WO2003052142A3 - Analysis and detection of multiple target sequences using circular probes - Google Patents

Analysis and detection of multiple target sequences using circular probes Download PDF

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Publication number
WO2003052142A3
WO2003052142A3 PCT/NL2002/000834 NL0200834W WO03052142A3 WO 2003052142 A3 WO2003052142 A3 WO 2003052142A3 NL 0200834 W NL0200834 W NL 0200834W WO 03052142 A3 WO03052142 A3 WO 03052142A3
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WO
WIPO (PCT)
Prior art keywords
detection
target sequences
analysis
multiple target
multiplicity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2002/000834
Other languages
French (fr)
Other versions
WO2003052142A2 (en
Inventor
Eijk Michael Josephus Ther Van
Rene Cornelis Josephus Hogers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Keygene NV
Original Assignee
Keygene NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene NV filed Critical Keygene NV
Priority to EP02795451A priority Critical patent/EP1453978B9/en
Priority to CA002470356A priority patent/CA2470356A1/en
Priority to JP2003553009A priority patent/JP4226476B2/en
Priority to DE60219199T priority patent/DE60219199T2/en
Priority to US10/498,524 priority patent/US7498131B2/en
Priority to AU2002360223A priority patent/AU2002360223B2/en
Publication of WO2003052142A2 publication Critical patent/WO2003052142A2/en
Publication of WO2003052142A3 publication Critical patent/WO2003052142A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Radar Systems Or Details Thereof (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)

Abstract

Method for the high throughput separation and detection of a multiplicity of target sequences in a multiplicity of samples comprising subjecting each sample to a ligation-dependent amplification assay followed by a multiple injection step comprising the consecutive and/or simultaneous injection of a multiplicity of samples, for instance in a multichannel electrophoretic device.
PCT/NL2002/000834 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using circular probes Ceased WO2003052142A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP02795451A EP1453978B9 (en) 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using circular probes
CA002470356A CA2470356A1 (en) 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using circular probes
JP2003553009A JP4226476B2 (en) 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using a circularizable probe
DE60219199T DE60219199T2 (en) 2001-12-14 2002-12-16 ANALYSIS AND DETECTION OF MULTIPLE TARGET SEQUENCES USING CIRCULAR SAMPLES
US10/498,524 US7498131B2 (en) 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using circular probes
AU2002360223A AU2002360223B2 (en) 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using circular probes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01204912A EP1319718A1 (en) 2001-12-14 2001-12-14 High throughput analysis and detection of multiple target sequences
EP01204912.8 2001-12-14

Publications (2)

Publication Number Publication Date
WO2003052142A2 WO2003052142A2 (en) 2003-06-26
WO2003052142A3 true WO2003052142A3 (en) 2003-10-30

Family

ID=8181448

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/NL2002/000832 Ceased WO2003052140A2 (en) 2001-12-14 2002-12-16 High throughput analysis and detection of multiple target sequences
PCT/NL2002/000834 Ceased WO2003052142A2 (en) 2001-12-14 2002-12-16 Analysis and detection of multiple target sequences using circular probes
PCT/NL2002/000833 Ceased WO2003052141A2 (en) 2001-12-14 2002-12-16 High troughput analysis and detection of multiple target sequences using circular probes

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/NL2002/000832 Ceased WO2003052140A2 (en) 2001-12-14 2002-12-16 High throughput analysis and detection of multiple target sequences

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/NL2002/000833 Ceased WO2003052141A2 (en) 2001-12-14 2002-12-16 High troughput analysis and detection of multiple target sequences using circular probes

Country Status (9)

Country Link
US (1) US7498131B2 (en)
EP (2) EP1319718A1 (en)
JP (1) JP4226476B2 (en)
AT (1) ATE358186T1 (en)
AU (3) AU2002360223B2 (en)
CA (1) CA2470356A1 (en)
DE (1) DE60219199T2 (en)
ES (1) ES2284967T3 (en)
WO (3) WO2003052140A2 (en)

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AU2003220254B2 (en) * 2002-03-13 2008-09-18 Syngenta Participations, Ag. Nucleic acid detection method
EP1641936B1 (en) 2003-06-17 2010-08-04 Human Genetic Signatures PTY Ltd. Methods for genome amplification
US8808991B2 (en) 2003-09-02 2014-08-19 Keygene N.V. Ola-based methods for the detection of target nucleic avid sequences
US7846693B2 (en) 2003-09-04 2010-12-07 Human Genetic Signatures Pty. Ltd. Nucleic acid detection assay
JP2007530051A (en) 2004-03-24 2007-11-01 アプレラ コーポレイション Ligation and amplification reactions to determine target molecules
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2006012468A2 (en) * 2004-07-23 2006-02-02 (Osi) Eyetech, Inc. Detection of oligonuleotides by dual hybridization
EP1794173B1 (en) 2004-09-10 2010-08-04 Human Genetic Signatures PTY Ltd Amplification blocker comprising intercalating nucleic acids (ina) containing intercalating pseudonucleotides (ipn)
US7833942B2 (en) 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
CA2609218C (en) 2005-05-26 2016-10-11 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
CA2625909C (en) * 2005-08-01 2014-04-08 National Research Council Of Canada Development of pcr primers and primer mixtures for amplification of cnp60 target sequences
US8343738B2 (en) 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
EP1969137B1 (en) 2005-11-22 2011-10-05 Stichting Dienst Landbouwkundig Onderzoek Multiplex nucleic acid detection
EP1991698B1 (en) 2006-03-01 2013-12-18 Keygene N.V. High throughput sequence-based detection of snps using ligation assays
WO2008118998A2 (en) * 2007-03-27 2008-10-02 Primera Biosystems Inc. Method for multiplex detection and quantitation of nucleic acids
US8685675B2 (en) 2007-11-27 2014-04-01 Human Genetic Signatures Pty. Ltd. Enzymes for amplification and copying bisulphite modified nucleic acids
EP2113574A1 (en) * 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
CN101532014B (en) * 2008-12-12 2010-12-22 深圳华大基因研究院 Public connectors for connecting amplification edges of target genome area edges and connecting method
KR101306988B1 (en) * 2011-03-16 2013-09-10 연세대학교 산학협력단 Assembly Methods of Multiple Target Loci to a Single Nucleotide Sequence
DK2729580T3 (en) 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
EP2753710B1 (en) 2011-09-07 2017-01-11 Human Genetic Signatures Pty Ltd Molecular detection assay
WO2013106807A1 (en) 2012-01-13 2013-07-18 Curry John D Scalable characterization of nucleic acids by parallel sequencing
JP6234463B2 (en) * 2012-09-10 2017-11-22 ジーンスカイ ダイアグノスティクス（スーツォウ） インコーポレーテッドＧｅｎｅｓｋｙ Ｄｉａｇｎｏｓｔｉｃｓ （Ｓｕｚｈｏｕ） Ｉｎｃ． Nucleic acid multiplex analysis method
CA2960859A1 (en) * 2014-09-17 2016-03-24 Theranos, Inc. Hybrid multi-step nucleic acid amplification
EP3307908B1 (en) 2015-06-09 2019-09-11 Life Technologies Corporation Methods for molecular tagging
US11118216B2 (en) 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
AU2019207910A1 (en) * 2018-01-12 2019-11-21 Stem Arts Projects, Llc Reaction condition composition for circularizing oligonucleotide probes
AU2019287163B2 (en) 2018-06-12 2025-08-21 Keygene N.V. Nucleic acid amplification method
CA3127572A1 (en) 2019-02-21 2020-08-27 Keygene N.V. Genotyping of polyploids
WO2025205770A1 (en) * 2024-03-26 2025-10-02 国立大学法人 東京大学 Recombinant plasmid containing stuffer dna

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022623A1 (en) * 1994-02-16 1995-08-24 Ulf Landegren Circularizing nucleic acid probe able to interlock with a target sequence through catenation
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US6156178A (en) * 1999-07-13 2000-12-05 Molecular Dynamics, Inc. Increased throughput analysis of small compounds using multiple temporally spaced injections
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
US6221603B1 (en) * 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis

Family Cites Families (3)

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EP2369007B1 (en) * 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
EP1130113A1 (en) * 2000-02-15 2001-09-05 Johannes Petrus Schouten Multiplex ligation dependent amplification assay
EP1633888B1 (en) * 2003-06-17 2009-03-25 Keygene N.V. Means and method for the detection of target nucleotide sequences using ligation assays with improved oligonucleotide probe pairs

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995022623A1 (en) * 1994-02-16 1995-08-24 Ulf Landegren Circularizing nucleic acid probe able to interlock with a target sequence through catenation
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US6156178A (en) * 1999-07-13 2000-12-05 Molecular Dynamics, Inc. Increased throughput analysis of small compounds using multiple temporally spaced injections
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof
US6221603B1 (en) * 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BANER JOHAN ET AL: "More keys to padlock probes: Mechanisms for high-throughput nucleic acid analysis.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 12, no. 1, February 2001 (2001-02-01), pages 11 - 15, XP002244472, ISSN: 0958-1669 *
GROSSMAN ET AL: "HIGH-DENSITY MULTIPLEX DETECTION OF NUCLEIC ACID SEQUENCES: OLIGONUCLEOTIDE LIGATION ASSAY AND SEQUENCE-CODED SEPARATION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 22, no. 21, 1994, pages 4527 - 4534, XP002144258, ISSN: 0305-1048 *
LANDEGREN U ET AL: "A LIGASE-MEDIATED GENE DETECTION TECHNIQUE", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 241, no. 4869, 26 August 1988 (1988-08-26), pages 1077 - 1080, XP000676556, ISSN: 0036-8075 *
SHI MICHAEL M: "Enabling large-scale pharmacogenetic studies by high-throughput mutation detection and genotyping technologies", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 47, no. 2, February 2000 (2000-02-01), pages 164 - 172, XP002197957, ISSN: 0009-9147 *
THOMAS D C ET AL: "AMPLIFICATION OF PADLOCK PROBES FOR DNA DIAGNOSTICS BY CASCADE ROLLING CIRCLE AMPLIFICATION OR THE POLYMERASE CHAIN REACTION", ARCHIVES OF PATHOLOGY, AMERICAN MEDICAL ASSOCIATION, CHICAGO, IL,, US, vol. 123, no. 12, December 1999 (1999-12-01), pages 1170 - 1176, XP008001739, ISSN: 0003-9985 *

Also Published As

Publication number Publication date
AU2002360222A1 (en) 2003-06-30
CA2470356A1 (en) 2003-06-26
JP2005512549A (en) 2005-05-12
JP4226476B2 (en) 2009-02-18
WO2003052141A2 (en) 2003-06-26
ATE358186T1 (en) 2007-04-15
AU2002360223A1 (en) 2003-06-30
WO2003052140A2 (en) 2003-06-26
US7498131B2 (en) 2009-03-03
WO2003052142A2 (en) 2003-06-26
ES2284967T3 (en) 2007-11-16
WO2003052141A9 (en) 2003-12-18
AU2002360223B2 (en) 2006-04-27
AU2002359090A1 (en) 2003-06-30
EP1453978B1 (en) 2007-03-28
WO2003052141A3 (en) 2004-02-26
EP1319718A1 (en) 2003-06-18
WO2003052140A3 (en) 2003-11-13
EP1453978A2 (en) 2004-09-08
DE60219199T2 (en) 2008-01-03
US20060121458A1 (en) 2006-06-08
DE60219199D1 (en) 2007-05-10
EP1453978B9 (en) 2007-09-12

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