WO2002031484A3 - Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry - Google Patents

Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry Download PDF

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Publication number
WO2002031484A3
WO2002031484A3 PCT/US2000/028261 US0028261W WO0231484A3 WO 2002031484 A3 WO2002031484 A3 WO 2002031484A3 US 0028261 W US0028261 W US 0028261W WO 0231484 A3 WO0231484 A3 WO 0231484A3
Authority
WO
WIPO (PCT)
Prior art keywords
tandem mass
molecular interactions
affinity capture
methods
mass spectrometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/028261
Other languages
French (fr)
Other versions
WO2002031484A8 (en
WO2002031484A2 (en
Inventor
Scot Weinberger
Tina Morris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Human Genome Sciences Inc
Aspira Womens Health Inc
Original Assignee
Human Genome Sciences Inc
Ciphergen Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Human Genome Sciences Inc, Ciphergen Biosystems Inc filed Critical Human Genome Sciences Inc
Priority to PCT/US2000/028261 priority Critical patent/WO2002031484A2/en
Priority to CA002425434A priority patent/CA2425434A1/en
Priority to EP00973492A priority patent/EP1327151A2/en
Priority to AU1199501A priority patent/AU1199501A/en
Priority to CNA008199639A priority patent/CN1474944A/en
Priority to JP2002534819A priority patent/JP2004511773A/en
Publication of WO2002031484A2 publication Critical patent/WO2002031484A2/en
Publication of WO2002031484A3 publication Critical patent/WO2002031484A3/en
Priority to US10/406,023 priority patent/US20030219731A1/en
Anticipated expiration legal-status Critical
Publication of WO2002031484A8 publication Critical patent/WO2002031484A8/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
    • H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Plasma & Fusion (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention provides an analytical instrument comprising an affinity capture probe interface, a laser desorption ionization source, and a tandem mass spectrometer. Also presented are new methods for protein discovery and identification and for characterization of molecular interactions that utilize the instrument of the present invention.
PCT/US2000/028261 2000-10-11 2000-10-11 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry Ceased WO2002031484A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
PCT/US2000/028261 WO2002031484A2 (en) 2000-10-11 2000-10-11 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry
CA002425434A CA2425434A1 (en) 2000-10-11 2000-10-11 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry
EP00973492A EP1327151A2 (en) 2000-10-11 2000-10-11 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry
JP2002534819A JP2004511773A (en) 2000-10-11 2000-10-12 A method for characterizing molecular interactions using affinity capture tandem mass spectrometry
CNA008199639A CN1474944A (en) 2000-10-11 2000-10-12 Method for characterizing intermolecular interactions by using affinity capture tandem mass spectrometry
AU1199501A AU1199501A (en) 2000-10-11 2000-10-12 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry
US10/406,023 US20030219731A1 (en) 2000-10-11 2003-04-01 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/028261 WO2002031484A2 (en) 2000-10-11 2000-10-11 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/406,023 Continuation US20030219731A1 (en) 2000-10-11 2003-04-01 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry

Publications (3)

Publication Number Publication Date
WO2002031484A2 WO2002031484A2 (en) 2002-04-18
WO2002031484A3 true WO2002031484A3 (en) 2002-07-25
WO2002031484A8 WO2002031484A8 (en) 2003-09-04

Family

ID=21741885

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/028261 Ceased WO2002031484A2 (en) 2000-10-11 2000-10-11 Methods for characterizing molecular interactions using affinity capture tandem mass spectrometry

Country Status (7)

Country Link
US (1) US20030219731A1 (en)
EP (1) EP1327151A2 (en)
JP (1) JP2004511773A (en)
CN (1) CN1474944A (en)
AU (1) AU1199501A (en)
CA (1) CA2425434A1 (en)
WO (1) WO2002031484A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0101555D0 (en) * 2001-05-04 2001-05-04 Amersham Pharm Biotech Ab Fast variable gain detector system and method of controlling the same
US6946653B2 (en) * 2001-11-27 2005-09-20 Ciphergen Biosystems, Inc. Methods and apparatus for improved laser desorption ionization tandem mass spectrometry
US7846748B2 (en) * 2002-12-02 2010-12-07 The University Of North Carolina At Chapel Hill Methods of quantitation and identification of peptides and proteins
CN1314968C (en) * 2004-03-11 2007-05-09 复旦大学 Trace polypeptide or protein-enriched and its direct analyzing method
CN100376895C (en) * 2004-11-03 2008-03-26 中国科学院计算技术研究所 A method for identifying peptides using tandem mass spectrometry data
CN100483394C (en) * 2006-04-14 2009-04-29 中国科学院计算技术研究所 Noise baseline identification method in mass spectrum data processing
US7879799B2 (en) * 2006-08-10 2011-02-01 Institute For Systems Biology Methods for characterizing glycoproteins and generating antibodies for same
WO2012092281A2 (en) * 2010-12-28 2012-07-05 Quest Diagnostics Investments Incorporated Quantitation of insulin by mass spectrometry
GB201110272D0 (en) 2011-06-17 2011-08-03 Isis Innovation Metod of detection by mass spectrometry
CN203367223U (en) * 2012-05-21 2013-12-25 核工业北京地质研究院 Thermal ionization time-of-flight mass spectrometer
CN106415260B (en) * 2014-06-13 2021-04-06 Dh科技发展私人贸易有限公司 Methods of analyzing lipids using mass spectrometry
US10008376B2 (en) * 2014-10-30 2018-06-26 Dh Technologies Development Pte. Ltd. Methods and systems for selecting ions for ion fragmentation
US10049868B2 (en) * 2016-12-06 2018-08-14 Rapiscan Systems, Inc. Apparatus for detecting constituents in a sample and method of using the same
CN114383984B (en) * 2021-12-06 2022-09-23 浙江大学 System for capturing particulate matter and measuring phase state, morphology and chemical components of particulate matter

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994028418A1 (en) * 1993-05-28 1994-12-08 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US5538897A (en) * 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
WO1998059360A1 (en) * 1997-06-20 1998-12-30 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
WO1999040610A2 (en) * 1998-02-06 1999-08-12 Perseptive Biosystems, Inc. A tandem time-of-flight mass spectrometer with delayed extraction and method for use

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US5288644A (en) * 1990-04-04 1994-02-22 The Rockefeller University Instrument and method for the sequencing of genome
US5202563A (en) * 1991-05-16 1993-04-13 The Johns Hopkins University Tandem time-of-flight mass spectrometer
US5144127A (en) * 1991-08-02 1992-09-01 Williams Evan R Surface induced dissociation with reflectron time-of-flight mass spectrometry
US5382793A (en) * 1992-03-06 1995-01-17 Hewlett-Packard Company Laser desorption ionization mass monitor (LDIM)
US6020208A (en) * 1994-05-27 2000-02-01 Baylor College Of Medicine Systems for surface-enhanced affinity capture for desorption and detection of analytes
US5498545A (en) * 1994-07-21 1996-03-12 Vestal; Marvin L. Mass spectrometer system and method for matrix-assisted laser desorption measurements
US5777324A (en) * 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis
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US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994028418A1 (en) * 1993-05-28 1994-12-08 Baylor College Of Medicine Method and apparatus for desorption and ionization of analytes
US5538897A (en) * 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
WO1998059360A1 (en) * 1997-06-20 1998-12-30 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
WO1998059362A1 (en) * 1997-06-20 1998-12-30 Ciphergen Biosystems, Inc. Retentate chromatography and protein chip arrays with applications in biology and medicine
WO1999040610A2 (en) * 1998-02-06 1999-08-12 Perseptive Biosystems, Inc. A tandem time-of-flight mass spectrometer with delayed extraction and method for use

Non-Patent Citations (3)

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ANDERSEN J S ET AL: "Functional genomics by mass spectrometry", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 480, no. 1, 25 August 2000 (2000-08-25), pages 25 - 31, XP004337489, ISSN: 0014-5793 *
PURCELL A W ET AL: "The use of post-source decay in matrix-assisted laser desorption/ionisation mass spectrometry to delineate T cell determinants", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 249, no. 1-2, 1 March 2001 (2001-03-01), pages 17 - 31, XP004317467, ISSN: 0022-1759 *
RAYMACKERS JOS ET AL: "Identification of two-dimensionally separated human cerebrospinal fluid proteins by N-terminal sequencing, matrix-assisted laser desorption/ionization - mass spectrometry, nanoliquid chromatography-electrospray ionization-time of flight-mass spectrometry, and tandem mass spectrometry.", ELECTROPHORESIS, vol. 21, no. 11, June 2000 (2000-06-01), pages 2266 - 2283, XP008001622, ISSN: 0173-0835 *

Also Published As

Publication number Publication date
CN1474944A (en) 2004-02-11
WO2002031484A8 (en) 2003-09-04
CA2425434A1 (en) 2002-04-18
EP1327151A2 (en) 2003-07-16
WO2002031484A2 (en) 2002-04-18
AU1199501A (en) 2002-04-22
US20030219731A1 (en) 2003-11-27
JP2004511773A (en) 2004-04-15

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