WO2003020748A1 - Anti-cancer cyclopenta[g]quinazoline compounds - Google Patents

Anti-cancer cyclopenta[g]quinazoline compounds Download PDF

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WO2003020748A1
WO2003020748A1 PCT/GB2002/003979 GB0203979W WO03020748A1 WO 2003020748 A1 WO2003020748 A1 WO 2003020748A1 GB 0203979 W GB0203979 W GB 0203979W WO 03020748 A1 WO03020748 A1 WO 03020748A1
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group
tetrazol
cyclopenta
alkyl
quinazoline
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French (fr)
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Vassilios Bavetsias
Ann Lesley Jackman
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BTG International Ltd
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BTG International Ltd
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Priority claimed from GB0121151A external-priority patent/GB0121151D0/en
Priority claimed from GB0129387A external-priority patent/GB0129387D0/en
Priority to DK02755281.9T priority Critical patent/DK1421105T3/en
Priority to KR1020047002994A priority patent/KR100983962B1/en
Priority to AU2002321576A priority patent/AU2002321576B2/en
Priority to EP02755281A priority patent/EP1421105B1/en
Priority to DE60237902T priority patent/DE60237902D1/en
Priority to CA2458813A priority patent/CA2458813C/en
Priority to BRPI0212215A priority patent/BRPI0212215B8/en
Priority to AT02755281T priority patent/ATE483722T1/en
Application filed by BTG International Ltd filed Critical BTG International Ltd
Priority to US10/487,871 priority patent/US7297701B2/en
Priority to MXPA04001905A priority patent/MXPA04001905A/en
Priority to JP2003525018A priority patent/JP4328202B2/en
Publication of WO2003020748A1 publication Critical patent/WO2003020748A1/en
Priority to ZA2004/01587A priority patent/ZA200401587B/en
Anticipated expiration legal-status Critical
Priority to US11/710,985 priority patent/US7863284B2/en
Priority to US11/727,242 priority patent/US7705006B2/en
Priority to US12/588,910 priority patent/US8486955B2/en
Priority to US12/659,286 priority patent/US8063056B2/en
Priority to US12/801,853 priority patent/US8552016B2/en
Ceased legal-status Critical Current

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    • C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
    • C07D239/72—Quinazolines; Hydrogenated quinazolines
    • C07D239/86—Quinazolines; Hydrogenated quinazolines with hetero atoms directly attached in position 4
    • C07D239/88—Oxygen atoms
    • C07D239/90—Oxygen atoms with acyclic radicals attached in position 2 or 3
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    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
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    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
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    • A61K9/2013—Organic compounds, e.g. phospholipids, fats
    • A61K9/2018—Sugars, or sugar alcohols, e.g. lactose, mannitol; Derivatives thereof, e.g. polysorbates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
    • C07K5/0215—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link containing natural amino acids, forming a peptide bond via their side chain functional group, e.g. epsilon-Lys, gamma-Glu

Definitions

  • This invention relates to novel anti-cancer agents and more particularly it relates to cyclopenta[g]quinazoline derivatives which possess antiproliferative activity.
  • One group of anti-cancer agents comprises antimetabolites having antifolate activity, such as the dihydrofolate reductase inhibitor, methotrexate and the thymidylate synthase (TS) inhibitors CB3717, raltitrexed and ZD9331.
  • CB3717 is described and claimed in EP-B-0031237, raltitrexed in EP-B-0239362 and ZD9331 in EP-B-0562734.
  • TS inhibitors have demonstrable clinical activity in a range of solid tumours (see Cancer Treatment Reports, 1986, 70, 1335 and Beale et al., "Tomudex: Clinical Development” in Antifolate Drugs in Cancer Therapy (ed. Jackman), Humana Press, Totowa, New Jersey, USA, pp. 177-181, 1999).
  • Side- effects of raltitrexed and ZD9331 are predominantly related to inhibition of TS in gut and bone-marrow.
  • TS catalyses the methylation of deoxyuridine monophosphate to produce thymidine monophosphate which is required for DNA synthesis.
  • the anticancer activity of these agents may be assessed in vitro by determining their inhibitory effect on that enzyme, and in cell cultures by their inhibitory effect on a range of mouse and human cancer cell lines (see Boyle et al, "ZD9331: Preclinical and clinical studies" in Antifolate Drugs in Cancer Therapy (ed. Jackman), Humana Press, Totowa, New Jersey, USA, pp. 243-260, 1999 and Hughes et al, "Raltitrexed (Tomudex), a highly polyglutamatable antifolate thymidylate synthase inhibitor: design and preclinical activity" in Antifolate Drugs in Cancer Therapy (ed. Jackman), Humana Press, Totowa, New Jersey, USA, pp. 147-165, 1999). More recently, cyclopenta[g]quinazoline derivatives showing a good level of activity both as regards their ability to inhibit TS and also as regards their anticancer activity against various cell lines have been developed.
  • WO-A-94/11354 (British Technology Group Limited) discloses tricyclic compound of formula: wherein R 1 is hydrogen, amino, d- 4 alkyl, d- alkoxy, C 1 - 4 hydroxyalkyl or - fluoroalkyl;
  • R 2 is hydrogen, C ⁇ _4 alkyl, C 3 -4 alkenyl, C 3 -4 alkynyl, C 2 -- 4 hydroxyalkyl C 2 - 4 halogenoalkyl or C 1 - cyanoalkyl;
  • Ar is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, d- alkyl and C ⁇ - A alkoxy; and
  • R is a group of one of the following formulae:
  • R 3 is a N-linked naturally-occurring amino acid selected from the group consisting of L-alanine, L-leucine, L-isoleucine, L-valine and L-phenylalanine.
  • L-alanine L-leucine
  • L-isoleucine L-valine
  • L-phenylalanine L-phenylalanine.
  • the L-Glu- ⁇ -D-Glu compound CB300638 also mentioned in Clinical Cancer Research, 5, November 1999 (Supplement) at #566 (Theti et al.) and Proceedings of the American Association for Cancer Research, 41, March 2000 at #33 (Jackman et al), as well as in J. Med. Chem., 2000, 43, 1910-1926, where it is disclosed on page 1923 as compound 7b.
  • WO-A-95/30673 (British Technology Group Limited) discloses cyclopenta-
  • R 1 is hydrogen, amino, C 1 - 4 alkyl, - alkoxy, C 1 - 4 hydroxyalkyl or C ⁇ _4 fluoroalkyl
  • R 2 is hydrogen, C 1 -4 alkyl, C 3 - alkenyl, C ⁇ alkynyl, C 2 - 4 hydroxyalkyl, C 2 _4 halogenoalkyl or C 1 - 4 cyanoalkyl
  • Ar 1 is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, C 1 - 4 alkyl and d-4 alkoxy; and
  • R 3 is a group of one of the following formulae:
  • the ⁇ -isoform of the folate receptor ( ⁇ -FR; membrane-associated fo late- binding protein) is a glycosylphosphatidylinositol anchored cell membrane protein that has very high affinity for folic acid and the more biologically relevant reduced- -folates (Kd ⁇ 0.1 nM).
  • the mechanism of folate internalisation is receptor-mediated endocytosis.
  • the ⁇ -FR is overexpressed in many carcinomas, particularly those of ovarian origin where it is overexpressed highly and homogeneously in 90% of cases; see Cancer Res. 51, 5329-5338, 1991 (Campbell et al, 1991).
  • high ⁇ -FR expression has been linked to aggressive, platinum resistant disease and poor prognosis— see Int. J.
  • Folate receptors ( ⁇ and ⁇ ) are expressed in some adult normal tissues (low to moderate expression). Significant expression of the ⁇ -FR is largely restricted to kidney proximal tubules and choroid plexus although it is suggested that it is localised to the apical membrane surface in these organs and therefore may not play a significant role in folate uptake from blood (Reddy and Low, ibid.). There may be a specialised function of the ⁇ -FR in the proximal tubules of the kidney to salvage folates that escape in the filtrate. The ⁇ -FR is hypothesised to be involved in cell signalling pathways.
  • ⁇ -FR is associated in membranes with the G protein G ⁇ ⁇ - 3 , and the non-receptor kinase lyn.
  • High FR expression in some tumours relative to normal tissues is being exploited in several areas of cancer medicine, including the selective tumour delivery of conjugates of folic acid and toxins, liposomes, imaging or cytotoxic agents (Reddy and Low, ibid.).
  • folic acid-deferroxamine- u l In conjugates are detected only in FR-expressing tumours and not normal tissues of mice, with the exception of kidney epithelial cells.
  • the present invention comprises a cyclopenta[g] quinazoline of formula (I) :
  • A is a group OR 0 or NR ⁇ 1 wherein R° and R 1 are each independently hydrogen, d- alkyl, C 3 -4 alkenyl, C 3 _4 alkynyl, C 2 - 4 hydroxyalkyl, C 2 - halogenoalkyl or d- cyanoalkyl, or R° and R 1 together with the intermediate N form a five- or six-membered heterocyclic ring; p is an integer in the range 1 to 4;
  • R 2 is hydrogen, C 1 - 4 alkyl, C 3 - 4 alkenyl, C 3 - 4 alkynyl, C 2 - hydroxyalkyl, C 2 - 4 halogenoalkyl or C 1 - 4 cyanoalkyl;
  • Ar 1 is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, C 1 - 4 alkyl and C 1 - 4 alkoxy; and R 3 is a group of the formula: -A'-Ar ⁇ -Y 1
  • a 1 is a bond between the ⁇ -carbon atom of the group -CO-NH-CH(CO 2 H)- and Ar 2 or is a C ⁇ _ 2 alkylene group;
  • Ar is phenylene, tetrazolediyl, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which in the case of phenylene may optionally bear one or two substituents on the ring selected from halogeno, nitro, d- 4 alkyl and d- alkoxy;
  • Y 1 is a group of the formula:
  • R is hydrogen, C 1 - 4 alkyl, C 3 _ 4 alkenyl or C 3 - 4 alkynyl;
  • Y 3 is the residue of a naturally occurring amino acid ⁇ H 2 CH(CO 2 H)Y 3 ;
  • Y 3 is a group of the formula:
  • a 4 is a C 2 _ 6 alkylene group
  • R is a group of the formula:
  • a 5 is a C ⁇ _ 6 alkylene group and R is as defined above;
  • Y 4 is carboxy, tetrazol-5-yl, N-(C ⁇ -4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d- 4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulf ⁇ nyl or tetrazol-5-ylsulfonyl; and Y 5 is the residue of a naturally occurring amino acid ⁇ H 2 CH(CO 2 H)Y 5 ; or
  • Y 5 is a group of the formula:
  • Y 5 is a group of the formula:
  • Ar 3 is phenylene, tetrazolediyl, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which in the case of phenylene may optionally bear one or two substituents on the ring selected from halogeno, nitro, d-4 alkyl and d- 4 alkoxy;
  • a 7 is a C ⁇ _ 3 alkylene or C 2 _ 3 alkenylene group;
  • Y 6 is carboxy, tetrazol-5-yl, N-(C ⁇ _ 4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C1-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulf ⁇ nyl or tetrazol-5-ylsulfonyl; or R 3 is a group of the formula:
  • A is a C 1 - 4 alkylene group
  • X is sull ⁇ nyl, sulfonyl or methylene
  • Ar 4 is l,2,3-triazol-4-yl, l,2,4-triazol-3-yl or, except when X is methylene, tetrazol-5-yl; the compound (I) optionally being in the form of a pharmaceutically acceptable salt or ester.
  • alkyl, alkenyl, alkynyl and alkylene include both straight and branched chain groups but references to individual alkyl or alkylene groups, such as "propyl", are specific for the straight chain group only. An analogous convention applies to other generic terms.
  • the numbering system used for the cyclopenta[g]quinazoline nucleus is the conventional one as shown below:
  • ⁇ -glutamyl denotes the radical H 2 NCH(CO 2 H)CH 2 CH 2 CO- or — NHCH(CO 2 H)CH 2 CH 2 CO— according to the context, the carbon atoms in these radicals being numbered from the carbon atom of the ⁇ -carboxy group as position 1.
  • a cyclopenta[g]quinazoline of the invention contains at least two asymmetric carbon atoms [present at the point of attachment of the group — N(R 2 )— to the tricyclic ring system and at the ⁇ -carbon atom of the group — CONHCH(CO H)— ] and can therefore exist in racemic and optically active forms.
  • this invention encompasses both racemic and optically active forms of the physiologically active cyclopenta[g]quinazolines, it being a matter of common general knowledge how such optically active forms may be obtained by stereospecific synthesis or by separation of a mixture of isomeric compounds. It will be appreciated that one isomer may be of more interest than another due to the nature of the activity which it exhibits or due to superior physical properties, for example aqueous solubility.
  • a cyclopenta[g]quinazoline of the formula (I) may exhibit the phenomenon of tautomerism and that the formulae shown in this specification represent only one of the possible tautomeric forms.
  • Y 1 , Y 2 , Y 4 or Y 6 is a tetrazol-5-yl group
  • that group may be in the form of a lH-tetrazol-5-yl group or a 2H-tetrazol-5-yl group. It is to be understood therefore that the invention is not limited merely to any one tautomeric form which is illustrated.
  • certain cyclopenta[g]quinazolines of the formula (I) can exist in solvated as well as unsolvated forms such as, for example, hydrated forms.
  • a suitable value for R°, R 1 or R 2 when it is d- 4 alkyl, or for a d- 4 alkyl substituent which may be present on Ar 1 , Ar 2 or Ar or on a phenyl group-containing group Y 1 , Y 2 , Y 4 or Y 6 present in R 3 , or for a group R present in R 3 when it is d- 4 alkyl, is, for example, methyl, ethyl, propyl or isopropyl.
  • a suitable value for a C 1 - 4 alkoxy substituent which may be present on Ar 1 , Ar 2 or Ar 3 or on a phenyl-containing group Y , Y , Y 4 or Y 6 is, for example, methoxy, ethoxy, propoxy, isopropoxy or butoxy.
  • a suitable value for R°, R 1 and R 2 when it is C 3 -4 alkenyl or for a group R present in R 3 when it is alkenyl is, for example, prop-2-enyl, but-2-enyl, but-3-enyl or 2-methylprop-2-enyl; and when it is C 3 - 4 alkynyl is, for example, prop-2-ynyl or but-3-ynyl.
  • a suitable value for R°, R 1 and R 2 when it is C 2 - 4 hydroxyalkyl is, for example, 2-hydroxyethyl or 3-hydroxypropyl; when it is C 2 - 4 halogenoalkyl is, for example, 2-fluoroethyl, 2-chloroethyl, 2-bromoethyl, 3-fiuoropropyl, 3-chloropropyl or 3-bromopropyl; and when it is C 1 - 4 cyanoalkyl is, for example, cyanomethyl,
  • a suitable value for Ar 1 , Ar 2 or Ar 3 when it is phenylene is, for example, 1,3- or 1,4-phenylene, especially 1,4-phenylene.
  • a suitable value for Ar 1 , Ar 2 or Ar 3 when it is thiophenediyl is, for example, thiophene-2,4-diyl or thiophene-2,5-diyl; when it is thiazolediyl is, for example thiazole-2,4-diyl or thiazole-2,5-diyl; when it is pyridinediyl is, for example, pyridine-2,4-diyl, pyridine-2,5-diyl, pyridine-2,6-diyl or pyridine-3,5-diyl; and when it is pyrimidinediyl is, for example, pyrimidine-2,4-diyl, pyrimidine-2,5-diyl or pyrimidine-4,6-diyl.
  • Ar 1 and a phenylene group Ar 2 or Ar 3 or a phenyl group in Y 1 may carry one or two substituents.
  • a preferred level of substitution in Ar 1 is either two substituents or especially one substituent; and the one or two substituents may conveniently be at positions adjacent to the atom bonded to the group — CONHCH(CO 2 H)— R 3 , halogeno substituents such as fluoro being preferred.
  • a preferred level of substitution on a phenylene group Ar 2 or Ar 3 or on a phenyl group in Y 1 , where substitution is present, is one substituent.
  • R 3 is a group of the formula:
  • a suitable value for A 1 when it is a d_ 2 alkylene group is, for example methylene or ethylene and for A 2 when it is a d_ 3 alkylene group is, for example, methylene, ethylene or trimethylene.
  • a preferred value for both A 1 , when it is not a bond, and for A 2 is methylene or ethylene.
  • Suitable values for Ar 2 include those which have been discussed hereinbefore, such as thiophenediyl or most especially phenylene, or additionally tetrazole-l,5-diyl or tetrazole-2,5-diyl.
  • a suitable value for Y 1 or for Y 2 in a group Y 1 of formula -CON(R)CH(Y 2 )Y 3 when it is N-(d-4 alkylsulfonyI)carbamoyl is, for example, N-methylsulfonylcarbamoyl, N-ethyl- sulfonylcarbamoyl or N-propylsulfonylcarbamoyl.
  • a suitable value for A 4 when Y 3 is a group of the formula — A 4 — CO 2 H is trimethylene, pentamethylene or hexamethylene, A 4 preferably being a C 3 _ ⁇ 5 alkylene group with especially suitable values for Y 3 being — (CH 2 ) ⁇ CO 2 H where n is 3, 4 or 5.
  • a preferred value for Y 1 or for Y 2 , Y 4 and Y 6 is tetrazol-5-yl or especially carboxy.
  • R 3 is a group of the formula:
  • a suitable value for A 5 is, for example, methylene, ethylene, trimethylene, tetramethylene, pentamethylene or hexamethylene and a suitable value for R is as described hereinbefore.
  • a suitable value for Y 4 when it is N-(C ⁇ -4 alkylsulfonyl)- carbamoyl is, for example, N-methylsulfonylcarbamoyl, N-ethylsulfonylcarbamoyl or N-propylsulfonylcarbamoyl.
  • Y 5 is a group of the formula -A 4 -CO 2 H
  • suitable values for A 4 and Y are as described hereinbefore in relation to a group Y 3 of the formula -A 4 -CO 2 H.
  • a suitable value for A 6 in a group Y 5 of the formula — A 6 — Ar 3 — A 7 — Y 6 is as described hereinbefore for A 1 and for A 7 is as described for A 2 .
  • a suitable value for Ar 3 is as described hereinbefore for Ar 2 .
  • a suitable value for Y 6 in such a group Y when it is N-(C ⁇ ⁇ alkylsulfonyl)carbamoyl is, for example, N-methylsulfonylcarbamoyl, N-ethylsulfonylcarbamoyl or N-propylsulfonylcarbamoyl.
  • R 3 is a group of the formula:
  • a suitable value for A is, for example, methylene, ethylene, trimethylene or tetramethylene.
  • Groups R 3 of particular value have the formula — A 1 — Ar 2 — A 2 — Y 1 , especially when Y 1 is a group not of the formula — CO ⁇ (R)CH(Y 2 )Y 3 , such as carboxy or tetrazol-5-yl.
  • Specific examples of such groups R 3 are groups — A 1 — Ar 2 — A 2 — Y in which A is a bond or methylene or ethylene, Ar is phenylene, thiophenediyl or tetrazolediyl, A 2 is methylene, ethylene or trimethylene and Y 1 is carboxy or tetrazol- 5-yl.
  • Other groups R 3 of particular value have the formula — A 1 — Ar 2 — A 2 — Y 1 , especially when Y 1 is a group not of the formula — CO ⁇ (R)CH(Y 2 )Y 3 , such as carboxy or tetrazol-5-yl.
  • Y 5 is a group of the formula — A 6 — Ar 3 — A 7 — Y 6 in which Ar 3 is phenylene, thiophenediyl or tetrazolediyl.
  • a 5 is methylene or ethylene
  • R is hydrogen or methyl
  • Y 4 is carboxy or tetrazol-5-yl
  • a 6 is a bond or is methylene or ethylene
  • Ar 3 is phenylene, thiophenediyl or tetrazolediyl
  • a 7 is methylene, ethylene or trimethylene and Y 6 is carboxy or tetrazol-5-yl.
  • a suitable pharmaceutically-acceptable salt form of a cyclopenta[g]- quinazoline of the invention is, for example, an acid addition salt with an inorganic or organic acid, for example hydrochloric, hydrobromic, trifluoroacetic or maleic acid; or an alkali metal, for example sodium, an alkaline earth metal, for example calcium, or ammonium, for example tetra(2-hydroxyethyl)ammonium, salt.
  • a suitable pharmaceutically-acceptable ester form of a cyclopentafg]- quinazoline of the invention is, for example, an ester with an aliphatic alcohol of up to 6 carbon atoms, for example a methyl, ethyl or tert-butyl ester.
  • R 3 may contain several carboxy groups in addition to the carboxy group in the grouping — CONHCH(CO 2 H)— .
  • a salt or ester may be mono-acid-mono-salt or -ester, di-salt or di-ester and when, for example, three carboxy groups are present a salt or ester may be mono-acid-di-salt or -ester, di-acid-morio-salt or -ester or even tri-salt or -ester.
  • a preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein R° and R 1 are each independently hydrogen or d- 4 alkyl, especially methyl; wherein R 2 is ethyl, propyl, prop-2-enyl, prop-2-ynyl, 2-hydroxyethyl, 2- fluoroethyl, 2-bromoethyl or 2-cyanoethyl; wherein Ar 1 is 1,4-phenylene which may optionally bear one or two substituents selected from the group consisting of chloro and especially fluoro, thiophene-2,5-diyl, thiazole-2,5-diyl or pyridine-2,5-diyl; wherein R 3 is a group of the formula — A 1 — Ar 2 — A 2 —
  • R is hydrogen, d-4 alkyl, C 3 -4 alkenyl or C 3 - 4 alkynyl;
  • Y 2 is carboxy, tetrazol-5-yl, alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d- 4 alkyl and d- 4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; and Y 3 is the residue of a naturally occurring amino acid ⁇ H CH(CO 2 H)Y 3 .
  • a preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein:
  • R 2 is ethyl, propyl, prop-2-enyl, prop-2-ynyl, 2-hydroxyethyl, 2-fluoroethyl, 2-bromoethyl or 2-cyanoethyl;
  • Ar 1 is 1,4-phenylene which may optionally bear one or two substituents selected from the group consisting of chloro, fluoro, thiophene-2,5-diyl, thiazole-2,5- -diyl or pyridine-2,5-diyl;
  • R 3 is a group of the formula:
  • a 5 is a d- 5 alkylene group and R is as defined above;
  • Y 4 is carboxy, tetrazol-5-yl, N-(d-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C 1 - 4 alkyl and C 1 - 4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; and
  • Y 5 is the residue of a naturally occurring amino acid ⁇ H 2 CH(CO 2 H)Y 5 .
  • a preferred value for p is 1.
  • a further preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein A is a group OR 0 in which R is hydrogen or methyl; wherein R 2 is ethyl or prop-2-ynyl; and wherein Ar 1 is 1,4-phenylene or 1,4-phenylene having a 2-fluoro substituent as in 2,6-difluoro- 1,4-phenylene or especially 2-fluoro- 1,4-phenylene or is pyridine 2,5-diyl; and
  • quinazolines of the invention of particular interest have the values of R°, R 1 , R 2 , and Ar 1 and Ar in combination as indicated above but with R 3 having any value as indicated hereinbefore.
  • specific particularly preferred cyclopenta- g] quinazolines of the invention are:
  • the asymmetric carbon atom of a residue R 3 of the form -A 1 -Ar 2 -A 2 -CON(R)CH(Y 2 )Y 3 or -A 5 -CON(R)CH(Y 4 )Y 5 may be of the L- or D-configuration but the amide bond will be stabilised in vivo when it is of the D-configuration as it will also be when R is other than hydrogen.
  • Y 3 or Y 5 is the residue of a naturally occurring amino acid, however, the amino acid intermediate for the synthesis of the cyclopenta[g]quinazoline will of course be more readily available when this asymmetric carbon atom is of the L-configuration.
  • a cyclopenta[g]quinazoline of the invention may be prepared by any process known to be applicable to the preparation of chemically-related compounds.
  • cyclopenta[g] quinazolines of the present invention are believed to function as anti-cancer agents at least in part due to their ability to inhibit the enzyme thymidylate synthase.
  • This anti-cancer activity may be assessed, for example, using one or more of the procedures set out below:
  • Thymidylate synthase may be obtained in partially purified form from L1210 mouse leukaemia cells and utilised in the assay using the procedures described by Jackman et al. (Cancer Res., 1986, 46, 2810) and Sikora et ⁇ /. (Biochem. Pharmacol, 1988, 37, 4047);
  • Test (a) IC 50 in the range, for example, 0.0001-1 ⁇ M;
  • Test (c) IC 50 in the range, for example, 0.001-10 ⁇ M;
  • Test (d) IC 50 in the range, for example, 0.01-100 ⁇ M;
  • a cyclopenta[g]quinazoline of the present invention may itself be active or it may be a pro-drug which is converted in vivo to an active compound.
  • a cyclopenta[g]quinazoline of the invention may be administered to a warm-blooded animal, including a human, in the form of a pharmaceutical composition which comprises the cyclopenta[g]quinazoline in association with a pharmaceutically- acceptable diluent or carrier.
  • the composition may be in a form suitable for oral use, for example a tablet, capsule, aqueous or oily solution, suspension or emulsion; a form suitable for topical use, for example a cream, ointment, gel or aqueous or oily solution or suspension; a form suitable for nasal use, for example a snuff, nasal spray or nasal drops; a form suitable for vaginal or rectal use, for example a suppository; a form suitable for administration by inhalation, for example as a finely divided powder such as a dry powder, a microcrystalline form or a liquid aerosol; a form suitable for sub-lingual or buccal use, for example a tablet or capsule; or a form suitable for parenteral use (including intravenous, subcutaneous, intramuscular, intravascular or infusion use), for example a sterile aqueous or oily solution, emulsion or suspension.
  • the above compositions may be prepared in a conventional manner using conventional excipients.
  • composition may contain, in addition to the cyclopenta[g]quinazoline of the invention, one or more other anti-cancer substances selected from, for example, other antimetabolites, DNA interacting agents, signal transduction inhibitors or other inhibitors of deregulated pathways in tumours.
  • other anti-cancer substances selected from, for example, other antimetabolites, DNA interacting agents, signal transduction inhibitors or other inhibitors of deregulated pathways in tumours.
  • the cyclopenta[g]quinazoline will normally be administered to a warmblooded animal at a dose within a range of 50-25000, particularly 50-5000, mg per square metre body area of the animal, i.e. approximately 1500, particularly 1-100, mg/kg. Where desired, however, dosages outside this range may be employed and, in particular, where the preferred mode of administration involving subcutaneous infusion is used then the does range may be increased to 1-1000 mg/kg. Preferably a daily dose in the range 10-250 mg/kg is employed, particularly 30-150 mg/kg. However, the daily dose will necessarily be varied depending upon the host treated, the particular route of administration and the severity of the illness being treated. Accordingly, the optimum dosage may be determined by the practitioner who is treating any particular patient.
  • the present invention also includes a method for aiding regression and palliation of cancer in a patient, particularly a warm-blooded animal such as a human, in need of such treatment, which comprises administering to said patient an effective amount of a cyclopenta[g]quinazoline as defined hereinbefore.
  • the invention also provides the use of such a cyclopenta[g]quinazoline in the manufacture of a novel medicament for use in the treatment of cancer.
  • Cyclopenta[g]quinazolines of the present invention are of interest for a wide range of anti-tumour activities particularly the treatment of ovarian cancer.
  • the cyclopenta[g]quinazolines of the present invention are also of interest for use in the treatment of other conditions, for example allergic conditions such as psoriasis and inflammatory diseases such as rheumatoid arthritis.
  • allergic conditions such as psoriasis and inflammatory diseases such as rheumatoid arthritis.
  • the compound will normally be administered at a dose within the range 5- 25000, particularly 5-500, mg per square metre body area of the animal, i.e. approximately 0.1-500, particularly 0.1-10, mg/kg. Where desired, however, dosages outside this range may be employed.
  • compositions containing the quinazolines may be formulated in unit dosage form, i.e. in the form of discrete portions each comprising a unit dose, or a multiple or sub-multiple of a unit dose, for example as a tablet or capsule.
  • a unit dosage form may, for example, contain an amount of the cyclopenta[g]quinazoline in the range of 1-250 or 1-500 mg.
  • the black reaction mixture was stirred at room temperature for 1 hour under argon; then it was partitioned between ethyl acetate (150 ml) and saturated aqueous sodium bicarbonate (100 ml). The aqueous layer was extracted with more ethyl acetate (100 ml); the organic extracts were combined, washed with brine (100 ml), dried (Na 2 SO 4 ), and concentrated in vacuo to leave a reddish residue. Purification by column chromatography, on elution with 35%) ethyl acetate in petroleum ether (60-80 °C), afforded the desired product as a white solid: 0.175 g (31 %).
  • the brown solid was collected by filtration washed with plenty of water, then suspended in dichloromethane (100 ml). The mixture was stirred at room temperature for 10 min, dried ( ⁇ a 2 SO 4 ), and concentrated in vacuo.
  • reaction mixture was allowed to cool to room temperature, then the solvents were removed in vacuo and the residue was suspended in water ( ⁇ 15 ml).
  • the pH of this mixture was adjusted to ⁇ 12 with IN NaOH (got a clear solution), then to ⁇ 4 with IN hydrochloric acid.
  • the off white precipitate was collected by filtration, washed with water, dried in vacuo over P 2 O 5 : 0.262 g (89%), m.p.
  • the solution was stirred at room temperature under argon with protection from the light for 2.5 h.
  • the solution was partitioned between ethyl acetate (25 ml) and water (25 ml).
  • the aqueous layer was extracted with ethyl acetate (2 x 20 ml).
  • the combined organic extracts were washed with 10% aqueous citric acid (2 x 30 ml), saturated aqueous ⁇ a ⁇ CO 3 (30 ml), dilute brine (30 ml), dried (Na 2 SO 4 ) and the solvent removed in vacuo.
  • the p ⁇ of the solution was adjusted to p ⁇ 12 with IM sodium hydroxide solution and stirred at room temperature for 6 h.
  • the solution was acidified to p ⁇ 4 with IM hydrochloric acid and cooled to 0°C.
  • the precipitate was collected by filtration and dried under vacuum over P 2 O 5 to yield the desired product as a pale brown solid (27 mg, 47%); m.p.
  • Method A A solution of tert-butyl 4- ⁇ N-[(6RS)-2-(2,2-dimethyl- propionyloxymethyl)-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta[g-]quinazolin-6-yl]-N- -(prop-2-ynyl)amino ⁇ benzoate (0.150 g, 0.28 mmol) in dichloromethane (2 ml) and trifluoroacetic acid (6 ml) was stirred at room temperature for 1 hour. The solvents were then removed in vacuo, and the residue was suspended in methanol (3 ml) and water (5 ml).
  • the p ⁇ was adjusted to -10 with IN ⁇ aO ⁇ (1.1 ml), and the mixture was stirred at room temperature for 4 hours.
  • the reaction mixture was diluted with water (5 ml) and the p ⁇ was adjusted to -5 with IN ⁇ C1.
  • the solid was then collected by filtration, but 1H- ⁇ MR indicated no complete removal of the pivaloyl group.
  • This solid was suspended into the filtrate and then IN NaO ⁇ (0.9 ml, 0.9 mmol) was added (p ⁇ - 12).
  • the mixture was stirred at room temperature for 3.5 hours, then more IN NaO ⁇ (0.2 ml) was added, and the mixture was stirred at room temperature for a further 0.5 hours.
  • the pH was then adjusted to -5.0 with IN HCl.
  • Method B A solution of tert-butyl 4- ⁇ N-[(6i?S)-2-hydroxymethyl-4-oxo- -3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl]-N-(prop-2-ynyl)amino ⁇ - benzoate (0.050 g, 0.11 mmol) in dichloromethane (1 ml) and trifluoroacetic acid (2.4 ml) was stirred at room temperature for 1 hour. The solvents were then removed in vacuo, and the residue was triturated with diethyl ether. The off-white precipitate was collected by filtration, and washed with ether to obtain the desired product as the trifluoroacetate salt: 0.044 g.
  • CB300945 the (RS)-C2-CH 2 OH analogue of the known cyclopenta[ ]- quinazoline, CB300638 was synthesised as in Example 1.
  • the C2-NH. was made as a comparative example.
  • the activity of this new series of cyclopenta[ ]quinazolines with C2-CH 3 ( ⁇ S-CB300638), C2-CH 2 OH (RS- CB300945) and C2-NH 2 (RS-CB300944) substitutions were compared with three other series of quinazoline derivatives in mouse L1210-FBP cells and human tumour cell lines co-expressing the RFC and ⁇ -FR.
  • the human A431-FBP cell line was transfected with the ⁇ -FR and sensitivity compared with the A431 cell line.
  • Human nasopharengeal KB cells constitutively overexpress this receptor see Bagnoli et al. Oncogene, 19, 4754 ⁇ 1763, 2000).
  • the C2-CH2OH analogues were found to have lower affinities for the ⁇ -FR of L1210-FBP cells (0.21 to 0.29) than the 2-CH 3 (0.37 to 0.54) and particularly the 2-NH2 (1.3 to 1.7) counte ⁇ arts.
  • the C2-CH 3 analogue CB300638 had a relative binding affinity of 0.57.
  • the C2-CH 2 OH analogue CB300945 had a higher affinity than predicted (0.71) and the C2-NH 2 analogue (CB300944) had one lower than predicted (0.57).
  • C2-substitution did not markedly affect binding to the ⁇ -FR.
  • a similar pattern of binding was noted for ⁇ -FR expressed by A431-FBP cells (data not shown).
  • A431-FBP and KB cells were highly sensitive to the C2-CH 3 cyclopenta[g]- quinazoline with a L-glu- ⁇ -D-glu ligand (S or RS-CB300638).
  • very little activity of the C2-NH 2 analogue (RS-CB300944) could be attributed to ⁇ -FR mediated uptake into either of these cell lines (Tables 13).
  • the C2- CH 2 OH analogue (RS-CB300945) was highly potent in A431-FBP and KB cells and more highly selective for these cells compared with A431 cells, than RS-CB300638. Tables 4 and 5 show the structures of the compounds tested.
  • Maize starch paste (5% w/v paste) 2.25
  • Citric acid 0.38% w/v
  • the above formulations may be prepared by conventional procedures well known in the pharmaceutical art.
  • the tablets (a) to (c) may be enteric coated by conventional means, for example with a coating of cellulose acetate phthalate.
  • Table 1 Activity of 2-substituted quinazolines and eye lopenta[g] quinazolines in human A431 and A431-FBP cell lines grown in 20 nM folat
  • A431 A431+l ⁇ MFA A431-FBP A431-FBP+l ⁇ MFA fold increased IC S0 in (fold increased sensitivity (fold increased lC$o in presence of folic acid) compared with A431) presence of folic acid
  • Table 2 Activity of 2-substituted quinazolines and cyclopenta[g]quinazolines in human A431 and A431-FBP cell lines grown in 1 nM folate (
  • Table 3 Activity of 2-substituted quinazolines and cyclopenta[g]quinazolines in human KB cells grown in 1 and 20 nM folate (RS LV)

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Abstract

Cyclopenta[g]quinazolines of the formula (I):- wherein: A is a group OR or NR0R1 wherein R?0 and R1¿ are each independently hydrogen C¿l-4? alkyl, C3-4 alkenyl, C3-4 alkynyl, C2-4 hydroxyalkyl, C2-4 halogenoalkyl or Cl-4 cyanoalkyl, or R?0 and R1¿ together with the intermediate N form a five- or six-membered heterocyclic ring; p is an integer in the range 1 to 4; R2 is hydrogen, C¿1-4? alkyl, C3-4 alkenyl, C3-4 alkynyl, C2-4 hydroxyalkyl, C2-4 halogenoalkyl or Cl-4 cyanoalkyl; Ar?l¿ is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, C¿l-4? alkyl and Cl-4 alkoxy; and R?3¿ is a group of one of the following formulae: -A?1-Ar2-A2-Y1 -A5¿-CON(R)CH(Y?4)Y5 -A8-X-Ar4¿ and pharmaceutically acceptable salts or esters thereof are of therapeutic value particularly in the treatment of cancer.

Description

ANTI- CANCER CYCLOPENTA QUINAZO INE COMPOUNDS
This invention relates to novel anti-cancer agents and more particularly it relates to cyclopenta[g]quinazoline derivatives which possess antiproliferative activity. One group of anti-cancer agents comprises antimetabolites having antifolate activity, such as the dihydrofolate reductase inhibitor, methotrexate and the thymidylate synthase (TS) inhibitors CB3717, raltitrexed and ZD9331. CB3717 is described and claimed in EP-B-0031237, raltitrexed in EP-B-0239362 and ZD9331 in EP-B-0562734. All of these TS inhibitors have demonstrable clinical activity in a range of solid tumours (see Cancer Treatment Reports, 1986, 70, 1335 and Beale et al., "Tomudex: Clinical Development" in Antifolate Drugs in Cancer Therapy (ed. Jackman), Humana Press, Totowa, New Jersey, USA, pp. 177-181, 1999). Side- effects of raltitrexed and ZD9331 are predominantly related to inhibition of TS in gut and bone-marrow. TS catalyses the methylation of deoxyuridine monophosphate to produce thymidine monophosphate which is required for DNA synthesis. The anticancer activity of these agents may be assessed in vitro by determining their inhibitory effect on that enzyme, and in cell cultures by their inhibitory effect on a range of mouse and human cancer cell lines (see Boyle et al, "ZD9331: Preclinical and clinical studies" in Antifolate Drugs in Cancer Therapy (ed. Jackman), Humana Press, Totowa, New Jersey, USA, pp. 243-260, 1999 and Hughes et al, "Raltitrexed (Tomudex), a highly polyglutamatable antifolate thymidylate synthase inhibitor: design and preclinical activity" in Antifolate Drugs in Cancer Therapy (ed. Jackman), Humana Press, Totowa, New Jersey, USA, pp. 147-165, 1999). More recently, cyclopenta[g]quinazoline derivatives showing a good level of activity both as regards their ability to inhibit TS and also as regards their anticancer activity against various cell lines have been developed.
WO-A-94/11354 (British Technology Group Limited) discloses tricyclic compound of formula:
Figure imgf000003_0001
wherein R1 is hydrogen, amino, d-4 alkyl, d- alkoxy, C1-4 hydroxyalkyl or - fluoroalkyl;
R2 is hydrogen, Cι_4 alkyl, C3-4 alkenyl, C3-4 alkynyl, C2--4 hydroxyalkyl C2-4 halogenoalkyl or C1- cyanoalkyl;
Ar is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, d- alkyl and C\-A alkoxy; and
R is a group of one of the following formulae:
-NHCH(CO2H) -A 1'-Yl1 -NH-A . 3J-Yr3
or R3 is a N-linked naturally-occurring amino acid selected from the group consisting of L-alanine, L-leucine, L-isoleucine, L-valine and L-phenylalanine. Among the compounds disclosed is the L-Glu-γ-D-Glu compound CB300638, also mentioned in Clinical Cancer Research, 5, November 1999 (Supplement) at #566 (Theti et al.) and Proceedings of the American Association for Cancer Research, 41, March 2000 at #33 (Jackman et al), as well as in J. Med. Chem., 2000, 43, 1910-1926, where it is disclosed on page 1923 as compound 7b. WO-A-95/30673 (British Technology Group Limited) discloses cyclopenta-
[gjquinazo lines of formula:
Figure imgf000003_0002
wherein R1 is hydrogen, amino, C1-4 alkyl, - alkoxy, C1-4 hydroxyalkyl or Cι_4 fluoroalkyl; R2 is hydrogen, C1-4 alkyl, C3- alkenyl, C^ alkynyl, C2-4 hydroxyalkyl, C2_4 halogenoalkyl or C1-4 cyanoalkyl;
Ar1 is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, C1-4 alkyl and d-4 alkoxy; and
R3 is a group of one of the following formulae:
-A'-Ai^-A^Y1 -A5-CON(R)CH(Y4)Y5 -A8-X-Ar4
The α-isoform of the folate receptor (α-FR; membrane-associated fo late- binding protein) is a glycosylphosphatidylinositol anchored cell membrane protein that has very high affinity for folic acid and the more biologically relevant reduced- -folates (Kd ~0.1 nM). The mechanism of folate internalisation is receptor-mediated endocytosis. The α-FR is overexpressed in many carcinomas, particularly those of ovarian origin where it is overexpressed highly and homogeneously in 90% of cases; see Cancer Res. 51, 5329-5338, 1991 (Campbell et al, 1991). Furthermore, high α-FR expression has been linked to aggressive, platinum resistant disease and poor prognosis— see Int. J. Cancer 74, 193-198, 1997 and Int. J. Cancer 79, 121-126, 1998 (both Toffoli et al). The β-isoform is widely expressed in tumours of epithelial and non-epithelial origin with expression levels being generally low/moderate and high, respectively, reviewed in Critical Rev. Therap. in Drug Carrier Systems 15, 587-627, 1998 (Reddy and Low).
Folate receptors (α and β) are expressed in some adult normal tissues (low to moderate expression). Significant expression of the α-FR is largely restricted to kidney proximal tubules and choroid plexus although it is suggested that it is localised to the apical membrane surface in these organs and therefore may not play a significant role in folate uptake from blood (Reddy and Low, ibid.). There may be a specialised function of the α-FR in the proximal tubules of the kidney to salvage folates that escape in the filtrate. The α-FR is hypothesised to be involved in cell signalling pathways. For example, in IGROV-1 ovarian carcinoma cells, immunoprecipitation experiments have shown that the α-FR is associated in membranes with the G protein Gαι-3, and the non-receptor kinase lyn. High FR expression in some tumours relative to normal tissues is being exploited in several areas of cancer medicine, including the selective tumour delivery of conjugates of folic acid and toxins, liposomes, imaging or cytotoxic agents (Reddy and Low, ibid.). For example, folic acid-deferroxamine-u lIn conjugates are detected only in FR-expressing tumours and not normal tissues of mice, with the exception of kidney epithelial cells. The high selectivity of this approach resides in the very low and high affinities of folic acid (not a major component of plasma) for the RFC (reduced-folate carrier) and FR respectively. Thus antifolate drugs with similarly low and high affinity for the RFC and α-FR respectively could be highly selective for α- FR over-expressing tumours relative to normal tissues. In contrast with the folic acid conjugates they would not require intracellular cleavage to be active.
We have now discovered that certain compounds within the general class of cyclopenta[g]quinazolines have an unexpectedly high level of selectivity for α-folate receptor expressing human tumour cell lines. Accordingly the present invention comprises a cyclopenta[g] quinazoline of formula (I) :
Figure imgf000005_0001
(I) wherein:
A is a group OR0 or NR^1 wherein R° and R1 are each independently hydrogen, d- alkyl, C3-4 alkenyl, C3_4 alkynyl, C2-4 hydroxyalkyl, C2- halogenoalkyl or d- cyanoalkyl, or R° and R1 together with the intermediate N form a five- or six-membered heterocyclic ring; p is an integer in the range 1 to 4;
R2 is hydrogen, C1-4 alkyl, C3-4 alkenyl, C3-4 alkynyl, C2- hydroxyalkyl, C2-4 halogenoalkyl or C1-4 cyanoalkyl;
Ar1 is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, C1-4 alkyl and C1-4 alkoxy; and R3 is a group of the formula: -A'-Ar^-Y1
in which A 1 is a bond between the α-carbon atom of the group -CO-NH-CH(CO2H)- and Ar2 or is a C ι_2 alkylene group;
Ar is phenylene, tetrazolediyl, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which in the case of phenylene may optionally bear one or two substituents on the ring selected from halogeno, nitro, d-4 alkyl and d- alkoxy;
A2 is a d-.3 alkylene or C2_3 alkenylene group; and Y1 is carboxyl, tetrazol-5-yl, N-(Cι-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d-4 alkyl and -4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; or
Y1 is a group of the formula:
-COΝ(R)CH(Y2)Y3
in which R is hydrogen, C1-4 alkyl, C3_4 alkenyl or C3-4 alkynyl;
Y2 is carboxy, tetrazol-5-yl, N-(d_4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfmyl or tetrazol-5-ylsulfonyl; and
Y3 is the residue of a naturally occurring amino acid ΝH2CH(CO2H)Y3; or
Y3 is a group of the formula:
-A4-CO2H
in which A4 is a C2_6 alkylene group; R is a group of the formula:
-A5-CON(R)CH(Y4)Y5
in which A5 is a Cι_6 alkylene group and R is as defined above; Y4 is carboxy, tetrazol-5-yl, N-(Cι-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfιnyl or tetrazol-5-ylsulfonyl; and Y5 is the residue of a naturally occurring amino acid ΝH2CH(CO2H)Y5; or
Y5 is a group of the formula:
-A4-CO2H
in which A4 is as defined above; or
Y5 is a group of the formula:
-A6-Ar3-A7-Y6
in which A6 is a bond between the α-carbon atom of the group
-A5-CON(R)CH(YV and Ar3 or is a d_2 alkylene group;
Ar3 is phenylene, tetrazolediyl, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which in the case of phenylene may optionally bear one or two substituents on the ring selected from halogeno, nitro, d-4 alkyl and d-4 alkoxy; A7 is a Cι_3 alkylene or C2_3 alkenylene group; and
Y6 is carboxy, tetrazol-5-yl, N-(Cι_4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C1-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfιnyl or tetrazol-5-ylsulfonyl; or R3 is a group of the formula:
-A8-X-Ar4
in which A is a C1-4 alkylene group; X is sullϊnyl, sulfonyl or methylene; and
Ar4 is l,2,3-triazol-4-yl, l,2,4-triazol-3-yl or, except when X is methylene, tetrazol-5-yl; the compound (I) optionally being in the form of a pharmaceutically acceptable salt or ester. In this specification the terms alkyl, alkenyl, alkynyl and alkylene include both straight and branched chain groups but references to individual alkyl or alkylene groups, such as "propyl", are specific for the straight chain group only. An analogous convention applies to other generic terms. Moreover, the numbering system used for the cyclopenta[g]quinazoline nucleus is the conventional one as shown below:
Figure imgf000008_0001
Amino-acid residues are designated herein in the standard manner (Pure and Applied Chemistry, 1974, 40, 317 and European Journal of Biochemistry, 1984, 138, 9). Thus, for example, γ-glutamyl denotes the radical H2NCH(CO2H)CH2CH2CO- or — NHCH(CO2H)CH2CH2CO— according to the context, the carbon atoms in these radicals being numbered from the carbon atom of the α-carboxy group as position 1.
It will be observed that a cyclopenta[g]quinazoline of the invention contains at least two asymmetric carbon atoms [present at the point of attachment of the group — N(R2)— to the tricyclic ring system and at the α-carbon atom of the group — CONHCH(CO H)— ] and can therefore exist in racemic and optically active forms. It is to be understood that this invention encompasses both racemic and optically active forms of the physiologically active cyclopenta[g]quinazolines, it being a matter of common general knowledge how such optically active forms may be obtained by stereospecific synthesis or by separation of a mixture of isomeric compounds. It will be appreciated that one isomer may be of more interest than another due to the nature of the activity which it exhibits or due to superior physical properties, for example aqueous solubility.
It is also to be understood that a cyclopenta[g]quinazoline of the formula (I) may exhibit the phenomenon of tautomerism and that the formulae shown in this specification represent only one of the possible tautomeric forms. Moreover, it will be appreciated that when, for example, Y1, Y2, Y4 or Y6 is a tetrazol-5-yl group, that group may be in the form of a lH-tetrazol-5-yl group or a 2H-tetrazol-5-yl group. It is to be understood therefore that the invention is not limited merely to any one tautomeric form which is illustrated. It is also to be understood that certain cyclopenta[g]quinazolines of the formula (I) can exist in solvated as well as unsolvated forms such as, for example, hydrated forms.
A suitable value for R°, R1 or R2 when it is d-4 alkyl, or for a d-4 alkyl substituent which may be present on Ar1, Ar2 or Ar or on a phenyl group-containing group Y1, Y2, Y4 or Y6 present in R3, or for a group R present in R3 when it is d-4 alkyl, is, for example, methyl, ethyl, propyl or isopropyl.
A suitable value for a C1-4 alkoxy substituent which may be present on Ar1, Ar2 or Ar3 or on a phenyl-containing group Y , Y , Y4 or Y6 is, for example, methoxy, ethoxy, propoxy, isopropoxy or butoxy.
A suitable value for a halogeno substituent which may be present on Ar1, Ar2 or Ar3 or on a phenyl-containing group Y1, Y2, Y4 or Y6 is, for example, fluoro, chloro or bromo.
A suitable value for R°, R1 and R2 when it is C3-4 alkenyl or for a group R present in R3 when it is alkenyl, is, for example, prop-2-enyl, but-2-enyl, but-3-enyl or 2-methylprop-2-enyl; and when it is C3-4 alkynyl is, for example, prop-2-ynyl or but-3-ynyl.
A suitable value for R°, R1 and R2 when it is C2-4 hydroxyalkyl is, for example, 2-hydroxyethyl or 3-hydroxypropyl; when it is C2-4 halogenoalkyl is, for example, 2-fluoroethyl, 2-chloroethyl, 2-bromoethyl, 3-fiuoropropyl, 3-chloropropyl or 3-bromopropyl; and when it is C1-4 cyanoalkyl is, for example, cyanomethyl,
2-cyanoethyl or 3-cyanopropyl.
When R° and R1 together with the intermediate N form a five- or six- -membered heterocyclic ring, this may bear substituents, but the ring is preferably an unsubstituted saturated ring such as pyrrolidine or piperidine.
A suitable value for Ar1, Ar2 or Ar3 when it is phenylene is, for example, 1,3- or 1,4-phenylene, especially 1,4-phenylene.
A suitable value for Ar1, Ar2 or Ar3 when it is thiophenediyl is, for example, thiophene-2,4-diyl or thiophene-2,5-diyl; when it is thiazolediyl is, for example thiazole-2,4-diyl or thiazole-2,5-diyl; when it is pyridinediyl is, for example, pyridine-2,4-diyl, pyridine-2,5-diyl, pyridine-2,6-diyl or pyridine-3,5-diyl; and when it is pyrimidinediyl is, for example, pyrimidine-2,4-diyl, pyrimidine-2,5-diyl or pyrimidine-4,6-diyl. As indicated, Ar1 and a phenylene group Ar2 or Ar3 or a phenyl group in Y1 may carry one or two substituents. A preferred level of substitution in Ar1, where substitution is present, is either two substituents or especially one substituent; and the one or two substituents may conveniently be at positions adjacent to the atom bonded to the group — CONHCH(CO2H)— R3, halogeno substituents such as fluoro being preferred. A preferred level of substitution on a phenylene group Ar2 or Ar3 or on a phenyl group in Y1, where substitution is present, is one substituent.
When R3 is a group of the formula:
Figure imgf000010_0001
a suitable value for A1 when it is a d_2 alkylene group is, for example methylene or ethylene and for A2 when it is a d_3 alkylene group is, for example, methylene, ethylene or trimethylene. A suitable value for A2 when it is a C _3 alkenylene group is, for example, vinylene or especially propenylene (-CH2CH=CH- or -CH=CH-CH2-). A preferred value for both A1, when it is not a bond, and for A2 is methylene or ethylene. Suitable values for Ar2 include those which have been discussed hereinbefore, such as thiophenediyl or most especially phenylene, or additionally tetrazole-l,5-diyl or tetrazole-2,5-diyl. A suitable value for Y1 or for Y2 in a group Y1 of formula -CON(R)CH(Y2)Y3 when it is N-(d-4 alkylsulfonyI)carbamoyl is, for example, N-methylsulfonylcarbamoyl, N-ethyl- sulfonylcarbamoyl or N-propylsulfonylcarbamoyl.
In a group Y1 of formula -COΝ(R)CH(Y2)Y3 suitable values for d- alkyl, C3-4 alkenyl and C3-4 alkynyl groups R have been discussed hereinbefore but R is preferably either methyl or especially hydrogen; a suitable value for Y when it is the residue of a naturally occurring amino acid is the residue of alanine (Y3 = CH3), arginine (Y3 = (CH2)3NHC(NH2)=NH), aspartic acid (Y3 = CH2CO2H), cysteine (Y3 - CH2SH), isoleucine (Y3 - CH(CH3)CH2CH3), leucine (Y3 = CH2CH(CH3)CH3), ornithine (Y3 = (CH2)3NH2), phenylalanine (Y3 = CH2C6H5), serine (Y3 = CH2OH) and valine (Y3 - CH(CH3)2) and especially glutamic acid (Y3 = CH2CH2CO2H). A suitable value for A4 when Y3 is a group of the formula — A4— CO2H is trimethylene, pentamethylene or hexamethylene, A4 preferably being a C3_<5 alkylene group with especially suitable values for Y3 being — (CH2)πCO2H where n is 3, 4 or 5. A preferred value for Y1 or for Y2, Y4 and Y6, is tetrazol-5-yl or especially carboxy.
When R3 is a group of the formula:
-A5-CON(R)CH(Y4)Y5
a suitable value for A5 is, for example, methylene, ethylene, trimethylene, tetramethylene, pentamethylene or hexamethylene and a suitable value for R is as described hereinbefore. A suitable value for Y4 when it is N-(Cι-4 alkylsulfonyl)- carbamoyl is, for example, N-methylsulfonylcarbamoyl, N-ethylsulfonylcarbamoyl or N-propylsulfonylcarbamoyl.
A suitable value for Y5 when it is the residue of a naturally occurring amino acid is alanine (Y5 - CH3), arginine (Y5 = (CH2)3ΝHC(ΝH2)=ΝH), aspartic acid (Y5 = CH2CO2H), cysteine (Y5 = CH2SH), isoleucine (Y5 = CH(CH3)CH2CH3), leucine (Y5 = CH2CH(CH3)CH3), ornithine (Y5 = (CH2)3NH2), phenylalanine (Y5 = CH2C6H5), serine (Y5 = CH2OH). valine (Y5 = CH(CH3)2), and especially glutamic acid (Y5 - CH2CH2CO2H). When Y5 is a group of the formula -A4-CO2H, suitable values for A4 and Y are as described hereinbefore in relation to a group Y3 of the formula -A4-CO2H. A suitable value for A6 in a group Y5 of the formula — A6— Ar3— A7— Y6 is as described hereinbefore for A1 and for A7 is as described for A2. A suitable value for Ar3 is as described hereinbefore for Ar2. A suitable value for Y6 in such a group Y when it is N-(C\^ alkylsulfonyl)carbamoyl is, for example, N-methylsulfonylcarbamoyl, N-ethylsulfonylcarbamoyl or N-propylsulfonylcarbamoyl. When R3 is a group of the formula:
-A8-X-Ar4
a suitable value for A is, for example, methylene, ethylene, trimethylene or tetramethylene.
Groups R3 of particular value have the formula — A1— Ar2— A2— Y1, especially when Y1 is a group not of the formula — COΝ(R)CH(Y2)Y3, such as carboxy or tetrazol-5-yl. Specific examples of such groups R3 are groups — A1— Ar2— A2— Y in which A is a bond or methylene or ethylene, Ar is phenylene, thiophenediyl or tetrazolediyl, A2 is methylene, ethylene or trimethylene and Y1 is carboxy or tetrazol- 5-yl. Other groups R3 of particular value have the formula
-A5-CON(R)CH(Y4)Y5, especially when Y5 is a group of the formula — A6— Ar3— A7— Y6 in which Ar3 is phenylene, thiophenediyl or tetrazolediyl.
Specific examples of such groups R3 are groups
-A5-CON(R)CH(Y4)-A6-Ar3-A7-Y0 in which A5 is methylene or ethylene, R is hydrogen or methyl, Y4 is carboxy or tetrazol-5-yl, A6 is a bond or is methylene or ethylene, Ar3 is phenylene, thiophenediyl or tetrazolediyl, A7 is methylene, ethylene or trimethylene and Y6 is carboxy or tetrazol-5-yl.
A suitable pharmaceutically-acceptable salt form of a cyclopenta[g]- quinazoline of the invention is, for example, an acid addition salt with an inorganic or organic acid, for example hydrochloric, hydrobromic, trifluoroacetic or maleic acid; or an alkali metal, for example sodium, an alkaline earth metal, for example calcium, or ammonium, for example tetra(2-hydroxyethyl)ammonium, salt.
A suitable pharmaceutically-acceptable ester form of a cyclopentafg]- quinazoline of the invention is, for example, an ester with an aliphatic alcohol of up to 6 carbon atoms, for example a methyl, ethyl or tert-butyl ester.
It is to be understood that R3 may contain several carboxy groups in addition to the carboxy group in the grouping — CONHCH(CO2H)— . When, for example, two carboxy groups are present in the cyclopenta[g]quinazoline, a salt or ester may be mono-acid-mono-salt or -ester, di-salt or di-ester and when, for example, three carboxy groups are present a salt or ester may be mono-acid-di-salt or -ester, di-acid-morio-salt or -ester or even tri-salt or -ester.
Particularly preferred values for the various symbols R°, R1, R2 and Ar1 individually are as expressed for the preferred cyclopenta[g]quinazolines described hereinafter. A preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein R° and R1 are each independently hydrogen or d-4 alkyl, especially methyl; wherein R2 is ethyl, propyl, prop-2-enyl, prop-2-ynyl, 2-hydroxyethyl, 2- fluoroethyl, 2-bromoethyl or 2-cyanoethyl; wherein Ar1 is 1,4-phenylene which may optionally bear one or two substituents selected from the group consisting of chloro and especially fluoro, thiophene-2,5-diyl, thiazole-2,5-diyl or pyridine-2,5-diyl; wherein R3 is a group of the formula — A1— Ar2— A2— Y1 in which A1 is a bond or is methylene oor ethylene, Ar is phenylene, A is methylene, ethylene or trimethylene and Y1 is a group of the formula
-CON(R)CH(Y2)Y3
in which R is hydrogen, d-4 alkyl, C3-4 alkenyl or C3-4 alkynyl;
Y2 is carboxy, tetrazol-5-yl,
Figure imgf000013_0001
alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d-4 alkyl and d-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; and Y3 is the residue of a naturally occurring amino acid ΝH CH(CO2H)Y3.
Alternatively, a preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein:
R° and R1 are each independently hydrogen or d-4 alkyl;
R2 is ethyl, propyl, prop-2-enyl, prop-2-ynyl, 2-hydroxyethyl, 2-fluoroethyl, 2-bromoethyl or 2-cyanoethyl;
Ar1 is 1,4-phenylene which may optionally bear one or two substituents selected from the group consisting of chloro, fluoro, thiophene-2,5-diyl, thiazole-2,5- -diyl or pyridine-2,5-diyl;
R3 is a group of the formula:
-A5-CON(R)CH(Y4)Y5
in which A5 is a d-5 alkylene group and R is as defined above; Y4 is carboxy, tetrazol-5-yl, N-(d-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C1-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; and
Y5 is the residue of a naturally occurring amino acid ΝH2CH(CO2H)Y5. A preferred value for p is 1. A further preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein A is a group OR0 in which R is hydrogen or methyl; wherein R2 is ethyl or prop-2-ynyl; and wherein Ar1 is 1,4-phenylene or 1,4-phenylene having a 2-fluoro substituent as in 2,6-difluoro- 1,4-phenylene or especially 2-fluoro- 1,4-phenylene or is pyridine 2,5-diyl; and
R is as just described above.
An especially preferred cyclopenta[g]quinazoline of the invention has the formula (I) wherein A is a group OR0 in which R° is hydrogen or methyl; wherein R2 is ethyl or preferably prop-2-ynyl; wherein Ar1 is 1,4-phenylene or 2-fluoro- 1,4-phenylene; and wherein R3 is the residue of an acid NH2.CH(COOH)R3 which comprises L-Glu-γ-D-Glu, i.e. N-L-γ-glutamyl-D-glutamic acid.
Other quinazolines of the invention of particular interest have the values of R°, R1, R2, and Ar1 and Ar in combination as indicated above but with R3 having any value as indicated hereinbefore. However, specific particularly preferred cyclopenta- g] quinazolines of the invention are:
N-{N-{4-[N-((6i?S)-2-methoxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl}-L-γ-glutamyl}-D-glutamic acid;
N-{N-{4-[N-((6RS)-2-hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[g-]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl}-L-γ-glutamyl}-D-glutamic acid; and
N- N-{4-[N-((6RS)-2-hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl} -L-γ-glutamyl} -N-methyl-L- -glutamic acid; or a pharmaceutically acceptable salt or ester thereof.
Although the compounds of the present invention can exist as a mixture of stereoisomers it is preferred that they are resolved into one optically active isomeric form. Such a requirement complicates the synthesis of the compounds and it is preferred therefore that they contain as few asymmetric carbon atoms as possible consistent with achieving the desired activity. As indicated previously, however, the cyclopenta[g]quinazolines of the present invention contain at least two asymmetric carbon atoms. Of these, that at the 6 position of the ring system preferably has the 6S orientation rather than the 6R orientation, whilst the alpha carbon atom of the group — CONHCH(CO H)— preferably has the L rather than the D configuration. The preferred compounds (I) described hereinbefore thus preferably have such a configuration at these two asymmetric carbon atoms or less preferably are a racemic mixture in which one or both of these asymmetric carbon atoms is unresolved.
The asymmetric carbon atom of a residue R3 of the form -A1-Ar2-A2-CON(R)CH(Y2)Y3 or -A5-CON(R)CH(Y4)Y5 may be of the L- or D-configuration but the amide bond will be stabilised in vivo when it is of the D-configuration as it will also be when R is other than hydrogen. When Y3 or Y5 is the residue of a naturally occurring amino acid, however, the amino acid intermediate for the synthesis of the cyclopenta[g]quinazoline will of course be more readily available when this asymmetric carbon atom is of the L-configuration.
A cyclopenta[g]quinazoline of the invention may be prepared by any process known to be applicable to the preparation of chemically-related compounds.
As stated above, cyclopenta[g] quinazolines of the present invention are believed to function as anti-cancer agents at least in part due to their ability to inhibit the enzyme thymidylate synthase. This anti-cancer activity may be assessed, for example, using one or more of the procedures set out below:
(a) An in vitro assay which determines the ability of a test compound to inhibit the enzyme thymidylate synthase. Thymidylate synthase may be obtained in partially purified form from L1210 mouse leukaemia cells and utilised in the assay using the procedures described by Jackman et al. (Cancer Res., 1986, 46, 2810) and Sikora et α/. (Biochem. Pharmacol, 1988, 37, 4047);
(b) An assay which determines the ability of a test compound to bind to the α-FR relative to that of folic acid, using mouse L1210-FBP cells (α-FR expression) in the procedure described by Westerhof et al. (Cancer Res., 1991, 51, 5507-5513);
(c) An assay which determines the ability of a test compound to inhibit the growth of human tumour cell lines expressing the α-FR (A431-FBP vulvular carcinoma transfected with the α-FR; KB nasopharengeal carcinoma); (d) An assay which determines the ability of a test compound to inhibit the growth of human tumour cell lines not expressing the α-FR (A431 neo-transfected);
(e) An assay confirming or demonstrating that compound-induced growth inhibition is largely attributable to α-FR mediated uptake into KB or A431-FBP cells. This involves the co-addition of an excess of folic acid (1 μM) to compete with the compounds for FR but not RFC binding.
Although the pharmacological properties of the cyclopenta[ ] quinazolines of the invention depend on their detailed structure, in general the cyclopenta[g]quin- azolines of the invention possess activity in one or more of the above tests (a) to (d) as indicated below:
Test (a) IC50 in the range, for example, 0.0001-1 μM;
Test (b) Inverse relative affinity in the range, for example, 0.05-5
(values greater than 1 implies binding is greater than that of folic acid and values less that 1 implies binding weaker than that of folic acid) ;
Test (c) IC50 in the range, for example, 0.001-10 μM;
Test (d) IC50 in the range, for example, 0.01-100 μM;
Test (e) IC50 at least 10-fold higher than that seen in test (b) for the same cell line. A cyclopenta[g]quinazoline of the present invention may itself be active or it may be a pro-drug which is converted in vivo to an active compound. A cyclopenta[g]quinazoline of the invention may be administered to a warm-blooded animal, including a human, in the form of a pharmaceutical composition which comprises the cyclopenta[g]quinazoline in association with a pharmaceutically- acceptable diluent or carrier.
The composition may be in a form suitable for oral use, for example a tablet, capsule, aqueous or oily solution, suspension or emulsion; a form suitable for topical use, for example a cream, ointment, gel or aqueous or oily solution or suspension; a form suitable for nasal use, for example a snuff, nasal spray or nasal drops; a form suitable for vaginal or rectal use, for example a suppository; a form suitable for administration by inhalation, for example as a finely divided powder such as a dry powder, a microcrystalline form or a liquid aerosol; a form suitable for sub-lingual or buccal use, for example a tablet or capsule; or a form suitable for parenteral use (including intravenous, subcutaneous, intramuscular, intravascular or infusion use), for example a sterile aqueous or oily solution, emulsion or suspension. In general the above compositions may be prepared in a conventional manner using conventional excipients.
The composition may contain, in addition to the cyclopenta[g]quinazoline of the invention, one or more other anti-cancer substances selected from, for example, other antimetabolites, DNA interacting agents, signal transduction inhibitors or other inhibitors of deregulated pathways in tumours.
The cyclopenta[g]quinazoline will normally be administered to a warmblooded animal at a dose within a range of 50-25000, particularly 50-5000, mg per square metre body area of the animal, i.e. approximately 1500, particularly 1-100, mg/kg. Where desired, however, dosages outside this range may be employed and, in particular, where the preferred mode of administration involving subcutaneous infusion is used then the does range may be increased to 1-1000 mg/kg. Preferably a daily dose in the range 10-250 mg/kg is employed, particularly 30-150 mg/kg. However, the daily dose will necessarily be varied depending upon the host treated, the particular route of administration and the severity of the illness being treated. Accordingly, the optimum dosage may be determined by the practitioner who is treating any particular patient.
Accordingly the present invention also includes a method for aiding regression and palliation of cancer in a patient, particularly a warm-blooded animal such as a human, in need of such treatment, which comprises administering to said patient an effective amount of a cyclopenta[g]quinazoline as defined hereinbefore. The invention also provides the use of such a cyclopenta[g]quinazoline in the manufacture of a novel medicament for use in the treatment of cancer. Cyclopenta[g]quinazolines of the present invention are of interest for a wide range of anti-tumour activities particularly the treatment of ovarian cancer.
In view of the activity shown by antimetabolites such as aminopterin and methotrexate, which is discussed hereinbefore, the cyclopenta[g]quinazolines of the present invention are also of interest for use in the treatment of other conditions, for example allergic conditions such as psoriasis and inflammatory diseases such as rheumatoid arthritis. In using a cyclopenta[g]quinazoline of the invention for such a purpose the compound will normally be administered at a dose within the range 5- 25000, particularly 5-500, mg per square metre body area of the animal, i.e. approximately 0.1-500, particularly 0.1-10, mg/kg. Where desired, however, dosages outside this range may be employed. In general, for the treatment of an allergic condition such as psoriasis, topical administration of a cyclopenta[g-]quinazoline of the invention is preferred. Thus, for example, for topical administration a daily dose in the range, for example, of 0.1 to 10 mg/kg may be used. Compositions containing the quinazolines may be formulated in unit dosage form, i.e. in the form of discrete portions each comprising a unit dose, or a multiple or sub-multiple of a unit dose, for example as a tablet or capsule. Such a unit dosage form may, for example, contain an amount of the cyclopenta[g]quinazoline in the range of 1-250 or 1-500 mg. The invention is illustrated by the following Examples.
Example 1: synthesis of CB300951 (2-CH2OMe derivative of CB300638)
Figure imgf000018_0001
CB300951
5-Methoxyacetamidoindan
To a solution of 5-aminoindan (4.66 g, 35.0 mmol) in anhydrous DMF (26 ml) was slowly added methoxyacetyl chloride (5.70 g, 52.50 mmol) followed by pyridine (8.5 ml, 105.0 mmol). The red solution was stirred at room temperature for 3.5 hours under argon, then it was partitioned between ethyl acetate (200 ml) and IN HCl (120 ml). The organic layer was washed with more IN HCl (120 ml), brine (100 ml), dried (Na2SO4) and concentrated in vacuo. The residue was triturated with diethyl ether; the white precipitate was collected by filtration, washed with diethyl ether to afford the title compound (5.93 g, 83%), m.p. 104-105 °C; Η-NMR (250 MHz, CDC13, TMS) 2.06 (m, 2H, 2-CH2), 2.87 (m, 4H, 1-CH2 and 3-CH2), 3.50 (s, 3H, OCH3), 4.00 (s, 2H, 2-CH2OMe), 7.22 (m (overlap with CHC13 peak), 2H, 6-H, 7-H), 7.52 (s, IH, 4-H), 8.18 (s, IH, CONH); MS (ESI, m/z): 432 [(2M+Na)+, 30%], 206[(M+H)+, 100%]; Found C, 70.10; H, 7.38; N, 6.81; Cι2H15NO2 requires C, 70.22; H, 7.37; N, 6.82%. 5-Methoxyacetamido-6-bromoindan
A mixture of 5-methoxyacetamidoindan (5.50 g, 0.027 mol) and glacial acetic acid (25 ml) was cooled in an ice-water bath (~10 °C). Bromine (1.5 ml, 0.029 mol) was then dropwise added over a 20 min period while the temperature was kept between 10-15 °C. The reaction mixture was then stirred for a longer 1 hour and then it was poured into an ice- water bath (100 ml) with the aid of water (70 ml). The precipitate was collected by filtration, washed with plenty of water (150 ml) and dried in vacuo over P2O5 to afford the title compound (6.98 g, 91%), m.p. 84-86 °C; 1H-NMR (250 MHz, CDC13, TMS) 2.09 (m, 2H, 2-CH2), 2.88 (m, 4H, 1-CH2 and 3-CH2), 3.55 (s, 3H, OCH3), 4.04 (s, 2H, 2-CH2OMe), 7.34, 8.22 (2 x s, 2H, 4-H, 7-H), 8.83 (s, IH, CONH);
MS (ESI, m/z): 284, 286 [(M+H)+, 98%, 100%; Br isotopic pattern]; Found C, 50.62; H, 4.93; N, 4.92; Br, 28.05; C12H14BrNO2 requires C, 50.72; H, 4.97; N, 4.93; Br, 28.12%.
5-Methoxyacetamido-6-bromoindan-l-one
To a solution of 5-methoxyacetamido-6-bromoindan (0.85 g, 3.0 mmol) in glacial acetic acid (7 ml) heated at 55 °C was dropwise added a solution of CrO3 (1.2 g, 12.0 mmol) in aqueous glacial acetic acid, (7 ml; v/v 1:1) over a 15 min period. The reaction mixture was then stirred at this temperature for 45 min. The reaction mixture was cooled in an ice-bath, then propan-2-ol (4 ml) was added and the mixture was stirred at this temperature for 10 min before being concentrated in vacuo. The black residue was broken up with a spatula with the aid of water and then partitioned between water (50 ml) and ethyl acetate (150 ml). The aqueous layer was extracted with more ethyl acetate (2 x 40 ml); the combined extracts were dried (Na2SO4), and concentrated in vacuo to give an off white residue. Purification by column chromatography on elution with 5% ethyl acetate in dichloromethane afforded in order of elution: a. 5-methoxyacetamido-6-bromoindan-l-one as a white solid which was further purified by trituration with ethyl acetate / hexanes (1:5, v/v): 0.50 g (55%), m.p. 162-163 °C; 1H-NMR (250 MHz, CDC13, TMS) 2.72 (m, 2H, 2-CH2), 3.11 (m,
2H, 3-CH2), 3.57 (s, 3H, OCH3), 4.09 (s, 2H, 2-CH2OMe), 7.95 (s, IH) and 8.65 (s,
IH) (2H, 4-H, 7-H), 9.27 (s, IH, CONH); MS (ESI, m/z) 298, 300 {(M+H)+, 100%, 97%» respectively, bromine isotopic pattern}; Found: C, 48.13; H, 3.99; N, 4.70; Br, 26.95; Cι2Hι2BrNO3 requires C, 48.34; H, 4.06; N, 4.70; Br, 26.80%); and b. 5-acetamido-6-bromoindan-3-one as a solid which was further purified by trituration with ethyl acetate / hexanes (1 :5, v/v): 0.026 g, (3 %), m.p. 149-151 °C 1H-NMR (250 MHz, CDC13, TMS) 2.71 (m, 2H, 2-CH2), 3.01 (m, 2H, 1-CH2), 3.56 (s, 3H, OCH3), 4.08 (s, 2H, 2-CH2OMe), 7.73 (s, IH) and 8.71 (s, IH) (2H, 4-H, 7-H), 8.97 (s, IH, CONH); MS (ESI, m/z) 298, 300 {(M+H)+, 100%, 98% respectively, bromine isotopic pattern}; Found: C, 47.95; H, 3.96; N, 4.59; Br, 26.63; C12H,2BrNO3 requires C, 48.34; H, 4.06; N, 4.70; Br, 26.80%).
tert-ButyI 4-[ΛH5-methoxyacetamido-64)romoindan-l-yl)amino]benzoate
Method A: To a flask containing 5-methoxyacetamido-6-bromoindan-l-one
(0.357 g, 1.2 mmol), 4-toluenesulfonic acid monohydrate (0.015 g), and tert-butyl
4-aminobenzoate (0.289 g, 1.5 mmol) was added 1,2-dimethoxyethane (dried by distillation over CaH2; 15 ml). An Aldrich azeotropic distillation apparatus containing molecular sieves (3A) was fitted to the reaction flask that was placed in an oil bath preheated to 115 °C. The reaction mixture was stirred at this temperature for 3.5 hours under argon; then allowed to cool to room temperature, and a solution of sodium cyanoborohydride in tetrahydrofuran (1M; 1.55 ml. 1.55 mmol) was added followed immediately by acetic acid (0.044 ml). The black reaction mixture was stirred at room temperature for 1 hour under argon; then it was partitioned between ethyl acetate (150 ml) and saturated aqueous sodium bicarbonate (100 ml). The aqueous layer was extracted with more ethyl acetate (100 ml); the organic extracts were combined, washed with brine (100 ml), dried (Na2SO4), and concentrated in vacuo to leave a reddish residue. Purification by column chromatography, on elution with 35%) ethyl acetate in petroleum ether (60-80 °C), afforded the desired product as a white solid: 0.175 g (31 %).
Method B: To a nearly clear solution of 5-methoxyacetamido-6-bromo- indan-1-one (0.300 g, 1.0 mmol) in anhydrous methanol (40 ml) was added tert-butyl 4-aminobenzoate (0.193 g, 1.0 mmol) followed by decaborane (0.044 g). The reaction mixture was stirred at room temperature for 24 hours before being concentrated in vacuo. Purification by column chromatography, on elution with 35% ethyl acetate in petroleum ether (60-80 °C), afforded a white solid that was further purified by reprecipitation from dichloromethane / hexane: 0.340 g, (72%) m.p. 152-153 °C; Η-NMR (250 MHz, CDC13, TMS) 1.57 (s, 9H, C(CH3)3), 1.93, 2.63 (2 x m, 2H, indanyl 2-H), 2.97 (m, 2H, indanyl 3-H), 3.55 (s, 3H, OCH3), 4.06 (s, 2H, CH2OMe), 5.04 (t, J = 6.50 Hz, IH, 1-H), 6.64 (d, J = 8.78 Hz, 2H, 3,5-H), , 7.51, 8.33 (2 x s, each IH, indanyl 4-H, 7-H), 7.85 (d, J = 8.75 Hz, 2,6-H), 8.93 (s, IH, CONH); MS (ESI, m/z) 499, 497 {(M+Na)+, bromine isotopic pattern}.
tert-Butyl 4-[A^(5-methoxyacetamido-6-cyanoindan-l-yI)amino]benzoate
To a solution of tert-butyl 4-[N-(5-methoxyacetamido-6-bromoindan-l-yl)- amino]benzoate (0.714 g, 1.50 mmol) in ΝMP (8 ml) [l-methyl-2-pyrrolidone] was added copper (I) cyanide (0.230 g, 2.55 mmol). The reaction mixture was placed in an oil-bath preheated to 140 °C and stirred at this temperature for 2 h. More copper (I) cyanide (0.100 g, 1.10 mmol) was then added and stirring was continued for a longer 3 hours. The reaction mixture was allowed to cool to room temperature, then poured into a mixture of aqueous ammonia (d = 0.88, 7 ml) and ice (~20 ml) and the resulting brown mixture was stirred at room temperature for ~5 min. The brown solid was collected by filtration washed with plenty of water, then suspended in dichloromethane (100 ml). The mixture was stirred at room temperature for 10 min, dried (Νa2SO4), and concentrated in vacuo. Purification by column chromatography, on elution with 35% ethyl acetate in hexane, afforded an off white solid that was reprecipitated from dichloromethane-ethyl acetate /hexane: 0.328 g, (52%) m.p. 163- 164 °C 1H-NMR (250 MHz, DMSO-d6, TMS) 1.50 (s, 9H, C(CH3)3), 1.85, 2.58 (2 x m, 2H, indanyl 2-H), 2.89 (m, 2H, indanyl 3-H), 3.41 (s, 3H, OCH3), 4.05 (s, 2H, CH2OMe), 5.06 (m, IH, indanyl 1-H), 6.73 (d, J = 8.82 Hz, 2H, 3,5-H), 6.82 (d, J = 8.37 Hz, IH, N10-H), 7.59, 7.57 (2 x s, each IH, indanyl 4-H, 7-H), 7.66 (d, J = 8.77 Hz, 2,6-H), 9.88 (s, IH, CONH); MS (ESI, m/z) 444 {(M+Na)+, 100%}; Found: C, 68.21; H, 6.47; N, 9.81; C24H27N3O4 requires C, 68.39; H, 6.46; N, 9.97%.
tørf-Butyl 4-{-V-[(6RS)-2-methoxymethyl-4-oxo-3,4,7,8-tetrahydro-6 -r- -cyclopenta [g] quinazoIin-6-yI] amino} benzoate
A mixture of tert-butyl 4-[N-(5-methoxyacetamido-6-cyanoindan-l-yl)- amino]benzoate (0.295 g, 0.70 mmol), ethanol (3.2 ml), and water (0.64 ml) was cooled in an ice-bath, then 30% aqueous H2O2 solution (0.60 ml) was added followed by granulated sodium hydroxide pellets (0.047 g, 1.19 mmol). The reaction mixture was stirred at ~0 °C for 10 min, then it was placed in an oil bath preheated to 55 °C and stirred at this temperature for 30 min. The reaction mixture was allowed to cool to room temperature, then the solvents were removed in vacuo and the residue was suspended in water (~15 ml). The pH of this mixture was adjusted to ~12 with IN NaOH (got a clear solution), then to ~4 with IN hydrochloric acid. The off white precipitate was collected by filtration, washed with water, dried in vacuo over P2O5: 0.262 g (89%), m.p. >122 °C (softens); 1H-NMR (250 MHz, DMSO-d6, TMS) 1.50 (s, 9H, C(CH3)3), 1.87, 2.56 (2 x m, each IH, 7-H), 3.00 (m, 2H, 8-H), 3.34 (s, 3H, OCH3), 4.30 (s, 2H, 2-CH2), 5.16 (m, IH, 6-H), 6.78 (d, J = 8.55 Hz, 2H, 3\5'-H), 6.89 (d, J = 8.10, N10-H), 7.52, 7.90 ( 2 x s, each IH, 5-H, 9-H), 7.67 (d, J = 8.45 Hz, 2',6'-H), 12.12 (s, IH, N3-H); MS (ESI, m/z) 444 {(M+Na)+, 40%}; Found: C, 67.19; H, 6.31; N, 9.69; C24H27N3O4 0.5H2O requires C, 66.96; H, 6.50; N, 9.76%.
Dicobalthexacarbonyl propargyl alcohol complex
This is a known compound (K.-D. Roth and U. Muller, Tetrahedron Letters 1993, 34, 2919) and in this study was prepared according to Nicholas' methodology (K. L. Salazar and K. M. Nicholas, Tetrahedron 2000, 56, 2211): To a round bottom flask charged with Co2(CO)6 (5.12 g, 15.0 mmol) under argon in a well ventilated hood was added anhydrous dichloromethane (170 ml) followed by a solution of propargyl alcohol (0.840 g, 15.0 mmol) in anhydrous dichloromethane (20 ml). The deep red reaction mixture was stirred at room temperature for 7 h under argon, then it was filtered through a thin layer of neutral alumina. The filtrate was concentrated in vacuo to give a red residue. Purification by column chromatography, on elution with 40%) diethyl ether in hexane, afforded the desired product as a red solid 4.10 g (80%); 1H-NMR (250 MHz, CDC13, TMS) 1.83 (t, J = 6.0 Hz, IH, OH), 4.80 (d, J = 6.0 Hz, 2H, CH2), 6.08 (s, IH, C-H).
(Propargyl)Co2(CO)6+BF4- This is a known compound (K.-D. Roth and U. Muller, Tetrahedron Letters
1993, 34, 2919) and in this study was prepared according to Nicholas' methodology (K. L. Salazar and K. M. Nicholas, Tetrahedron 2000, 56, 2211): To a round bottom flask charged with dicobalthexacarbonyl propargyl alcohol complex (1.60 g, 4.7 mmol) under argon was added (syringed via a septum) propionic acid (2.2 ml). The reaction mixture was cooled to -20 °C and then a solution of HBF4 in diethyl ether (54% w/w, 2.05 ml) was slowly syringed into the reaction mixture via a septum. The reaction mixture was stirred at -20 °C for 40 min, then cooled diethyl ether (50 ml) was added. Trituration afforded a red precipitate that was collected by filtration, washed with plenty of dry diethyl ether and dried in vacuo over P2O5: 1.71 g (90%). This was immediately used in the next reaction without any further purification.
tert-Butyl 4-{Λr-[(6RS)-2-methoxymethyl-4-oxo-3,4,7,8-tetrahydro-6H- -cyclopenta[g]quinazolin-6-yl]-iV-(prop-2-ynyl)amino}benzoate
To a round-bottomed flask containing the tetrafluoroborate salt (propargyl)Co2(CO)6 +BF4 " (0.271 g, 0.66 mmol) was added anhydrous dichloromethane (dried by distillation over P2O5; 22 ml). The nearly clear red dark solution was stirred at room temperature for few minutes under argon, then tert-butyl 4- {N-[(6RS)-2-methoxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta[ ]quinazolin- -6-yl] amino} benzoate (0.215 g, 0.51 mmol) was added in one portion. Stirring was continued at this temperature for 5 min then diisopropylethylamine ( 0.18 ml, 1.04 mmol) was added and the reaction mixture was stirred at room temperature for 45 min under argon. The reaction mixture was partitioned between ethyl acetate (150 ml) and brine (60 ml). The aqueous layer was extracted with more ethyl acetate (2 x 50 ml). The combined extracts were washed with 10% aqueous citric acid (50 ml), brine (50 ml), dried (Νa2SO4), and concentrated in vacuo. Purification by column chromatography, on elution with 40% ethyl acetate in dichloromethane, gave a red solid: 0.285 g (75%). To a solution of this complex (0.267 g, 0.36 mmol) in ethanol (60 ml) was added Fe(NO3)3.9Η2O (~ 8.0 g). The clear solution was stirred at room temperature for 10 min then a second portion of Fe(NO3)3.9H2O (-4.0 g) was added. The reaction mixture was stirred at room temperature for a longer 5 min then a final portion of Fe(NO3)3.9H2O (~5.0 g) was added; the nearly clear solution was turned into a dark red mixture. Stirring was continued at room temperature for an extra 25 min, then the reaction mixture was partitioned between ethyl acetate (150 ml) and dilute brine (700 ml). The aqueous layer was extracted with more ethyl acetate (2 x 70 ml), The combined organics were washed with brine (3 x 70 ml), dried (Na2SO4), and concentrated in vacuo. Purification by column chromatography, on elution with 2% methanol in dichloromethane, afforded a white solid which was reprecipitated from dichloromethane / hexane: 0.122 g (74%), m.p. 191-192 °C; 1H-NMR (250 MHz, DMSO-d6, TMS) 1.51 (s, 9H, C(CH3)3), 2.23, 2.53 (m-obscured by DMSO peak) (2 x m, each IH, 7-H), 2.95-3.20 (m, 3H, C≡CH, 8-H), 3.34 (s (obscured by the H2O peak), 3H, OCH3), 3.96 (ABq, J = 18.0 Hz, 2H, CH2C≡C), 4.31 (s, 2H, 2-CH2), 5.79 (t, J = 7.0 Hz, IH, 6-H), 7.02 (d, J = 9.01 Hz, 2H, 3',5'-H), 7.58, 7.80 ( 2 x s, each IH, 5-H, 9-H), 7.76 (d, J = 8.45 Hz, 2',6'-H), 12.17 (s, IH, N3-H); MS (ESI, m/z) 482 {(M+Na)+, 10%}; Found: C, 70.32; H, 6.31; N, 9.09; C27H29N3O4 requires C, 70.57; H, 6.36; N, 9.14%.
4-{N-[(6RS)-2-Methoxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta-
[g]qumazolin-6-yl]-N-(prop-2-ynyl)amino}benzoic acid
A solution of tert-butyl 4-{N-[(6RS)-2-methoxymethyl-4-oxo-3,4,7,8-tetra- hydro-6H-cyclopenta[g]quinazolin-6-yl]-N-(prop-2-ynyl)amino}benzoate (0.069 g, 0.15 mmol) in dichloromethane (1 ml) and trifluoroacetic acid (3 ml) was stirred at room temperature for 1 hour and 10 min, then the solvents were removed in vacuo. The residue was triturated with diethyl ether and the precipitate was collected by filtration, washed with diethyl ether and dried in vacuo over P2O5 to afford the title compound as the trifluoroacetate salt: 0.061 g, m.p. 225 °C (dec); 1H-ΝMR (250 MHz, DMSO-d6, TMS) 2.23, 2.53 (obscured by DMSO peak) (2 x m, each IH, 7-H), 2.90-3.20 (m, 3H, C≡CH, 8-H), 3.34 (s (obscured by the H2O peak), 3H, OCH3), 3.97 (ABq, J = 18.0 Hz, 2H, CH2C≡C), 4.32 (s, 2H, 2-CH2), 5.79 (t, J = 8.1 Hz, IH, 6-H), 7.03 (d, J = 9.02 Hz, 2H, 3',5'-H), 7.58, (s, IH, 9-H), 7.81 (m, 3H, 5-H, 2',6'-H), 12.17 (s, IH, N3-H); MS (ESI, m/z) 426 {(M+Na)+, 25%}, 404 {(M+H)+, 70%}.
Tri-tert-butyl N-{7V-{4-[/V-((6RS)-2-methoxymethyl-4-oxo-3,4,7,8-tetra- hydro-6H-cyclopenta[g]quinazoIin-6-yl)-N-(prop-2-ynyl)amino]benzoyI}- -L-γ-glutamyl}-D-glutamate
To a mixture of 4-{N-[(6RS)-2-methoxymethyl-4-oxo-3,4,7,8-tetrahydro- -6H-cyclopenta[g]quinazolin-6-yl]-N-(prop-2-ynyl)amino}benzoic acid trifluoro- acetate salt (0.056 g, -0.14 mmol), tri-tert-butyl L-γ-glutamyl-D-glutamate (0.090 g, 0.20 mmol) and anhydrous DMF (2.5 ml) was added diethyl cyanophosphonate (0.050 g, 0.31 mmol) followed by triethylamine (0.035 g, 0.35 mmol). The clear solution was stirred at room temperature for 2 hours, then it was partitioned between ethyl acetate (150 ml) and water (80 ml). The aqueous layer was extracted with ethyl acetate (2 x 70 ml). The combined organics were washed with 10% aqueous citric acid (2 x 40 ml), saturated sodium bicarbonate solution (100 ml) and brine (100 ml), dried (Na2SO4), and concentrated in vacuo. Purification by column chromatography, on elution with 1.5% methanol in ethyl acetate, afforded a white solid that was further purified by trituration with hexane with the aid of some dichloromethane: 0.072 g (64 %); m.p. >120 °C; 1H-NMR (250 MHz, DMSO-de, TMS) 1.38, 1.39, 1.41 (3 x s, 27H, 3 x C(CH3)3), 1.60-2.35 (m, 9H, 2 x β-CH2, 2 x γ-CH2, 7-H), 2.52 (m obscured by DMSO peak, IH, 7-H), 2.90-3.25 (m, 3H, C≡CH, 8-H), 3.97 (ABq, J = 17.0 Hz, 2H, CH2C≡C), 4.08, 4.12 (2 x m, 2H, 2 x α-CH), 4.32 (s, 2H, 2-CH2), 5.77 (t, J = 7.50 Hz, IH, 6-H), 7.02 (d, J = 8.85 Hz, 2H, 3',5-H), 7.58 (s, IH, 9-H), 7.80 (d, J = 9.0 z, 2H, 2',6'-H), 7.82 (s, IH, 5-H), 8.17 (d, J = 8.12 Hz, IH, CONH), 8.36 (d, J - 7.00 Hz, IH, CONH), 12.16 (s, IH, N3-H); MS (ESI, m/z) 852 {(M+Na)+, 20%}, 830 {(M+H)+, 100%}; Found C, 64.71; H, 7.21; N, 8.27. C45H59N5O10 requires C, 65.12; H, 7.17; N, 8.44%.
iV-{N-{4-[Λr-((6RS)-2-Methoxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cycIo- penta[g]quinazolin-6-yl)--V-(prop-2-ynyl)amino]benzoyl}-L-γ-gIutamyl}- -D-glutamic acid A solution of tri-tert-butyl N-{N-{4-[N-((6i?S)-2-methoxymethyl-4-oxo-
-3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]- benzoyl}-L-γ-glutamyl}-D-glutamate (0.056 g, 0.07 mmol) in trifluoroacetic acid (4.5 ml) was stirred at room temperature for 1 hour and 10 min with protection from the light. The solvent was then removed in vacuo and the residue was suspended in water (4 ml). The pΗ was adjusted to -10 with IN ΝaOΗ, then to ~4 with IN hydrochloric acid. The white precipitate was collected by filtration and dried in vacuo over P2O5: 0.020 g (45%), m.p. 150-155 °C (softens); Η-ΝMR (250 MHz, DMSO-dό, TMS) 1.60-2.30 (m, 9H, 2 x β-CH2, 2 x γ-CH2, 7-H), 2.52 (m obscured by DMSO peak, IH, 7-H), 2.90-3.25 (m, 3H, C≡CH, 8-H), 3.36 (s, 3H, OCH3), 3.97 (ABq, J = 17.0 Hz, 2H, CH2C≡C), 4.20, 4.34 (2 x m, 2H, 2 x α-CH), 4.32 (s, 2H, 2-CH2), 5.77 (t, J = 8.02 Hz, IH, 6-H), 7.02 (d, J = 8.85 Hz, 2H, 3',5-H), 7.58 (s, IH, 9-H), 7.81 (d, J = 9.0 z, 2H, 2',6'-H), 7.83 (s, IH, 5-H), 8.14 (d, J = 8.12 Hz, IH, COΝH), 8.33 (d, J = 7.78 Hz, IH, COΝH), 12.10 (s, IH, Ν3-H); MS (ESI, m/z) 662 {(M+H)+, 100%}. Example 2: synthesis of CB300945 (2-CH2OH derivative of CB300638)
Figure imgf000026_0001
CB300945
2-HydroxymethyI-3,4,7,8-tetrahydro-6H-cyclopenta|g]quinazoIin-4-one
A solution of caesium acetate (14.4 g, 75.2 mmol) in dry DMF (40 ml) was heated to 60 °C under argon for 30 min. The mixture was cooled to 40 °C and a suspension of 2-chloromethyl-3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-4-one (L. Skelton, V. Bavetsias, A. Jackman, WO 00/050417-A1; 2.2 g, 9.4 mmol) in dry DMF (60 ml) was added via a cannula. The mixture was heated to 80 °C under argon for 16 h. The mixture was cooled to room temperature and the solvent was removed in vacuo. The residue was suspended in water (50 ml) and MeOΗ (20 ml). The pΗ was adjusted to 12.5 with IM sodium hydroxide solution and the brown suspension was stirred for 2 h at room temperature. The insoluble brown solid was removed by filtration and the resulting solution was acidified to pΗ 5 with IM hydrochloric acid. The precipitate was collected by filtration, washed with acidified water and dried in vacuo over P2O5 to yield the product as a pale yellow solid (1.17g, 58%); m.p. 205- 210 °C; 1H NMR (DMSO- 6) δ 2.07 (quin, J = 7.4 Ηz, 2Η, 7-H), 2.98 (q, J = 6.95 Hz, 4H, 6-H and 8-H), 4.38 (s, 2H, 2-CH2), 7.46 (s, IH, 9-H), 7.92 (s, IH, 5-H); MS (FAB-m/z): Found 217 [(M+H)+, 100%]; HRMS: measured 217.0977; calculated for Cι2H,3N2O2 (M+H)+: 217.0977; Found C, 64.01; H, 5.23; N, 12.34. Ci2H,3N2θ2.1/2H2O requires C, 63.93; H, 5.77; N, 12.43%.
2-(2,2-Dimethylpropionyloxymethyl)-3,4,7,8-tetrahydro-6//-cycIopenta- [g]quinazolin-4-one
2-Hydroxymethyl-3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-4-one (1.0 g, 4.6 mmol), triethylamine (0.77 ml, 5.6 mmol), DMAP (50 mg, 0.4 mmol) and anhydrous CΗ C12 (50 ml) were mixed in a flask under argon. Pivalic anhydride (1.2 ml, 6.0 mmol) was added dropwise and the suspension stirred at room temperature under argon for 5 h. The solvent was removed in vacuo and the residue partitioned between EtOAc (100 ml) and saturated aqueous NaHCO3 (100 ml). The organic extract was washed with saturated aqueous NaHCO3 (70 ml), water (70 ml), brine (70 ml), dried (Na2SO4) and the solvent removed in vacuo. The residue was triturated with hexane (60 ml) and the product collected by filtration as a yellow solid (1.21 g, 87%); m.p. 185-190°C; 1H-NMR (DMSO-d6) δ 1.22 (s, 9H, CMe3), 2.07 (quin, J = 7.4 Hz, 2H, 7-H), 2.98 (q, J = 5.72 Hz, 4H, 6-H and 8-H), 4.94 (s, 2H, 2-CH2), 7.42 (s, IH, 9-H), 7.92 (s, IH, 5-H), 12.20 (br, IH, NH); MS (FAB, m/z): Found 301 [(M+H)+, 100%]; HRMS: measured 301.1539; calculated for C17H21N2O3 (M+H)+: 301.1552; Found C, 67.65; H, 6.54; N, 9.54. C17H20N2O3 requires C, 67.98; H, 6.71; N, 9.33%.
2-(2,2-Dimethylpropionyloxymethyl)-3,4,7,8-tetrahydro-6-r-T-cyclopenta- [g]quinazolin-4,6-dione and 2-(2,2-Dimethylpropionyloxymethyl)-3,4,7,8-tetra- hydro-6H-cyclopenta[g]quinazolin-4,8-dione
To a stirred solution of (Ph3SiO)2CrO2 (L. M. Baker and W. L. Carrick, J. Org. Chem. 1970, 35, 774) (10.6 mg, 0.017 mmol) in CH2C12 (5 ml) was added sequentially aqueous 70% tert-butyl hydroperoxide (0.18 ml, 1.3 mmol) and 2-(2,2-dimethylpropionyloxymethyl)-3,4,7,8-tetrahydro-6H-cyclopenta[^]quinazolin- -4-one (0.1 g, 0.33 mmol). The mixture was stirred at room temperature with protection from the light for 24 h. The solvents were removed in vacuo and the residue purified by column chromatography (20 g of silica gel) eluting with a gradient of 10-30% EtOAc in CΗC13 to yield 2-(2,2-dimethylpropionyloxymethyl)- -3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-4,6-dione as a white solid (47 mg, 45%); m.p. 185-190°C; 1H-NMR DMSO-d6) δ 1.23 (s, 9Η, CMe3), 2.72 (m, 2H, 7-H), 3.25 (m, 2H, 8-H), 5.00 (s, 2H, 2-CH2), 7.70 (s, IH, 9-H), 8.29 (s, IH, 5-H), 12.20 (br, IH, NH); MS (FAB, m/z): Found 315 [(M+H)+, 100%], 337 [(M+Na)+, 75%]; HRMS: measured 315.1360; calculated for Cι7Hι9N2O4 (M+H)+: 315.1345; Found C, 64.18; H, 5.72; N, 8.81. d7H18N2O4.0.2H2O requires C, 64.23; H, 5.79; N, 8.82%.
2-(2,2-Dimethylpropionyloxymethyl)-3,4,7,8-tetrahydro-6H-cyclopenta[g]- quinazolin-4,8-dione; Η-NMR (OMSO-d6) δ 1.23 (s, 9Η, CMe3), 2.76 (m, 2H, 7-H), 3.26 (m, 2H, 8-H), 4.98 (s, 2H, 2-CH2), 7.72 (s, IH, 9-H), 8.29 (s, IH, 5-H), 12.3 (br, IH, NH).
tert-Butyl 4-[-V-((6RS)-2-(2,2-dimethylpropionyloxymethyl)-4-oxo-3,4,7,8- -tetrahydro-6JΪ-cyclopenta[g]quinazolin-6-yl)amino]benzoate
A suspension of 2-(2,2-dimethylpropionyloxymethyl)-3,4,7,8-tetrahydro-6H- -cyclopenta[g]quinazolin-4,6-dione (0.47g, 1.50 mmol) in anhydrous methanol (33 ml) and anhydrous CΗ2CI2 (5 ml) was treated with tert-butyl 4-aminobenzoate (0.34 g, 1.78 mmol) followed by decaborane (0.07g, 0.58 mmol) and the mixture stirred at room temperature under argon for 18 h. The solvent was removed in vacuo and the residue purified by column chromatography (50g of silica gel) eluting with 30% ethyl acetate in CH2CI2 to yield the desired product as a white solid (0.43g, 58%); m.p. 231 °C; 1H-NMR (CDCI3) δ 1.26 (s, 9H, CMe3), 1.58 (s, 9H, CO2CMe3), 2.00 (m, IH, 7-H), 2.72 (m, IH, 7-H), 3.08 (m, 2H, 8-H), 5.10 (s, 2H, 2-CH2), 5.15 (m, IH, 6-H), 6.67 (d, J = 8.8 Hz, 2H, 3'-H, 5'-H), 7.58 (s, IH, 9-H), 7.87 (d, J = 8.8 Hz, 2H, 2'-H, 6'-H), 8.24 (s, IH, 5-H); MS (FAB, m/z): Found 491 [(M+H)+, 25%], 514 [(M+Na)+, 100%]; Found C, 68.37; H, 6.86; N, 8.35. C28H33N3O5 requires C, 68.41; H, 6.77; N, 8.55%.
terf-Butyl 4-[N-((6RS)-2-(2,2-dimethylpropionyloxymethyl)-4-oxo-3,4,7,8-
-tetrahydro-6H-cyclopenta[ ]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoate
A suspension of (propargyl)Co2(CO)6 +BF4 " (213 mg, 0.52 mmol) in anhydrous CH2C12 (25 ml) was treated with tert-butyl 4-[N-((6i?S)-2-(2,2-dimethyl- propionyloxymethyl)-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl)- amino]benzoate (200 mg, 0.41 mmol) and the red solution stirred at room temperature under argon for 15 minutes. Diisopropylethylamine (0.15 ml, 0.86 mmol) was added and the mixture stirred at room temperature under argon for 1 h. The mixture was partitioned between ethyl acetate (30 ml) and brine (30 ml). The organic extract was dried (Νa2SO4) and the solvent removed in vacuo. The residue was purified by column chromatography (20g of silica gel) eluting with a gradient of 0-10% ethyl acetate in CΗ2C12 to yield the complex as a red oil (191 mg, 58%); Η-NMR (CDCI3) δ 1.26 (s, 9H, CMe3), 1.59 (s, 9H, CO2CMe3), 2.31 (m, IH, 7-H), 2.62 (m, IH, 7-H), 3.13 (m, 2H, 8-H), 4.57 (AB system, J = 16.9 Hz, 2H, propargyl CH2), 5.09 (s, 2H, 2-CH2), 5.63 (t, J = 8.3, IH, 6-H), 5.98 (s, IH, propargyl CH), 6.91 (d, J = 8.9 Hz, 2H, 3'-H, 5'-H), 7.61 (s, IH, 9-H), 7.90 (d, J = 8.9 Hz, 2H, 2'-H, 6'-H), 8.14 (s, IH, 5-H), 10.25 (br s, IH).
A solution of this complex (186 mg, 0.23 mmol) in ethanol (30 ml) was treated with Fe(NO3)3.9H2O (1.1 g) and the solution stirred at room temperature for 2 h. The solution was partitioned between ethyl acetate (30 ml) and water (30 ml). The organic extract was washed with brine (30 ml), dried (Na2SO4) and the solvent removed in vacuo. The residue was purified by column chromatography (20g of silica gel) eluting with 10% ethyl acetate in CH2CI2 to yield the desired product as a white solid (94 mg, 78%); m.p. 134 °C; 1H-NMR (CDC13) δ 1.32 (s, 9H, CMe3), 1.61 (s, 9H, CO2CMe3), 2.23 (s, IH, propargyl CH), 2.38 (m, IH, 7-H), 2.62 (m, IH, 7-H), 3.07 (m, IH, 8-H), 3.25 (m, IH, 8-H), 3.94 (AB system, J = 18.6 Hz, 2H, propargyl CH2), 5.12 (s, 2H, 2-CH2), 5.68 (t, J = 8.2 Hz, IH, 6-H), 6.99 (d, J = 9.1 Hz, 2H, 3'-H, 5'-H), 7.63 (s, IH, 9-H), 7.95 (d, J = 9.0 Hz, 2H, 2'-H, 6'-H), 8.16 (s, IH, 5-H), 9.55 (br s, IH); MS (ESI, m/z) 552 {(M+Na)+, 100%}, 530 {(M+H)+, 20%}; Found C, 70.14; H, 6.80; N, 7.73. C31H35N3O5 requires C, 70.30; H, 6.66; N, 7.93%.
4-[N-((6RS)-2-(2,2-DimethylpropionyIoxymethyl)-4-oxo-3,4,7,8-tetra- hydro-6H-cyclopenta[^]quinazolin-6-yl)--V-(prop-2-ynyl)amino]benzoic cid A solution of tert-butyl 4-[N-((6RS)-2-(2,2-dimethylpropionyloxymethyl)-
-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]- benzoate (80 mg, 0.15 mmol) in trifluoroacetic acid (5 ml) was stirred at room temperature with protection from the light for 1.5h. The solvent was removed in vacuo and the residue triturated with 1 : 1 diethyl ether and hexane to yield the desired product as a white solid (81 mg, TFA salt); m.p. 133 °C; Η-ΝMR (DMSO-d6) δ 1.23 (s, 9Η, CO2CMe3), 2.22 (m, IH, 7-H), 2.50 (m, IH, 7-H), 3.03 (m, 2H, 8-H), 3.14 (s, IH, propargyl CH), 3.97 (AB system, J = 18.8 Hz, 2H, propargyl CH2), 4.95 (s, 2H, 2-CH2), 5.79 (t, J = 8.6 Hz, IH, 6-H), 7.03 (d, J = 9.0 Hz, 2H, 3'-H, 5'-H), 7.51 (s, IH, 9-H), 7.81 (d, J = 6.6 Hz, 2H, 2'-H, 6'-H), 7.83 (s, IH, 5-H).
Tri-tørt-butyl -V-{Λr-{4-[-V-((6RS)-2-(2,2-dimethylpropionyIoxymethyl)- -4-oxo-3,4,7,8-tetrahydro-6-fy-cyclopentahj]quinazolin-6-yl)-/V-(prop-2-ynyl)- amino]benzoyI}-L-γ-glutamyl}-D-glutamate A solution of 4-[N-((6i?S)-2-(2,2-dimethylpropionyloxymethyl)-4-oxo- -3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoic acid (80 mg, 0.15 mmol) in anhydrous dimethylformamide (7 ml) was treated with tή-tert butyl-L-γ-glutamyl-D-glutamate (150 mg, 0.33 mmol), diethyl cyano- phosphonate (0.06 ml, 0.40 mmol) and triethylamine (0.06 ml, 0.40 mmol). The solution was stirred at room temperature under argon with protection from the light for 2.5 h. The solution was partitioned between ethyl acetate (25 ml) and water (25 ml). The aqueous layer was extracted with ethyl acetate (2 x 20 ml). The combined organic extracts were washed with 10% aqueous citric acid (2 x 30 ml), saturated aqueous ΝaΗCO3 (30 ml), dilute brine (30 ml), dried (Na2SO4) and the solvent removed in vacuo. The residue was purified by column chromatography (30g of silica gel) eluting with 40%> ethyl acetate in CH2C12 to yield the desired product as a white solid (94 mg, 62%); m.p. 109 °C; 1H-NMR (CDC13) δ 1.29 (s, 9H, -COCMe3), 1.43 (s, 9H, COOCMe3), 1.47 (s, 9H, COOCMe3), 1.48 (s, 9H, COOCMe3), 1.60-2.10 (m, 5H, 2 x glu β-CH2, 7-CH), 2.21 (s, IH, propargyl CH), 2.22-2.50 (m, 4H, 2 x glu γ-CH2), 2.59 (m, IH, 7-H), 3.08 (m, IH, 8-H), 3.20 (m, IH, 8-H), 3.92 (AB system, J = 19.0 Hz, 2H, propargyl CH2), 4.48, 4.76 (2 x m, 2H, 2 x glu α-CH), 5.12 (s, 2H, 2-CH2), 5.64 (t, J = 8.1 Hz, IH, 6-H), 6.99 (d, J = 8.8 Hz, 2H, 3'-H, 5'-H), 7.07 (m, 2H, 2 x CONH), 7.64 (s, IH, 9-H), 7.80 (d, J = 8.8 Hz, 2H, 2'-H, 6'-H), 8.13 (s, IH, 5-H); MS (ESI, m/z) 922 {(M+Na)+, 100%}, 900 {(M+H)+, 40%}; Found C, 64.85; H, 7.23; N, 7.33. C49H65N5On.0.5H2O requires C, 64.76; H, 7.27; N, 7.71%.
N-{7V-{4-[7V-((6RS)-2-Hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[g]quinazoIin-6-yI)-N-(prop-2-ynyl)amino]benzoyI}-L-γ-glutamyl}- -D-glutamic acid
Tri-tert-butyl N- {N- {4-[N-((6RS)-2-(2,2-dimethylproρionyloxymethyl)-4-oxo- -3,4,7,8-tetrahydro-6H-cyclopenta[ ]quinazolin-6-yl)-N-(prop-2-ynyl)amino]- benzoyl}-L-γ-glutamyl}-D-glutamate (80 mg, 0.09 mmol) was dissolved in trifluoroacetic acid (5 ml) and stirred at room temperature with protection from the light for 1 h. The solvent was removed in vacuo and the residue dissolved in methanol (3 ml) and water (3 ml). The pΗ of the solution was adjusted to pΗ 12 with IM sodium hydroxide solution and stirred at room temperature for 6 h. The solution was acidified to pΗ 4 with IM hydrochloric acid and cooled to 0°C. The precipitate was collected by filtration and dried under vacuum over P2O5 to yield the desired product as a pale brown solid (27 mg, 47%); m.p. 172 °C; 1H-NMR (DMSO-d6) δ 1.60-2.10 (m, 5H, 2 x glu β-CH2, 7-CH), 2.15-2.40 (m, 5H, 2 x glu γ-CH2, 7-H), 2.99 (m, IH, 8-H), 3.12 (s, IH, propargyl CH), 3.16 (m, IH, 8-H), 3.98 (AB system, J = 19.9 Hz, 2H, propargyl CH2), 4.18, 4.30 (2 x m, 2H, 2 x glu α-CH), 4.36 (s, 2H, 2-CH2), 5.58 (br s, IH, -OH), 5.77 (t, J = 7.9 Hz, IH, 6-H), 7.01 (d, J = 8.9 Hz, 2H, 3'-H, 5'-H), 7.54 (s, IH, 9-H), 7.80 (d, J = 8.5 Hz, 2H, 2'-H, 6'-H), 7.82 (s, IH, 5-H), 8.15 (d, J = 7.5 Hz, IH), 8.35 (d, J = 7.2 Hz, IH) (2 x CONH); MS (ESI, m/z) 670 {(M+Na)+, 45%}, 648 {(M+H)+, 100%}; HRMS: measured 648.2313; calculated for C32H35N5Oι0 (M+H)+: 648.2306.
Example 3: synthesis of CB300960 (JV-methyl derivative of CB300945)
Figure imgf000031_0001
CB300960
4-{7V-[(6RS)-2-HydroxymethyI-4-oxo-3,4,7,8-tetrahydro-6H-cyclopentaIg] quinazolin-6-yI]-N-(prop-2-ynyl)amino}benzoic acid
Method A: A solution of tert-butyl 4-{N-[(6RS)-2-(2,2-dimethyl- propionyloxymethyl)-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta[g-]quinazolin-6-yl]-N- -(prop-2-ynyl)amino}benzoate (0.150 g, 0.28 mmol) in dichloromethane (2 ml) and trifluoroacetic acid (6 ml) was stirred at room temperature for 1 hour. The solvents were then removed in vacuo, and the residue was suspended in methanol (3 ml) and water (5 ml). The pΗ was adjusted to -10 with IN ΝaOΗ (1.1 ml), and the mixture was stirred at room temperature for 4 hours. The reaction mixture was diluted with water (5 ml) and the pΗ was adjusted to -5 with IN ΗC1. The solid was then collected by filtration, but 1H-ΝMR indicated no complete removal of the pivaloyl group. This solid was suspended into the filtrate and then IN NaOΗ (0.9 ml, 0.9 mmol) was added (pΗ - 12). The mixture was stirred at room temperature for 3.5 hours, then more IN NaOΗ (0.2 ml) was added, and the mixture was stirred at room temperature for a further 0.5 hours. The pH was then adjusted to -5.0 with IN HCl. The off-white precipitate was collected by filtration, washed with water, and dried in vacuo over P2O5: 0.086 g, (79%); 1H-NMR (250 MHz, DMSO-de, TMS) 2.22 (m, IH 7-CH), 2.90-3.30 (m, 3H, C≡CH, 8-H), 3.97 (ABq, J = 18.6 Hz, 2H, CH2C≡C), 4.37 (d, J = 6.1 Hz, 2H, 2-CH2), 5.56 (t, IH, CH2OH), 5.78 (t, J = 7.51 Ηz, 1Η, 6-Η), 7.03 (d, J = 8.9 Hz, 2H, 3', 5'-H), 7.55 (s, IH, 9-H), 7.82 (m, 3H, 2',6'-H, 5-H); MS (ESI, m/z) 779 {(2M+H)+, 100%}, 390 {(M+H)+, 60%}.
Method B: A solution of tert-butyl 4-{N-[(6i?S)-2-hydroxymethyl-4-oxo- -3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl]-N-(prop-2-ynyl)amino}- benzoate (0.050 g, 0.11 mmol) in dichloromethane (1 ml) and trifluoroacetic acid (2.4 ml) was stirred at room temperature for 1 hour. The solvents were then removed in vacuo, and the residue was triturated with diethyl ether. The off-white precipitate was collected by filtration, and washed with ether to obtain the desired product as the trifluoroacetate salt: 0.044 g.
Tri-terf-butyl N- Λr-{4-[N-((6RS)-2-hydroxymethyI-4-oxo-3,4,7,8-tetra- hydro-6H-cyclopenta[g]quinazolin-6-yl)-N-(prop-2-ynyl)aιnino]benzoyl}-L-γ-- -glutamyl}-iV-methyl-L-glutamate
To a mixture of 4-{N-[(67?S)-2-hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H- -cyclopenta[g]quinazolin-6-yl]-N-(prop-2-ynyl)amino}benzoic acid (0.075 g, -0.19 mmol), tri-tert-butyl L-γ-glutamyl-N-methyl-L-glutamate (N. Bavetsias et al., J. Med. Chem., 1997, 40, 1495-1510; 0.110 g, 0.24 mmol), and anhydrous DMF (2.0 ml) was added diethyl cyanophosphonate (0.036 g, 0.22 mmol) with the aid of anhydrous DMF (0.2 ml) followed by triethylamine (0.022 g, 0.22 mmol). The clear solution was stirred at room temperature for 1.5 hours, then it was partitioned between ethyl acetate (50 ml) and brine (40 ml). The aqueous layer was extracted with more ethyl acetate (2 x 50 ml). The combined organics were washed with 10% aqueous citric acid (40 ml), saturated sodium bicarbonate solution (40 ml), and brine (40 ml), dried (Νa2SO4), and concentrated in vacuo. Purification by column chromatography, on elution with a gradient of methanol in dichloromethane (0 to 6%), afforded an off-white solid that was further purified by trituration with hexane/dichloro- methane/diethyl ether: 0.062 g (40 %); mp 116-120 °C (softens); ' Η-NMR (250 MHz, DMSO-d6, TMS) 1.36, 1.37, 1.38, 1.41 (4 x s, 27H, 3 x C(CH3)3), 1.70-2.35 (m) and 2.50 (m obscured by DMSO peak) (10H, 2 x β-CH2, 2 x γ-CH2, 7-CH2), 2.63, 2.82 (2 x s, 3H, CONMe), 2.90-3.25 (m, 3H, C≡ R, 8-H), 3.97 (ABq, J = 17.6 Hz, 2H, CH2C≡C), 4.32 (m, IH, glu α-CH), 4.38 (d, J = 6.1 Hz, 2H, 2-CH2), 4.50, 4.82 (2 x dd, IH, Meglu α-CH), 5.56 (t, J = 6.9 Hz, IH, CH2OH), 5.78 (t, J = 7.10 Ηz, 1Η, 6-Η), 7.02 (d, J = 8.6 Hz, 2H, 3',5'-H), 7.55 (s, IH, 9-H), 7.78 (d, J = 8.9 Hz, 2H, 2',6'-H), 7.82 (s, IH, 5-H), 8.32 (m, IH, CONH), 11.81 (s, IH, N3-H); MS (ESI, m/z) 830{(M+H)+, 100%}.
Λ^-/7V-{4-[N-((6RS)-2-hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[^]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl}-L-y-glutamyI}-N- -methyl-L-glutamic acid
A solution of tri-tert-butyl N- N-{4-[N-((6i?S)-2-hydroxymethyl-4-oxo- -3,4,7,8-tetrahydro-6H-cyclopenta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]- benzoyl}-L-γ-glutamyl}-N-methyl-L-glutamate (0.060 g, 0.07 mmol) in trifluoroacetic acid (3.5 ml) was stirred at room temperature for 1 hour and 10 min with protection from the light. The solvent was then removed in vacuo and the residue was suspended in water (6 ml). The pΗ was adjusted to -10 with IN ΝaOΗ, then to -4 with IN hydrochloric acid. The white precipitate was collected by filtration, and dried in vacuo over P2O5: 0.035 g (77%), mp >165 °C (dec); Η-ΝMR (250 MHz, DMSO-d6, TMS) 1.80-2.35 (m) and 2.50 (m obscured by DMSO peak) (10H, 2 x β-CH2, 2 x γ-CH2, 7-CH2), 2.66, 2.83 (2 x s, 3H, COΝMe), 2.90-3.25 (m, 3H, C≡CH, 8-H), 3.97 (ABq, J = 18.4 Hz, 2H, CH2C≡C), 4.32 (m obscured, IH, glu α-CH), 4.38 (d, J = 5.6 Hz, 2H, 2-CH2), 4.55, 4.91 (2 x dd, J = 10.0, 4.5 Hz, IH, Meglu α-CH), 5.56 (poorly resolved t, IH, CH2OH), 5.77 (t, J = 8.06 Ηz, 1Η, 6-Η), 7.02 (d, J = 7.8 Ηz, 2Η, 3',5-H), 7.55 (s, IH, 9-H), 7.81 (d, J = 10.1 Hz, 3H, 2',6'-H, 5-H), 8.32 (m, IH, COΝH), 11.82 (s, IH, Ν3-H); MS (ESI, m/z) 662 {(M+H)+, 100%}; Found: C, 57.52; H, 5.52; N, 10.17; C33H35N5O,o 1.5 H2O requires: C, 57.55; H, 5.56; N, 10.17%.
Example 4: In vitro evaluation
CB300945, the (RS)-C2-CH2OH analogue of the known cyclopenta[ ]- quinazoline, CB300638 was synthesised as in Example 1. In addition, the C2-NH. was made as a comparative example. The activity of this new series of cyclopenta[ ]quinazolines with C2-CH3 (ΛS-CB300638), C2-CH2OH (RS- CB300945) and C2-NH2 (RS-CB300944) substitutions were compared with three other series of quinazoline derivatives in mouse L1210-FBP cells and human tumour cell lines co-expressing the RFC and α-FR. The human A431-FBP cell line was transfected with the α-FR and sensitivity compared with the A431 cell line. Human nasopharengeal KB cells constitutively overexpress this receptor (see Bagnoli et al. Oncogene, 19, 4754^1763, 2000).
In the quinazoline series, the C2-CH2OH analogues were found to have lower affinities for the α-FR of L1210-FBP cells (0.21 to 0.29) than the 2-CH3 (0.37 to 0.54) and particularly the 2-NH2 (1.3 to 1.7) counteφarts. In the cyclopenta[g]quin- azoline series the C2-CH3 analogue (CB300638) had a relative binding affinity of 0.57. However, unexpectedly the C2-CH2OH analogue (CB300945) had a higher affinity than predicted (0.71) and the C2-NH2 analogue (CB300944) had one lower than predicted (0.57). Thus in the cyclopenta[ ]quinazoline series C2-substitution did not markedly affect binding to the α-FR. A similar pattern of binding was noted for α-FR expressed by A431-FBP cells (data not shown).
A431-FBP and KB cells were highly sensitive to the C2-CH3 cyclopenta[g]- quinazoline with a L-glu-γ-D-glu ligand (S or RS-CB300638). However, very little activity of the C2-NH2 analogue (RS-CB300944) could be attributed to α-FR mediated uptake into either of these cell lines (Tables 13). On the other hand the C2- CH2OH analogue (RS-CB300945) was highly potent in A431-FBP and KB cells and more highly selective for these cells compared with A431 cells, than RS-CB300638. Tables 4 and 5 show the structures of the compounds tested.
Example 5: Formulation
The following illustrate representative pharmaceutical dosage forms containing a cyclopenta[g] quinazoline of formula (I), particularly in pharmaceutically acceptable salt form, for therapeutic or prophylactic use in humans:
(a) Tablet I mg/tablet
Cyclopenta[g] quinazoline salt 100
Lactose Ph.Eur. 182.75 Croscarmellose sodium 12.0
Maize starch paste (5% w/v paste) 2.25
Magnesium stearate 3.0
(b) Tablet II mg/tablet
Cyclopenta[g]quinazoline salt 50
Lactose Ph.Eur. 223.75
Croscarmellose sodium 6.0
Maize starch 15.0
Polyvinylpyrrolidone (5% w/v paste) 2.25
Magnesium stearate 3.0
(c) Tablet III mε/tablet
Cyclopenta[g] quinazoline salt 1.0
Lactose Ph.Eur. 93.25
Croscarmellose sodium 4.0
Maize starch paste (5% w/v paste) 0.75
Magnesium stearate 1.0
(d) Capsule mg/capsule
Cyclopenta[g]quinazoline salt 10.0
Lactose Ph.Eur. 488.5
Magnesium stearate 1.5
(e) Injection I (50 mg/ml)
Cyclopenta[g]quinazoline salt 5.0% w/v
IM Sodium hydroxide solution 15.0% v/v
0.1M Hydrochloric acid (to adjust pH to 7.6)
Polyethylene glycol 400 4.5% w/v
Water for injection to 100%
(0 Injection II (lO me/ml
Cyclopenta[g]quinazoline salt 1.0%) w/v
Sodium phosphate BP 3.6% w/v 0.1M Sodium hydroxide solution 15.0% v/v
Water for injection to 100%
(g) Injection III (1 mg/ml, buffered to pH 6) Cyclopenta[g]quinazoline salt 0.1% w/v
Sodium phosphate BP 2.26% w/v
Citric acid 0.38% w/v
Polyethylene glycol 400 3.5% w/v
Water for injection to 100%
The above formulations may be prepared by conventional procedures well known in the pharmaceutical art. The tablets (a) to (c) may be enteric coated by conventional means, for example with a coating of cellulose acetate phthalate.
Table 1 : Activity of 2-substituted quinazolines and eye lopenta[g] quinazolines in human A431 and A431-FBP cell lines grown in 20 nM folat
(RS LN)
Inhibition of cell growth, IC50, μM Inhibition of cell growth, IC50, μM
20nMLV 20nMLV
A431 A431+lμMFA A431-FBP A431-FBP+lμMFA (fold increased ICS0 in (fold increased sensitivity (fold increased lC$o in presence of folic acid) compared with A431) presence of folic acid)
CB30195 0.4 ±0.14 0.37 ±0.14 0.17 ±0.065 0.23 ± 0.87 0) 0)
CB300612 2.2 ±0.78 1.9 ±0.65 1.2 ±0.17 1.3 ±0.23 0) (2) 0)
CB300604 3.7 ±0.71 3.4 ±0.91 0.28 ± 0.057 1.2 ±0.59 0) (13) (4)
ZD9331 0.082 ± 0.042 0.067 ± 0.029 0.018 ±0.0097 0.034 ± 0.0087
(1) (4) (2)
CB300512 - - - -
CB300533 - - - -
CB300395 0.72 ±0.18 0.72 ±0.12 0.47 ± 0.20 0.50 ±0.17 ÷) 0) (2) 0)
CB300635 9.5 ±5.1 9.7 ±5.4 4.1 ±2.0 4.3 ±2.0 0) (2) ω
CB300616 5.9± 1.0 6.2 ±1.6 4.6 ± 0.40 4.7 ±1.1 0) (1) 0)
CB300638 (RS) 1.4 ±0.23 1.4 ±0.25 0.0065 ±0.0001 0.87 ± 0.29 0) (220) (130)
CB300944 (RS) 23,27 22,25 8.3 ±0.64 7.1 ±1.0 0) (3) 0)
CB300945 (RS) 9.813.4 9.3 ±3.5 0.0021 ±0.0011 6.5 ±0.86 0) (4700) (3100)
CB300960 (R,S) 4.5 4.3 0.028 5.1
0) (180)
Table 2: Activity of 2-substituted quinazolines and cyclopenta[g]quinazolines in human A431 and A431-FBP cell lines grown in 1 nM folate (
LN)
Inhibition of cell growth, IC50, μM Inhibition of cell growth, IC50, μM
InMLV InMLV
Figure imgf000038_0001
CB30195 0.25 ±0.13 0.21+0.078 0.10 ±0.032 0.22 ± 0.085 0) β) (2)
CB300612 1.8 ±0.91 1.5 ±0.51 1.8 ±0.68 2.9 ±0.55 0) (1) 0)
CB300604 1.6 ±0.36 2.0 ± 0.49 0.50 ±0.015 1.8 ±0.058 (I) β) (4)
ZD9331 0.061 ±0.014 0.056 ± 0.025 0.0088 ± 0.0052 0.027 ± 0.0076 0) (7) β)
CB300512 - - - -
CB300533 - - - -
CB300395 0.70 ± 0.072 0.74 ± 0.053 0.43 ± 0.36 0.54 ± 0.34 0) (2) 0)
CB300635 6.1 ±1.3 6.7 ±1.9 2.7 ±0.87 3.3 ±1.5 (I) (2) 0)
CB300616 4.9 ± 2.0 5.2 ±2.1 5.513.5 5.6 ±3.0
0) (1) 0)
CB300638 (RS) 1.2 ±0.21 0.98 ± 0.23 0.0072 ± 0.001 0.6110.012 0) (170) (85)
CB300944 (RS) 24 ± 3.6 24 ± 2.9 9.1 ±0.83 1212.1
0) β) 0)
CB300945 (RS) 7.2 ±3.6 7.3 ±3.3 0.0019 ±0.0006 5.711.3 (3800) (3100)
Table 3: Activity of 2-substituted quinazolines and cyclopenta[g]quinazolines in human KB cells grown in 1 and 20 nM folate (RS LV)
Inhibition of cell growth, IC50, μM Inhibition of cell growth, IC50, μM
InMLV 20nMLV
KB KB+lμMFA KB KB+lμMFA (fold increased IC50 in (fold increased IC$0 in presence of folic acid) presence of folic acid)
CB30195 0.005310.0006 0.1810.012 0.02710.002 0.1510.015 (34) (6)
CB300612 0.9710.20 2.210.35 1.710.20 2.010.25 (2) ω
CB300604 0.006110.00095 0.6910.08 0.03510.005 0.6510.04 (120) (20)
ZD9331 0.002110.0013 0.0064110.0035 0.003610.0021 0.01210.005 β) β)
CB300512 - - - -
CB300533 - - - -
CB300395 0.06310.081 0.2310.05 0.06510.0092 0.1710.032 (4) β)
CB300635 2.811.1 3.810.6 3.210.6 A A 10.25 0) 0)
CB300616 0.003710.0018 0.5810.05 0.00810.00072 0.5910.19 (160) (74)
CB300638 (RS) 0.007910.001 0.6110.012 0.005310.0025 0.7610.17
(76) (140)
CB300944 (RS) 1910 1911.5 1910.58 1911.2
(1) (1)
CB300945 (RS) 0.003410.0007 7.611.3 0.003410.0009 7.312.0
(2200) (2100)
Table 4: Structures of the compounds tested — Comparative compounds
Figure imgf000040_0001
ZD9331,R = Me CB300512,R = NH2 CB300533,R = CH2OH
Figure imgf000040_0002
CB300395, R = Me CB300635,R = NH2 CB300616,R = CH2OH
Figure imgf000040_0003
CB30195,R = Me CB300612,R = NH2 CB300604, R = CH2OH Table 5: Structures of the compounds tested — Compounds of the invention
Figure imgf000041_0001
CB300945
Figure imgf000041_0002
CB300638
Figure imgf000041_0003
CB300944
Figure imgf000041_0004

Claims

1. A cyclopenta[g] quinazoline of the formula (I):
Figure imgf000042_0001
(I) wherein:
A is a group OR0 or NR^1 wherein R° and Rl are each independently hydrogen, d- alkyl, C3-4 alkenyl, C3-4 alkynyl, C2^ hydroxyalkyl, C2-4 halogenoalkyl or d- cyanoalkyl, or R° and R1 together with the intermediate N form a five- or six-membered heterocyclic ring; p is an integer in the range 1 to 4;
R2 is hydrogen, -4 alkyl, C -4 alkenyl, C3-4 alkynyl, C2-4 hydroxyalkyl, C2-4 halogenoalkyl or C1-4 cyanoalkyl;
Ar1 is phenylene, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which may optionally bear one or two substituents selected from halogeno, hydroxy, amino, nitro, cyano, trifluoromethyl, C1-4 alkyl and d-4 alkoxy; and R3 is a group of the formula:
-A'-A^-A^Y1
in which A1 is a bond between the α-carbon atom of the group
-CO-NH-CH(CO2H)— and Ar2 or is a C1-2 alkylene group;
Ar2 is phenylene, tetrazolediyl, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which in the case of phenylene may optionally bear one or two substituents on the ring selected from halogeno, nitro, C1-4 alkyl and C1-4 alkoxy; A2 is a d_3 alkylene or C2_3 alkenylene group; and
Y1 is carboxyl, tetrazol-5-yl, N-(Cι-4 alkylsulfonyι)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d^» alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; or Y1 is a group of the formula:
-CON(R)CH(Y2)Y3
in which R is hydrogen, C1-4 alkyl, C3-4 alkenyl or C3-4 alkynyl;
Y2 is carboxy, tetrazol-5-yl, N-(Cι-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C1-4 alkyl and d_4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; and Y3 is the residue of a naturally occurring amino acid ΝH2CH(CO2H)Y3; or
Y is a group of the formula:
-A -CO2H
in which A4 is a C2_ό alkylene group;
R3 is a group of the formula:
-A5-CON(R)CH(Y4)Y5
in which A5 is a d_6 alkylene group and R is as defined above;
Y4 is carboxy, tetrazol-5-yl, N-(Cι-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C1- alkyl and - alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfinyl or tetrazol-5-ylsulfonyl; and Y5 is the residue of a naturally occurring amino acid ΝH CH(CO2H)Y5; or
Y5 is a group of the formula:
-A4-CO2H
in which A4 is as defined above; or
Y5 is a group of the formula:
-A6-Ar3-A7-Y6 in which A6 is a bond between the α-carbon atom of the group -A5-CON(R)CH(Y4)- and Ar3 or is a C1-2 alkylene group;
Ar3 is phenylene, tetrazolediyl, thiophenediyl, thiazolediyl, pyridinediyl or pyrimidinediyl which in the case of phenylene may optionally bear one or two substituents on the ring selected from halogeno, nitro, C1-4 alkyl and C1-4 alkoxy;
A7 is a Cι_3 alkylene or C2-3 alkenylene group; and
Y6 is carboxy, tetrazol-5-yl, N-(Cι-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, d-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfιnyl or tetrazol-5-ylsulfonyl; or
R is a group of the formula:
-A8-X-Ar4
in which A8 is a d-4 alkylene group;
X is sulflnyl, sulfonyl or methylene; and
Ar4 is l,2,3-triazol-4-yl, l,2,4-triazol-3-yl or, except when X is methylene, tetrazol-5-yl; the compound (I) optionally being in the form of a pharmaceutically acceptable salt or ester.
2. A cyclopenta[g]quinazoline as claimed in claim 1 wherein:
R° and R1 are each independently hydrogen or C1-4 alkyl;
R2 is ethyl, propyl, prop-2-enyl, prop-2-ynyl, 2-hydroxyethyl, 2-fluoroethyl, 2-bromoethyl or 2-cyanoethyl;
Ar1 is 1,4-phenylene which may optionally bear one or two substituents selected from the group consisting of chloro, fluoro, thiophene-2,5-diyl, thiazole-2,5- -diyl or pyridine-2,5-diyl;
R3 is a group of the formula:
-A5-COΝ(R)CH(Y4)Y5
in which A is a Cι_ό alkylene group and R is as defined in claim 1 ; Y4 is carboxy, tetrazol-5-yl, N-(d-4 alkylsulfonyl)carbamoyl, N-(phenyl- sulfonyl)carbamoyl which may optionally bear one or two substituents on the phenyl ring selected from the group consisting of halogeno, nitro, C1-4 alkyl and C1-4 alkoxy, tetrazol-5-ylthio, tetrazol-5-ylsulfιnyl or tetrazol-5-ylsulfonyl; and Y5 is the residue of a naturally occurring amino acid ΝH CH(CO2H)Y5.
3. A cyclopenta[g]quinazoline as claimed in claim 2 wherein: A is a group OR0 in which R° is hydrogen or methyl;
R2 is ethyl or prop-2-ynyl; and Ar1 is 1,4-phenylene or 1,4-phenylene having a 2-fluoro.
4. A cyclopenta[g]quinazoline as claimed in claim 3 in which R3 is the residue of an acid NH2.CH(COOH)R3 which comprises L-Glu-γ-D-Glu, i.e. N-L-γ-glutamyl-D-glutamic acid.
A cyclopenta[g]quinazoline as claimed in any preceding claim wherein p is 1.
6. A cyclopenta[g]quinazoline as claimed in claim 1 comprising:
N- {N- {4-[N-((6RS)-2-methoxymethyl-4-oxo-3 ,4,7,8-tetrahydro-6H-cyclo- penta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl} -L-γ-glutamyl} -D-glutamic acid;
N-{N-{4-[N-((6RS)-2-hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl}-L-γ-glutamyl}-D-glutamic acid; or N- N-{4-[N-((6RS)-2-hydroxymethyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclo- penta[g]quinazolin-6-yl)-N-(prop-2-ynyl)amino]benzoyl}-L-γ-glutamyl}-N-methyl-L-
-glutamic acid; or a pharmaceutically acceptable salt or ester thereof.
7. A cyclopenta[g]quinazoline according to any of the preceding claims for use in therapy.
8. A pharmaceutical composition comprising a cyclopenta[g] quinazoline according to any of Claims 1 to 6 together with a pharmaceutically acceptable diluent or carrier.
9. The use of a cyclopenta[g]quinazoline according to any of claims 1 to 6 for the manufacture of a medicament for use in the treatment of cancer.
10. The use according to claim 9 wherein the cancer comprises a carcinoma of ovarian origin.
11. A method for aiding regression and palliation of cancer in a patient in need of such treatment which comprises administering to said patient an effective amount of a cyclopenta[g] quinazoline according to any of Claims 1 to 6.
PCT/GB2002/003979 2001-08-31 2002-08-30 Anti-cancer cyclopenta[g]quinazoline compounds Ceased WO2003020748A1 (en)

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BRPI0212215A BRPI0212215B8 (en) 2001-08-31 2002-08-30 cyclopenta[g]quinazoline anticancer compounds, use thereof for the treatment of cancer and pharmaceutical composition comprising said compound
AT02755281T ATE483722T1 (en) 2001-08-31 2002-08-30 ANTI-CANCER CYCLOPENTAAG CHINAZOLINE DERIVATIVES
DK02755281.9T DK1421105T3 (en) 2001-08-31 2002-08-30 Anti-cancer cyclopenta [g] quinazoline compounds
US10/487,871 US7297701B2 (en) 2001-08-31 2002-08-30 Anti-cancer cyclopenta [G]quinazoline compounds
ZA2004/01587A ZA200401587B (en) 2001-08-31 2004-02-26 Anti-cancer cyclopenta[g]quinazoline compounds
US11/710,985 US7863284B2 (en) 2001-08-31 2007-02-27 Anti-cancer cyclopenta[G]quinazoline compounds
US11/727,242 US7705006B2 (en) 2001-08-31 2007-03-26 Anti-cancer cyclopenta[g]quinazoline compounds
US12/588,910 US8486955B2 (en) 2001-08-31 2009-11-02 Anti-cancer cyclopenta [g] quinazoline compounds
US12/659,286 US8063056B2 (en) 2001-08-31 2010-03-03 Anti-cancer cyclopenta [g]quinazonline compounds
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