WO2003072805A3 - Recombinase polymerase amplification - Google Patents

Recombinase polymerase amplification Download PDF

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Publication number
WO2003072805A3
WO2003072805A3 PCT/US2003/005492 US0305492W WO03072805A3 WO 2003072805 A3 WO2003072805 A3 WO 2003072805A3 US 0305492 W US0305492 W US 0305492W WO 03072805 A3 WO03072805 A3 WO 03072805A3
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WO
WIPO (PCT)
Prior art keywords
dna
amplification
polymerase amplification
recombinase polymerase
recombinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/005492
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French (fr)
Other versions
WO2003072805A2 (en
Inventor
Niall A Armes
Derek L Stemple
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TwistDix Inc
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TwistDix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TwistDix Inc filed Critical TwistDix Inc
Priority to EP03711216A priority Critical patent/EP1499738B1/en
Priority to JP2003571485A priority patent/JP4340543B2/en
Priority to CA2476481A priority patent/CA2476481C/en
Priority to DE60322044T priority patent/DE60322044D1/en
Priority to DK03711216T priority patent/DK1499738T3/en
Priority to AU2003215391A priority patent/AU2003215391B2/en
Publication of WO2003072805A2 publication Critical patent/WO2003072805A2/en
Anticipated expiration legal-status Critical
Publication of WO2003072805A3 publication Critical patent/WO2003072805A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
    • G06F16/90—Details of database functions independent of the retrieved data types
    • G06F16/95—Retrieval from the web
    • G06F16/951—Indexing; Web crawling techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F16/00—Information retrieval; Database structures therefor; File system structures therefor
    • G06F16/90—Details of database functions independent of the retrieved data types
    • G06F16/95—Retrieval from the web
    • G06F16/957—Browsing optimisation, e.g. caching or content distillation
    • G06F16/9574—Browsing optimisation, e.g. caching or content distillation of access to content, e.g. by caching

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Databases & Information Systems (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Data Mining & Analysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of the bacterial RecA and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed method has the advantage of not requiring tehrmocycling or thermophilic enzymes. Further, the improved processivity of the dislosed methods allow amplification of DNA up to hundreds of megabases in length.
PCT/US2003/005492 2002-02-21 2003-02-21 Recombinase polymerase amplification Ceased WO2003072805A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP03711216A EP1499738B1 (en) 2002-02-21 2003-02-21 Recombinase polymerase amplification
JP2003571485A JP4340543B2 (en) 2002-02-21 2003-02-21 Recombinase polymerase amplification
CA2476481A CA2476481C (en) 2002-02-21 2003-02-21 Recombinase polymerase amplification
DE60322044T DE60322044D1 (en) 2002-02-21 2003-02-21 Recombinase Polymerase Amplification
DK03711216T DK1499738T3 (en) 2002-02-21 2003-02-21 Recombinase polymerease amplification
AU2003215391A AU2003215391B2 (en) 2002-02-21 2003-02-21 Recombinase Polymerase Amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35856302P 2002-02-21 2002-02-21
US60/358,563 2002-02-21

Publications (2)

Publication Number Publication Date
WO2003072805A2 WO2003072805A2 (en) 2003-09-04
WO2003072805A3 true WO2003072805A3 (en) 2004-11-25

Family

ID=27765964

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/005492 Ceased WO2003072805A2 (en) 2002-02-21 2003-02-21 Recombinase polymerase amplification

Country Status (11)

Country Link
US (7) US7270981B2 (en)
EP (4) EP1992706B1 (en)
JP (4) JP4340543B2 (en)
AT (1) ATE400663T1 (en)
AU (1) AU2003215391B2 (en)
CA (1) CA2476481C (en)
DE (1) DE60322044D1 (en)
DK (1) DK1499738T3 (en)
ES (1) ES2310237T3 (en)
PT (1) PT1499738E (en)
WO (1) WO2003072805A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
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US9562263B2 (en) 2007-07-14 2017-02-07 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids

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