WO2002048401A3 - Nested oligonucleotides containing hairpin for nucleic acid amplification - Google Patents

Nested oligonucleotides containing hairpin for nucleic acid amplification Download PDF

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Publication number
WO2002048401A3
WO2002048401A3 PCT/US2001/047727 US0147727W WO0248401A3 WO 2002048401 A3 WO2002048401 A3 WO 2002048401A3 US 0147727 W US0147727 W US 0147727W WO 0248401 A3 WO0248401 A3 WO 0248401A3
Authority
WO
WIPO (PCT)
Prior art keywords
engineered
nucleic acid
acid amplification
oligonucleotides containing
containing hairpin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/047727
Other languages
French (fr)
Other versions
WO2002048401A2 (en
Inventor
Katherine S Bowdish
Shana Barbas-Frederickson
Ying-Shi Lin
John Mcwhirter
Toshiaki Maruyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alexion Pharmaceuticals Inc
Original Assignee
Alexion Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharmaceuticals Inc filed Critical Alexion Pharmaceuticals Inc
Priority to CA002436693A priority Critical patent/CA2436693A1/en
Priority to AU2002230734A priority patent/AU2002230734A1/en
Priority to EP01990978A priority patent/EP1366191A2/en
Publication of WO2002048401A2 publication Critical patent/WO2002048401A2/en
Anticipated expiration legal-status Critical
Publication of WO2002048401A3 publication Critical patent/WO2002048401A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Templates that are engineered to contain a predetermined sequence and a hairpin structure are provided by a nested oligonucleotide extension reaction. The engineered template allows Single Primer Amplification (SPA) to amplify a target sequence within the engineered template. In particularly useful embodiments, the target sequences from the engineered templates are cloned into expression vehicles to provide a library a polypeptides or proteins, such as, for example, an antibody library.
PCT/US2001/047727 2000-12-11 2001-12-10 Nested oligonucleotides containing hairpin for nucleic acid amplification Ceased WO2002048401A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002436693A CA2436693A1 (en) 2000-12-11 2001-12-10 Nested oligonucleotides containing hairpin for nucleic acid amplification
AU2002230734A AU2002230734A1 (en) 2000-12-11 2001-12-10 Nested oligonucleotides containing hairpin for nucleic acid amplification
EP01990978A EP1366191A2 (en) 2000-12-11 2001-12-10 Nested oligonucleotides containing hairpin for nucleic acid amplification

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US25466900P 2000-12-11 2000-12-11
US60/254,669 2000-12-11
US32340001P 2001-09-19 2001-09-19
US60/323,400 2001-09-19

Publications (2)

Publication Number Publication Date
WO2002048401A2 WO2002048401A2 (en) 2002-06-20
WO2002048401A3 true WO2002048401A3 (en) 2003-09-12

Family

ID=26944185

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/047727 Ceased WO2002048401A2 (en) 2000-12-11 2001-12-10 Nested oligonucleotides containing hairpin for nucleic acid amplification

Country Status (5)

Country Link
US (3) US6919189B2 (en)
EP (1) EP1366191A2 (en)
AU (1) AU2002230734A1 (en)
CA (1) CA2436693A1 (en)
WO (1) WO2002048401A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002092770A2 (en) * 2001-05-14 2002-11-21 Henry Hongjun Ji Novel method for cloning variable domain sequences of immunological gene repertoire
US7306906B2 (en) * 2001-09-19 2007-12-11 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US20050208627A1 (en) * 2003-09-18 2005-09-22 Bowdish Katherine S Elicitation of antibodies to self peptides in mice by immunization with dendritic cells
US20070054378A1 (en) * 2003-09-18 2007-03-08 Alexion Pharmaceuticals, Inc. Elicitation of antibodies to self peptides in mice by immunization with dendritic cells
WO2006007567A2 (en) * 2004-07-01 2006-01-19 Gen-Probe Incorporated Methods and compositions to detect nucleic acids in a biological sample
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP2012509675A (en) 2008-11-25 2012-04-26 ジェン−プロウブ インコーポレイテッド Compositions and methods for detecting small RNAs and uses thereof
CN105026573A (en) 2012-11-28 2015-11-04 艾布维兹生物公司 Preparation of gene-specific templates for the use in single primer amplification
CN106811459B (en) * 2015-11-27 2019-09-27 清华大学 A method for improving DNA cloning and assembly efficiency by using short-chain primer repression
ES2996699T3 (en) * 2016-04-12 2025-02-13 Medimmune Llc Immune repertoire mining
US12043856B2 (en) * 2016-05-24 2024-07-23 The Translational Genomics Research Institute Molecular tagging methods and sequencing libraries
TW202540416A (en) * 2024-03-04 2025-10-16 美商賽特斯公司 Methods for constructing a library of polynucleotides having nucleic acids of interest

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
US5679512A (en) * 1992-07-31 1997-10-21 Behringwerke Ag Method for introducing defined sequences at the 3'end of polynucleotides
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
IL63907A0 (en) 1980-09-24 1981-12-31 Cetus Corp Diagnostic method and antibody probe for use therein
ATE53862T1 (en) 1982-01-22 1990-06-15 Cetus Corp METHODS FOR THE CHARACTERIZATION OF HLA AND THE CDNS TEST AGENTS USED THEREIN.
US4582788A (en) 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
EP0368684B2 (en) 1988-11-11 2004-09-29 Medical Research Council Cloning immunoglobulin variable domain sequences.
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
FR2724934B1 (en) 1994-09-26 1997-01-24 Bio Merieux CHIMERIC OLIGONUCLEOTIDE AND ITS USE IN OBTAINING NUCLEIC ACID TRANSCRIPTS
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6277607B1 (en) * 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
MXPA02002656A (en) 1999-09-13 2003-10-14 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences.
US6582938B1 (en) * 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US7306906B2 (en) * 2001-09-19 2007-12-11 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5679512A (en) * 1992-07-31 1997-10-21 Behringwerke Ag Method for introducing defined sequences at the 3'end of polynucleotides
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZEBEDEE S L ET AL: "HUMAN COMBINATORIAL ANTIBODY LIBRARIES TO HEPATITIS B SURFACE ANTIGEN", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, 1 April 1992 (1992-04-01), pages 3175 - 3179, XP002031594, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
US20050079489A1 (en) 2005-04-14
USRE41365E1 (en) 2010-06-01
WO2002048401A2 (en) 2002-06-20
AU2002230734A1 (en) 2002-06-24
CA2436693A1 (en) 2002-06-20
US20040101886A1 (en) 2004-05-27
US6919189B2 (en) 2005-07-19
EP1366191A2 (en) 2003-12-03

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