WO2003083477A3 - Active solid support and method for surface immobilization of combinatorial compounds or libraries - Google Patents

Active solid support and method for surface immobilization of combinatorial compounds or libraries Download PDF

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Publication number
WO2003083477A3
WO2003083477A3 PCT/HU2003/000025 HU0300025W WO03083477A3 WO 2003083477 A3 WO2003083477 A3 WO 2003083477A3 HU 0300025 W HU0300025 W HU 0300025W WO 03083477 A3 WO03083477 A3 WO 03083477A3
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WO
WIPO (PCT)
Prior art keywords
solid support
group
active solid
reacted
drug
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/HU2003/000025
Other languages
French (fr)
Other versions
WO2003083477A2 (en
Inventor
Ferenc Darvas
Laszlo Uerge
Agota Bucsai
Mariann Beres
Gyoergy Dorman
Peter Krajcsi
Lajos Goedoerhazy
Istvan Bagyi
Laszlo Puskas
Laszlo Hackler
Agnes Zvara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AMRI Hungary Zrt
Original Assignee
Comgenex RT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Comgenex RT filed Critical Comgenex RT
Priority to AU2003226574A priority Critical patent/AU2003226574A1/en
Priority to EP03745343A priority patent/EP1493031A2/en
Publication of WO2003083477A2 publication Critical patent/WO2003083477A2/en
Publication of WO2003083477A3 publication Critical patent/WO2003083477A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The subject of the invention is a method for production of an active solid support suitable for anchoring drug molecules through covalent bonds, using the following methods a.) a solid support prepared in advance, preferably by pre-treatment in the presence of strong bases or acids, then reacted with a polyamine-functionalized alcoxy-silane derivative, preferably 3-[2-(2-aminoethylamino)ethylamino]propyl trimethoxy-silane, followed by washing and drying, b1) the solid support obtained in the procedure described above in Section a.) will be reacted with (I) where Z: = bond or a -CO or -CNR 1 group and group 121 is an alkyl, aralkyl or aryl group; B: = bond or a -(CH2)n- or -(CH2)n O-(CH2)n or O-(CH2)n- or -NH(CH2)n- group; A: = a branched or unbranched alkyl or substituted aryl or aralkyl group containing 1-5 carbon atoms; D: = a halogen or -O-A group and n = 0, 1, 2, ... integer - with a bi-functional reagent, preferably reacted with acryloyl/chloride or 1,4-butanediol diacrylate in the presence of an organic or inorganic scavenger or b2) the support prepared using the method described in part (a) is reacted with a bi-functional reagent, preferably 1,4-butanediol diglicidyl-ether or epichlorohydrin, in the presence of an organic or inorganic scavenger containing terminal epoxy groups and an amino-group reactive functional subgroup. The invention also includes the active solid support produced using the several methods described above, as well as application of the active solid support described above for production of chemical microarrays and combinatorial chemical microarrays. The invention further comprises a method for immobilization of what have been termed organic molecules of low molecular weight, having a size less than 1500 Da, preferably between 250 and 750 Da. This is achieved by anchoring an unmodified drug or drug candidate molecule to the active solid support described above by adding the appropriate functional groups of the drug or drug candidate molecule, preferably executed using a micron-sized robotized binding technique.
PCT/HU2003/000025 2002-03-29 2003-03-28 Active solid support and method for surface immobilization of combinatorial compounds or libraries Ceased WO2003083477A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003226574A AU2003226574A1 (en) 2002-03-29 2003-03-28 Active solid support and method for surface immobilization of combinatorial compounds or libraries
EP03745343A EP1493031A2 (en) 2002-03-29 2003-03-28 Active solid support and method for surface immobilization of combinatorial compounds or libraries

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HUP0201091 2002-03-29
HU0201091A HU226993B1 (en) 2002-03-29 2002-03-29 Novel active carrier and process for immobilization of combinatorial compounds and compound libraries

Publications (2)

Publication Number Publication Date
WO2003083477A2 WO2003083477A2 (en) 2003-10-09
WO2003083477A3 true WO2003083477A3 (en) 2004-05-06

Family

ID=89980305

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU2003/000025 Ceased WO2003083477A2 (en) 2002-03-29 2003-03-28 Active solid support and method for surface immobilization of combinatorial compounds or libraries

Country Status (4)

Country Link
EP (1) EP1493031A2 (en)
AU (1) AU2003226574A1 (en)
HU (1) HU226993B1 (en)
WO (1) WO2003083477A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7217512B2 (en) 2002-05-09 2007-05-15 Corning Incorporated Reagent and method for attaching target molecules to a surface
US7723126B2 (en) * 2004-03-24 2010-05-25 Wisconsin Alumni Research Foundation Plasma-enhanced functionalization of inorganic oxide surfaces
US20060147943A1 (en) * 2004-12-30 2006-07-06 Lewis Mark A Substrates having pendant epoxide groups for binding biomolecules and methods of making and using thereof
US7396676B2 (en) 2005-05-31 2008-07-08 Agilent Technologies, Inc. Evanescent wave sensor with attached ligand
US20070099180A1 (en) * 2005-10-31 2007-05-03 Robotti Karla M Evanescent wave sensor with attached ligand
HUP0600668A2 (en) 2006-08-22 2008-02-28 Avicor Kft Active carrier, process for producing thereof and the use of thereof
US8029902B2 (en) 2006-12-11 2011-10-04 Wisconsin Alumni Research Foundation Plasma-enhanced functionalization of substrate surfaces with quaternary ammonium and quaternary phosphonium groups

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5919523A (en) * 1995-04-27 1999-07-06 Affymetrix, Inc. Derivatization of solid supports and methods for oligomer synthesis
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
WO2001001143A2 (en) * 1999-06-25 2001-01-04 Motorola Inc. Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5919523A (en) * 1995-04-27 1999-07-06 Affymetrix, Inc. Derivatization of solid supports and methods for oligomer synthesis
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
WO2001001143A2 (en) * 1999-06-25 2001-01-04 Motorola Inc. Attachment of biomolecule to a polymeric solid support by cycloaddition of a linker

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BLACKBURN CHRISTOPHER: "Polymer supports for solid-phase organic synthesis.", BIOPOLYMERS, vol. 47, no. 5, 25 January 1998 (1998-01-25), pages 311 - 351, XP002255078, ISSN: 0006-3525 *
REHMAN F ET AL: "Immobilization of acrylamide-modified oligonucleotides by co-polymerization", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 2, 15 February 1999 (1999-02-15), pages 649 - 655, XP002155808, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
HU226993B1 (en) 2010-04-28
HUP0201091A3 (en) 2007-08-28
EP1493031A2 (en) 2005-01-05
AU2003226574A8 (en) 2003-10-13
HU0201091D0 (en) 2002-06-29
WO2003083477A2 (en) 2003-10-09
HUP0201091A2 (en) 2003-12-29
AU2003226574A1 (en) 2003-10-13

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