WO2002072876A3 - Screening method for the selection of peptides or proteins comprising discrete dimension assays, gene mutation and application assays - Google Patents

Screening method for the selection of peptides or proteins comprising discrete dimension assays, gene mutation and application assays Download PDF

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Publication number
WO2002072876A3
WO2002072876A3 PCT/DK2002/000160 DK0200160W WO02072876A3 WO 2002072876 A3 WO2002072876 A3 WO 2002072876A3 DK 0200160 W DK0200160 W DK 0200160W WO 02072876 A3 WO02072876 A3 WO 02072876A3
Authority
WO
WIPO (PCT)
Prior art keywords
assays
actives
peptides
proteins
nucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2002/000160
Other languages
French (fr)
Other versions
WO2002072876A2 (en
Inventor
Soeren Kjaerulf
Michael Lamsa
Thomas Thisted
Peter Kamp Hansen
Henrik Pedersen
Henrik Frisner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to US10/471,269 priority Critical patent/US20040132039A1/en
Priority to CA002440841A priority patent/CA2440841A1/en
Priority to AU2002237216A priority patent/AU2002237216A1/en
Priority to EP02703528A priority patent/EP1370652A2/en
Publication of WO2002072876A2 publication Critical patent/WO2002072876A2/en
Publication of WO2002072876A3 publication Critical patent/WO2002072876A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Ecology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a screening method for identifying actives (peptides or proteins) with improved performance comprising: (1) screening a library of actives encoded by nucleotide sequences in at least two different discrete dimension assays employing selected parameters and selecting the actives exhibiting improved properties in the discrete dimension assays, (2) collecting nucleotide sequences encoding the selected actives, (3) mutating collected nucleotide sequences, (4) screening actives encoded by the mutated nucleotide sequences in an application assay and selecting actives, which exhibit improved performance.
PCT/DK2002/000160 2001-03-12 2002-03-12 Screening method for the selection of peptides or proteins comprising discrete dimension assays, gene mutation and application assays Ceased WO2002072876A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/471,269 US20040132039A1 (en) 2001-03-12 2002-03-12 Screening method comprising combination of discrete dimension assay testing of actives, gene mutation and application assay testing of selected actives
CA002440841A CA2440841A1 (en) 2001-03-12 2002-03-12 Screening method for the selection of peptides or proteins comprising discrete dimension assays, gene mutation and application assays
AU2002237216A AU2002237216A1 (en) 2001-03-12 2002-03-12 Screening method for the selection of peptides or proteins comprising discrete dimension assays, gene mutation and application assays
EP02703528A EP1370652A2 (en) 2001-03-12 2002-03-12 Screening method comprising combination of discrete dimension assay testing of actives, gene mutation and application assay testing of selected actives

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27516401P 2001-03-12 2001-03-12
US60/275,164 2001-03-12

Publications (2)

Publication Number Publication Date
WO2002072876A2 WO2002072876A2 (en) 2002-09-19
WO2002072876A3 true WO2002072876A3 (en) 2002-12-12

Family

ID=23051133

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000160 Ceased WO2002072876A2 (en) 2001-03-12 2002-03-12 Screening method for the selection of peptides or proteins comprising discrete dimension assays, gene mutation and application assays

Country Status (5)

Country Link
US (1) US20040132039A1 (en)
EP (1) EP1370652A2 (en)
AU (1) AU2002237216A1 (en)
CA (1) CA2440841A1 (en)
WO (1) WO2002072876A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7018805B2 (en) * 2002-08-29 2006-03-28 Vicam L.P. α-Amylase assay and uses thereof
US8383346B2 (en) * 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998041622A1 (en) * 1997-03-18 1998-09-24 Novo Nordisk A/S Method for constructing a library using dna shuffling
WO2000009682A1 (en) * 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100258460B1 (en) * 1991-05-01 2000-06-01 한센 핀 베네드 Stabilized enzymes and detergent compositions
US5939268A (en) * 1994-07-26 1999-08-17 The Scripps Research Institute Combinatorial libraries of molecules and methods for producing same
US6309871B1 (en) * 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998041622A1 (en) * 1997-03-18 1998-09-24 Novo Nordisk A/S Method for constructing a library using dna shuffling
WO2000009682A1 (en) * 1998-08-12 2000-02-24 Maxygen, Inc. Dna shuffling of monooxygenase genes for production of industrial chemicals

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GIVER L ET AL: "Directed evolution of a thermostable esterase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, no. 22, 27 October 1998 (1998-10-27), pages 12809 - 12813, XP002209305, ISSN: 0027-8424 *
NESS JON E ET AL: "DNA shuffling of subgenomic sequences of subtilisin", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 17, no. 9, September 1999 (1999-09-01), pages 893 - 896, XP002191112, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
EP1370652A2 (en) 2003-12-17
CA2440841A1 (en) 2002-09-19
WO2002072876A2 (en) 2002-09-19
US20040132039A1 (en) 2004-07-08
AU2002237216A1 (en) 2002-09-24

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