WO2003027682A3 - Controlling isotope effects during fractionation of analytes - Google Patents

Controlling isotope effects during fractionation of analytes Download PDF

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Publication number
WO2003027682A3
WO2003027682A3 PCT/US2002/030742 US0230742W WO03027682A3 WO 2003027682 A3 WO2003027682 A3 WO 2003027682A3 US 0230742 W US0230742 W US 0230742W WO 03027682 A3 WO03027682 A3 WO 03027682A3
Authority
WO
WIPO (PCT)
Prior art keywords
analytes
controlling
isotope
effects during
during fractionation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/030742
Other languages
French (fr)
Other versions
WO2003027682A2 (en
Inventor
Fred E Regnier
Roujian Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Purdue Research Foundation
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Priority to AU2002341862A priority Critical patent/AU2002341862C1/en
Priority to CA2461587A priority patent/CA2461587C/en
Priority to EP02776017A priority patent/EP1432992A2/en
Publication of WO2003027682A2 publication Critical patent/WO2003027682A2/en
Publication of WO2003027682A3 publication Critical patent/WO2003027682A3/en
Anticipated expiration legal-status Critical
Priority to AU2008221639A priority patent/AU2008221639A1/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/534—Production of labelled immunochemicals with radioactive label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

Compositions and methods for controlling or eliminating isotope effects during fractionation of chemically equivalent but isotopically distinct compounds. Isotope coding agents contain heavy isotopes other than deuterium. The invention facilitates intelligent data acquisition. After sample fractionation, isotope abundance ratios are calculated using mass spectrometry, and analytes of interest are identified in real time.
PCT/US2002/030742 2001-09-27 2002-09-27 Controlling isotope effects during fractionation of analytes Ceased WO2003027682A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002341862A AU2002341862C1 (en) 2001-09-27 2002-09-27 Controlling isotope effects during fractionation of analytes
CA2461587A CA2461587C (en) 2001-09-27 2002-09-27 Materials and methods for controlling isotope effects during fractionation of analytes
EP02776017A EP1432992A2 (en) 2001-09-27 2002-09-27 Controlling isotope effects during fractionation of analytes
AU2008221639A AU2008221639A1 (en) 2001-09-27 2008-09-24 Controlling isotope effects during fractionation of analytes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32533501P 2001-09-27 2001-09-27
US60/325,335 2001-09-27

Publications (2)

Publication Number Publication Date
WO2003027682A2 WO2003027682A2 (en) 2003-04-03
WO2003027682A3 true WO2003027682A3 (en) 2004-02-26

Family

ID=23267455

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/030742 Ceased WO2003027682A2 (en) 2001-09-27 2002-09-27 Controlling isotope effects during fractionation of analytes

Country Status (5)

Country Link
US (2) US7449170B2 (en)
EP (1) EP1432992A2 (en)
AU (2) AU2002341862C1 (en)
CA (1) CA2461587C (en)
WO (1) WO2003027682A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449170B2 (en) 2001-09-27 2008-11-11 Purdue Research Foundation Materials and methods for controlling isotope effects during fractionation of analytes
US8097425B2 (en) 2006-03-10 2012-01-17 Tethys Bioscience, Inc. Multiplex protein fractionation
US9134318B2 (en) 2009-12-11 2015-09-15 Purdue Research Foundation Detection of oxidized polypeptides

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE344456T1 (en) * 2000-05-05 2006-11-15 Purdue Research Foundation AFFINITY-SELECTED SIGNATURE EPPTIDES FOR IDENTIFYING AND QUANTIFYING PROTEINS
US7632686B2 (en) * 2002-10-03 2009-12-15 Anderson Forschung Group High sensitivity quantitation of peptides by mass spectrometry
US7332613B2 (en) * 2003-06-06 2008-02-19 President And Fellows Of Harvard College Capture and release based isotope tagged peptides and methods for using the same
GB0314209D0 (en) * 2003-06-19 2003-07-23 Amersham Biosciences Ab Novel MS reagents
JP2007502837A (en) * 2003-08-18 2007-02-15 テシス バイオサイエンス， インコーポレイテッド Methods for reducing sample complexity using small epitope antibodies
WO2005024381A2 (en) * 2003-09-05 2005-03-17 Griffin Analytical Technologies, Inc. Analysis methods, analysis device waveform generation methods, analysis devices, and articles of manufacture
CA2553223A1 (en) * 2004-01-15 2005-08-04 Cedars-Sinai Medical Center System and method for expression proteomics based on isotope ratio modification
SE0401293D0 (en) * 2004-05-17 2004-05-17 Amersham Biosciences Ab Novel MS Reagents
GB2430794B (en) * 2004-05-20 2009-10-21 Waters Investments Ltd Method and apparatus for identifying proteins in mixtures
ATE427499T1 (en) * 2004-05-21 2009-04-15 Inst Systems Biology COMPOSITIONS AND METHODS FOR QUANTIFYING SERUM GLYCOPROTEINS
DE112005001385T5 (en) 2004-06-15 2007-05-10 Griffin analytical Technologies Inc., West Lafayette Analytical instruments, assemblies and procedures
WO2006012154A1 (en) * 2004-06-28 2006-02-02 Janssen Pharmaceutica, N.V. A method for detecting reactive metabolites using stable-isotope trapping and mass spectroscopy
CA2838007A1 (en) 2004-11-15 2006-05-26 University Of North Dakota A method for single oxygen atom incorporation into digested peptides using peptidases
US9097723B2 (en) * 2005-04-01 2015-08-04 Caliper Life Sciences, Inc. Method and apparatus for performing peptide digestion on a microfluidic device
US8680461B2 (en) 2005-04-25 2014-03-25 Griffin Analytical Technologies, L.L.C. Analytical instrumentation, apparatuses, and methods
US8580534B2 (en) * 2006-06-30 2013-11-12 The University Of North Dakota Method for incorporation of two oxygen atoms into digested peptides using peptidases
CN101101283A (en) * 2006-07-05 2008-01-09 中国科学院大连化学物理研究所 Method for separating and enriching phosphorylated peptide
US7992424B1 (en) 2006-09-14 2011-08-09 Griffin Analytical Technologies, L.L.C. Analytical instrumentation and sample analysis methods
EP2078033B1 (en) 2006-09-29 2011-03-09 Janssen Pharmaceutica NV Isotopically labeled trapping agent and method for identifying reactive metabolites
US7820964B2 (en) * 2007-08-06 2010-10-26 Metabolic Analyses, Inc Method for generation and use of stable isotope patterns in mass spectral data
WO2009134439A2 (en) * 2008-05-02 2009-11-05 Purdue Research Foundation Group specific internal standard technology (gsist) for simultaneous identification and quantification of small molecules
DE102008041813B4 (en) * 2008-09-04 2013-06-20 Carl Zeiss Microscopy Gmbh Method for the depth analysis of an organic sample
US8969795B2 (en) * 2008-10-06 2015-03-03 Shimadzu Corporation Curtain gas filter for mass- and mobility-analyzers that excludes ion-source gases and ions of high mobility
WO2010109022A1 (en) 2009-03-27 2010-09-30 Universitetet I Oslo Quantitative proteomics method
US8892184B2 (en) 2010-10-18 2014-11-18 Siemens Medical Solutions Usa, Inc. Systems and methods for reducing interference in a dual modality imaging system
EP2820425A1 (en) * 2012-02-02 2015-01-07 Perfinity Biosciences, Inc. Selector based recognition and quantification system and method for multiple analytes in a single analysis
US9048072B2 (en) * 2012-03-12 2015-06-02 Micromass Uk Limited Method of mass spectrometry and a mass spectrometer
US9786478B2 (en) 2014-12-05 2017-10-10 Purdue Research Foundation Zero voltage mass spectrometry probes and systems
CA2969251C (en) * 2014-12-08 2023-08-15 Arash Zarrine-Afsar System and method for enhanced mass spectrometry imaging
EP3254297B1 (en) 2015-02-06 2024-04-03 Purdue Research Foundation Probes, systems, and cartridges
US11266383B2 (en) 2015-09-22 2022-03-08 University Health Network System and method for optimized mass spectrometry analysis
JP7109374B2 (en) * 2016-04-14 2022-07-29 エフ．ホフマン－ラ ロシュ アーゲー A method for determining the concentration of a target analyte in a sample of bodily fluid
CN109932444B (en) * 2019-03-19 2022-03-11 北京泰德制药股份有限公司 Evaluation method for posttranslational modification of multiple charge isomers of glycoprotein
JP7651476B2 (en) * 2019-05-07 2025-03-26 ブライトスペック，インコーポレイテッド Highly selective chromatography-molecular rotational resonance spectroscopy system and method
EP4319787A4 (en) * 2021-04-08 2025-03-12 IQ Proteomics LLC Compounds comprising an isobaric region, an enrichment handle, and a thiol-reactive group, and methods of using the same
CN115266981B (en) * 2022-07-28 2023-06-13 四川阿格瑞新材料有限公司 Method for detecting deuteration rate of substance by GC-MS
WO2025250525A1 (en) * 2024-05-31 2025-12-04 Thermo Finnigan Llc Systems and methods for performing multiplexed mass spectrometry for quantitation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011208A1 (en) * 1998-08-25 2000-03-02 University Of Washington Rapid quantitative analysis of proteins or protein function in complex mixtures
WO2002046770A2 (en) * 2000-10-23 2002-06-13 Genetics Institute, Llc Isotope-coded ionization-enhancing reagents (icier) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340966C (en) 1989-05-19 2000-04-18 Thomas R. Covey Method of protein analysis
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5393669A (en) 1993-02-05 1995-02-28 Martek Biosciences Corp. Compositions and methods for protein structural determinations
ES2205437T3 (en) 1997-01-23 2004-05-01 XZILLION GMBH & CO.KG PROCEDURE THAT ALLOWS CHARACTERIZING POLYPEPTIDES.
EP1105731B1 (en) 1998-08-12 2006-10-18 Zycos Inc. Profiling and cataloging expressed protein tags
US6629040B1 (en) 1999-03-19 2003-09-30 University Of Washington Isotope distribution encoded tags for protein identification
US6391649B1 (en) 1999-05-04 2002-05-21 The Rockefeller University Method for the comparative quantitative analysis of proteins and other biological material by isotopic labeling and mass spectroscopy
ATE344456T1 (en) * 2000-05-05 2006-11-15 Purdue Research Foundation AFFINITY-SELECTED SIGNATURE EPPTIDES FOR IDENTIFYING AND QUANTIFYING PROTEINS
AU6689401A (en) 2000-06-12 2001-12-24 Univ Washington Selective labeling and isolation of phosphopeptides and applications to proteomeanalysis
US20020119490A1 (en) 2000-12-26 2002-08-29 Aebersold Ruedi H. Methods for rapid and quantitative proteome analysis
US7045296B2 (en) 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples
US7183116B2 (en) 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
EP1432992A2 (en) 2001-09-27 2004-06-30 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
WO2003102018A2 (en) 2002-06-03 2003-12-11 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US20070269895A1 (en) 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
US7655433B2 (en) 2002-06-04 2010-02-02 The Institute For Systems Biology Methods for high-throughput and quantitative proteome analysis
US7473535B2 (en) 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
WO2005007677A2 (en) 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
ATE427499T1 (en) 2004-05-21 2009-04-15 Inst Systems Biology COMPOSITIONS AND METHODS FOR QUANTIFYING SERUM GLYCOPROTEINS
US20070099251A1 (en) 2005-10-17 2007-05-03 Institute For Systems Biology Tissue-and serum-derived glycoproteins and methods of their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011208A1 (en) * 1998-08-25 2000-03-02 University Of Washington Rapid quantitative analysis of proteins or protein function in complex mixtures
WO2002046770A2 (en) * 2000-10-23 2002-06-13 Genetics Institute, Llc Isotope-coded ionization-enhancing reagents (icier) for high-throughput protein identification and quantitation using matrix-assisted laser desorption ionization mass spectrometry

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ZHANG R ET AL: "Fractionation of isotopically labeled peptides in quantitative proteomics.", ANALYTICAL CHEMISTRY. UNITED STATES 1 NOV 2001, vol. 73, no. 21, 1 November 2001 (2001-11-01), pages 5142 - 5149, XP002260710, ISSN: 0003-2700 *
ZHANG ROUJIAN ET AL: "Minimizing resolution of isotopically coded peptides in comparative proteomics.", JOURNAL OF PROTEOME RESEARCH. UNITED STATES 2002 MAR-APR, vol. 1, no. 2, March 2002 (2002-03-01), pages 139 - 147, XP002260715, ISSN: 1535-3893 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449170B2 (en) 2001-09-27 2008-11-11 Purdue Research Foundation Materials and methods for controlling isotope effects during fractionation of analytes
US7901943B2 (en) 2001-09-27 2011-03-08 Purdue Research Foundation Materials and methods for controlling isotope effects during fractionation of analytes
US8097425B2 (en) 2006-03-10 2012-01-17 Tethys Bioscience, Inc. Multiplex protein fractionation
US9134318B2 (en) 2009-12-11 2015-09-15 Purdue Research Foundation Detection of oxidized polypeptides

Also Published As

Publication number Publication date
US20030186326A1 (en) 2003-10-02
WO2003027682A2 (en) 2003-04-03
AU2002341862C1 (en) 2009-01-29
US20090148952A1 (en) 2009-06-11
AU2008221639A1 (en) 2008-10-30
US7449170B2 (en) 2008-11-11
EP1432992A2 (en) 2004-06-30
AU2002341862B8 (en) 2008-09-18
CA2461587C (en) 2011-04-26
US7901943B2 (en) 2011-03-08
AU2002341862B2 (en) 2008-08-07
CA2461587A1 (en) 2003-04-03

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