WO2003066897A3 - Specific multiplex analysis of nucleic acids - Google Patents

Specific multiplex analysis of nucleic acids Download PDF

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Publication number
WO2003066897A3
WO2003066897A3 PCT/EP2003/001276 EP0301276W WO03066897A3 WO 2003066897 A3 WO2003066897 A3 WO 2003066897A3 EP 0301276 W EP0301276 W EP 0301276W WO 03066897 A3 WO03066897 A3 WO 03066897A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
diagnostic
primers
nucleic acid
acid sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/001276
Other languages
French (fr)
Other versions
WO2003066897A2 (en
Inventor
Rudolf Zirwes
Elke Koppers
Imke Gores
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Revvity Cellular Technologies GmbH
Original Assignee
Evotec Technologies GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Technologies GmbH filed Critical Evotec Technologies GmbH
Priority to DK03704579T priority Critical patent/DK1474529T3/en
Priority to US10/503,436 priority patent/US20050208491A1/en
Priority to AU2003206869A priority patent/AU2003206869A1/en
Priority to DE60324253T priority patent/DE60324253D1/en
Priority to EP03704579A priority patent/EP1474529B1/en
Publication of WO2003066897A2 publication Critical patent/WO2003066897A2/en
Publication of WO2003066897A3 publication Critical patent/WO2003066897A3/en
Anticipated expiration legal-status Critical
Priority to US11/979,494 priority patent/US20090029364A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

A method of detecting the presence or absence of variant nucleotides within target nucleic acid sequences, said method comprising the use of at least one pair of target-specific amplification primers capable of hybridizing to target nucleic acid sequences, and the use of at least one set of diagnostic primers, each set consisting of at least two types of diagnostic primers capable of hybridizing to corresponding target nucleic acid sequences 3´-relative to said amplification primers, such that each set of diagnostic primers is semi-nested relative to the corresponding pair of amplification primers. Additionally, the use of said method for genotyping, for diagnostic screening, for assaying the cytosine methylation status and for quantification of nucleic acids is disclosed.
PCT/EP2003/001276 2002-02-08 2003-02-10 Specific multiplex analysis of nucleic acids Ceased WO2003066897A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DK03704579T DK1474529T3 (en) 2002-02-08 2003-02-10 Specific multiplex analysis of nucleic acids
US10/503,436 US20050208491A1 (en) 2002-02-08 2003-02-10 Specific multiplex analysis of nucleic acids
AU2003206869A AU2003206869A1 (en) 2002-02-08 2003-02-10 Specific multiplex analysis of nucleic acids
DE60324253T DE60324253D1 (en) 2002-02-08 2003-02-10 SPECIFIC MULTIPLEX ANALYSIS OF NUCLEIC ACIDS
EP03704579A EP1474529B1 (en) 2002-02-08 2003-02-10 Specific multiplex analysis of nucleic acids
US11/979,494 US20090029364A1 (en) 2002-02-08 2007-11-05 Specific multiplex analysis of nucleic acids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US35451802P 2002-02-08 2002-02-08
EP02002854 2002-02-08
EP02002854.4 2002-02-08
US60/354,518 2002-02-08

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/979,494 Division US20090029364A1 (en) 2002-02-08 2007-11-05 Specific multiplex analysis of nucleic acids

Publications (2)

Publication Number Publication Date
WO2003066897A2 WO2003066897A2 (en) 2003-08-14
WO2003066897A3 true WO2003066897A3 (en) 2004-01-22

Family

ID=56290378

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/001276 Ceased WO2003066897A2 (en) 2002-02-08 2003-02-10 Specific multiplex analysis of nucleic acids

Country Status (5)

Country Link
EP (1) EP1474529B1 (en)
AT (1) ATE412068T1 (en)
AU (1) AU2003206869A1 (en)
DK (1) DK1474529T3 (en)
WO (1) WO2003066897A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101078977B1 (en) 2002-11-07 2011-11-01 세키스이 메디칼 가부시키가이샤 Method of detecting gene mutation
NZ546903A (en) 2003-10-13 2009-06-26 Genaco Biomedical Products Inc Method and kit for primer based amplification of nucleic acids
JP4777631B2 (en) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ Nucleic acid amplification analysis method and apparatus
CA2610863C (en) 2005-06-07 2017-01-17 Eragen Biosciences, Inc. Methods for detection and typing of nucleic acids
ES2494922T3 (en) * 2005-09-01 2014-09-16 Ausdiagnostics Pty Ltd. Methods for amplification, quantification and identification of nucleic acids
WO2017087735A1 (en) * 2015-11-18 2017-05-26 Millennium Pharmaceuticals, Inc. Method for treating crohn's disease
CN111235261B (en) * 2019-12-20 2022-11-04 江苏伟禾生物科技有限公司 Kit for detecting human platelet-specific antigen HPA 1-29 genotyping

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1061135A2 (en) * 1999-06-17 2000-12-20 Becton Dickinson and Company Methods and oligonucleotides for detecting nucleic acid sequence variations

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1061135A2 (en) * 1999-06-17 2000-12-20 Becton Dickinson and Company Methods and oligonucleotides for detecting nucleic acid sequence variations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOLDT B ET AL: "ONE-TUBE METHOD FOR COMPLETE HPA-1 GENOTYPING BY PCR USING SEQUENCE-SPECIFIC PRIMERS", BRITISH JOURNAL OF HAEMATOLOGY, OXFORD, GB, vol. 99, no. 4, December 1997 (1997-12-01), pages 968 - 973, XP001096153, ISSN: 0007-1048 *
HAFF L A: "IMPROVED QUANTITATIVE PCR USING NESTED PRIMERS", PCR METHODS & APPLICATIONS, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 3, no. 6, 1 June 1994 (1994-06-01), pages 332 - 337, XP000561192, ISSN: 1054-9803 *
KWOK S ET AL: "Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 18, no. 4, 25 February 1990 (1990-02-25), pages 999 - 1005, XP002220845, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
AU2003206869A1 (en) 2003-09-02
WO2003066897A2 (en) 2003-08-14
EP1474529A2 (en) 2004-11-10
ATE412068T1 (en) 2008-11-15
EP1474529B1 (en) 2008-10-22
DK1474529T3 (en) 2009-02-23

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