3 PCT/US2001/027572 US0127572W WO0221116A3 WO 2002021116 A3 WO2002021116 A3 WO 2002021116A3 US 0127572 W US0127572 W US 0127572W WO 0221116 A3 WO0221116 A3 WO 0221116A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
gels
buffer solution
slab gels
slab
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/027572
Other languages
French (fr)
Other versions
WO2002021116A2 (en
Inventor
George Fernwood
Christina Whitman
Adriana J Harbers
Evelio Perez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Priority to JP2002524687A priority Critical patent/JP3917515B2/en
Priority to EP01968542A priority patent/EP1315961B1/en
Priority to DE0001315961T priority patent/DE01968542T1/en
Priority to CA2396970A priority patent/CA2396970C/en
Priority to AU88784/01A priority patent/AU769397B2/en
Publication of WO2002021116A2 publication Critical patent/WO2002021116A2/en
Publication of WO2002021116A3 publication Critical patent/WO2002021116A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Electrostatic Separation (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Electrophoresis is performed simultaneously in a multitude of slab gels in a single cell that supports the slab gels vertically and parallel to each other while immersed in a buffer solution, and that applies a voltage to all gels simultaneously through a single pair of plate electrodes. Temperature control is achieved by circulating the buffer solution upward through the cell, entering near the base of each slab gel and leaving near the top, and cooling the circulating buffer solution with a tube heat exchanger positioned on the floor of the cell.
PCT/US2001/027572 2000-09-06 2001-09-04 Electrophoresis cell for multiple slab gels Ceased WO2002021116A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2002524687A JP3917515B2 (en) 2000-09-06 2001-09-04 Multiple slab gel electrophoresis cells
EP01968542A EP1315961B1 (en) 2000-09-06 2001-09-04 Electrophoresis cell for multiple slab gels
DE0001315961T DE01968542T1 (en) 2000-09-06 2001-09-04 ELECTROFORETIC CELL FOR MULTIPLE GEL PLATES
CA2396970A CA2396970C (en) 2000-09-06 2001-09-04 Electrophoresis cell for multiple slab gels
AU88784/01A AU769397B2 (en) 2000-09-06 2001-09-04 Electrophoresis cell for multiple slab gels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/655,593 US6451193B1 (en) 2000-09-06 2000-09-06 Electrophoresis cell for multiple slab gels
US09/655,593 2000-09-06

Publications (2)

Publication Number Publication Date
WO2002021116A2 WO2002021116A2 (en) 2002-03-14
WO2002021116A3 true WO2002021116A3 (en) 2003-01-03

Family

ID=24629528

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/027572 Ceased WO2002021116A2 (en) 2000-09-06 2001-09-04 Electrophoresis cell for multiple slab gels

Country Status (7)

Country Link
US (1) US6451193B1 (en)
EP (1) EP1315961B1 (en)
JP (1) JP3917515B2 (en)
AU (1) AU769397B2 (en)
CA (1) CA2396970C (en)
DE (1) DE01968542T1 (en)
WO (1) WO2002021116A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6942775B1 (en) * 2002-05-24 2005-09-13 Owl Separation Systems, Inc. Vertical electrophoresis system
EP1664754A4 (en) * 2003-09-22 2010-08-25 Life Technologies Corp Apparatus for concurrent electrophoresis in a plurality of gels
WO2005098408A1 (en) * 2004-04-01 2005-10-20 C.B.S. Scientific Co., Inc. Multi function gel electrophoresis and apparatus
US20060254917A1 (en) * 2005-04-15 2006-11-16 Henry Adam S Gel cassette adaptor
US7588673B2 (en) * 2005-09-14 2009-09-15 Bio-Rad Laboratories, Inc. Electrophoresis cassette with sealing and closure features
US8262889B2 (en) * 2009-02-20 2012-09-11 Bio-Rad Laboratories, Inc. Unitary electrode and electrode support for horizontal electrophoresis
EP3650697B1 (en) 2013-03-15 2022-12-14 Kaeser Kompressoren Se Measurement value standardisation
GB201315011D0 (en) * 2013-08-22 2013-10-02 Univ Leicester Assay
USD975873S1 (en) 2020-07-13 2023-01-17 Life Technologies Corporation Electrophoresis and electrotransfer device
JP2023534015A (en) 2020-07-13 2023-08-07 ライフ テクノロジーズ コーポレーション Electrophoresis and electrophoretic transfer devices, systems, and methods

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3888758A (en) * 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
US4088561A (en) * 1977-06-27 1978-05-09 The United States Of America As Represented By The United States Department Of Energy Apparatus for electrophoresis separation
GB2263543A (en) * 1992-01-14 1993-07-28 Fotodyne Inc Fluid circulator and temperature regulator
DE4307955A1 (en) * 1993-03-12 1994-09-15 Gerhard Aumeier Electrokinetic device
US5562813A (en) * 1992-11-17 1996-10-08 Ingeny B.V. Two-dimensional electrophoresis apparatus and an electrophoresis unit therefor

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5080769A (en) * 1990-09-12 1992-01-14 Beckman Instruments, Inc. Electrophoresis with electrode baffles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3888758A (en) * 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
US4088561A (en) * 1977-06-27 1978-05-09 The United States Of America As Represented By The United States Department Of Energy Apparatus for electrophoresis separation
GB2263543A (en) * 1992-01-14 1993-07-28 Fotodyne Inc Fluid circulator and temperature regulator
US5562813A (en) * 1992-11-17 1996-10-08 Ingeny B.V. Two-dimensional electrophoresis apparatus and an electrophoresis unit therefor
DE4307955A1 (en) * 1993-03-12 1994-09-15 Gerhard Aumeier Electrokinetic device

Also Published As

Publication number Publication date
EP1315961A2 (en) 2003-06-04
EP1315961B1 (en) 2011-11-02
CA2396970A1 (en) 2002-03-14
CA2396970C (en) 2010-05-11
JP3917515B2 (en) 2007-05-23
DE01968542T1 (en) 2004-07-08
AU769397B2 (en) 2004-01-22
US6451193B1 (en) 2002-09-17
JP2004508549A (en) 2004-03-18
WO2002021116A2 (en) 2002-03-14
AU8878401A (en) 2002-03-22

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