WO2002022869A3 - Directed evolution method - Google Patents

Directed evolution method Download PDF

Info

Publication number
WO2002022869A3
WO2002022869A3 PCT/GB2001/004108 GB0104108W WO0222869A3 WO 2002022869 A3 WO2002022869 A3 WO 2002022869A3 GB 0104108 W GB0104108 W GB 0104108W WO 0222869 A3 WO0222869 A3 WO 0222869A3
Authority
WO
WIPO (PCT)
Prior art keywords
replicase
nucleic acid
pool
selecting
acid member
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2001/004108
Other languages
French (fr)
Other versions
WO2002022869A2 (en
Inventor
Philipp Holliger
Farid Ghadessy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2421963A priority Critical patent/CA2421963C/en
Priority to AU8610301A priority patent/AU8610301A/en
Priority to JP2002527309A priority patent/JP5009481B2/en
Priority to DE60124272T priority patent/DE60124272T2/en
Priority to DK01965462T priority patent/DK1317539T3/en
Priority to EP01965462A priority patent/EP1317539B1/en
Priority to AU2001286103A priority patent/AU2001286103B2/en
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of WO2002022869A2 publication Critical patent/WO2002022869A2/en
Publication of WO2002022869A3 publication Critical patent/WO2002022869A3/en
Priority to US10/387,387 priority patent/US7514210B2/en
Anticipated expiration legal-status Critical
Priority to US12/070,232 priority patent/US20080166772A1/en
Priority to US12/392,412 priority patent/US20090246853A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Ecology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Soil Working Implements (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)

Abstract

We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed.
PCT/GB2001/004108 2000-09-13 2001-09-13 Directed evolution method Ceased WO2002022869A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
AU2001286103A AU2001286103B2 (en) 2000-09-13 2001-09-13 Directed evolution method
JP2002527309A JP5009481B2 (en) 2000-09-13 2001-09-13 Directional evolution method
DE60124272T DE60124272T2 (en) 2000-09-13 2001-09-13 METHOD FOR TARGETED EVOLUTION
DK01965462T DK1317539T3 (en) 2000-09-13 2001-09-13 Approach to Targeted Evolution
EP01965462A EP1317539B1 (en) 2000-09-13 2001-09-13 Directed evolution method
CA2421963A CA2421963C (en) 2000-09-13 2001-09-13 Directed evolution method
AU8610301A AU8610301A (en) 2000-09-13 2001-09-13 Directed evolution method
US10/387,387 US7514210B2 (en) 2000-09-13 2003-03-13 Compartmentalised self replication method for in vitro evolution of molecular libraries
US12/070,232 US20080166772A1 (en) 2000-09-13 2008-02-15 Methods of increasing the concentration of a nucleic acid
US12/392,412 US20090246853A1 (en) 2000-09-13 2009-02-25 Directed evolution method

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GBGB0022458.4A GB0022458D0 (en) 2000-09-13 2000-09-13 Directed evolution method
GB0022458.4 2000-09-13
US28377101P 2001-04-13 2001-04-13
US60/283,771 2001-04-13
US28550101P 2001-04-20 2001-04-20
US60/285,501 2001-04-20

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/387,387 Continuation US7514210B2 (en) 2000-09-13 2003-03-13 Compartmentalised self replication method for in vitro evolution of molecular libraries
US10/387,387 Continuation-In-Part US7514210B2 (en) 2000-09-13 2003-03-13 Compartmentalised self replication method for in vitro evolution of molecular libraries

Publications (2)

Publication Number Publication Date
WO2002022869A2 WO2002022869A2 (en) 2002-03-21
WO2002022869A3 true WO2002022869A3 (en) 2002-10-17

Family

ID=27255886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/004108 Ceased WO2002022869A2 (en) 2000-09-13 2001-09-13 Directed evolution method

Country Status (11)

Country Link
US (3) US7514210B2 (en)
EP (4) EP1806406A3 (en)
JP (2) JP5009481B2 (en)
AT (2) ATE344320T1 (en)
AU (2) AU8610301A (en)
CA (2) CA2585083A1 (en)
DE (2) DE60124272T2 (en)
DK (1) DK1317539T3 (en)
ES (2) ES2274901T3 (en)
GB (1) GB0022458D0 (en)
WO (1) WO2002022869A2 (en)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7323305B2 (en) 2003-01-29 2008-01-29 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8871444B2 (en) 2004-10-08 2014-10-28 Medical Research Council In vitro evolution in microfluidic systems
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US9228229B2 (en) 2010-02-12 2016-01-05 Raindance Technologies, Inc. Digital analyte analysis
US9273308B2 (en) 2006-05-11 2016-03-01 Raindance Technologies, Inc. Selection of compartmentalized screening method
US9328344B2 (en) 2006-01-11 2016-05-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9440232B2 (en) 2007-02-06 2016-09-13 Raindance Technologies, Inc. Manipulation of fluids and reactions in microfluidic systems
US9448172B2 (en) 2003-03-31 2016-09-20 Medical Research Council Selection by compartmentalised screening
US9498759B2 (en) 2004-10-12 2016-11-22 President And Fellows Of Harvard College Compartmentalized screening by microfluidic control
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification

Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE358177T1 (en) 1997-07-07 2007-04-15 Medical Res Council AN IN VITRO SORTING PROCESS
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
FR2843404A1 (en) * 2002-08-07 2004-02-13 Cellectis Testing cleavage activity of endonucleases, useful as reagents in molecular biology and genomic engineering, that generate overhangs, comprises testing their effects on duplex DNA substrates
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
DK2145956T3 (en) * 2003-11-03 2015-09-21 Medical Res Council Polymerase
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
EP1691792A4 (en) * 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND METHODS FOR IN VITRO / I SORTING OF MOLECULAR AND CELLULAR BANKS
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
EP1743938A1 (en) * 2005-07-12 2007-01-17 NascaCell IP GmbH Means and methods for displaying polypeptides on cells
DE102005037349A1 (en) * 2005-08-08 2007-02-15 Geneart Ag Method for the continuous targeted evolution of proteins in vivo
DE102005037351B3 (en) * 2005-08-08 2007-01-11 Geneart Ag In vitro method for directed evolution of proteins, useful e.g. in pharmaceutical development, uses expression system for performing translation, transcription and reverse transcription
AU2007261445B2 (en) 2006-06-19 2013-04-18 The Johns Hopkins University Single-molecule PCR on microparticles in water-in-oil emulsions
WO2008029085A2 (en) 2006-09-06 2008-03-13 Medical Research Council Polymerase
JP5457184B2 (en) 2006-09-14 2014-04-02 ディエヌエイ・ポリメラーゼ・テクノロジー・インコーポレイテッド Use of TAQ polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors
EP2076592B1 (en) 2006-10-23 2017-04-12 Medical Research Council Polymerase
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
EP2118280B1 (en) 2007-02-23 2010-12-01 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CA2706740C (en) 2007-11-27 2017-01-17 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
US8933320B2 (en) * 2008-01-18 2015-01-13 Tenksolar, Inc. Redundant electrical architecture for photovoltaic modules
US8748727B2 (en) * 2008-01-18 2014-06-10 Tenksolar, Inc. Flat-plate photovoltaic module
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
WO2009155464A2 (en) * 2008-06-18 2009-12-23 Life Technologies Corporation Mutated and chemically modified thermally stable dna polymerases
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
CN102245761A (en) 2008-11-03 2011-11-16 卡帕生物系统 modified type A DNA polymerase
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
CN103952482A (en) 2009-04-02 2014-07-30 弗卢伊蒂格姆公司 Multi-primer amplification method for barcoding of target nucleic acids
US8003329B2 (en) * 2009-05-12 2011-08-23 Becton Dickinson & Company Molecular counting by color-coded micelles
IN2012DN00387A (en) * 2009-06-15 2015-08-21 Tenksolar Inc
JP2011051943A (en) * 2009-09-03 2011-03-17 Tosoh Corp Protein extraction reagent and method for screening polymerase therewith
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
WO2011056872A2 (en) * 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP2569441B2 (en) * 2010-05-14 2017-09-13 cSens AB Kit and method for measuring the activity of deoxynucleoside kinase using dna-dependent dna polymerase and natural deoxynucleosides
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
EP2714970B1 (en) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
EP3461807B1 (en) 2011-06-08 2023-07-12 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
EP2742151B1 (en) 2011-08-10 2017-10-25 Life Technologies Corporation Polymerase compositions
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US20130157259A1 (en) * 2011-12-15 2013-06-20 Samsung Electronics Co., Ltd. Method of amplifying dna from rna in sample and use thereof
CN104334739A (en) 2012-01-13 2015-02-04 Data生物有限公司 Genotyping by Next Generation Sequencing
WO2013116698A2 (en) 2012-02-02 2013-08-08 Invenra, Inc. High throughput screen for biologically active polypeptides
CN104471077B (en) 2012-05-21 2017-05-24 富鲁达公司 Single particle analysis of particle populations
US10683537B2 (en) * 2012-10-16 2020-06-16 Dna Polymerase Technology, Inc. Inhibition-resistant polymerases
WO2014065760A1 (en) * 2012-10-25 2014-05-01 Agency For Science, Technology And Research Methods for determining resistance against molecules targeting proteins
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
JP6742238B2 (en) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Novel compounds for use in PCR systems and uses thereof
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015100277A2 (en) * 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
US9976178B2 (en) 2014-12-16 2018-05-22 Life Technologies Corporation Polymerase compositions and methods of making and using same
US11091745B2 (en) 2015-05-12 2021-08-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CA3006994A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
US10323243B2 (en) 2016-01-19 2019-06-18 Board Of Regents, The University Of Texas System Thermostable reverse transcriptase
WO2018031625A2 (en) 2016-08-09 2018-02-15 Integrated Dna Technologies, Inc. Rnase h mutants in an emulsion
WO2018064338A1 (en) 2016-09-29 2018-04-05 Stratos Genomics, Inc. Directed evolution of enzymes by plasmid tagging in droplets
US20180135080A1 (en) * 2016-11-15 2018-05-17 Genomic Vision Sa Method for the monitoring of modified nucleases induced-gene editing events by molecular combing
CN108265039B (en) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 Mutant TaqDNA polymerase and purification method thereof
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
WO2018232282A1 (en) * 2017-06-15 2018-12-20 Board Of Regents, The University Of Texas System Methods and platform for screening and selecting metabolites and their receptors
LT6615B (en) 2017-09-12 2019-04-25 Vilniaus Universitetas N4-modified cytidine nucleotides and their use
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System Tumor specific antibodies and t-cell receptors and methods of identifying the same
WO2019143622A1 (en) 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
LT6742B (en) * 2018-10-10 2020-07-10 Vilnius University Catalytic biomolecule activity recording into dna sequence
CN113383086A (en) * 2019-03-10 2021-09-10 武汉爱博泰克生物科技有限公司 Mutant Taq polymerase for amplification in increased salt concentration or body fluids
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
CN110218776A (en) * 2019-06-14 2019-09-10 苏州叠代生物科技有限公司 PCR oil phase composition and preparation method thereof
WO2021025623A1 (en) * 2019-08-08 2021-02-11 Agency For Science, Technology And Research Method of detecting protein-protein interactions
US12270026B1 (en) 2020-03-05 2025-04-08 Ansa Biotechnologies, Inc. Directed evolution method for template-independent polymerases
CN111621482B (en) * 2020-06-30 2022-04-29 浙江工业大学 A kind of glufosinate-ammonium dehydrogenase mutant, genetic engineering bacteria and one-pot multi-enzyme simultaneous directed evolution method
CN114480327B (en) * 2020-10-26 2024-01-16 厦门大学 Taq DNA polymerase mutant
US12540948B2 (en) 2021-03-05 2026-02-03 Countable Labs, Inc. Systems and methods for generating droplets and performing digital analyses
WO2022187684A1 (en) 2021-03-05 2022-09-09 Enumerix, Inc. Systems and methods for generating droplets and performing digital analyses
CN112695019B (en) * 2021-03-23 2021-06-25 翌圣生物科技（上海）有限公司 Reverse transcriptase mutant and application thereof
US12655479B2 (en) 2021-10-11 2026-06-16 Dna Polymerase Technology, Inc. Fast and robust bi-functional mutants of Taq DNA polymerase
JPWO2024009873A1 (en) * 2022-07-08 2024-01-11
WO2024211850A1 (en) * 2023-04-05 2024-10-10 Primrose Bio, Inc. Methods and compositions for protein engineering
EP4695384A2 (en) * 2023-04-13 2026-02-18 5Prime Biosciences, Inc. Compositions and methods for upregulation of reverse transcription and reduction of sequence bias in rna sequencing
DE102023001827A1 (en) 2023-04-19 2024-10-24 Horst Wochnowski Method and associated device for cultivating microorganism strains that decompose and degrade microplastics in waters by an artificially induced and accelerated evolution and selection process

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994026766A1 (en) * 1993-02-19 1994-11-24 Barnes Wayne M Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension
WO1996040723A1 (en) * 1995-06-07 1996-12-19 The General Hospital Corporation Catalytic dna
WO1997047763A1 (en) * 1996-06-14 1997-12-18 Curagen Corporation Identification and comparison of protein-protein interactions and inhibitors thereof
WO1998013502A2 (en) * 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions
WO1999002671A1 (en) * 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method
WO2000004139A1 (en) * 1998-07-17 2000-01-27 Mirus Corporation Micellar systems
WO2000040712A1 (en) * 1999-01-07 2000-07-13 Medical Research Council Optical sorting method

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290479A (en) 1986-12-09 1994-03-01 Phillips Petroleum Company Surfactant system of polyethoxylated compounds and glyceride compounds
GB9107628D0 (en) * 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
ATE175997T1 (en) * 1991-08-10 1999-02-15 Medical Res Council TREATMENT OF CELL POPULATIONS
GB9818432D0 (en) * 1998-08-24 1998-10-21 Bioline Limited Thermostable DNA Polymerase
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994026766A1 (en) * 1993-02-19 1994-11-24 Barnes Wayne M Dna polymerases with enhanced thermostability and enhanced length and efficiency of primer extension
WO1996040723A1 (en) * 1995-06-07 1996-12-19 The General Hospital Corporation Catalytic dna
WO1997047763A1 (en) * 1996-06-14 1997-12-18 Curagen Corporation Identification and comparison of protein-protein interactions and inhibitors thereof
WO1998013502A2 (en) * 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions
WO1999002671A1 (en) * 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method
WO2000004139A1 (en) * 1998-07-17 2000-01-27 Mirus Corporation Micellar systems
WO2000040712A1 (en) * 1999-01-07 2000-07-13 Medical Research Council Optical sorting method

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
ANARBAEV RASHID O ET AL: "Klenow fragment and DNA polymerase alpha-primase from serva calf thymus in water-in-oil microemulsions.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1384, no. 2, 19 May 1998 (1998-05-19), pages 315 - 324, XP004278360, ISSN: 0006-3002 *
EIGEN M ET AL: "THE HYPERCYCLE. COUPLING OF RNA AND PROTEIN BIOSYNTHESIS IN THE INFECTION CYCLE OF AN RNA BACTERIOPHAGE", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 30, no. 46, 19 November 1991 (1991-11-19), pages 11005 - 11018, XP002065626, ISSN: 0006-2960 *
EIGEN M: "WIE ENTSTEHT INFORMATION? PRINZIPIEN DER SELBSTORGANISATION IN DER BIOLOGIE", BERICHTE DER BUNSEN-GESELLSCHAFT FUR PHYSIKALISCHE CHEMIE, VERLAG CHEMIE. WEINHEIM, DE, vol. 80, no. 11, 1976, pages 1059 - 1081, XP000605250, ISSN: 0005-9021 *
GHADESSY FARID J ET AL: "Directed evolution of polymerase function by compartmentalized self-replication.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 8, 10 April 2001 (2001-04-10), April 10, 2001, pages 4552 - 4557, XP002189673, ISSN: 0027-8424 *
M. EIGEN ET AL.: "Hypercycles and compartments", J. THEORETICAL BIOLOGY, vol. 85, 1980, ACADEMIC PRESS INC., LONDON, UK, pages 407 - 411, XP008004373 *
OBERHOLZER T ET AL: "POLYMERASE CHAIN REACTION IN LIPOSOMES", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 10, no. 2, October 1995 (1995-10-01), pages 677 - 682, XP008004376, ISSN: 1074-5521 *
PELLETIER ET AL: "An in vivo library-versus-library selection of optimized protein-protein interactions", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, July 1999 (1999-07-01), pages 6836 - 90, XP002156488, ISSN: 1087-0156 *
SUZUKI M ET AL: "RANDOM MUTAGENESIS OF THERMUS AQUATICUS DNA POLYMERASE I: CONCORDANCE OF IMMUTABLE SITES IN VIVO WITH THE CRYSTAL STRUCTURE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 93, 1 September 1996 (1996-09-01), pages 9670 - 9675, XP002062865, ISSN: 0027-8424 *
TAWFIK AND A D GRIFFITHS D S: "Man-made cell-like compartments for molecular evolution", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 7, no. 16, 1 July 1998 (1998-07-01), pages 652 - 656, XP002079691, ISSN: 1087-0156 *
VAINSHTEIN INNA ET AL: "Peptide rescue of an N-terminal truncation of the Stoffel fragment of Taq DNA polymerase.", PROTEIN SCIENCE, vol. 5, no. 9, 1996, pages 1785 - 1792, XP001053487, ISSN: 0961-8368 *

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US7323305B2 (en) 2003-01-29 2008-01-29 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US9448172B2 (en) 2003-03-31 2016-09-20 Medical Research Council Selection by compartmentalised screening
US9186643B2 (en) 2004-10-08 2015-11-17 Medical Research Council In vitro evolution in microfluidic systems
US8871444B2 (en) 2004-10-08 2014-10-28 Medical Research Council In vitro evolution in microfluidic systems
US9498759B2 (en) 2004-10-12 2016-11-22 President And Fellows Of Harvard College Compartmentalized screening by microfluidic control
US9534216B2 (en) 2006-01-11 2017-01-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9328344B2 (en) 2006-01-11 2016-05-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9410151B2 (en) 2006-01-11 2016-08-09 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US9273308B2 (en) 2006-05-11 2016-03-01 Raindance Technologies, Inc. Selection of compartmentalized screening method
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9498761B2 (en) 2006-08-07 2016-11-22 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9440232B2 (en) 2007-02-06 2016-09-13 Raindance Technologies, Inc. Manipulation of fluids and reactions in microfluidic systems
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9228229B2 (en) 2010-02-12 2016-01-05 Raindance Technologies, Inc. Digital analyte analysis
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing

Also Published As

Publication number Publication date
DE60124272T2 (en) 2007-05-03
JP2004508834A (en) 2004-03-25
AU2001286103B2 (en) 2006-08-10
EP1317539B1 (en) 2006-11-02
EP1806406A3 (en) 2009-07-29
CA2585083A1 (en) 2002-03-21
US7514210B2 (en) 2009-04-07
EP1505151B1 (en) 2008-05-21
ATE344320T1 (en) 2006-11-15
EP1505151A3 (en) 2005-05-11
EP1505151A2 (en) 2005-02-09
JP2007190027A (en) 2007-08-02
DE60124272D1 (en) 2006-12-14
US20080166772A1 (en) 2008-07-10
US20040005594A1 (en) 2004-01-08
AU8610301A (en) 2002-03-26
EP1317539A2 (en) 2003-06-11
CA2421963A1 (en) 2002-03-21
WO2002022869A2 (en) 2002-03-21
EP1964932A3 (en) 2009-05-20
ES2304580T3 (en) 2008-10-16
ES2274901T3 (en) 2007-06-01
GB0022458D0 (en) 2000-11-01
CA2421963C (en) 2013-04-09
DE60134181D1 (en) 2008-07-03
US20090246853A1 (en) 2009-10-01
JP5009481B2 (en) 2012-08-22
DK1317539T3 (en) 2007-03-05
EP1806406A2 (en) 2007-07-11
EP1964932A2 (en) 2008-09-03
ATE396259T1 (en) 2008-06-15

Similar Documents

Publication Publication Date Title
EP1806406A3 (en) Modified replicase enzymes
WO2002010210A3 (en) Polypeptides for identifying herbicidally active compounds
WO2001057194A3 (en) Nucleic acid molecules encoding transmembrane serine proteases, the encoded proteins and methods based thereon
WO2001075087A3 (en) Subtilisin variants
WO2002031134A3 (en) Novel serine protease genes related to dppiv
WO2002042418A3 (en) 3-hydroxypropionic acid and other organic compounds
WO1998008974A3 (en) Catalytic nucleic acid and its medical use
WO2002103011A3 (en) Selective gene amplification
WO2003097810A3 (en) Nitrilases, nucleic acids encoding them and methods for making and using them
WO2001092308A3 (en) Cystine-knot polypeptides: cloaked-2 molecules and uses thereof
WO2003068961A3 (en) Method to modify differentiation of pluripotential stem cells
WO2000004865A3 (en) Synthetic techniques and intermediates for polyhydroxy, dienyl lactone derivatives
WO2003050260A3 (en) Streptavidin expressed gene fusions and methods of use thereof
WO2001068859A3 (en) Il-17 receptor like molecules and uses thereof
WO2002077263A3 (en) Nucleic acid molecules encoding serine protease cvsp14, the encoded polypeptides and methods based thereon
WO2003048308A3 (en) Thermus brockianus nucleic acid polymerases
MXPA03005492A (en) Method for the concentration of spent acid.
HK1049185A1 (en) Modified fluorescent proteins
WO2002090549A3 (en) Compositions and methods for the modulation of viral maturation
WO2001074858A3 (en) Methods for altering t cell activation
WO2003048309A3 (en) Thermus thermophilus nucleic acid polymerases
WO2003044179A3 (en) Nucleic acid molecules encoding serine protease 17, the encoded polypeptides and methods based thereon
WO2002044361A8 (en) Eukaryotic expression libraries based on double lox recombination and methods of use
WO2001098469A3 (en) Polypeptides having peroxidase activity and nucleic acids encoding same
WO2003048310A3 (en) Thermus oshimai nucleic acid polymerases

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2001965462

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002527309

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2421963

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 10387387

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2001286103

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 2001965462

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 2001965462

Country of ref document: EP