WO2002022857A3 - Methods for the identification of modulators of magnesium chelatase expression or activity in plants - Google Patents

Methods for the identification of modulators of magnesium chelatase expression or activity in plants Download PDF

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Publication number
WO2002022857A3
WO2002022857A3 PCT/US2001/028761 US0128761W WO0222857A3 WO 2002022857 A3 WO2002022857 A3 WO 2002022857A3 US 0128761 W US0128761 W US 0128761W WO 0222857 A3 WO0222857 A3 WO 0222857A3
Authority
WO
WIPO (PCT)
Prior art keywords
chelatase
identification
expression
arabidopsis
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/028761
Other languages
French (fr)
Other versions
WO2002022857A2 (en
WO2002022857A8 (en
Inventor
Jeffrey P Woessner
Adel Zayed
Jorn Gorlach
Douglas Boyes
Keith Davis
Carol Hamilton
Neil Hoffman
Andreas Kloti
Robert Ascenzi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cogenics Icoria Inc
Original Assignee
Paradigm Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc filed Critical Paradigm Genetics Inc
Priority to EP01970987A priority Critical patent/EP1444524A2/en
Priority to AU2001290927A priority patent/AU2001290927A1/en
Priority to PCT/US2001/028761 priority patent/WO2002022857A2/en
Publication of WO2002022857A2 publication Critical patent/WO2002022857A2/en
Anticipated expiration legal-status Critical
Publication of WO2002022857A3 publication Critical patent/WO2002022857A3/en
Publication of WO2002022857A8 publication Critical patent/WO2002022857A8/en
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Biochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present inventors have discovered that Mg-chelatase is essential for the growth of Arabidopsis. Specifically, the inhibition of Mg-chelatase CHL,H gene expression in Arabidopsis seedlings results in varying levels of chlorosis (yellowing), significantly reduced growth and developmental abnormalities. Thus, Arabidopsis Mg-chelatase can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that modulate Arabidopsis Mg-chelatase expression or activity, comprising: contacting a compound with a Arabidopsis Mg-chelatase, or a subunit thereof, and detecting the presence and/or absence of binding between said compound and said Mg-chelatase, or detecting a change in Mg-chelatase expression or activity. The methods of the invention are useful for the identification of herbicides and other compounds that can modulate plant growth and development. In addition, the methods of the invention are useful for the identification of compounds that stimulate the expression or function of Mg-chelatase expression or function. Such compounds can be used to promote or manipulate plant growth and development.
PCT/US2001/028761 2000-09-12 2001-09-14 Methods for the identification of modulators of magnesium chelatase expression or activity in plants Ceased WO2002022857A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01970987A EP1444524A2 (en) 2001-09-14 2001-09-14 Methods for the identification of modulators of magnesium chelatase expression or activity in plants
AU2001290927A AU2001290927A1 (en) 2000-09-12 2001-09-14 Methods for the identification of modulators of magnesium chelatase expression or activity in plants
PCT/US2001/028761 WO2002022857A2 (en) 2000-09-12 2001-09-14 Methods for the identification of modulators of magnesium chelatase expression or activity in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/659,310 2000-09-12
PCT/US2001/028761 WO2002022857A2 (en) 2000-09-12 2001-09-14 Methods for the identification of modulators of magnesium chelatase expression or activity in plants

Publications (3)

Publication Number Publication Date
WO2002022857A2 WO2002022857A2 (en) 2002-03-21
WO2002022857A3 true WO2002022857A3 (en) 2003-06-19
WO2002022857A8 WO2002022857A8 (en) 2004-06-17

Family

ID=32653968

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/028761 Ceased WO2002022857A2 (en) 2000-09-12 2001-09-14 Methods for the identification of modulators of magnesium chelatase expression or activity in plants

Country Status (2)

Country Link
EP (1) EP1444524A2 (en)
WO (1) WO2002022857A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329489B2 (en) 2000-04-14 2008-02-12 Matabolon, Inc. Methods for drug discovery, disease treatment, and diagnosis using metabolomics
US20020009740A1 (en) 2000-04-14 2002-01-24 Rima Kaddurah-Daouk Methods for drug discovery, disease treatment, and diagnosis using metabolomics
WO2008033575A2 (en) 2006-09-15 2008-03-20 Metabolon, Inc. Methods of identifying biochemical pathways

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19717656A1 (en) * 1997-04-25 1998-10-29 Hoechst Schering Agrevo Gmbh DNA sequences coding for the subunit CHLD of plant magnesium chelatases, as well as methods for determining the activity of plant magnesium chelatases
WO2000075340A2 (en) * 1999-06-04 2000-12-14 E.I. Du Pont De Nemours And Company Magnesium chelatase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19717656A1 (en) * 1997-04-25 1998-10-29 Hoechst Schering Agrevo Gmbh DNA sequences coding for the subunit CHLD of plant magnesium chelatases, as well as methods for determining the activity of plant magnesium chelatases
WO2000075340A2 (en) * 1999-06-04 2000-12-14 E.I. Du Pont De Nemours And Company Magnesium chelatase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
WITKOWSKI D A ET AL: "ACCUMULATION OF PHOTODYNAMIC TETRAPYRROLES INDUCED BY ACIFLUORFEN-METHYL", PLANT PHYSIOLOGY (BETHESDA), vol. 87, no. 3, 1988, pages 632 - 637, XP008012184, ISSN: 0032-0889 *
WUEBERT JOACHIM ET AL: "Hydroxybenzoic acid methylesters inhibit magnesium chelatase activity in cress and barley seedlings.", PLANT PHYSIOLOGY AND BIOCHEMISTRY (PARIS), vol. 35, no. 8, 1997, pages 581 - 587, XP008012210, ISSN: 0981-9428 *

Also Published As

Publication number Publication date
WO2002022857A2 (en) 2002-03-21
EP1444524A2 (en) 2004-08-11
WO2002022857A8 (en) 2004-06-17

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