WO2002046476A3 - Genotyping using partially-complementary probes - Google Patents

Genotyping using partially-complementary probes Download PDF

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Publication number
WO2002046476A3
WO2002046476A3 PCT/US2001/050997 US0150997W WO0246476A3 WO 2002046476 A3 WO2002046476 A3 WO 2002046476A3 US 0150997 W US0150997 W US 0150997W WO 0246476 A3 WO0246476 A3 WO 0246476A3
Authority
WO
WIPO (PCT)
Prior art keywords
target sequences
genotyping
partially
methods
probes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/050997
Other languages
French (fr)
Other versions
WO2002046476A2 (en
Inventor
Natalie A Solomon
Dwight D Erickson
Sharon R Zeigler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Priority to CA002426346A priority Critical patent/CA2426346A1/en
Priority to EP01988454A priority patent/EP1330549A2/en
Priority to JP2002548192A priority patent/JP2004523221A/en
Publication of WO2002046476A2 publication Critical patent/WO2002046476A2/en
Anticipated expiration legal-status Critical
Publication of WO2002046476A3 publication Critical patent/WO2002046476A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods for detecting at least two distinct target sequences in a test sample are provided. The methods employ probes that are not completely complementary to one another (e.g. SNP), but nevertheless hybridize to each other, to detect the distinct target sequences. The target sequences can be amplified prior to detection with the probes. Variant forms of a single sequence are particularly suited for detection according the methods provided.
PCT/US2001/050997 2000-10-27 2001-10-26 Genotyping using partially-complementary probes Ceased WO2002046476A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002426346A CA2426346A1 (en) 2000-10-27 2001-10-26 Genotyping using partially-complementary probes
EP01988454A EP1330549A2 (en) 2000-10-27 2001-10-26 Genotyping using partially-complementary probes
JP2002548192A JP2004523221A (en) 2000-10-27 2001-10-26 Genotyping using partially complementary probes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69838700A 2000-10-27 2000-10-27
US09/698,387 2000-10-27

Publications (2)

Publication Number Publication Date
WO2002046476A2 WO2002046476A2 (en) 2002-06-13
WO2002046476A3 true WO2002046476A3 (en) 2003-05-01

Family

ID=24805023

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/050997 Ceased WO2002046476A2 (en) 2000-10-27 2001-10-26 Genotyping using partially-complementary probes

Country Status (4)

Country Link
EP (1) EP1330549A2 (en)
JP (1) JP2004523221A (en)
CA (1) CA2426346A1 (en)
WO (1) WO2002046476A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2866349B1 (en) * 2004-02-12 2008-05-16 Biomerieux Sa METHOD FOR DIAGNOSING / PROGNOSING THROMBOSIS

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021938A1 (en) * 1994-02-14 1995-08-17 Rijks Universiteit Leiden A method for screening for the presence of a genetic defect associated with thrombosis and/or poor anticoagulant response to activated protein c
WO1998048052A2 (en) * 1997-04-18 1998-10-29 Abbott Laboratories Amplification based mutation detection
WO1999050454A2 (en) * 1998-04-01 1999-10-07 Whitehead Institute For Biomedical Research Coding sequence polymorphisms in vascular pathology genes
WO1999055916A1 (en) * 1998-04-29 1999-11-04 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
WO2001042512A2 (en) * 1999-11-24 2001-06-14 Incyte Genomics, Inc. Normalization controls and duplex probes for quantitative hybridization reactions

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021938A1 (en) * 1994-02-14 1995-08-17 Rijks Universiteit Leiden A method for screening for the presence of a genetic defect associated with thrombosis and/or poor anticoagulant response to activated protein c
WO1998048052A2 (en) * 1997-04-18 1998-10-29 Abbott Laboratories Amplification based mutation detection
WO1999050454A2 (en) * 1998-04-01 1999-10-07 Whitehead Institute For Biomedical Research Coding sequence polymorphisms in vascular pathology genes
WO1999055916A1 (en) * 1998-04-29 1999-11-04 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
WO2001042512A2 (en) * 1999-11-24 2001-06-14 Incyte Genomics, Inc. Normalization controls and duplex probes for quantitative hybridization reactions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SEVALL J SANDERS: "Factor V Leiden genotyping using real-time fluorescent polymerase chain reaction.", MOLECULAR AND CELLULAR PROBES, vol. 14, no. 4, August 2000 (2000-08-01), pages 249 - 253, XP009003590, ISSN: 0890-8508 *
VON AHSEN NICOLAS ET AL: "Rapid detection of prothrombotic mutations of prothrombin (G20210A), factor V (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 45, no. 5, May 1999 (1999-05-01), pages 694 - 696, XP002152589, ISSN: 0009-9147 *

Also Published As

Publication number Publication date
JP2004523221A (en) 2004-08-05
CA2426346A1 (en) 2002-06-13
EP1330549A2 (en) 2003-07-30
WO2002046476A2 (en) 2002-06-13

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