WO2002082078A3 - Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes - Google Patents

Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes Download PDF

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Publication number
WO2002082078A3
WO2002082078A3 PCT/EP2002/003890 EP0203890W WO02082078A3 WO 2002082078 A3 WO2002082078 A3 WO 2002082078A3 EP 0203890 W EP0203890 W EP 0203890W WO 02082078 A3 WO02082078 A3 WO 02082078A3
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WO
WIPO (PCT)
Prior art keywords
analytes
utilized
molecules
affinity
quantifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/003890
Other languages
French (fr)
Other versions
WO2002082078A2 (en
Inventor
Reinhard Bredehorst
Rainer Hintsche
Anton Heuberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Original Assignee
Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV filed Critical Fraunhofer Gesellschaft zur Foerderung der Angewandten Forschung eV
Priority to EP02724275A priority Critical patent/EP1448991A2/en
Publication of WO2002082078A2 publication Critical patent/WO2002082078A2/en
Anticipated expiration legal-status Critical
Publication of WO2002082078A3 publication Critical patent/WO2002082078A3/en
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/586—Liposomes, microcapsules or cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Analytes bound to immobilized capture molecules by molecular biological or immunochemical reactions are detected or quantified with affinity liposomes containing encapsulated enzyme activators and surface-attached affinity components capable of specific binding to captured analytes or derivatives thereof in a structure restricted manner. Enzyme activators encapsulated in analyte-bound affinity liposomes are released by temperature- or detergent-mediated mechanisms and utilized for activation of solid phase-immobilized inactive enzyme molecules which in turn are utilized to generate colorimetric, fluorescent, chemiluminescent, or bioluminescent reporter molecules the quantity of which is a proportiona measure of the amount of analyte in the specimen. For amplified assay procedures polymeric carrier molecules capable of binding multiple affinity liposomes and/or preformed complexes of affinity liposomes are utilized.
PCT/EP2002/003890 2001-04-09 2002-04-08 Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes Ceased WO2002082078A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02724275A EP1448991A2 (en) 2001-04-09 2002-04-08 Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens, antibodies and other analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28208801P 2001-04-09 2001-04-09
US60/282,088 2001-04-09

Publications (2)

Publication Number Publication Date
WO2002082078A2 WO2002082078A2 (en) 2002-10-17
WO2002082078A3 true WO2002082078A3 (en) 2004-06-10

Family

ID=23080057

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/003890 Ceased WO2002082078A2 (en) 2001-04-09 2002-04-08 Activated enzyme-linked detection systems for detecting and quantifying nucleid acids, antigens antibodies and other analytes

Country Status (2)

Country Link
EP (1) EP1448991A2 (en)
WO (1) WO2002082078A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2748235C2 (en) * 2015-12-29 2021-05-21 Гальдерма С.А. Method for deacetylation of biopolymers

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JP4457107B2 (en) * 2003-03-25 2010-04-28 ジーイー・ヘルスケア・バイオ−サイエンシーズ・アーベー Immobilization method and kit therefor
US8741577B2 (en) * 2003-04-07 2014-06-03 Bio-Rad Laboratories Inc. Surface immobilised multilayer structure of vesicles
CA2521613C (en) * 2003-04-07 2015-03-31 Layerlab Aktiebolag Surface-immobilised multilayer structure of vesicles
CA2564426C (en) * 2003-09-02 2013-01-08 Expressive Constructs, Inc. A device for the detection of microorganisms using signal amplification
FR2918755B1 (en) * 2007-07-09 2009-10-30 Commissariat Energie Atomique METHOD FOR DETECTING AND QUANTIFYING SOLID - SUPPORTED ANALYTES WITH FLUORESCENT MOLECULES ENCAPSULATED IN IMMUNOLIPOSOMES.
EP2225547B1 (en) * 2007-12-20 2016-07-27 Koninklijke Philips N.V. Microelectronic sensor device for the detection of target particles
DE102008027038A1 (en) 2008-06-06 2009-12-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Method for detecting chemical or biological species and electrode arrangement therefor
NZ590411A (en) * 2008-07-11 2013-07-26 Universal Biosensors Pty Ltd Enhanced immunoassay sensor
US8383337B2 (en) * 2008-07-18 2013-02-26 General Electric Company Methods using metal oxide particles for analyte detection
CN103645185A (en) * 2013-12-17 2014-03-19 武汉大学 Method for visually detecting alkaline phosphatase
EP3252082A1 (en) * 2016-05-31 2017-12-06 Galderma S.A. Method for deacetylation of biopolymers
PT3623390T (en) * 2016-05-31 2023-10-27 Galderma Sa Carbohydrate crosslinker
AU2020395955A1 (en) 2019-12-02 2022-07-14 Galderma Holding SA High molecular weight esthetic compositions
CN111579613B (en) * 2020-05-12 2022-07-08 台州学院 Photoelectric chemical sensing-based fatty acid binding protein detection method
WO2022040484A1 (en) * 2020-08-19 2022-02-24 The Regents Of The University Of California Diagnostic methods and compositions
CN116948169B (en) * 2023-06-14 2024-06-28 华南师范大学 A rapid synthesis method and application of self-enhanced electrochemiluminescence probe based on polylysine in water phase

Citations (4)

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Publication number Priority date Publication date Assignee Title
US4939098A (en) * 1985-05-31 1990-07-03 Hitachi, Ltd. Immunoassay and measurement kit used therefor
WO1999010743A1 (en) * 1997-08-29 1999-03-04 Regents Of The University Of California Sol-gel matrices for direct colorimetric detection of analytes
EP0926497A2 (en) * 1997-12-26 1999-06-30 Hogy Medical Co., Ltd. Colorimetric sensor employing polydiacetylene membrane
WO1999060399A1 (en) * 1998-05-21 1999-11-25 Cornell Research Foundation, Inc. Liposome-enhanced test device and method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4939098A (en) * 1985-05-31 1990-07-03 Hitachi, Ltd. Immunoassay and measurement kit used therefor
WO1999010743A1 (en) * 1997-08-29 1999-03-04 Regents Of The University Of California Sol-gel matrices for direct colorimetric detection of analytes
EP0926497A2 (en) * 1997-12-26 1999-06-30 Hogy Medical Co., Ltd. Colorimetric sensor employing polydiacetylene membrane
WO1999060399A1 (en) * 1998-05-21 1999-11-25 Cornell Research Foundation, Inc. Liposome-enhanced test device and method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BAEUMNER A J ET AL: "Development of a new immunosensor for pesticide detection: a disposable system with liposome-enhancement and amperometric detection", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 13, 1998, pages 519 - 529, XP002217793, ISSN: 0956-5663 *
WARSINKE A ET AL: "Electrochemical immunoassays", FRESENIUS J ANAL CHEM, SPRINGER-VERLAG, HEIDELBERG, DE, vol. 366, no. 6-7, March 2000 (2000-03-01), pages 622 - 634, XP002267091 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2748235C2 (en) * 2015-12-29 2021-05-21 Гальдерма С.А. Method for deacetylation of biopolymers

Also Published As

Publication number Publication date
EP1448991A2 (en) 2004-08-25
WO2002082078A2 (en) 2002-10-17

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