WO2002048673A3 - Method for orthogonal analyte stacking/injection systems in electrophoresis - Google Patents

Method for orthogonal analyte stacking/injection systems in electrophoresis Download PDF

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Publication number
WO2002048673A3
WO2002048673A3 PCT/US2001/043259 US0143259W WO0248673A3 WO 2002048673 A3 WO2002048673 A3 WO 2002048673A3 US 0143259 W US0143259 W US 0143259W WO 0248673 A3 WO0248673 A3 WO 0248673A3
Authority
WO
WIPO (PCT)
Prior art keywords
injection
electrokinetic
analytes
stacking
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/043259
Other languages
French (fr)
Other versions
WO2002048673A2 (en
Inventor
James P Landers
James F Palmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UVA Licensing and Ventures Group
University of Virginia UVA
Original Assignee
University of Virginia UVA
University of Virginia Patent Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Virginia UVA, University of Virginia Patent Foundation filed Critical University of Virginia UVA
Priority to AU2002241480A priority Critical patent/AU2002241480A1/en
Priority to EP01988144A priority patent/EP1355858A2/en
Priority to US10/432,141 priority patent/US7223325B2/en
Publication of WO2002048673A2 publication Critical patent/WO2002048673A2/en
Publication of WO2002048673A3 publication Critical patent/WO2002048673A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N2030/162—Injection electromigration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

In the present capillary electrokinetic chromatograpy method, analytes are injected by electroosmotic flow directly from a sample matrix into a separation buffer containing an electrokinetic vector with an opposite mobility. Analytes can now be injected at the velocity of electroosmotic flow, but are retained at the interface of the sample matrix-co-ion and separation buffer micelle zones as analyte/micelle complexes. Manipulation of the injecting force and opposing stacking force allow greatly increased length or volume of injection. Concentrations of the micelle, methanol, and borate in the separation buffer were provided to increase maximum injection length of neutral analytes. Reducing the analyte velocity in the separation buffer without substantially decreasing the velocity of the analyte during injection from the sample vial allowed greatly extended sample plug injection lengths. It is further enabled to inject sample solvent volumes equivalent to about twenty times the effective capillary volume. Equations and algorithms describing the injection process and maximum injection lengths for this mode of stacking in electrokinetic capillary chromatography are introduced. Use of the present method provides for maximum electrokinetic stacking injection for a wide variety of analytes and separation systems.
PCT/US2001/043259 1999-10-15 2001-11-19 Method for orthogonal analyte stacking/injection systems in electrophoresis Ceased WO2002048673A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002241480A AU2002241480A1 (en) 2000-11-17 2001-11-19 Method for orthogonal analyte stacking/injection systems in electrophoresis
EP01988144A EP1355858A2 (en) 2000-11-17 2001-11-19 Method for orthogonal analyte stacking/injection systems in electrophoresis
US10/432,141 US7223325B2 (en) 1999-10-15 2001-11-19 Method for orthogonal analyte stacking/injection systems in electrophoresis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24961100P 2000-11-17 2000-11-17
US60/249,611 2000-11-17

Publications (2)

Publication Number Publication Date
WO2002048673A2 WO2002048673A2 (en) 2002-06-20
WO2002048673A3 true WO2002048673A3 (en) 2002-08-15

Family

ID=22944247

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/043259 Ceased WO2002048673A2 (en) 1999-10-15 2001-11-19 Method for orthogonal analyte stacking/injection systems in electrophoresis

Country Status (3)

Country Link
EP (1) EP1355858A2 (en)
AU (1) AU2002241480A1 (en)
WO (1) WO2002048673A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7718046B2 (en) * 2004-06-10 2010-05-18 The United States Of America, As Represented By The Secretary Of Commerce, The National Institute Of Standards & Technology Micellar gradient focusing
US11207677B2 (en) 2018-03-07 2021-12-28 University Of Virginia Patent Foundation Devices, systems, and methods for detecting substances
CN108562637A (en) * 2018-04-18 2018-09-21 福州大学 A kind of electronic extracting method of polar substances based on Capillary Electrophoresis

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340452A (en) * 1991-02-01 1994-08-23 Beckman Instruments, Inc. On-column preconcentration of samples in capillary electrophoresis
US5800691A (en) * 1996-12-23 1998-09-01 Guest Elchrom Scientific Ag Electrophoresis gels containing sample wells with enlarged loading area
US5843294A (en) * 1988-11-29 1998-12-01 Isco, Inc. Capillary electrophoresis sample injection technique
US6010607A (en) * 1994-08-01 2000-01-04 Lockheed Martin Energy Research Corporation Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis
US6045676A (en) * 1996-08-26 2000-04-04 The Board Of Regents Of The University Of California Electrochemical detector integrated on microfabricated capilliary electrophoresis chips
US6214191B1 (en) * 1998-05-22 2001-04-10 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6319379B1 (en) * 1999-08-23 2001-11-20 The Regents Of The University Of California Modified electrokinetic sample injection method in chromatography and electrophoresis analysis

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843294A (en) * 1988-11-29 1998-12-01 Isco, Inc. Capillary electrophoresis sample injection technique
US5340452A (en) * 1991-02-01 1994-08-23 Beckman Instruments, Inc. On-column preconcentration of samples in capillary electrophoresis
US6010607A (en) * 1994-08-01 2000-01-04 Lockheed Martin Energy Research Corporation Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis
US6045676A (en) * 1996-08-26 2000-04-04 The Board Of Regents Of The University Of California Electrochemical detector integrated on microfabricated capilliary electrophoresis chips
US5800691A (en) * 1996-12-23 1998-09-01 Guest Elchrom Scientific Ag Electrophoresis gels containing sample wells with enlarged loading area
US6214191B1 (en) * 1998-05-22 2001-04-10 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6319379B1 (en) * 1999-08-23 2001-11-20 The Regents Of The University Of California Modified electrokinetic sample injection method in chromatography and electrophoresis analysis

Also Published As

Publication number Publication date
AU2002241480A1 (en) 2002-06-24
WO2002048673A2 (en) 2002-06-20
EP1355858A2 (en) 2003-10-29

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