WO2002024890A3 - Rice mlh1 ortholog and uses thereof - Google Patents

Rice mlh1 ortholog and uses thereof Download PDF

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Publication number
WO2002024890A3
WO2002024890A3 PCT/US2001/029088 US0129088W WO0224890A3 WO 2002024890 A3 WO2002024890 A3 WO 2002024890A3 US 0129088 W US0129088 W US 0129088W WO 0224890 A3 WO0224890 A3 WO 0224890A3
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WO
WIPO (PCT)
Prior art keywords
mlh1
plant
polypeptide
mismatch repair
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/029088
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French (fr)
Other versions
WO2002024890A2 (en
Inventor
Pramod B Mahajan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
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Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Priority to EP01971133A priority Critical patent/EP1319081A2/en
Priority to AU2001291057A priority patent/AU2001291057A1/en
Priority to CA002422487A priority patent/CA2422487A1/en
Publication of WO2002024890A2 publication Critical patent/WO2002024890A2/en
Publication of WO2002024890A3 publication Critical patent/WO2002024890A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Cereal-Derived Products (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Compositions and methods for inhibiting the cellular mismatch repair system in a plant host cell are provided. Compositions include the cDNA and amino acid sequence of a rice MLH1 ortholog. The nucleic acid molecules and proteins of the invention find use in increasing the efficiency of targeted gene mutation and homologous recombination in plants via inhibition of the plant cellular mismatch repair system. The plant cellular mismatch repair system is inhibited through the use of transposon tagging of a MLH1 gene, sense- and antisense-suppression of a MLH1 gene, antibody binding to a MLH1 polypeptide or variant polypeptide, targeted mutagenesis of specific amino acid residues of a plant MLH1 gene, and competition with a mismatch repair impaired MLH1 polypeptide through transgeneic over-expression of the impaired polypeptide. Also provided are transformed plant cells, plant tissues, plants, and seeds. Additional methods that are provided include the detection of as little as one base pair mismatch in a DNA duplex and the generation of plants with reversible male sterility for applications in hybrid generation.
PCT/US2001/029088 2000-09-18 2001-09-18 Rice mlh1 ortholog and uses thereof Ceased WO2002024890A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01971133A EP1319081A2 (en) 2000-09-18 2001-09-18 Rice mlh1 ortholog and uses thereof
AU2001291057A AU2001291057A1 (en) 2000-09-18 2001-09-18 Rice MLH1 ortholog and uses thereof
CA002422487A CA2422487A1 (en) 2000-09-18 2001-09-18 Rice mlh1 ortholog and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23312400P 2000-09-18 2000-09-18
US60/233,124 2000-09-18

Publications (2)

Publication Number Publication Date
WO2002024890A2 WO2002024890A2 (en) 2002-03-28
WO2002024890A3 true WO2002024890A3 (en) 2003-01-30

Family

ID=22875973

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/029088 Ceased WO2002024890A2 (en) 2000-09-18 2001-09-18 Rice mlh1 ortholog and uses thereof

Country Status (5)

Country Link
US (1) US20020088021A1 (en)
EP (1) EP1319081A2 (en)
AU (1) AU2001291057A1 (en)
CA (1) CA2422487A1 (en)
WO (1) WO2002024890A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1333095T3 (en) * 2002-01-31 2006-07-17 Mixis France Sa Method for reversible, functional inactivation of the mismatch repair system
US20040002156A1 (en) * 2002-06-26 2004-01-01 Stratagene Selective cloning of homoduplex nucleic acids
WO2004070035A2 (en) * 2003-02-03 2004-08-19 Sungene Gmbh & Co. Kgaa Method for increasing efficiency of homologous recombination in plants
US20090031444A1 (en) * 2005-09-09 2009-01-29 Peter Egbertus Wittich Homologous recombination in plants
US20090068741A1 (en) * 2007-01-17 2009-03-12 Invitrogen Corporation Methods and Compositions for Improving the Health of Cells in Culture
CN104975022B (en) * 2014-04-08 2019-05-21 未名兴旺系统作物设计前沿实验室（北京）有限公司 The identification and application of plant anther specific expression promoter pTaASG036
CN104542299B (en) * 2015-01-26 2016-03-16 云南省农业科学院生物技术与种质资源研究所 A kind of method improving common wild-rice filial generation Anther Culture Efficiency
CN114685634A (en) * 2020-12-30 2022-07-01 中国科学院分子植物科学卓越创新中心 Gene for regulating and controlling seed setting rate and application thereof

Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007576A1 (en) * 1988-12-26 1990-07-12 Setratech In vivo recombination process for partially homologous dna sequences
WO1993022462A1 (en) * 1992-05-06 1993-11-11 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
WO1996017945A1 (en) * 1994-12-08 1996-06-13 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
WO1997021837A1 (en) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methods for the detection and removal of mutant sequences that arise during enzymatic amplification using mismatch repair systems
WO1997037011A1 (en) * 1996-04-01 1997-10-09 Setratech S.A.R.L. Meiotic recombination in vivo of partially homologous dna sequences
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
WO1998053099A1 (en) * 1997-05-20 1998-11-26 Genolife Method for detecting qualitative and quantitative alterations in dna and ligands of said alteration ligands
WO1999019492A2 (en) * 1997-10-10 1999-04-22 Aventis Cropscience S.A. Methods for obtaining plant varieties
WO1999042622A1 (en) * 1998-02-23 1999-08-26 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compound and uses thereof_____________________________________
CA2240609A1 (en) * 1998-04-14 1999-10-14 The Johns Hopkins University A method for generating hypermutable organisms
WO2000004190A1 (en) * 1998-07-15 2000-01-27 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO2000009723A1 (en) * 1998-08-14 2000-02-24 Pioneer Hi-Bred International, Inc. Maize rad6 genes and uses thereof
WO2000009727A2 (en) * 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
WO2000036109A1 (en) * 1998-12-15 2000-06-22 Pioneer Hi-Bred International, Inc. Maize rad2/fen-1 orthologues and uses thereof
WO2001061012A1 (en) * 2000-02-18 2001-08-23 Nicolaides Nicholas C A method for generating hypermutable plants

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007576A1 (en) * 1988-12-26 1990-07-12 Setratech In vivo recombination process for partially homologous dna sequences
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
WO1993022462A1 (en) * 1992-05-06 1993-11-11 The Board Of Trustees Of The Leland Stanford Junior University Genomic mismatch scanning
WO1996017945A1 (en) * 1994-12-08 1996-06-13 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
WO1997005268A1 (en) * 1995-07-26 1997-02-13 Setratech Homologous recombination in mismatch repair inactivated eukaryotic cells
WO1997021837A1 (en) * 1995-12-15 1997-06-19 Amersham Life Science, Inc. Methods for the detection and removal of mutant sequences that arise during enzymatic amplification using mismatch repair systems
WO1997037011A1 (en) * 1996-04-01 1997-10-09 Setratech S.A.R.L. Meiotic recombination in vivo of partially homologous dna sequences
WO1998053099A1 (en) * 1997-05-20 1998-11-26 Genolife Method for detecting qualitative and quantitative alterations in dna and ligands of said alteration ligands
WO1999019492A2 (en) * 1997-10-10 1999-04-22 Aventis Cropscience S.A. Methods for obtaining plant varieties
WO1999042622A1 (en) * 1998-02-23 1999-08-26 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compound and uses thereof_____________________________________
CA2240609A1 (en) * 1998-04-14 1999-10-14 The Johns Hopkins University A method for generating hypermutable organisms
WO2000004190A1 (en) * 1998-07-15 2000-01-27 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
WO2000009727A2 (en) * 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling to produce herbicide selective crops
WO2000009723A1 (en) * 1998-08-14 2000-02-24 Pioneer Hi-Bred International, Inc. Maize rad6 genes and uses thereof
WO2000036109A1 (en) * 1998-12-15 2000-06-22 Pioneer Hi-Bred International, Inc. Maize rad2/fen-1 orthologues and uses thereof
WO2001061012A1 (en) * 2000-02-18 2001-08-23 Nicolaides Nicholas C A method for generating hypermutable plants

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CULLIGAN AND J B HAYS K M: "DNA mismatch repair in plants", PLANT PHYSIOLOGY, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 115, 1997, pages 833 - 839, XP002099372, ISSN: 0032-0889 *
DATABASE EMBL [online] 18 November 1998 (1998-11-18), KUNZE R.: "Arabidopsis thaliana mRNA for MLH1 protein, partial", XP002205522, Database accession no. AJ012747 *
DATABASE EMBL [online] 22 February 2001 (2001-02-22), SASAKI T., ET AL.: "Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0401G10.", XP002205525, Database accession no. AP003238 *
DATABASE EMBL [online] 24 January 2001 (2001-01-24), SASAKI T., ET AL.: "Oryza sativa (japonica cultivar-group) cDNA, partial sequence (C30062_98A).", XP002205524, Database accession no. AU166528 *
DATABASE EMBL [online] 7 June 1999 (1999-06-07), SASAKI T.: "Oryza sativa (japonica cultivar-group) cDNA, partial sequence (C30062_1Z).", XP002205523, Database accession no. AU068376 *
EDELMANN W ET AL: "Meiotic pachytene arrest in MLH1-deficient mice", CELL, CELL PRESS, CAMBRIDGE, NA, US, vol. 85, no. 7, 28 June 1196 (1196-06-28), pages 1125 - 1134, XP002138359, ISSN: 0092-8674 *
JEAN M ET AL: "Isolation and characterization of AtMLH1, a MutL homologue from Arabidopsis thaliana.", MOLECULAR AND GENERAL GENETICS, vol. 262, no. 4-5, aj012747, December 1999 (1999-12-01), pages 633 - 642, XP000986138, ISSN: 0026-8925 *
NICOLAIDES NICHOLAS C ET AL: "A naturally occurring hPMS2 mutation can confer a dominant negative mutator phenotype", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 18, no. 3, March 1998 (1998-03-01), pages 1635 - 1641, XP002165242, ISSN: 0270-7306 *

Also Published As

Publication number Publication date
US20020088021A1 (en) 2002-07-04
WO2002024890A2 (en) 2002-03-28
AU2001291057A1 (en) 2002-04-02
EP1319081A2 (en) 2003-06-18
CA2422487A1 (en) 2002-03-28

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