WO2003087341A3 - Targeted chromosomal mutagenesis using zinc finger nucleases - Google Patents

Targeted chromosomal mutagenesis using zinc finger nucleases Download PDF

Info

Publication number
WO2003087341A3
WO2003087341A3 PCT/US2003/002012 US0302012W WO03087341A3 WO 2003087341 A3 WO2003087341 A3 WO 2003087341A3 US 0302012 W US0302012 W US 0302012W WO 03087341 A3 WO03087341 A3 WO 03087341A3
Authority
WO
WIPO (PCT)
Prior art keywords
genetic material
present
provides
targeted
mutagenesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/002012
Other languages
French (fr)
Other versions
WO2003087341A2 (en
Inventor
Dana Carroll
Marina Bibikova
Gary N Drews
Kent Gregory Golic
Mary M Golic
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation Inc
Original Assignee
University of Utah Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US10/502,565 priority Critical patent/US8106255B2/en
Priority to BRPI0307383A priority patent/BRPI0307383B1/en
Priority to CA 2474486 priority patent/CA2474486C/en
Priority to EP03746527A priority patent/EP1476547B1/en
Priority to AU2003251286A priority patent/AU2003251286B2/en
Priority to JP2003584285A priority patent/JP4968498B2/en
Priority to DE2003610202 priority patent/DE60310202T2/en
Application filed by University of Utah Research Foundation Inc filed Critical University of Utah Research Foundation Inc
Priority to BR0307383A priority patent/BR0307383A/en
Publication of WO2003087341A2 publication Critical patent/WO2003087341A2/en
Publication of WO2003087341A3 publication Critical patent/WO2003087341A3/en
Anticipated expiration legal-status Critical
Priority to US13/314,490 priority patent/US9145565B2/en
Priority to US14/867,816 priority patent/US20160076045A1/en
Priority to US16/778,193 priority patent/US20200231979A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/60—New or modified breeds of invertebrates
    • A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
    • A01K67/65—Genetically modified arthropods
    • A01K67/68—Genetically modified insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Catalysts (AREA)
  • Electrolytic Production Of Metals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Inorganic Compounds Of Heavy Metals (AREA)

Abstract

The present invention provides for a method or methods of targeted genetic recombination or mutagenesis in a host cell or organism, and compositions useful for carrying out the method. The targeting method of the present invention exploits endogenous cellular mechanisms for homologous recombination and repair of double stranded breaks in genetic material. The present provides numerous improvements over previous mutagenesis methods, such advantages including that the method is generally applicable to a wide variety of organisms, the method is targeted so that the disadvantages associated with random insertion of DNA in to host genetic material are eliminated, and certain embodiments require relatively little manipulation of the host genetic material for success. Additionally, it provides a method that produces organisms with specific gene modifications in a short period of time.
PCT/US2003/002012 2002-01-23 2003-01-22 Targeted chromosomal mutagenesis using zinc finger nucleases Ceased WO2003087341A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
JP2003584285A JP4968498B2 (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenesis using zinc finger nuclease
BRPI0307383A BRPI0307383B1 (en) 2002-01-23 2003-01-22 directed genetic recombination method in host plant cell
CA 2474486 CA2474486C (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenesis using zinc finger nucleases
EP03746527A EP1476547B1 (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenesis using zinc finger nucleases
AU2003251286A AU2003251286B2 (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenesis using zinc finger nucleases
DE2003610202 DE60310202T2 (en) 2002-01-23 2003-01-22 TARGETED CHROMOSOMAL MUTAGENESIS WITH ZINC FINGERUCLEASES
BR0307383A BR0307383A (en) 2002-01-23 2003-01-22 Target chromosomal mutagenesis using zinc branch nucleases
US10/502,565 US8106255B2 (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenasis using zinc finger nucleases
US13/314,490 US9145565B2 (en) 2002-01-23 2011-12-08 Targeted chromosomal mutagenesis using zinc finger nucleases
US14/867,816 US20160076045A1 (en) 2002-01-23 2015-09-28 Targeted chromosomal mutagenesis using zinc finger nucleases
US16/778,193 US20200231979A1 (en) 2002-01-23 2020-01-31 Targeted chromosomal mutagenesis using zinc finger nucleases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35103502P 2002-01-23 2002-01-23
US60/351,035 2002-01-23

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US10502565 A-371-Of-International 2003-01-22
US10/502,565 A-371-Of-International US8106255B2 (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenasis using zinc finger nucleases
US13/314,490 Continuation US9145565B2 (en) 2002-01-23 2011-12-08 Targeted chromosomal mutagenesis using zinc finger nucleases

Publications (2)

Publication Number Publication Date
WO2003087341A2 WO2003087341A2 (en) 2003-10-23
WO2003087341A3 true WO2003087341A3 (en) 2004-04-29

Family

ID=29250450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/002012 Ceased WO2003087341A2 (en) 2002-01-23 2003-01-22 Targeted chromosomal mutagenesis using zinc finger nucleases

Country Status (10)

Country Link
US (4) US8106255B2 (en)
EP (1) EP1476547B1 (en)
JP (2) JP4968498B2 (en)
CN (1) CN100575485C (en)
AT (1) ATE347593T1 (en)
AU (1) AU2003251286B2 (en)
BR (2) BRPI0307383B1 (en)
CA (1) CA2474486C (en)
DE (1) DE60310202T2 (en)
WO (1) WO2003087341A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591592B2 (en) 2020-04-24 2023-02-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers

Families Citing this family (457)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE347593T1 (en) * 2002-01-23 2006-12-15 Univ Utah Res Found TARGETED CHROMOSOMAL MUTAGENESIS WITH ZINC FINGER NUCLEASES
EP1504092B2 (en) * 2002-03-21 2014-06-25 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US9447434B2 (en) 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20080311095A1 (en) * 2007-05-23 2008-12-18 Sangamo Biosciences, Inc. Methods and compositions for increased transgene expression
EP2927318B1 (en) * 2003-08-08 2020-05-20 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
CA2539439C (en) 2003-09-19 2012-10-23 Sangamo Biosciences, Inc. Engineered zinc finger proteins for regulation of gene expression
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
AU2005233583B2 (en) 2004-04-08 2011-02-03 Sangamo Therapeutics, Inc. Methods and compositions for modulating cardiac contractility
KR20070060115A (en) 2004-09-16 2007-06-12 상가모 바이오사이언스 인코포레이티드 Compositions and Methods for Protein Production
AU2012245168B2 (en) * 2005-07-26 2014-12-11 Sangamo Therapeutics, Inc. Targeted Integration and Expression of Exogenous Nucleic Acid Sequences
EP1913149A4 (en) * 2005-07-26 2009-08-05 Sangamo Biosciences Inc TARGETED INTEGRATION AND EXPRESSION OF EXOGENOUS NUCLEIC ACID SEQUENCES
DK3246403T3 (en) 2005-10-18 2020-10-26 Prec Biosciences RATIONALLY DESIGNED MEGANUCLEASES WITH CHANGED SEQUENCE SPECIFICITY AND DNA BINDING AFFINITY
US20070154989A1 (en) * 2006-01-03 2007-07-05 The Scripps Research Institute Zinc finger domains specifically binding agc
US20070266449A1 (en) * 2006-05-12 2007-11-15 Zivin Robert A Generation of animal models
WO2007136685A2 (en) * 2006-05-19 2007-11-29 Sangamo Biosciences, Inc. Methods and compositions for inactivation of dihydrofolate reductase
EP2027262B1 (en) * 2006-05-25 2010-03-31 Sangamo Biosciences Inc. Variant foki cleavage half-domains
US7951925B2 (en) 2006-05-25 2011-05-31 Sangamo Biosciences, Inc. Methods and compositions for gene inactivation
JP2010500029A (en) * 2006-08-11 2010-01-07 ダウ アグロサイエンシィズ エルエルシー Zinc finger nuclease-mediated homologous recombination
WO2008060510A2 (en) 2006-11-13 2008-05-22 Sangamo Biosciences, Inc. Zinc finger nuclease for targeting the human glucocorticoid receptor locus
CA2669746C (en) * 2006-12-14 2017-05-16 Dow Agrosciences Llc Optimized non-canonical zinc finger proteins
EP2155868A2 (en) * 2007-04-19 2010-02-24 Codon Devices, Inc Engineered nucleases and their uses for nucleic acid assembly
US8110379B2 (en) 2007-04-26 2012-02-07 Sangamo Biosciences, Inc. Targeted integration into the PPP1R12C locus
CA2691440A1 (en) 2007-06-29 2009-01-08 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
AU2008275649B2 (en) 2007-07-12 2013-09-05 Sangamo Therapeutics, Inc. Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT 8) gene expression
US11235026B2 (en) 2007-09-27 2022-02-01 Sangamo Therapeutics, Inc. Methods and compositions for modulating PD1
AU2008305590A1 (en) * 2007-09-27 2009-04-02 Sangamo Therapeutics, Inc. Genomic editing in zebrafish using zinc finger nucleases
ES2585157T3 (en) * 2007-09-27 2016-10-04 Dow Agrosciences Llc Designer zinc finger proteins that target the 5-enolpiruvil shikimato-3-phosphate synthase genes
US8563314B2 (en) 2007-09-27 2013-10-22 Sangamo Biosciences, Inc. Methods and compositions for modulating PD1
JP5763341B2 (en) * 2007-09-27 2015-08-12 サンガモ バイオサイエンシーズ， インコーポレイテッド Rapid in vivo identification of biologically active nucleases
EP2597155B1 (en) 2007-10-25 2016-11-23 Sangamo BioSciences, Inc. Methods and compositions for targeted integration
WO2009076292A2 (en) * 2007-12-07 2009-06-18 Precision Biosciences, Inc. Rationally-designed meganucleases with recognition sequences found in dnase hypersensitive regions of the human genome
WO2009114321A2 (en) * 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
US20100071083A1 (en) * 2008-03-12 2010-03-18 Smith James J Temperature-dependent meganuclease activity
CA2720903C (en) 2008-04-14 2019-01-15 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
WO2009146179A1 (en) * 2008-04-15 2009-12-03 University Of Iowa Research Foundation Zinc finger nuclease for the cftr gene and methods of use thereof
JP2011519558A (en) * 2008-04-28 2011-07-14 プレシジョン バイオサイエンシズ，インク． A rationally designed fusion molecule of DNA-binding protein and effector domain
JP2011527906A (en) 2008-07-14 2011-11-10 プレシジョン バイオサイエンシズ，インク． Recognition sequences for I-CreI derived meganucleases and uses thereof
JP5908725B2 (en) 2008-08-22 2016-04-26 サンガモ バイオサイエンシーズ， インコーポレイテッド Methods and compositions for targeted single-strand cleavage and targeted integration
EP2180058A1 (en) * 2008-10-23 2010-04-28 Cellectis Meganuclease recombination system
JP5756016B2 (en) * 2008-10-29 2015-07-29 サンガモ バイオサイエンシーズ， インコーポレイテッド Method and composition for inactivating expression of glutamine synthetase gene
US20110023144A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved in amyotrophyic lateral sclerosis disease
US20110030072A1 (en) * 2008-12-04 2011-02-03 Sigma-Aldrich Co. Genome editing of immunodeficiency genes in animals
US20110016543A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Genomic editing of genes involved in inflammation
US20110023158A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Bovine genome editing with zinc finger nucleases
US20110023149A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved in tumor suppression in animals
US20110023159A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Ovine genome editing with zinc finger nucleases
US20110023150A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genome editing of genes associated with schizophrenia in animals
US20110023147A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of prion disorder-related genes in animals
US20110016540A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Genome editing of genes associated with trinucleotide repeat expansion disorders in animals
US20110023139A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved in cardiovascular disease
US20110016539A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Genome editing of neurotransmission-related genes in animals
US20110023146A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved in secretase-associated disorders
US20110016546A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Porcine genome editing with zinc finger nucleases
US20110016541A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Genome editing of sensory-related genes in animals
US20110016542A1 (en) * 2008-12-04 2011-01-20 Sigma-Aldrich Co. Canine genome editing with zinc finger nucleases
US20110023143A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of neurodevelopmental genes in animals
US20110023152A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genome editing of cognition related genes in animals
US20110023157A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Equine genome editing with zinc finger nucleases
US20110023154A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Silkworm genome editing with zinc finger nucleases
US20110023156A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Feline genome editing with zinc finger nucleases
US20110023141A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved with parkinson's disease
US20110023145A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved in autism spectrum disorders
US20110023148A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genome editing of addiction-related genes in animals
US20110023153A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genomic editing of genes involved in alzheimer's disease
US20110023140A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Rabbit genome editing with zinc finger nucleases
US20110023151A1 (en) * 2008-12-04 2011-01-27 Sigma-Aldrich Co. Genome editing of abc transporters
EP3156494B8 (en) 2008-12-04 2018-09-19 Sangamo Therapeutics, Inc. Genome editing in rats using zinc-finger nucleases
AU2009333863B2 (en) * 2008-12-17 2015-02-05 Corteva Agriscience Llc Targeted integration into the Zp15 locus
CA2755192C (en) * 2009-03-20 2018-09-11 Sangamo Biosciences, Inc. Modification of cxcr4 using engineered zinc finger proteins
AU2010235161B2 (en) 2009-04-09 2015-01-22 Sangamo Therapeutics, Inc. Targeted integration into stem cells
US8772008B2 (en) * 2009-05-18 2014-07-08 Sangamo Biosciences, Inc. Methods and compositions for increasing nuclease activity
EP2449135B1 (en) 2009-06-30 2016-01-06 Sangamo BioSciences, Inc. Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
WO2011005849A1 (en) 2009-07-08 2011-01-13 Cellular Dynamics International, Inc. Modified ips cells having a mutant form of human immunodeficiency virus (hiv) cellular entry gene
CN102858985A (en) * 2009-07-24 2013-01-02 西格马-奥尔德里奇有限责任公司 Genome editing method
KR20120097483A (en) * 2009-07-24 2012-09-04 시그마-알드리치 컴퍼니., 엘엘씨 Method for genome editing
CA2769262C (en) * 2009-07-28 2019-04-30 Sangamo Biosciences, Inc. Methods and compositions for treating trinucleotide repeat disorders
CN102612565B (en) * 2009-08-11 2016-04-06 桑格摩生物科学股份有限公司 Homozygous organisms for targeted modification
CA2779337A1 (en) * 2009-10-28 2011-05-05 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Fuer Gesundheit U Nd Umwelt (Gmbh) Homologous recombination in the oocyte
US8956828B2 (en) 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
WO2011078662A1 (en) * 2009-12-21 2011-06-30 Keygene N.V. Dsrna for improved genetic modification of plant dna
CA2787494C (en) 2010-01-22 2019-09-17 Dow Agrosciences Llc Targeted genomic alteration
EP2526199A4 (en) * 2010-01-22 2013-08-07 Scripps Research Inst METHODS FOR PRODUCING ZINC FINGER NUCLEASES HAVING MODIFIED ACTIVITY
CA2787674C (en) * 2010-01-22 2022-09-06 Dow Agrosciences Llc Excision of transgenes in genetically modified organisms
JP6137596B2 (en) 2010-02-08 2017-05-31 サンガモ セラピューティクス， インコーポレイテッド Engineered truncated half-domain
US9255259B2 (en) 2010-02-09 2016-02-09 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
US9567573B2 (en) 2010-04-26 2017-02-14 Sangamo Biosciences, Inc. Genome editing of a Rosa locus using nucleases
AU2011256838B2 (en) 2010-05-17 2014-10-09 Sangamo Therapeutics, Inc. Novel DNA-binding proteins and uses thereof
CA2805442C (en) 2010-07-21 2020-05-12 Sangamo Biosciences, Inc. Methods and compositions for modification of an hla locus
WO2012012738A1 (en) * 2010-07-23 2012-01-26 Sigma-Aldrich Co., Llc Genome editing using targeting endonucleases and single-stranded nucleic acids
WO2012018726A1 (en) * 2010-08-02 2012-02-09 Cellectis Sa Method for increasing double-strand break-induced gene targeting
EP3511420B1 (en) 2010-09-27 2021-04-07 Sangamo Therapeutics, Inc. Compositions for inhibiting viral entry into cells
EP2627665B1 (en) 2010-10-12 2015-12-16 The Children's Hospital Of Philadelphia Methods and compositions for treating hemophilia B
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
WO2012094132A1 (en) 2011-01-05 2012-07-12 Sangamo Biosciences, Inc. Methods and compositions for gene correction
CN102174649B (en) * 2011-01-18 2013-06-12 陕西师范大学 Method for rapidly detecting zinc-finger nuclease mediated gene fixed point integration
US9528124B2 (en) 2013-08-27 2016-12-27 Recombinetics, Inc. Efficient non-meiotic allele introgression
US10920242B2 (en) 2011-02-25 2021-02-16 Recombinetics, Inc. Non-meiotic allele introgression
CN102206631A (en) * 2011-03-08 2011-10-05 西北农林科技大学 Open-source method for screening zinc finger proteins targeted combined with target sites of human DYRK1A gene
CN102358902B (en) * 2011-04-02 2013-01-02 西南大学 Silkworm fibroin heavy-chain gene mutation sequence and mutation method and application
WO2012152912A1 (en) 2011-05-12 2012-11-15 Newvectys Genetically modified pig as a cancer prone model
KR20140066977A (en) 2011-06-08 2014-06-03 미에드지나로도위 인스티투트 바이오로지 모레쿠라네지 아이 코모르코웨즈 Sequence-specific engineered ribonuclease h and the method for determining the sequence preference of dna-rna hybrid binding proteins
CA2841541C (en) 2011-07-25 2019-11-12 Sangamo Biosciences, Inc. Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (cftr) gene
EP2758529B1 (en) 2011-09-21 2019-03-20 Sangamo Therapeutics, Inc. Methods and compositions for regulation of transgene expression
US9222105B2 (en) 2011-10-27 2015-12-29 Sangamo Biosciences, Inc. Methods and compositions for modification of the HPRT locus
WO2013074999A1 (en) 2011-11-16 2013-05-23 Sangamo Biosciences, Inc. Modified dna-binding proteins and uses thereof
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
CA2865011C (en) 2012-02-29 2021-06-15 Sangamo Biosciences, Inc. Methods and compositions for treating huntington's disease
ES2683071T3 (en) 2012-04-25 2018-09-24 Regeneron Pharmaceuticals, Inc. Nuclease-mediated addressing with large addressing vectors
AU2013256240B2 (en) * 2012-05-02 2018-09-20 Corteva Agriscience Llc Targeted modification of malate dehydrogenase
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
ES2728782T3 (en) 2012-05-25 2019-10-28 Univ California Methods and compositions for RNA-directed target DNA modification and for RNA-directed transcription modulation
JP2015525077A (en) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド Chip configuration and highly accurate nucleic acid sequencing
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
WO2014011901A2 (en) 2012-07-11 2014-01-16 Sangamo Biosciences, Inc. Methods and compositions for delivery of biologics
US10648001B2 (en) 2012-07-11 2020-05-12 Sangamo Therapeutics, Inc. Method of treating mucopolysaccharidosis type I or II
HUE051612T2 (en) 2012-07-11 2021-03-01 Sangamo Therapeutics Inc Methods and compositions for treating lysosomal storage diseases
US10058078B2 (en) 2012-07-31 2018-08-28 Recombinetics, Inc. Production of FMDV-resistant livestock by allele substitution
EP2890780B8 (en) 2012-08-29 2020-08-19 Sangamo Therapeutics, Inc. Methods and compositions for treatment of a genetic condition
UA118090C2 (en) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі METHOD OF THE METHER OF THE METHOD OF THE INTEGRED EMBLED SUBSTITUTION OF NUCLEIC NUCLE OF NUCLEIC ACID AND NON-NUCLIC ACID AND NON-SPECIAL SPECIES
BR112015004995B1 (en) 2012-09-07 2023-05-02 Sangamo Biosciences, Inc. METHOD FOR MODIFYING THE GENOME OF A CELL, USE OF A CELL, SEED OR PLANT OBTAINED BY THE SAID METHOD AND SITE-SPECIFIC ZINC FINGER NUCLEASE
UA119135C2 (en) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Engineered transgene integration platform (etip) for gene targeting and trait stacking
WO2014059173A2 (en) 2012-10-10 2014-04-17 Sangamo Biosciences, Inc. T cell modifying compounds and uses thereof
CN103215234B (en) * 2012-11-09 2014-12-10 陕西师范大学 Adenovirus with zinc finger nuclease expression element and donor DNA, and construction method and application
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
CA2892448A1 (en) * 2012-12-05 2014-06-12 Sangamo Biosciences, Inc. Methods and compositions for regulation of metabolic disorders
DK3363902T3 (en) * 2012-12-06 2020-01-02 Sigma Aldrich Co Llc CRISPR-BASED RE-MODIFICATION AND REGULATION
CA2895117A1 (en) * 2012-12-13 2014-06-19 James W. Bing Precision gene targeting to a particular locus in maize
EP2935319B1 (en) 2012-12-18 2019-04-24 Novartis AG Production of therapeutic proteins in genetically modified mammalian cells
EP3491915B1 (en) 2012-12-27 2023-05-31 Keygene N.V. Method for inducing a targeted translocation in a plant.
MX2015010841A (en) 2013-02-20 2016-05-09 Regeneron Pharma Genetic modification of rats.
US10227610B2 (en) 2013-02-25 2019-03-12 Sangamo Therapeutics, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
CN105189788B (en) 2013-03-12 2018-07-13 纳幕尔杜邦公司 Method of variant recognition site for identifying rare cutting engineering double-strand break derivant and combinations thereof and purposes
ES2749624T3 (en) 2013-03-14 2020-03-23 Caribou Biosciences Inc Nucleic Acid Targeted Nucleic Acid Compositions and Methods
ES2786253T3 (en) 2013-03-14 2020-10-09 Immusoft Corp Methods for the in vitro differentiation and transduction of memory b cells with pseudotyped viral vectors vsv-g
AU2014235968B2 (en) 2013-03-21 2018-05-24 Ospedale San Raffaele Srl Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
CN105263312A (en) 2013-04-05 2016-01-20 美国陶氏益农公司 Methods and compositions for integration of an exogenous sequence within the genome of plants
DK3456831T3 (en) 2013-04-16 2021-09-06 Regeneron Pharma TARGETED MODIFICATION OF RAT GENOMES
US10604771B2 (en) * 2013-05-10 2020-03-31 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
EP2997146A4 (en) 2013-05-15 2017-04-26 Sangamo BioSciences, Inc. Methods and compositions for treatment of a genetic condition
US20160145631A1 (en) * 2013-06-14 2016-05-26 Cellectis Methods for non-transgenic genome editing in plants
US11306328B2 (en) * 2013-07-26 2022-04-19 President And Fellows Of Harvard College Genome engineering
WO2015017866A1 (en) 2013-08-02 2015-02-05 Enevolv, Inc. Processes and host cells for genome, pathway, and biomolecular engineering
CA2920899C (en) 2013-08-28 2023-02-28 Sangamo Biosciences, Inc. Compositions for linking dna-binding domains and cleavage domains
BR102014021800B1 (en) 2013-09-04 2022-03-29 Dow Agrosciences Llc Method for detecting site-specific integration of a polynucleotide donor sequence within a genomic target site
US10117899B2 (en) 2013-10-17 2018-11-06 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering in hematopoietic stem cells
EP3058072B1 (en) 2013-10-17 2021-05-19 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
EP3060918B1 (en) 2013-10-23 2019-09-18 Genia Technologies, Inc. High speed molecular sensing with nanopores
JP6633532B2 (en) 2013-11-04 2020-01-22 ダウ アグロサイエンシィズ エルエルシー Optimal corn loci
WO2015066643A1 (en) 2013-11-04 2015-05-07 Dow Agrosciences Llc Optimal soybean loci
CN105980395A (en) 2013-11-04 2016-09-28 美国陶氏益农公司 Optimal soybean loci
EP3066110B1 (en) 2013-11-04 2021-12-29 Corteva Agriscience LLC Optimal maize loci
MX391904B (en) 2013-11-11 2025-03-12 Sangamo Biosciences Inc A GENETIC REPRESSOR FOR USE IN THE TREATMENT OF HUNTINGTON'S DISEASE.
ES2721936T3 (en) 2013-11-13 2019-08-06 Childrens Medical Center Regulation of nuclease-mediated gene expression
US9951353B2 (en) * 2013-11-15 2018-04-24 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Engineering neural stem cells using homologous recombination
CA3229275A1 (en) 2013-12-09 2015-06-18 Sangamo Biosciences, Inc. Methods and compositions for genome engineering
MX388127B (en) 2013-12-11 2025-03-19 Regeneron Pharma METHODS AND COMPOSITIONS FOR THE TARGETED MODIFICATION OF A GENOME.
EP3460063B1 (en) 2013-12-11 2024-03-13 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a genome
JP2017505756A (en) 2013-12-13 2017-02-23 ザ ジェネラル ホスピタル コーポレイション Soluble high molecular weight (HMW) tau species and uses thereof
US10774338B2 (en) 2014-01-16 2020-09-15 The Regents Of The University Of California Generation of heritable chimeric plant traits
PT3102673T (en) 2014-02-03 2020-05-21 Sangamo Biosciences Inc Methods and compositions for treatment of a beta thalessemia
US10036034B2 (en) 2014-02-13 2018-07-31 Nutech Ventures Sequences involved in plant yield and methods of using
JP6416939B2 (en) 2014-02-13 2018-10-31 タカラ バイオ ユーエスエー，インコーポレイティド Method for depleting target molecules from an initial collection of nucleic acids, and compositions and kits for performing the same
US10323251B2 (en) 2014-02-19 2019-06-18 National Research And Development Agency National Agriculture And Food Research Organization Method utilizing piggybac transposon and transposase to introduce mutation into target DNA in a plant cell
EP3110454B1 (en) 2014-02-24 2020-11-18 Sangamo Therapeutics, Inc. Methods and compositions for nuclease-mediated targeted integration
JP6521669B2 (en) * 2014-02-25 2019-05-29 国立研究開発法人農業・食品産業技術総合研究機構 Plant cell in which mutation is introduced into target DNA, and method for producing the same
EP3613854A1 (en) 2014-03-05 2020-02-26 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
EP3929279A1 (en) 2014-03-18 2021-12-29 Sangamo Therapeutics, Inc. Methods and compositions for regulation of zinc finger protein expression
US9522936B2 (en) 2014-04-24 2016-12-20 Sangamo Biosciences, Inc. Engineered transcription activator like effector (TALE) proteins
BR122023023211A2 (en) 2014-04-28 2024-01-23 Recombinetics, Inc. METHOD OF MAKING MULTIPLEX GENE EDITS IN A VERTEBRATE OR PRIMARY NON-HUMAN EMBRYO CELL
BR112016025849A2 (en) 2014-05-08 2017-10-17 Chdi Foundation Inc methods and compositions for the treatment of huntington's disease
WO2015175642A2 (en) 2014-05-13 2015-11-19 Sangamo Biosciences, Inc. Methods and compositions for prevention or treatment of a disease
US9970001B2 (en) 2014-06-05 2018-05-15 Sangamo Therapeutics, Inc. Methods and compositions for nuclease design
DK3152312T3 (en) 2014-06-06 2020-04-06 Regeneron Pharma METHODS AND COMPOSITIONS FOR MODIFICATION OF A TARGET LOCUS
CN106659290A (en) * 2014-06-19 2017-05-10 高露洁-棕榄公司 Oral Care Appliances
US9738897B2 (en) 2014-06-23 2017-08-22 Regeneron Pharmaceuticals, Inc. Nuclease-mediated DNA assembly
DK3161128T3 (en) 2014-06-26 2018-11-05 Regeneron Pharma METHODS AND COMPOSITIONS FOR TARGETED GENTICAL MODIFICATIONS AND PROCEDURES FOR USE THEREOF
US20170159065A1 (en) 2014-07-08 2017-06-08 Vib Vzw Means and methods to increase plant yield
WO2016014794A1 (en) 2014-07-25 2016-01-28 Sangamo Biosciences, Inc. Methods and compositions for modulating nuclease-mediated genome engineering in hematopoietic stem cells
WO2016014837A1 (en) 2014-07-25 2016-01-28 Sangamo Biosciences, Inc. Gene editing for hiv gene therapy
US9616090B2 (en) 2014-07-30 2017-04-11 Sangamo Biosciences, Inc. Gene correction of SCID-related genes in hematopoietic stem and progenitor cells
CN113699113A (en) 2014-09-16 2021-11-26 桑格摩治疗股份有限公司 Methods and compositions for nuclease-mediated genome engineering and correction in hematopoietic stem cells
AU2015320738B2 (en) 2014-09-23 2021-12-02 Acceligen, Inc. Materials and methods for producing animals with short hair
BR112017007770A2 (en) 2014-10-15 2018-01-16 Regeneron Pharma in vitro culture, hipscs population, method for modifying a genomic target locus, and, hipsc.
JP6153180B2 (en) 2014-11-04 2017-06-28 国立大学法人神戸大学 Method for modifying genomic sequence, which specifically introduces mutation into DNA sequence targeted by abasic reaction, and molecular complex used therefor
CA2967027A1 (en) 2014-11-12 2016-05-19 Recombinetics, Inc. Heterozygous modifications of tumor suppressor genes
RU2756247C2 (en) 2014-11-17 2021-09-28 Эдисет Био, Инк. Genetically modified gamma delta t cells
EP3221457B1 (en) 2014-11-21 2019-03-20 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide rnas
BR112017010793A2 (en) 2014-11-24 2017-12-26 Regeneron Pharma genetically modified nonhuman animal, mouse, methods for producing a genetically modified nonhuman animal and screening for drug candidates directed to an antigen of interest, and, mouse model.
CN104450785A (en) * 2014-12-08 2015-03-25 复旦大学 Genome editing method using attachment carrier for encoding targeted endonuclease and kit
US10889834B2 (en) 2014-12-15 2021-01-12 Sangamo Therapeutics, Inc. Methods and compositions for enhancing targeted transgene integration
MX388784B (en) 2014-12-19 2025-03-20 Regeneron Pharma METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION THROUGH SINGLE-STEP MULTIPLE TARGETING.
US20180002379A1 (en) 2015-01-21 2018-01-04 Sangamo Therapeutics, Inc. Methods and compositions for identification of highly specific nucleases
CA2981077A1 (en) 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b-cells
WO2016176617A2 (en) 2015-04-29 2016-11-03 New York University Method for treating high-grade gliomas
US10179918B2 (en) 2015-05-07 2019-01-15 Sangamo Therapeutics, Inc. Methods and compositions for increasing transgene activity
SG10202112057QA (en) 2015-05-12 2021-12-30 Sangamo Therapeutics Inc Nuclease-mediated regulation of gene expression
US9957501B2 (en) 2015-06-18 2018-05-01 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
EP3320773B9 (en) 2015-07-10 2023-10-11 Chugai Seiyaku Kabushiki Kaisha Mouse having human cd3 gene substituted for endogenous cd3 gene
CA2991301A1 (en) 2015-07-13 2017-01-19 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
JP6664693B2 (en) 2015-09-09 2020-03-13 国立大学法人神戸大学 Method for converting genomic sequence of gram-positive bacteria, specifically converting nucleobase of targeted DNA sequence, and molecular complex used therein
DK3352776T3 (en) 2015-09-23 2025-06-23 Sangamo Therapeutics Inc HTT REPRESSORS AND THEIR APPLICATION
EP3673732A3 (en) 2015-10-27 2020-07-29 Recombinetics, Inc. Engineering of humanized car t-cells and platelets by genetic complementation
US10143760B2 (en) 2015-10-28 2018-12-04 Sangamo Therapeutics, Inc. Liver-specific constructs, factor VIII expression cassettes and methods of use thereof
EP3368673B1 (en) 2015-10-29 2020-07-29 Amyris, Inc. Compositions and methods for production of myrcene
WO2017079428A1 (en) 2015-11-04 2017-05-11 President And Fellows Of Harvard College Site specific germline modification
EP3380622A4 (en) 2015-11-23 2019-08-07 Sangamo Therapeutics, Inc. Methods and compositions for engineering immunity
CN108495932B (en) 2015-11-27 2022-08-09 国立大学法人神户大学 Method for converting genomic sequence of monocotyledon for specifically converting nucleobase targeting DNA sequence, and molecular complex used therefor
US11352631B2 (en) 2015-12-18 2022-06-07 Sangamo Therapeutics, Inc. Targeted disruption of the T cell receptor
AU2016374253B2 (en) 2015-12-18 2021-10-21 Sangamo Therapeutics, Inc. Targeted disruption of the MHC cell receptor
EP3402533B1 (en) 2016-01-15 2021-08-04 Sangamo Therapeutics, Inc. Methods and compositions for the treatment of neurologic disease
JP7012650B2 (en) 2016-02-02 2022-01-28 サンガモ セラピューティクス， インコーポレイテッド Composition for linking DNA binding domain and cleavage domain
ES2919961T3 (en) 2016-04-21 2022-07-29 Univ Kobe Nat Univ Corp Method to increase the efficiency of introducing mutations in a genomic sequence modification technique, and molecular complex for its use in the same
CN118421557A (en) 2016-05-12 2024-08-02 阿迪塞特治疗公司 Methods and compositions for selectively expanding γδT cell populations
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
WO2018013840A1 (en) 2016-07-13 2018-01-18 Vertex Pharmaceuticals Incorporated Methods, compositions and kits for increasing genome editing efficiency
CN109803530A (en) 2016-07-29 2019-05-24 瑞泽恩制药公司 Mouse comprising causing the mutation of the truncated old information model expression of C-
WO2018029034A1 (en) 2016-08-09 2018-02-15 Vib Vzw Cellulose synthase inhibitors and mutant plants
US11774442B2 (en) 2016-08-15 2023-10-03 Enevolv, Inc. Molecule sensor systems
WO2018038046A1 (en) * 2016-08-22 2018-03-01 中外製薬株式会社 Gene-modified non-human animal expressing human gpc3 polypeptide
IL264792B2 (en) 2016-08-24 2023-10-01 Sangamo Therapeutics Inc Engineered target-specific zinc finger nucleases
WO2018039440A1 (en) 2016-08-24 2018-03-01 Sangamo Therapeutics, Inc. Regulation of gene expression using engineered nucleases
JP7256739B2 (en) 2016-09-07 2023-04-12 サンガモ セラピューティクス， インコーポレイテッド Modulation of liver genes
US20180084767A1 (en) 2016-09-21 2018-03-29 Recombinetics, Inc. Animal models for cardiomyopathy
DK3757120T3 (en) 2016-10-04 2022-07-25 Prec Biosciences Inc CO-STIMULATORY DOMAINS FOR USE IN GENETICALLY MODIFIED CELLS
GB201617559D0 (en) 2016-10-17 2016-11-30 University Court Of The University Of Edinburgh The Swine comprising modified cd163 and associated methods
KR102712926B1 (en) 2016-10-20 2024-10-07 상가모 테라퓨틱스, 인코포레이티드 Methods and compositions for the treatment of Fabry disease
WO2018081775A1 (en) 2016-10-31 2018-05-03 Sangamo Therapeutics, Inc. Gene correction of scid-related genes in hematopoietic stem and progenitor cells
CA3043019A1 (en) * 2016-11-21 2018-05-24 Dow Agrosciences Llc Site specific integration of a transgene using intra-genomic recombination via a non-homologous end joining repair pathway
EP4276187A3 (en) 2016-12-08 2024-01-17 Case Western Reserve University Methods and compositions for enhancing functional myelin production
CA3050715A1 (en) 2017-01-19 2018-07-26 Open Monoclonal Technology, Inc. Human antibodies from transgenic rodents with multiple heavy chain immunoglobulin loci
CA3049980A1 (en) 2017-01-23 2018-07-26 Regeneron Pharmaceuticals, Inc. Hydroxysteroid 17-beta dehydrogenase 13 (hsd17b13) variants and uses thereof
WO2018174097A1 (en) 2017-03-22 2018-09-27 国立大学法人神戸大学 Method for converting nucleic acid sequence of cell specifically converting nucleic acid base of targeted dna using cell endogenous dna modifying enzyme, and molecular complex used therein
US11820728B2 (en) 2017-04-28 2023-11-21 Acuitas Therapeutics, Inc. Carbonyl lipids and lipid nanoparticle formulations for delivery of nucleic acids
JP2020518245A (en) 2017-05-03 2020-06-25 サンガモ セラピューティクス， インコーポレイテッド Methods and compositions for modification of the cystic fibrosis transmembrane conductance regulator (CFTR) gene
JP7170666B2 (en) 2017-05-08 2022-11-14 プレシジョン バイオサイエンシズ，インク． Nucleic Acid Molecules Encoding Engineered Antigen Receptors and Inhibitory Nucleic Acid Molecules, and Methods of Their Use
EP3409104A1 (en) 2017-05-31 2018-12-05 Vilmorin et Cie Tomato plant resistant to tomato yellow leaf curl virus, powdery mildew, and nematodes
WO2020249996A1 (en) 2019-06-14 2020-12-17 Vilmorin & Cie Resistance in plants of solanum lycopersicum to the tobamovirus tomato brown rugose fruit virus
EP3409106A1 (en) 2017-06-01 2018-12-05 Vilmorin et Cie Tolerance in plants of solanum lycopersicum to the tobamovirus tomato brown rugose fruit virus (tbrfv)
DK3635102T3 (en) 2017-06-05 2025-10-27 Regeneron Pharma B4GALT1 VARIANTS AND THEIR USES
US11512287B2 (en) 2017-06-16 2022-11-29 Sangamo Therapeutics, Inc. Targeted disruption of T cell and/or HLA receptors
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
MX2019015505A (en) 2017-06-30 2020-07-28 Inscripta Inc Automated cell processing methods, modules, instruments, and systems.
EP4775669A2 (en) 2017-06-30 2026-07-15 Precision Biosciences, Inc. Genetically-modified t cells comprising a modified intron in the t cell receptor alpha gene
WO2019006464A1 (en) 2017-06-30 2019-01-03 Memorial Sloan Kettering Cancer Center Compositions and methods for adoptive cell therapy for cancer
SG10201913147WA (en) 2017-07-11 2020-02-27 Compass Therapeutics Llc Agonist antibodies that bind human cd137 and uses thereof
CN111182790A (en) 2017-07-31 2020-05-19 瑞泽恩制药公司 CRISPR reporter non-human animals and uses thereof
KR102712142B1 (en) 2017-07-31 2024-10-07 리제너론 파마슈티칼스 인코포레이티드 Cas-transgenic mouse embryonic stem cells and mice and uses thereof
CA3065579A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Assessment of crispr/cas-induced recombination with an exogenous donor nucleic acid in vivo
JP2020530997A (en) 2017-08-08 2020-11-05 サンガモ セラピューティクス， インコーポレイテッド Chimeric antigen receptor-mediated cell targeting
US20190045761A1 (en) 2017-08-11 2019-02-14 Recombinetics, Inc. Inducible disease models methods of making them and use in tissue complementation
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
HUE073358T2 (en) 2017-08-29 2026-01-28 Kws Saat Se & Co Kgaa Improved blue aleurone and other segregation systems
EP3684389A4 (en) 2017-09-21 2021-06-16 Dana Farber Cancer Institute, Inc. ISOLATION, STORAGE, COMPOSITIONS AND USES OF JUSTICIA PLANT EXTRACTS
WO2019067875A1 (en) 2017-09-29 2019-04-04 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus and methods of use
US10435713B2 (en) 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation
EP3692057B9 (en) 2017-10-03 2023-10-04 Precision BioSciences, Inc. Modified epidermal growth factor receptor peptides for use in genetically-modified cells
US11718679B2 (en) 2017-10-31 2023-08-08 Compass Therapeutics Llc CD137 antibodies and PD-1 antagonists and uses thereof
EP3704238B1 (en) 2017-11-01 2024-01-03 Precision Biosciences, Inc. Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a
WO2019094725A2 (en) 2017-11-09 2019-05-16 Sangamo Therapeutics, Inc. Genetic modification of cytokine inducible sh2-containing protein (cish) gene
CA3082818A1 (en) 2017-11-15 2019-05-23 Adicet Bio Inc. Methods for selective expansion of .delta.3 .gamma..delta. t-cell populations and compositions thereof
EP3713961A2 (en) 2017-11-20 2020-09-30 Compass Therapeutics LLC Cd137 antibodies and tumor antigen-targeting antibodies and uses thereof
EP3715454A4 (en) 2017-11-22 2021-08-18 National University Corporation Kobe University COMPLEX FOR SAFE GENE EDITION WITH LOW SIDE EFFECTS, AND NUCLEIC ACID CODING THIS
BR112020014140A2 (en) 2018-01-17 2020-12-01 Vertex Pharmaceuticals Incorporated dna-pk inhibitors
US12269804B2 (en) 2018-01-17 2025-04-08 Vertex Pharmaceuticals Incorporated Quinoxalinone compounds, compositions, methods, and kits for increasing genome editing efficiency
WO2019143678A1 (en) 2018-01-17 2019-07-25 Vertex Pharmaceuticals Incorporated Dna-pk inhibitors
BR112020015488A2 (en) 2018-02-08 2020-12-08 Sangamo Therapeutics, Inc. MODIFIED TARGET-SPECIFIC NUCLEASES
CN112041432A (en) 2018-02-15 2020-12-04 纪念斯隆-凯特林癌症中心 FOXP3 targeting agent compositions and methods of use for adoptive cell therapy
CA3093716A1 (en) 2018-03-16 2019-09-19 Immusoft Corporation B cells genetically engineered to secrete follistatin and methods of using the same to treat follistatin-related diseases, conditions, disorders and to enhance muscle growth and strength
EP3592140A1 (en) 2018-03-19 2020-01-15 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems
EP3660152A4 (en) 2018-03-26 2020-07-15 National University Corporation Kobe University METHOD FOR MODIFYING THE DESTINATION IN DOUBLE-STRANDED DNA IN A CELL
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
CN112585276A (en) 2018-04-05 2021-03-30 朱诺治疗学股份有限公司 Methods of producing cells expressing recombinant receptors and related compositions
CN112585277A (en) 2018-04-05 2021-03-30 朱诺治疗学股份有限公司 T cells expressing recombinant receptors, related polynucleotides and methods
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
JP7332622B2 (en) 2018-04-18 2023-08-23 サンガモ セラピューティクス， インコーポレイテッド Zinc finger protein compositions for regulation of huntingtin (HTT)
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10508273B2 (en) 2018-04-24 2019-12-17 Inscripta, Inc. Methods for identifying selective binding pairs
US11690921B2 (en) 2018-05-18 2023-07-04 Sangamo Therapeutics, Inc. Delivery of target specific nucleases
GB201809273D0 (en) 2018-06-06 2018-07-25 Vib Vzw Novel mutant plant cinnamoyl-coa reductase proteins
JP7367974B2 (en) 2018-07-31 2023-10-24 国立大学法人 東京大学 Membrane protein activity measurement method
AU2019317066A1 (en) 2018-08-07 2021-02-18 Modalis Therapeutics Corporation Novel transcription activator
CN109097370A (en) * 2018-08-08 2018-12-28 华南农业大学 A method of regulation tea tree fine hair formation
EP3607819A1 (en) 2018-08-10 2020-02-12 Vilmorin et Cie Resistance to xanthomonas campestris pv. campestris (xcc) in cauliflower
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
JP7541508B2 (en) 2018-08-23 2024-08-28 サンガモ セラピューティクス， インコーポレイテッド Engineered target-specific base editors
US11708569B2 (en) 2018-08-29 2023-07-25 University Of Copenhagen Modified recombinant lysosomal alpha-galactosidase A and aspartylglucoaminidase having low mannose-6-phosphate and high sialic acid
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
EP4234570A3 (en) 2018-09-18 2023-12-13 Sangamo Therapeutics, Inc. Programmed cell death 1 (pd1) specific nucleases
EP3852911B1 (en) 2018-09-21 2025-01-22 Acuitas Therapeutics, Inc. Systems and methods for manufacturing lipid nanoparticles and liposomes
IL326835A (en) 2018-10-16 2026-04-01 Blueallele Llc Methods for directing the insertion of DNA into genes
CA3116576A1 (en) 2018-10-18 2020-04-23 Acuitas Therapeutics, Inc. Lipids for lipid nanoparticle delivery of active agents
WO2020086475A1 (en) 2018-10-22 2020-04-30 Inscripta, Inc. Engineered enzymes
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
JP7497351B2 (en) 2018-11-13 2024-06-10 コンパス セラピューティクス リミテッド ライアビリティ カンパニー Multispecific binding constructs for checkpoint molecules and uses thereof
KR20210094609A (en) 2018-11-28 2021-07-29 포티 세븐, 인코포레이티드 Genetically Modified HSPCs Resistant to Ablation Regimes
CA3126896A1 (en) 2018-12-03 2020-06-11 Adicet Bio Inc. Methods for selective in vivo expansion of .gamma. .delta. t-cell populations and compositions thereof
KR20200071198A (en) 2018-12-10 2020-06-19 네오이뮨텍, 인코퍼레이티드 Development of new adoptive T cell immunotherapy by modification of Nrf2 expression
GB201820109D0 (en) 2018-12-11 2019-01-23 Vib Vzw Plants with a lignin trait and udp-glycosyltransferase mutation
WO2020131632A1 (en) 2018-12-20 2020-06-25 Regeneron Pharmaceuticals, Inc. Nuclease-mediated repeat expansion
EP3898661A1 (en) 2018-12-21 2021-10-27 Precision BioSciences, Inc. Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria
CN113423419A (en) 2019-01-04 2021-09-21 桑格摩生物治疗股份有限公司 Methods and compositions for treating fabry disease
PL3908568T3 (en) 2019-01-11 2024-12-02 Acuitas Therapeutics, Inc. Lipids for lipid nanoparticle delivery of active agents
CA3106693A1 (en) 2019-02-06 2020-08-13 Sangamo Therapeutics, Inc. Method for the treatment of mucopolysaccharidosis type i
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
WO2020205838A1 (en) 2019-04-02 2020-10-08 Sangamo Therapeutics, Inc. Methods for the treatment of beta-thalassemia
EP3945800A1 (en) 2019-04-03 2022-02-09 Regeneron Pharmaceuticals, Inc. Methods and compositions for insertion of antibody coding sequences into a safe harbor locus
EP4332115A3 (en) 2019-04-03 2024-05-29 Precision Biosciences, Inc. Genetically-modified immune cells comprising a microrna-adapted shrna (shrnamir)
AU2020253531C1 (en) 2019-04-04 2022-06-16 Regeneron Pharmaceuticals, Inc. Methods for scarless introduction of targeted modifications into targeting vectors
JP7506686B2 (en) 2019-04-04 2024-06-26 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Non-human animals containing a humanized coagulation factor 12 gene locus
WO2020204159A1 (en) 2019-04-05 2020-10-08 国立大学法人大阪大学 Method for producing knock-in cell
EP3947646A1 (en) 2019-04-05 2022-02-09 Precision BioSciences, Inc. Methods of preparing populations of genetically-modified immune cells
SG11202111372VA (en) 2019-05-01 2021-11-29 Juno Therapeutics Inc Cells expressing a chimeric receptor from a modified cd247 locus, related polynucleotides and methods
CA3136737A1 (en) 2019-05-01 2020-11-05 Juno Therapeutics, Inc. Cells expressing a recombinant receptor from a modified tgfbr2 locus, related polynucleotides and methods
EP3969466A4 (en) 2019-05-14 2023-01-18 Yield10 Bioscience, Inc. MODIFIED PLANTS COMPRISING AN UNRELATED PROMOTER POLYNUCLEOTIDE OPERATIONALLY LINKED TO A CODING SEQUENCE CODING FOR A TRANSCRIPTION FACTOR
WO2020247452A1 (en) 2019-06-04 2020-12-10 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus with a beta-slip mutation and methods of use
EP3953477A4 (en) 2019-06-06 2022-06-22 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
CN113939595A (en) 2019-06-07 2022-01-14 瑞泽恩制药公司 Non-human animals including humanized albumin loci
US20220259617A1 (en) 2019-06-13 2022-08-18 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
KR20220024053A (en) 2019-06-14 2022-03-03 리제너론 파마슈티칼스 인코포레이티드 model of tauopathy
CN114008070A (en) 2019-06-21 2022-02-01 因思科瑞普特公司 Genome-wide rationally engineered mutations leading to increased lysine production in Escherichia coli
WO2020254850A1 (en) 2019-06-21 2020-12-24 Vilmorin & Cie Improvement of quality and permanence of green color of peppers at maturity and over-maturity
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
WO2021011936A2 (en) 2019-07-18 2021-01-21 University Of Rochester Cell-type selective immunoprotection of cells
US20220273715A1 (en) 2019-07-25 2022-09-01 Precision Biosciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
WO2021019272A1 (en) 2019-07-31 2021-02-04 Vilmorin & Cie Tolerance to tolcndv in cucumber
WO2021035054A1 (en) 2019-08-20 2021-02-25 Precision Biosciences, Inc. Lymphodepletion dosing regimens for cellular immunotherapies
WO2021035170A1 (en) 2019-08-21 2021-02-25 Precision Biosciences, Inc. Compositions and methods for tcr reprogramming using fusion proteins
US20220411479A1 (en) 2019-10-30 2022-12-29 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
US12521451B2 (en) 2019-11-08 2026-01-13 Regeneron Pharmaceuticals, Inc. CRISPR and AAV strategies for x-linked juvenile retinoschisis therapy
EP4057802A1 (en) 2019-11-14 2022-09-21 Vilmorin & Cie Resistance to acidovorax valerianellae in corn salad
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
EP4069285A1 (en) 2019-12-06 2022-10-12 Precision BioSciences, Inc. Methods for cancer immunotherapy, using lymphodepletion regimens and cd19, cd20 or bcma allogeneic car t cells
AU2020402526B2 (en) 2019-12-10 2025-01-23 Inscripta, Inc. Novel MAD nucleases
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
WO2021126886A1 (en) 2019-12-18 2021-06-24 Inscripta, Inc. Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
CN115135370A (en) 2020-01-27 2022-09-30 因思科瑞普特公司 Electroporation module and instrument
WO2021158915A1 (en) 2020-02-06 2021-08-12 Precision Biosciences, Inc. Recombinant adeno-associated virus compositions and methods for producing and using the same
JP7837276B2 (en) 2020-03-04 2026-03-30 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Methods and compositions for sensitizing tumor cells to immunotherapy
US20230102342A1 (en) 2020-03-23 2023-03-30 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
US20230263121A1 (en) 2020-03-31 2023-08-24 Elo Life Systems Modulation of endogenous mogroside pathway genes in watermelon and other cucurbits
WO2021224395A1 (en) 2020-05-06 2021-11-11 Cellectis S.A. Methods for targeted insertion of exogenous sequences in cellular genomes
JP7891314B2 (en) 2020-05-06 2026-07-16 セレクティス ソシエテ アノニム Methods for genetically modifying cells to deliver therapeutic proteins
WO2021231259A1 (en) 2020-05-11 2021-11-18 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
KR20230022868A (en) 2020-05-13 2023-02-16 주노 쎄러퓨티크스 인코퍼레이티드 Method for producing donor-batch cells expressing a recombinant acceptor
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2021245435A1 (en) 2020-06-03 2021-12-09 Vilmorin & Cie Melon plants resistant to scab disease, aphids and powdery mildew
BR112022024738A2 (en) 2020-06-05 2023-03-07 Vilmorin & Cie PLANT, PLANT CELL, TISSUE CULTURE OF CELLS, PLANT PART, SEED, USE OF A PLANT, METHODS FOR DETECTING AND/OR SELECTING PLANTS, CONFERRING TOBRFV RESISTANCE, IMPROVING TOMATO YIELD AND REDUCING TOMATO PRODUCTION LOSS AND USES OF A MARKER
EP4171616A1 (en) 2020-06-26 2023-05-03 Juno Therapeutics GmbH Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
KR20230051172A (en) 2020-07-16 2023-04-17 아퀴타스 테라퓨틱스 인크. Cationic Lipids for Use in Lipid Nanoparticles
BR112023001272A2 (en) 2020-07-24 2023-04-04 Massachusetts Gen Hospital IMPROVED VIRUS-LIKE PARTICLES AND METHODS OF USING THEM FOR DELIVERY TO CELLS
EP4192875A1 (en) 2020-08-10 2023-06-14 Precision BioSciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
KR20230061474A (en) 2020-09-04 2023-05-08 고쿠리츠다이가쿠호진 고베다이가쿠 Double-stranded DNA modification complex containing miniaturized cytidine deaminase
EP4214314A4 (en) 2020-09-15 2024-10-16 Inscripta, Inc. CRISPR EDITING TO INCORPORATE NUCLEIC ACID DOCKING PLATES INTO LIVING CELL GENOMES
EP4221492A1 (en) 2020-10-02 2023-08-09 Vilmorin & Cie Melon with extended shelf life
US20230365995A1 (en) 2020-10-07 2023-11-16 Precision Biosciences, Inc. Lipid nanoparticle compositions
MX2023004761A (en) 2020-10-23 2023-07-17 Elo Life Systems Inc Methods for producing vanilla plants with improved flavor and agronomic production.
JP2023549780A (en) 2020-11-04 2023-11-29 ジュノー セラピューティクス インコーポレイテッド Cells expressing chimeric receptors from engineered invariant CD3 immunoglobulin superfamily chain loci and related polynucleotides and methods
US12637667B2 (en) 2020-11-06 2026-05-26 Editforce, Inc. FokI nuclease domain mutant
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
US12642203B2 (en) 2020-12-03 2026-06-02 Vilmorin & Cie Tomato plants resistant to ToBRFV, TMV, ToMV and ToMMV and corresponding resistance genes
CA3204158A1 (en) 2021-01-04 2022-07-07 Juhan Kim Mad nucleases
WO2022150269A1 (en) 2021-01-07 2022-07-14 Inscripta, Inc. Mad nucleases
WO2022165111A1 (en) 2021-01-28 2022-08-04 Precision Biosciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
CN118019546A (en) 2021-03-23 2024-05-10 艾欧凡斯生物治疗公司 CISH gene editing of tumor-infiltrating lymphocytes and its use in immunotherapy
WO2022208489A1 (en) 2021-04-02 2022-10-06 Vilmorin & Cie Semi-determinate or determinate growth habit trait in cucurbita
WO2022226316A1 (en) 2021-04-22 2022-10-27 Precision Biosciences, Inc. Compositions and methods for generating male sterile plants
IL282597B (en) * 2021-04-22 2022-08-01 B G Negev Technologies And Applications Ltd At Ben Gurion Univ Birds for creating female offspring and methods for creating them
EP4337769A1 (en) 2021-05-10 2024-03-20 SQZ Biotechnologies Company Methods for delivering genome editing molecules to the nucleus or cytosol of a cell and uses thereof
KR20240028362A (en) 2021-05-28 2024-03-05 라이엘 이뮤노파마, 인크. NR4A3-deficient immune cells and uses thereof
KR20240027676A (en) 2021-06-02 2024-03-04 라이엘 이뮤노파마, 인크. NR4A3-deficient immune cells and uses thereof
US20250280784A1 (en) 2021-08-06 2025-09-11 Vilmorin & Cie Resistance to leveillula taurica in pepper
WO2023035011A1 (en) 2021-09-03 2023-03-09 North Carolina State University Compositions and methods for conferring resistance to geminivirus
JP7125727B1 (en) 2021-09-07 2022-08-25 国立大学法人千葉大学 Compositions for modifying nucleic acid sequences and methods for modifying target sites in nucleic acid sequences
KR20240055084A (en) 2021-09-13 2024-04-26 라이프 테크놀로지스 코포레이션 gene editing tools
WO2023064872A1 (en) 2021-10-14 2023-04-20 Precision Biosciences, Inc. Combinations of anti-bcma car t cells and gamma secretase inhibitors
CN118119702A (en) 2021-10-14 2024-05-31 隆萨销售股份有限公司 Modified producer cells for extracellular vesicle production
MX2024004812A (en) 2021-10-19 2024-07-15 Prec Biosciences Inc Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency.
EP4422392A1 (en) 2021-10-25 2024-09-04 Fios Therapeutics, LLC Viable galactosyltransferase knock-out sheep and related methods
US12275947B2 (en) 2021-10-25 2025-04-15 Fios Therapeutics, Llc Viable genetically modified sheep with an inactivated alpha-1,3-galactosyltransferase (GGTA1) gene
KR20240099358A (en) 2021-10-27 2024-06-28 리제너론 파마슈티칼스 인코포레이티드 Compositions and methods for expressing factor IX for the treatment of hemophilia B
US20250043269A1 (en) 2021-11-03 2025-02-06 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glad Precise Genome Editing Using Retrons
WO2023081900A1 (en) 2021-11-08 2023-05-11 Juno Therapeutics, Inc. Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods
US20250129067A1 (en) 2021-11-08 2025-04-24 Progentos Therapeutics, Inc. Platelet-derived growth factor receptor (pdgfr) alpha inhibitors and uses thereof
US20250009800A1 (en) 2021-11-16 2025-01-09 Precision Biosciences, Inc. Methods for cancer immunotherapy
GB202117314D0 (en) 2021-11-30 2022-01-12 Clarke David John Cyclic nucleic acid fragmentation
AU2022408167A1 (en) 2021-12-08 2024-06-06 Regeneron Pharmaceuticals, Inc. Mutant myocilin disease model and uses thereof
GB202118058D0 (en) 2021-12-14 2022-01-26 Univ Warwick Methods to increase yields in crops
WO2023114943A2 (en) 2021-12-16 2023-06-22 Acuitas Therapeutics, Inc. Lipids for use in lipid nanoparticle formulations
KR20240128067A (en) 2021-12-29 2024-08-23 브리스톨-마이어스 스큅 컴퍼니 Generation of landing pad cell lines
WO2023131616A1 (en) 2022-01-05 2023-07-13 Vib Vzw Means and methods to increase abiotic stress tolerance in plants
WO2023131637A1 (en) 2022-01-06 2023-07-13 Vib Vzw Improved silage grasses
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2023144199A1 (en) 2022-01-26 2023-08-03 Vib Vzw Plants having reduced levels of bitter taste metabolites
IL314482A (en) 2022-02-02 2024-09-01 Regeneron Pharma Anti-TFR:GAA and anti-CD63:GAA insertions for the treatment of Pompe disease
WO2023150798A1 (en) 2022-02-07 2023-08-10 Regeneron Pharmaceuticals, Inc. Compositions and methods for defining optimal treatment timeframes in lysosomal disease
CN118613498A (en) 2022-02-11 2024-09-06 瑞泽恩制药公司 Compositions and methods for screening 4R Tau targeting agents
KR20240161175A (en) 2022-03-18 2024-11-12 옵시디안 테라퓨틱스, 인크. Small drug-responsive domains for modulating function/abundance and delivery of polypeptide payloads
EP4256950A1 (en) 2022-04-06 2023-10-11 Vilmorin et Cie Tolerance to cgmmv in cucumber
AU2023269134A1 (en) 2022-05-09 2024-12-12 Regeneron Pharmaceuticals, Inc. Vectors and methods for in vivo antibody production
US20260083846A1 (en) 2022-05-19 2026-03-26 Lyell Immunopharma, Inc. Polynucleotides targeting nr4a3 and uses thereof
GB2621813A (en) 2022-06-30 2024-02-28 Univ Newcastle Preventing disease recurrence in Mitochondrial replacement therapy
CN120659627A (en) 2022-07-29 2025-09-16 瑞泽恩制药公司 Compositions and methods for transferrin receptor (TFR) -mediated brain and muscle delivery
EP4565603A1 (en) 2022-08-05 2025-06-11 Regeneron Pharmaceuticals, Inc. Aggregation-resistant variants of tdp-43
AU2023329392A1 (en) 2022-08-25 2025-02-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024064958A1 (en) 2022-09-23 2024-03-28 Lyell Immunopharma, Inc. Methods for culturing nr4a-deficient cells
WO2024064952A1 (en) 2022-09-23 2024-03-28 Lyell Immunopharma, Inc. Methods for culturing nr4a-deficient cells overexpressing c-jun
KR20250075694A (en) 2022-09-28 2025-05-28 리제너론 파마슈티칼스 인코포레이티드 Antibody-resistant variant receptors to enhance cell-based therapies
WO2024077174A1 (en) 2022-10-05 2024-04-11 Lyell Immunopharma, Inc. Methods for culturing nr4a-deficient cells
AU2023371614A1 (en) 2022-11-04 2025-05-15 Regeneron Pharmaceuticals, Inc. Calcium voltage-gated channel auxiliary subunit gamma 1 (cacng1) binding proteins and cacng1-mediated delivery to skeletal muscle
WO2024100604A1 (en) 2022-11-09 2024-05-16 Juno Therapeutics Gmbh Methods for manufacturing engineered immune cells
AU2023379457A1 (en) 2022-11-14 2025-05-15 Regeneron Pharmaceuticals, Inc. Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes
CN120417757A (en) 2022-12-29 2025-08-01 维尔莫林公司 Tomato plants resistant to breakthrough-resistant TSWV strains and corresponding resistance genes
US20260232729A1 (en) 2023-02-03 2026-08-13 C3S2 Gmbh Methods for non-viral manufacturing of engineered immune cells
US12225874B2 (en) 2023-03-27 2025-02-18 Redsea Science And Technology Inc. Tomato plant designated ‘X22-31’
CN121311497A (en) 2023-04-14 2026-01-09 精密生物科学公司 Muscle-specific expression cassette
WO2024216116A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
AU2024261681A1 (en) 2023-04-28 2025-11-13 Vilmorin & Cie Genetic determinants conferring improved tobrfv resistance
WO2025003305A1 (en) 2023-06-28 2025-01-02 Vilmorin & Cie Pepper resistance to meloidogyne enterolobii
IL325779A (en) 2023-07-24 2026-03-01 Vilmorin & Cie Resistance to CYSDV in melon plants
AU2024315073A1 (en) 2023-07-28 2026-01-22 Regeneron Pharmaceuticals, Inc. Use of bgh-sv40l tandem polya to enhance transgene expression during unidirectional gene insertion
AR133384A1 (en) 2023-07-28 2025-09-24 Regeneron Pharma ANTI-TFR:ACID SPHINGOMYELINASE FOR THE TREATMENT OF ACID SPHINGOMYELINASE DEFICIENCY
IL326018A (en) 2023-07-28 2026-03-01 Regeneron Pharma Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease
EP4771049A1 (en) 2023-08-28 2026-07-08 Regeneron Pharmaceuticals, Inc. Cxcr4 antibody-resistant modified receptors
EP4771154A2 (en) 2023-09-01 2026-07-08 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
GB202314578D0 (en) 2023-09-22 2023-11-08 Univ Manchester Methods of producing homoplasmic modified plants or parts thereof
WO2025089333A1 (en) 2023-10-26 2025-05-01 公益財団法人京都大学ｉＰＳ細胞研究財団 Package of cell production device, cell production method, and management system therefor
WO2025135136A1 (en) 2023-12-20 2025-06-26 学校法人関西医科大学 Method for producing nail matrix cells
WO2025147573A2 (en) 2024-01-05 2025-07-10 Immusoft Corporation Glp-1 expressing modified b cells for the treatment of metabolic disease
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents
WO2025184567A1 (en) 2024-03-01 2025-09-04 Regeneron Pharmaceuticals, Inc. Methods and compositions for re-dosing aav using anti-cd40 antagonistic antibody to suppress host anti-aav antibody response
WO2025186284A1 (en) 2024-03-05 2025-09-12 Vilmorin & Cie Increased yield in cucumber
WO2025217398A1 (en) 2024-04-10 2025-10-16 Lyell Immunopharma, Inc. Methods for culturing cells with improved culture medium
WO2025235388A1 (en) 2024-05-06 2025-11-13 Regeneron Pharmaceuticals, Inc. Transgene genomic identification by nuclease-mediated long read sequencing
US20250345431A1 (en) 2024-05-10 2025-11-13 Juno Therapeutics, Inc. Genetically engineered t cells expressing a cd19 chimeric antigen receptor (car) and uses thereof for allogeneic cell therapy
US20250382627A1 (en) 2024-06-13 2025-12-18 J.R. Simplot Company Engineering remontant flowering in rosaceae
WO2025265017A1 (en) 2024-06-20 2025-12-26 Regeneron Pharmaceuticals, Inc. Ass1 gene insertion for the treatment of citrullinemia type i
WO2026017833A1 (en) 2024-07-17 2026-01-22 Vilmorin & Cie Tswv-resistant pepper
WO2026072529A1 (en) 2024-09-24 2026-04-02 University Of Florida Research Foundation, Incorporated Mettl7a for improved embryo competence
WO2026076425A1 (en) 2024-10-04 2026-04-09 Biodrive, Inc. Oral biodelivery platform
WO2026083329A1 (en) 2024-10-18 2026-04-23 Precision Biosciences, Inc. Methods of nuclease-initiated homology directed repair and replacement and compositions and uses thereof
WO2026083328A1 (en) 2024-10-18 2026-04-23 Precision Biosciences, Inc. Base editing nucleotide sequences using homology directed repair
WO2026123001A2 (en) 2024-12-06 2026-06-11 Umoja Biopharma, Inc. Lineage-loci engineered cell therapy and related methods of producing and using same
WO2026136848A2 (en) 2024-12-20 2026-06-25 Juno Therapeutics, Inc. Cd70-targeted cars and engineered cells comprising same and related methods
US20260223797A1 (en) 2025-01-31 2026-08-06 Harmoniz LTD Cucumber plants with small fruit and increased number of fruit

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030232410A1 (en) * 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942227A (en) * 1982-01-11 1990-07-17 California Institute Of Technology Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid, their preparation and use to cleave DNA
US4665184A (en) * 1983-10-12 1987-05-12 California Institute Of Technology Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid
US4795700A (en) * 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
US5789155A (en) * 1987-10-30 1998-08-04 California Institute Of Technology Process for identifying nucleic acids and triple helices formed thereby
US5955341A (en) * 1991-04-10 1999-09-21 The Scripps Research Institute Heterodimeric receptor libraries using phagemids
US5487994A (en) * 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5792640A (en) * 1992-04-03 1998-08-11 The Johns Hopkins University General method to clone hybrid restriction endonucleases using lig gene
US5916794A (en) * 1992-04-03 1999-06-29 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
US5356802A (en) * 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
US6331658B1 (en) * 1993-04-20 2001-12-18 Integris Baptist Medical Center, Inc. Genetically engineered mammals for use as organ donors
US6242568B1 (en) * 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) * 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
CA2196419C (en) * 1994-08-20 2007-08-21 Yen Choo Improvements in or relating to binding proteins for recognition of dna
US6326166B1 (en) * 1995-12-29 2001-12-04 Massachusetts Institute Of Technology Chimeric DNA-binding proteins
US5789538A (en) * 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
AU6096296A (en) 1995-06-07 1996-12-30 Ohio State University, The Artificial restriction endonuclease
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US6265196B1 (en) * 1996-05-07 2001-07-24 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US6781035B1 (en) * 1997-02-21 2004-08-24 The Regents Of The University Of California Leafy cotyledon1 genes and their uses
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
AU757930B2 (en) 1997-12-01 2003-03-13 Roche Diagnostics Gmbh Optimization of cells for endogenous gene activation
DE69942334D1 (en) * 1998-03-02 2010-06-17 Massachusetts Inst Technology POLY ZINC FINGER PROTEINS WITH IMPROVED LINKERS
JP2002522096A (en) 1998-08-12 2002-07-23 パンジーン・コーポレイション Development of domain-specific genes
US6140081A (en) * 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
BR9915191A (en) 1998-11-10 2001-12-11 Maxygen Inc Methods to obtain an isolated polynucleotide and to produce a recombinant cell, plant cell protoplast, collection of plant cell, plant, polynucleotide, library, one or more library members and target recombinant cell
US6534261B1 (en) * 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6503717B2 (en) 1999-12-06 2003-01-07 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
US6599692B1 (en) 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
AU2982900A (en) * 1999-02-03 2000-08-25 Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
WO2000046385A1 (en) 1999-02-03 2000-08-10 The Children's Medical Center Corporation Gene repair involving in vivo excision of targeting dna
AU4657500A (en) 1999-04-21 2000-11-02 Pangene Corporation Locked nucleic acid hybrids and methods of use
US6410317B1 (en) 1999-07-14 2002-06-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
US7151201B2 (en) * 2000-01-21 2006-12-19 The Scripps Research Institute Methods and compositions to modulate expression in plants
US7205103B2 (en) * 2000-02-11 2007-04-17 The Salk Institute For Biological Studies Method of regulating transcription in a cell
WO2001066717A2 (en) 2000-03-03 2001-09-13 The University Of Utah Gene targeting method
US6492117B1 (en) 2000-07-12 2002-12-10 Gendaq Limited Zinc finger polypeptides capable of binding DNA quadruplexes
US7091026B2 (en) * 2001-02-16 2006-08-15 University Of Iowa Research Foundation Artificial endonuclease
ATE347593T1 (en) 2002-01-23 2006-12-15 Univ Utah Res Found TARGETED CHROMOSOMAL MUTAGENESIS WITH ZINC FINGER NUCLEASES
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
JP2010500029A (en) 2006-08-11 2010-01-07 ダウ アグロサイエンシィズ エルエルシー Zinc finger nuclease-mediated homologous recombination
ES2585157T3 (en) 2007-09-27 2016-10-04 Dow Agrosciences Llc Designer zinc finger proteins that target the 5-enolpiruvil shikimato-3-phosphate synthase genes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030232410A1 (en) * 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BIBIKOVA ET AL.: "Enhancing gene targeting with designed zinc finger nucleases", SCIENCE, vol. 300, 2 May 2003 (2003-05-02), pages 764, XP002974267 *
BIBIKOVA ET AL.: "Stimulation of homologous recombination through targeted cleavage by chimeric nucleases", MOLECULAR AND CELLULAR BIOLOGY, vol. 21, no. 1, January 2001 (2001-01-01), pages 289 - 297, XP002974229 *
BIBIKOVA ET AL.: "Targeted chromosomal cleavage and mutagenesis in drosophila using zinc-finger nucleases", GENETICS, vol. 161, July 2002 (2002-07-01), pages 1169 - 1175, XP002261110 *
CHANDRASEGARAN ET AL.: "Chimeric restriction enzymes: what is next?", BIOLOGICAL CHEMISTRY, vol. 380, July 1999 (1999-07-01) - August 1999 (1999-08-01), pages 841 - 848, XP008019125 *
PORTEUS AND BALTIMORE: "GFP Gene Targeting System", pages 1 - 12, XP002974232, Retrieved from the Internet <URL:www.sciencemag.org/cgi/content/full/300/5620/763/DC1> *
PORTEUS ET AL.: "Chimeric nucleases stimulate gene targeting in human cells", SCIENCE, vol. 300, 2 May 2003 (2003-05-02), pages 763, XP002974231 *
SEGAL D.J.: "The use of zinc finger peptides to study the role of specific factor binding sites in the chromatin environment", METHODS, vol. 26, 2002, pages 76 - 83, XP002974227 *
SMITH ET AL.: "Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains", NUCLEIC ACIDS RESEARCH, vol. 28, no. 17, 2000, pages 3361 - 3369, XP002974228 *
WILSON J.H.: "pointing fingers at te limiting step in gene targeting", NATURE BIOTECHNOLOGY, vol. 21, no. 7, July 2003 (2003-07-01), pages 759 - 760, XP002974230 *
XP002974268, Retrieved from the Internet <URL:www.sciencemag.org/cgi/content/full/300/5620/763/DC1> *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591592B2 (en) 2020-04-24 2023-02-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers
US11845932B2 (en) 2020-04-24 2023-12-19 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells via viral delivery

Also Published As

Publication number Publication date
DE60310202D1 (en) 2007-01-18
US8106255B2 (en) 2012-01-31
JP4968498B2 (en) 2012-07-04
BR0307383A (en) 2005-04-26
EP1476547B1 (en) 2006-12-06
ATE347593T1 (en) 2006-12-15
US9145565B2 (en) 2015-09-29
DE60310202T2 (en) 2007-11-22
US20160076045A1 (en) 2016-03-17
US20050208489A1 (en) 2005-09-22
JP2005519631A (en) 2005-07-07
EP1476547A2 (en) 2004-11-17
US20120219959A1 (en) 2012-08-30
CN100575485C (en) 2009-12-30
JP2009195256A (en) 2009-09-03
US20110014601A2 (en) 2011-01-20
BRPI0307383B1 (en) 2019-12-31
CN1622993A (en) 2005-06-01
EP1476547A4 (en) 2005-08-17
WO2003087341A2 (en) 2003-10-23
CA2474486C (en) 2013-05-14
US20200231979A1 (en) 2020-07-23
AU2003251286B2 (en) 2007-08-16
CA2474486A1 (en) 2003-10-23
AU2003251286A1 (en) 2003-10-27

Similar Documents

Publication Publication Date Title
ATE347593T1 (en) TARGETED CHROMOSOMAL MUTAGENESIS WITH ZINC FINGER NUCLEASES
WO2004037977A3 (en) Use of chimeric nucleases to stimulate gene targeting
WO2003080807A8 (en) Compositions and methods for suppressing eukaryotic gene expression
WO2003064625A3 (en) Oligonucleotide compositions with enhanced efficiency
WO2005049842A3 (en) Improved targeted dna insertion in plants
WO2003062394A3 (en) Methods and compositions for rna interference
AU2004802A (en) Methods of modifying eukaryotic cells
MX343591B (en) Methods of modifying eukaryotic cells.
WO2002026967A3 (en) Targeted gene correction by single-stranded oligodeoxynucleotides
EP2368982A3 (en) Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
WO2002000904A3 (en) Recombinant constructs and their use in reducing gene expression
PL1802750T3 (en) Phytases
EP2336305A3 (en) Cloning of cytochrome P450 genes from Nicotiana
WO2005089287A3 (en) Methods and compositions for the specific inhibition of gene expression by double-stranded rna
MXPA06000092A (en) Chloroplast transformation of duckweed.
EP2275546A3 (en) Enzymatic DNA molecules
AP2004003122A0 (en) Cloning of cytochrome P450 genes from nicotiana
WO2001066717A8 (en) Gene targeting method
SI1675954T1 (en) Nucleic acids useful for triggering tumor cell lethality
WO1999057282A3 (en) Pesticidal toxins and nucleotide sequences which encode these toxins
WO2003027261A3 (en) Methods and compositions for gene targeting by homologous recombination
WO2002008432A3 (en) Composition and method for increased meiotic recombination in plants
WO2002010364A3 (en) Methods for enhancing targeted gene alteration using oligonucleotides
MXPA02012557A (en) Nucleic acid sequences and methods for the modification of plant gene expression.
WO2002006495A3 (en) Mutant muscle-specific enhancers

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2003251286

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 10502565

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2474486

Country of ref document: CA

Ref document number: 20038026643

Country of ref document: CN

Ref document number: 2003584285

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003746527

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003746527

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 2003746527

Country of ref document: EP