WO2002037093A3 - Gel trap for electrophoresis - Google Patents

Gel trap for electrophoresis Download PDF

Info

Publication number
WO2002037093A3
WO2002037093A3 PCT/IL2001/001000 IL0101000W WO0237093A3 WO 2002037093 A3 WO2002037093 A3 WO 2002037093A3 IL 0101000 W IL0101000 W IL 0101000W WO 0237093 A3 WO0237093 A3 WO 0237093A3
Authority
WO
WIPO (PCT)
Prior art keywords
gel matrix
gel
electrophoresis
matrix
communication
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2001/001000
Other languages
French (fr)
Other versions
WO2002037093A2 (en
Inventor
Yitzhak Ben-Asouli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Bio Application Ltd
Original Assignee
Gene Bio Application Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from IL13944700A external-priority patent/IL139447A0/en
Priority claimed from IL13944600A external-priority patent/IL139446A0/en
Application filed by Gene Bio Application Ltd filed Critical Gene Bio Application Ltd
Priority to AU2002214219A priority Critical patent/AU2002214219B2/en
Priority to IL15513801A priority patent/IL155138A0/en
Priority to AU1421902A priority patent/AU1421902A/en
Priority to CA002427022A priority patent/CA2427022A1/en
Priority to EP01982678A priority patent/EP1330644A2/en
Publication of WO2002037093A2 publication Critical patent/WO2002037093A2/en
Publication of WO2002037093A3 publication Critical patent/WO2002037093A3/en
Priority to IL155138A priority patent/IL155138A/en
Priority to US10/422,062 priority patent/US7264703B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Electrostatic Separation (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Jellies, Jams, And Syrups (AREA)

Abstract

The present invention is directed to an apparatus for electrophoresis having a first gel matrix, adapted for performing an electrophoretic process therein, in communication with a second gel matrix, both being accommodated within a suitable housing. The housing has a first opening adapted to permit ionic communication between the first gel matrix and an external ionic buffer solution, and a second opening adapted to permit ionic communication between the second gel matrix and an external ionic buffer solution. The second gel has at least one suitable absorption material capable of retaining therein at least one target substance capable of migrating thereto from the first gel matrix when an electrophoretic process is performed in the first matrix.
PCT/IL2001/001000 2000-11-02 2001-10-29 Gel trap for electrophoresis Ceased WO2002037093A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2002214219A AU2002214219B2 (en) 2000-11-02 2001-10-29 Gel trap for electrophoresis
IL15513801A IL155138A0 (en) 2000-11-02 2001-10-29 Gel trap for electrophoresis
AU1421902A AU1421902A (en) 2000-11-02 2001-10-29 Gel trap for electrophoresis
CA002427022A CA2427022A1 (en) 2000-11-02 2001-10-29 Gel trap for electrophoresis
EP01982678A EP1330644A2 (en) 2000-11-02 2001-10-29 Gel trap for electrophoresis
IL155138A IL155138A (en) 2000-11-02 2003-03-27 Gel trap for electrophoresis
US10/422,062 US7264703B2 (en) 2000-11-02 2003-04-23 Gel trap for electrophoresis

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
IL139446 2000-11-02
IL139447 2000-11-02
IL13944600A IL139446A0 (en) 2000-11-02 2000-11-02 Gel for electrophoresis
IL13944700A IL139447A0 (en) 2000-11-02 2000-11-02 Gel trap for electrophoresis

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/422,062 Continuation US7264703B2 (en) 2000-11-02 2003-04-23 Gel trap for electrophoresis

Publications (2)

Publication Number Publication Date
WO2002037093A2 WO2002037093A2 (en) 2002-05-10
WO2002037093A3 true WO2002037093A3 (en) 2002-07-25

Family

ID=26323988

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/IL2001/001000 Ceased WO2002037093A2 (en) 2000-11-02 2001-10-29 Gel trap for electrophoresis
PCT/IL2001/001001 Ceased WO2002037094A2 (en) 2000-11-02 2001-10-29 Gel for electrophoresis

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/001001 Ceased WO2002037094A2 (en) 2000-11-02 2001-10-29 Gel for electrophoresis

Country Status (9)

Country Link
US (3) US20040020775A1 (en)
EP (2) EP1330643B1 (en)
AT (1) ATE372514T1 (en)
AU (4) AU2002212670B2 (en)
CA (2) CA2427022A1 (en)
DE (1) DE60130353T2 (en)
ES (1) ES2292633T3 (en)
IL (1) IL155138A (en)
WO (2) WO2002037093A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100351831B1 (en) * 2000-10-02 2002-09-11 엘지전자 주식회사 VSB transmission system
IL166716A0 (en) * 2005-02-07 2006-01-15 Gene Bio Applic Ltd Double chamber tank for electrophoresis
USD563562S1 (en) * 2005-02-15 2008-03-04 Serenex, Inc. Gel box for an auto sample feed gel system
HK1206430A1 (en) * 2012-05-31 2016-01-08 Ge Healthcare Bio-Sciences Ab An electrophoresis system and a separation and identification method
CN104364640B (en) * 2012-05-31 2016-10-26 通用电气健康护理生物科学股份公司 The method manufacturing electrophoresis cartridge
WO2014150275A1 (en) * 2013-03-15 2014-09-25 Bio-Rad Laboratories, Inc. Polyacrylamide gels for rapid casting, blotting, and imaging, with storage stability
WO2015106356A1 (en) * 2014-01-17 2015-07-23 Coastal Genomics Inc. Cassettes for use in automated parallel electrophoretic assays and methods for manufacturing and using same
WO2016187535A2 (en) * 2015-05-20 2016-11-24 Zephyrus Biosciences, Inc. System and methods for electrophoretic separation and analysis of analytes
WO2021030427A2 (en) * 2019-08-12 2021-02-18 Yale University Intragel well sample loading system
EP4419901A4 (en) * 2021-10-21 2025-11-19 Zadeh Cameron O HIGH-THROUGH ELECTROPHORETIC GEL DEVICE WITH MULTIPLEXING AND ASSOCIATED PROCEDURES

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3873433A (en) * 1973-02-12 1975-03-25 Immuno Ag Method of demonstrating normal and pathological plasm-lipoprotein patterns in human body liquids
EP0471949A1 (en) * 1990-08-21 1992-02-26 Hewlett-Packard Company Situ sample pretreatment in electrophoresis capillary
WO1992017259A1 (en) * 1991-03-28 1992-10-15 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
US5228971A (en) * 1990-12-20 1993-07-20 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
WO1998010277A1 (en) * 1996-09-06 1998-03-12 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US5827418A (en) * 1996-10-11 1998-10-27 Hoefer Pharmacia Biotech, Inc. Electrophoresis cassette
WO1999030145A1 (en) * 1997-12-05 1999-06-17 Mosaic Technologies Slotted electrophoresis gel composition and methods of use thereof

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL151506B (en) * 1971-08-12 1976-11-15 Biotest Serum Institut Gmbh METHOD AND DEVICE FOR THE IMPLEMENTATION OF A TWO-DIMENSIONAL IMMUNE-ELECTROPHONE.
US3888759A (en) * 1973-05-25 1975-06-10 Yeda Res & Dev Flat plate electrophoresis
DE2406959C3 (en) * 1974-02-14 1979-02-08 Behringwerke Ag, 3550 Marburg Arrangement and procedure for the determination of antigens
DE3232685C2 (en) * 1982-09-02 1985-02-14 Reiner Dr. 8039 Puchheim Westermeier Gel matrix for horizontal one- and / or two-dimensional electrophoretic separation of anions and cations, method for their production and use of a syringe system for sample application for the gel matrix
FR2533704B1 (en) * 1982-09-27 1986-03-21 Spiral IMMUNOLOGICAL ASSAY IN THE SERUM OF APOLIPOPROTEIN B OF LOW DENSITY LIPOPROTEINS
US4666581A (en) * 1984-05-09 1987-05-19 Hitachi, Ltd. Apparatus for two-dimensional electrophoresis
US4608146A (en) * 1985-04-09 1986-08-26 Bio-Rad Laboratories, Inc. Horizontal electrophoresis cell for rapid assembly
US4735697A (en) * 1985-04-17 1988-04-05 Phoresis Transfer Systems, Inc. Method and apparatus for separating complex mixtures of bio-organic materials
US4709810A (en) * 1986-11-14 1987-12-01 Helena Laboratories Corporation Container for an electrophoretic support medium
US4830725A (en) * 1987-08-04 1989-05-16 Life Technologies, Inc. Electrophoresis apparatus
US5443704A (en) * 1991-12-31 1995-08-22 Fmc Corporation Electrophoresis gel container assemblies
WO1995014921A1 (en) * 1993-11-23 1995-06-01 Perkin-Elmer Corporation Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
IL112147A (en) * 1994-01-19 1999-12-22 Du Pont Sample holder and method for automated electrophoresis
US5582702A (en) * 1995-04-26 1996-12-10 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
US5800691A (en) * 1996-12-23 1998-09-01 Guest Elchrom Scientific Ag Electrophoresis gels containing sample wells with enlarged loading area
DE19830988A1 (en) * 1998-07-10 2000-01-20 Lion Bioscience Ag Method for feeding sample material, sample feed part and electrophoresis device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3873433A (en) * 1973-02-12 1975-03-25 Immuno Ag Method of demonstrating normal and pathological plasm-lipoprotein patterns in human body liquids
EP0471949A1 (en) * 1990-08-21 1992-02-26 Hewlett-Packard Company Situ sample pretreatment in electrophoresis capillary
US5228971A (en) * 1990-12-20 1993-07-20 Wisconsin Alumni Research Foundation Horizontal gel electrophoresis apparatus
WO1992017259A1 (en) * 1991-03-28 1992-10-15 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
WO1998010277A1 (en) * 1996-09-06 1998-03-12 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US5827418A (en) * 1996-10-11 1998-10-27 Hoefer Pharmacia Biotech, Inc. Electrophoresis cassette
WO1999030145A1 (en) * 1997-12-05 1999-06-17 Mosaic Technologies Slotted electrophoresis gel composition and methods of use thereof

Also Published As

Publication number Publication date
CA2427025C (en) 2011-09-20
AU2002212670B2 (en) 2006-08-17
US7320747B2 (en) 2008-01-22
ATE372514T1 (en) 2007-09-15
DE60130353D1 (en) 2007-10-18
US20040020776A1 (en) 2004-02-05
CA2427022A1 (en) 2002-05-10
AU1421902A (en) 2002-05-15
WO2002037094A2 (en) 2002-05-10
AU2002214219B2 (en) 2006-08-17
EP1330643B1 (en) 2007-09-05
DE60130353T2 (en) 2008-05-29
IL155138A (en) 2007-06-03
ES2292633T3 (en) 2008-03-16
US20040020775A1 (en) 2004-02-05
WO2002037094A3 (en) 2002-07-25
EP1330644A2 (en) 2003-07-30
AU1267002A (en) 2002-05-15
CA2427025A1 (en) 2002-05-10
EP1330643A2 (en) 2003-07-30
US7264703B2 (en) 2007-09-04
WO2002037093A2 (en) 2002-05-10
US20060207882A1 (en) 2006-09-21

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