WO2003010193A3 - Genes and proteins for the biosynthesis of rosaramicin - Google Patents

Genes and proteins for the biosynthesis of rosaramicin Download PDF

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Publication number
WO2003010193A3
WO2003010193A3 PCT/CA2002/001177 CA0201177W WO03010193A3 WO 2003010193 A3 WO2003010193 A3 WO 2003010193A3 CA 0201177 W CA0201177 W CA 0201177W WO 03010193 A3 WO03010193 A3 WO 03010193A3
Authority
WO
WIPO (PCT)
Prior art keywords
rosaramicin
biosynthesis
genes
proteins
macrolides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2002/001177
Other languages
French (fr)
Other versions
WO2003010193A2 (en
Inventor
Chris M Farnet
Alfredo Staffa
Xianshu Yang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thallion Pharmaceuticals Inc
Original Assignee
Ecopia Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecopia Biosciences Inc filed Critical Ecopia Biosciences Inc
Priority to EP02750735A priority Critical patent/EP1409686B1/en
Priority to DE60210096T priority patent/DE60210096D1/en
Priority to AU2002355157A priority patent/AU2002355157A1/en
Publication of WO2003010193A2 publication Critical patent/WO2003010193A2/en
Publication of WO2003010193A3 publication Critical patent/WO2003010193A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Genes and proteins involved in the biosynthesis of macrolides by microorganisms, in particular the nucleic acids forming the biosynthetic locus for the 16-member macrolide rosaramicin from Micromonospora carbonacea. These nucleic acids can be used to make expression constructs and transformed host cells for the production of rosaramicin. The genes and proteins allow direct manipulation of macrolides and related chemical structures via chemical engineering of the proteins involved in the biosynthesis of rosaramicin.
PCT/CA2002/001177 2001-07-26 2002-07-26 Genes and proteins for the biosynthesis of rosaramicin Ceased WO2003010193A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02750735A EP1409686B1 (en) 2001-07-26 2002-07-26 Genes and proteins for the biosynthesis of rosaramicin
DE60210096T DE60210096D1 (en) 2001-07-26 2002-07-26 GENES AND PROTEINS FOR ROSARAMICIN BIOSYNTHESIS
AU2002355157A AU2002355157A1 (en) 2001-07-26 2002-07-26 Genes and proteins for the biosynthesis of rosaramicin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30762901P 2001-07-26 2001-07-26
US60/307,629 2001-07-26

Publications (2)

Publication Number Publication Date
WO2003010193A2 WO2003010193A2 (en) 2003-02-06
WO2003010193A3 true WO2003010193A3 (en) 2003-04-10

Family

ID=23190548

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2002/001177 Ceased WO2003010193A2 (en) 2001-07-26 2002-07-26 Genes and proteins for the biosynthesis of rosaramicin

Country Status (7)

Country Link
US (1) US20030113874A1 (en)
EP (1) EP1409686B1 (en)
AT (1) ATE321139T1 (en)
AU (1) AU2002355157A1 (en)
CA (1) CA2391131C (en)
DE (1) DE60210096D1 (en)
WO (1) WO2003010193A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2851773A1 (en) * 2003-02-27 2004-09-03 Aventis Pharma Sa New polynucleotides encoding proteins involved in spiramycin biosynthesis, useful for improving synthesis of macrolide antibiotics or for generating new hybrid macrolides
US7579167B2 (en) 2002-10-08 2009-08-25 Aventis Pharma S. Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof
BRPI0314543B8 (en) * 2002-10-08 2021-05-25 Aventis Pharma Sa host cells, recombinant DNA, their use, processes for the production of a polypeptide involved in the biosynthesis of spiramycins, the production of macrolides and the production of spiramycin(s), and macrolide-producing mutant transgenic microorganism
WO2012035055A1 (en) 2010-09-17 2012-03-22 Glaxo Group Limited Novel compounds
EP4667564A2 (en) * 2013-08-14 2025-12-24 Institute Of Genetics And Developmental Biology, Chinese Academy of Sciences Methods of modulating seed and organ size in plants
WO2017100650A1 (en) 2015-12-09 2017-06-15 Vanderbilt University Biosynthesis of everninomicin analogs in micromonospora carbonacea var aurantiaca

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234690A (en) * 1979-07-16 1980-11-18 Schering Corporation Method for producing rosaramicin (rosamicin)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5149639A (en) * 1986-03-24 1992-09-22 Abbott Laboratories Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production
US4874748A (en) * 1986-03-24 1989-10-17 Abbott Laboratories Cloning vectors for streptomyces and use thereof in macrolide antibiotic production
US5063155A (en) * 1988-03-28 1991-11-05 Eli Lilly And Company Method for producing 2"'-o-demethyltylosin
US5098837A (en) * 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
EP0725778B1 (en) * 1993-09-20 2001-09-26 The Leland Stanford Junior University Recombinant production of novel polyketides
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6361974B1 (en) * 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4234690A (en) * 1979-07-16 1980-11-18 Schering Corporation Method for producing rosaramicin (rosamicin)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BENTLEY, S.D. ET AL.: "A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome", MOLECULAR MICROBIOLOGY, vol. 21, 1996, pages 77 - 96 *
DATABASE EMBL [online] 20 April 1994 (1994-04-20), MERSON-DAVIES L.A. ET AL.: "Streptomyces fradiae cytochrome P-450, dTDP-glucose synthase, dTDP-glucose dehydratase, thioesterase, TylCVI (tylCVI), and TylR (tylR) genes", XP002228882, Database accession no. U08223 *
DATABASE EMBL [online] 8 January 1997 (1997-01-08), GANDECHA A.R.: "Streptomyces fradiae tylMIII(orf1*), tylMII(orf2*), tylMI(orf3*) and ccr(orf4*) genes", XP002228883, Database accession no. X81885 *
DATABASE SWALL [online] 1 May 1999 (1999-05-01), SEEGER, K.J. ET AL.: "Crotonyl CoA reductase", XP002228884, Database accession no. Q9ZBK1 *
MERSON-DAVIES L.A. ET AL.: "Analysis of five tylosin biosynthetic genes from the tylIBA region of the Streptomyces fradiae genome", MOLECULAR MICROBIOLOGY, vol. 13, 1994, pages 349 - 355, XP009046662, DOI: doi:10.1111/j.1365-2958.1994.tb00428.x *

Also Published As

Publication number Publication date
EP1409686A2 (en) 2004-04-21
US20030113874A1 (en) 2003-06-19
EP1409686B1 (en) 2006-03-22
ATE321139T1 (en) 2006-04-15
CA2391131A1 (en) 2002-11-19
DE60210096D1 (en) 2006-05-11
CA2391131C (en) 2004-10-12
AU2002355157A1 (en) 2003-02-17
WO2003010193A2 (en) 2003-02-06

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