WO2003085094A3 - Solid phase methods for polynucleotide production - Google Patents

Solid phase methods for polynucleotide production Download PDF

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Publication number
WO2003085094A3
WO2003085094A3 PCT/US2003/009814 US0309814W WO03085094A3 WO 2003085094 A3 WO2003085094 A3 WO 2003085094A3 US 0309814 W US0309814 W US 0309814W WO 03085094 A3 WO03085094 A3 WO 03085094A3
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WO
WIPO (PCT)
Prior art keywords
oligonucleotide
solid support
target polynucleotide
solid phase
annealed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/009814
Other languages
French (fr)
Other versions
WO2003085094A2 (en
Inventor
Hsing-Yeh Parker
John T Mulligan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Blue Heron Biotechnology Inc
Original Assignee
Blue Heron Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Blue Heron Biotechnology Inc filed Critical Blue Heron Biotechnology Inc
Priority to EP03714474A priority Critical patent/EP1499626A2/en
Priority to AU2003218470A priority patent/AU2003218470A1/en
Priority to CA002478983A priority patent/CA2478983A1/en
Priority to JP2003582273A priority patent/JP2005521419A/en
Publication of WO2003085094A2 publication Critical patent/WO2003085094A2/en
Anticipated expiration legal-status Critical
Publication of WO2003085094A3 publication Critical patent/WO2003085094A3/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.
PCT/US2003/009814 2002-04-01 2003-04-01 Solid phase methods for polynucleotide production Ceased WO2003085094A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03714474A EP1499626A2 (en) 2002-04-01 2003-04-01 Solid phase methods for polynucleotide production
AU2003218470A AU2003218470A1 (en) 2002-04-01 2003-04-01 Solid phase methods for polynucleotide production
CA002478983A CA2478983A1 (en) 2002-04-01 2003-04-01 Solid phase methods for polynucleotide production
JP2003582273A JP2005521419A (en) 2002-04-01 2003-04-01 Solid phase method for polynucleotide production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US36947802P 2002-04-01 2002-04-01
US60/369,478 2002-04-01
US39052202P 2002-06-20 2002-06-20
US60/390,522 2002-06-20

Publications (2)

Publication Number Publication Date
WO2003085094A2 WO2003085094A2 (en) 2003-10-16
WO2003085094A3 true WO2003085094A3 (en) 2004-11-25

Family

ID=28794364

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/009814 Ceased WO2003085094A2 (en) 2002-04-01 2003-04-01 Solid phase methods for polynucleotide production

Country Status (6)

Country Link
US (1) US20030228602A1 (en)
EP (1) EP1499626A2 (en)
JP (1) JP2005521419A (en)
AU (1) AU2003218470A1 (en)
CA (1) CA2478983A1 (en)
WO (1) WO2003085094A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
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US7323320B2 (en) 2002-09-12 2008-01-29 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

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US7932025B2 (en) * 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
JP2007508825A (en) * 2003-10-15 2007-04-12 ブルー ヘロン バイオテクノロジー インコーポレイテッド Solid phase method for polynucleotide production
EP1817413B1 (en) * 2004-11-11 2012-01-04 Modular Genetics, Inc. Oligonucleotide ladder assembly and system for generating molecular diversity
US20070148681A1 (en) * 2005-12-16 2007-06-28 Gene Oracle, Inc. Gene synthesis kit
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
EP2285974A2 (en) * 2008-05-14 2011-02-23 British Columbia Cancer Agency Branch Gene synthesis by convergent assembly of oligonucleotide subsets
CA2747535C (en) 2008-12-18 2020-01-14 Iti Scotland Limited Method for assembly of polynucleic acid sequences
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
DE102010056289A1 (en) 2010-12-24 2012-06-28 Geneart Ag Process for the preparation of reading frame correct fragment libraries
LT2944693T (en) 2011-08-26 2019-08-26 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN111876409A (en) 2012-04-24 2020-11-03 Gen9股份有限公司 Method for sorting nucleic acids and multiplex preparations in vitro cloning
AU2013280661A1 (en) 2012-06-25 2015-01-22 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
TWI707038B (en) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 De novo synthesized gene libraries
WO2015089053A1 (en) * 2013-12-09 2015-06-18 Integrated Dna Technologies, Inc. Long nucleic acid sequences containing variable regions
CA2965485A1 (en) * 2014-10-21 2016-04-28 Martin R. Schiller Methods and compositions for screening molecular function comprising chimeric minimotifs
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
WO2016179602A1 (en) * 2015-05-07 2016-11-10 The Regents Of The University Of California Solid phase sequence-independent nucleic acid assembly
WO2017015348A1 (en) * 2015-07-20 2017-01-26 The Regents Of The University Of California Sequence-independent nucleic acid assembly
KR20180050411A (en) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 Oligonucleotide mutant library and its synthesis
KR102794025B1 (en) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 Flexible substrates for nucleic acid synthesis
CN115920796A (en) 2015-12-01 2023-04-07 特韦斯特生物科学公司 Functionalized surfaces and their preparation
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
JP6854340B2 (en) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション Denovo Synthesized Nucleic Acid Library
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
KR102514213B1 (en) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 Immune synaptic variant library and its synthesis
CN110892485B (en) 2017-02-22 2024-03-22 特韦斯特生物科学公司 Nucleic acid-based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EP3681637A4 (en) * 2017-09-11 2021-06-02 Synthego Corporation Biopolymer synthesis system and method
CN111566125A (en) 2017-09-11 2020-08-21 特韦斯特生物科学公司 GPCR binding proteins and synthesis thereof
CN111565834B (en) 2017-10-20 2022-08-26 特韦斯特生物科学公司 Heated nanopores for polynucleotide synthesis
EP3733846A4 (en) * 2017-12-27 2021-10-27 Toray Industries, Inc. Method for recovering nucleic acid
KR102804057B1 (en) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 DNA-based digital information storage
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN113766930B (en) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Variant nucleic acid libraries for GLP1 receptors
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
JP2022548309A (en) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション CRTH2 variant nucleic acid library
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
IL293670A (en) 2019-12-09 2022-08-01 Twist Bioscience Corp Variant nucleic acid libraries for adenosine receptors
WO2022221853A1 (en) 2021-04-13 2022-10-20 Elegen Corp. Methods and compositions for cell-free cloning

Citations (1)

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Publication number Priority date Publication date Assignee Title
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US5437979A (en) * 1989-07-24 1995-08-01 Beckman Instruments, Inc. Solid phase system for sequential reactions
US5705344A (en) * 1996-03-14 1998-01-06 Tularik, Inc. High-throughput screening assay for inhibitors of nucleic acid helicases
US6277622B1 (en) * 1997-08-11 2001-08-21 The University Of Sydney Synthetic polynucleotides
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7323320B2 (en) 2002-09-12 2008-01-29 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Also Published As

Publication number Publication date
JP2005521419A (en) 2005-07-21
WO2003085094A2 (en) 2003-10-16
CA2478983A1 (en) 2003-10-16
EP1499626A2 (en) 2005-01-26
US20030228602A1 (en) 2003-12-11
AU2003218470A1 (en) 2003-10-20

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