WO2002103050A3 - Virus detection method, primers therefor and screening kit - Google Patents

Virus detection method, primers therefor and screening kit Download PDF

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Publication number
WO2002103050A3
WO2002103050A3 PCT/GB2002/002847 GB0202847W WO02103050A3 WO 2002103050 A3 WO2002103050 A3 WO 2002103050A3 GB 0202847 W GB0202847 W GB 0202847W WO 02103050 A3 WO02103050 A3 WO 02103050A3
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WO
WIPO (PCT)
Prior art keywords
virus
extension product
primer extension
nucleic acid
self
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2002/002847
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French (fr)
Other versions
WO2002103050A2 (en
Inventor
Keith William Hart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cardiff University
Original Assignee
University of Wales College of Medicine UWCM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0114430A external-priority patent/GB0114430D0/en
Priority claimed from GB0207276A external-priority patent/GB0207276D0/en
Application filed by University of Wales College of Medicine UWCM filed Critical University of Wales College of Medicine UWCM
Priority to JP2003505371A priority Critical patent/JP2004533256A/en
Priority to US10/480,852 priority patent/US20060057561A1/en
Priority to EP02738381A priority patent/EP1409730A2/en
Priority to CA002450164A priority patent/CA2450164A1/en
Publication of WO2002103050A2 publication Critical patent/WO2002103050A2/en
Anticipated expiration legal-status Critical
Publication of WO2002103050A3 publication Critical patent/WO2002103050A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Discloses a novel detection and typing method for viruses, such as human papillomaviruses, based on real-time PCR using self-probing amplicon fluorescent primers. The method comprises: (IA) contacting the sample with a self-probing amplicon ('virus self-probing amplicon') comprising (i) a virus primer capable of hybridising to at least one target viral nucleic acid sequence and undergoing amplification thereof under primer amplification conditions to form a virus primer extension product; (ii) a virus probe comprising a nucleic acid sequence complementary to a target sequence of the virus primer extension product and capable of hybridisation thereto, provided that the self-probing amplicon is adapted to ensure that the virus probe is unresponsive to amplification under the primer amplification conditions; and (iii) a member of a virus signalling system, which system is capable of causing a detectable signal to be effected on hybridisation of the virus probe sequence to the virus primer extension product, whereby presence or absence of the target viral nucleic acid sequence in the sample is indicated by the detectable signal; (IB) amplifying the product of step (IA) under the primer amplification conditions to an extent enabling the detectable signal to be effected after step (II); and (II) separating the virus primer extension product from the target viral nucleic acid sequence; allowing the virus probe to hybridise to the target sequence of the virus primer extension product; and monitoring the signalling system. This method is quick, simple, specific, sensitive, and capable of estimating viral load per cell. The results of over 100 HPV typing reactions performed on cell lines, biopsies and cervical cytobrush samples are given which, when compared to the current reference HPV detection and typing technique, present a kappa value of 0.89. The method is also applicable to other viruses, such as SV40.
PCT/GB2002/002847 2001-06-14 2002-06-13 Virus detection method, primers therefor and screening kit Ceased WO2002103050A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003505371A JP2004533256A (en) 2001-06-14 2002-06-13 Virus detection method, virus detection primer, and screening kit
US10/480,852 US20060057561A1 (en) 2001-06-14 2002-06-13 Virus detection method, primers therefor and screening kit
EP02738381A EP1409730A2 (en) 2001-06-14 2002-06-13 Virus detection method, primers therefor and screening kit
CA002450164A CA2450164A1 (en) 2001-06-14 2002-06-13 Virus detection method, primers therefor and screening kit

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0114430A GB0114430D0 (en) 2001-06-14 2001-06-14 Detection method
GB0114430.2 2001-06-14
GB0207276A GB0207276D0 (en) 2002-03-28 2002-03-28 Detection method
GB0207276.7 2002-03-28

Publications (2)

Publication Number Publication Date
WO2002103050A2 WO2002103050A2 (en) 2002-12-27
WO2002103050A3 true WO2002103050A3 (en) 2004-02-26

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Family Applications (1)

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PCT/GB2002/002847 Ceased WO2002103050A2 (en) 2001-06-14 2002-06-13 Virus detection method, primers therefor and screening kit

Country Status (5)

Country Link
US (1) US20060057561A1 (en)
EP (1) EP1409730A2 (en)
JP (1) JP2004533256A (en)
CA (1) CA2450164A1 (en)
WO (1) WO2002103050A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111733298A (en) * 2020-08-25 2020-10-02 圣湘生物科技股份有限公司 Composition, kit and method for detecting and typing polyoma virus and application thereof

Families Citing this family (16)

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US7357188B1 (en) 1998-12-07 2008-04-15 Shell Oil Company Mono-diameter wellbore casing
US7838225B2 (en) * 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
DE10338308B4 (en) * 2003-08-15 2006-10-19 Epigenomics Ag Method for the detection of cytosine methylations in DNA
WO2005064020A1 (en) 2003-12-23 2005-07-14 Autogenomics, Inc. Multiplexed nucleic acid analysis with high specificity
WO2005100610A2 (en) * 2004-04-15 2005-10-27 University College Cardiff Consultants Limited Virus detection method, primers therefor and screening kit
DE602005022759D1 (en) 2004-12-08 2010-09-16 Gen Probe Inc NUCLEIC ACIDIC ACID DETECTION FROM VARIOUS TYPES OF THE HUMAN PAPILLOMA VIRUS
WO2007115582A1 (en) 2006-04-11 2007-10-18 Bio-Rad Pasteur Hpv detection and quantification by real-time multiplex amplification
DE102006041970A1 (en) * 2006-08-28 2008-03-06 Genome Identification Diagnostics Gmbh Method and means for detecting human papillomavirus
GB0702557D0 (en) 2007-02-09 2007-03-21 Health Prot Agency Detection of human papillomavirus
EP1997914A1 (en) * 2007-06-01 2008-12-03 Università Degli Studi Di Milano - Bicocca Identification and quantification of oncogenic HPV nucleic acids by means of real-time PCR assays
WO2011084619A2 (en) * 2009-12-16 2011-07-14 Mark Stahl Elimination of pathogenic infection in farmed animal populations
BR112012018394B8 (en) * 2009-12-21 2021-07-27 Seegene Inc method for detecting a target nucleic acid sequence and kit for detecting a target nucleic acid sequence
BR112014000138B1 (en) 2011-07-06 2021-11-16 Quest Diagnostics Investments Incorporation METHOD FOR IDENTIFYING THE PRESENCE OR ABSENCE OF A TARGET NUCLEIC ACID
EP3052656B1 (en) 2013-09-30 2018-12-12 President and Fellows of Harvard College Methods of determining polymorphisms
WO2017117287A1 (en) * 2015-12-30 2017-07-06 Bio-Rad Laboratories, Inc. Split-cycle and tape amplification
CN110904278B (en) * 2019-12-30 2023-01-17 申友基因组研究院(南京)有限公司 Type 13 high-risk HPV multiplex fluorescence detection kit

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993002215A1 (en) * 1991-07-24 1993-02-04 Royal Free Hospital School Of Medicine Quantitative nucleic acid amplification
WO1993023566A1 (en) * 1992-05-21 1993-11-25 Isis Innovation Limited Nucleic acid detection and quantification
WO1995022626A1 (en) * 1994-02-21 1995-08-24 Stichting Researchfonds Pathologie Human papilloma virus detection in a nucleic acid amplification process using general primers
US5639871A (en) * 1988-09-09 1997-06-17 Roche Molecular Systems, Inc. Detection of human papillomavirus by the polymerase chain reaction

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639871A (en) * 1988-09-09 1997-06-17 Roche Molecular Systems, Inc. Detection of human papillomavirus by the polymerase chain reaction
WO1993002215A1 (en) * 1991-07-24 1993-02-04 Royal Free Hospital School Of Medicine Quantitative nucleic acid amplification
WO1993023566A1 (en) * 1992-05-21 1993-11-25 Isis Innovation Limited Nucleic acid detection and quantification
WO1995022626A1 (en) * 1994-02-21 1995-08-24 Stichting Researchfonds Pathologie Human papilloma virus detection in a nucleic acid amplification process using general primers

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
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JORDENS J ZOE ET AL: "Amplification with molecular beacon primers and reverse line blotting for the detection and typing of human papillomaviruses.", JOURNAL OF VIROLOGICAL METHODS, vol. 89, no. 1-2, September 2000 (2000-09-01), pages 29 - 37, XP002250695, ISSN: 0166-0934 *
PARK JONG SUP ET AL: "Physical status and expression of HPV genes in cervical cancers.", GYNECOLOGIC ONCOLOGY, vol. 65, no. 1, 1997, pages 121 - 129, XP002250696, ISSN: 0090-8258 *
THELWELL N ET AL: "Mode of action and application of Scorpion primers to mutation detection", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 28, no. 19, 1 October 2000 (2000-10-01), pages 3752 - 3761, XP002247004, ISSN: 0305-1048 *
WHITCOMBE D ET AL: "Detection of PCR products using self-probing amplicons and fluorescence", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, no. 8, August 1999 (1999-08-01), pages 804 - 807, XP002226672, ISSN: 1087-0156 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111733298A (en) * 2020-08-25 2020-10-02 圣湘生物科技股份有限公司 Composition, kit and method for detecting and typing polyoma virus and application thereof
CN111733298B (en) * 2020-08-25 2020-11-20 圣湘生物科技股份有限公司 Composition, kit and method for detecting and typing polyoma virus and application thereof

Also Published As

Publication number Publication date
CA2450164A1 (en) 2002-12-27
JP2004533256A (en) 2004-11-04
WO2002103050A2 (en) 2002-12-27
US20060057561A1 (en) 2006-03-16
EP1409730A2 (en) 2004-04-21

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