WO2003087825A3 - Method, system and kit for detecting an analyte in a sample - Google Patents

Method, system and kit for detecting an analyte in a sample Download PDF

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Publication number
WO2003087825A3
WO2003087825A3 PCT/IL2003/000257 IL0300257W WO03087825A3 WO 2003087825 A3 WO2003087825 A3 WO 2003087825A3 IL 0300257 W IL0300257 W IL 0300257W WO 03087825 A3 WO03087825 A3 WO 03087825A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
particulates
sample
binding couple
fluid sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2003/000257
Other languages
French (fr)
Other versions
WO2003087825A2 (en
Inventor
David Varon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MATIS MEDICAL Inc
Original Assignee
MATIS MEDICAL Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MATIS MEDICAL Inc filed Critical MATIS MEDICAL Inc
Priority to JP2003584718A priority Critical patent/JP2005523434A/en
Priority to DE60317497T priority patent/DE60317497T2/en
Priority to US10/511,705 priority patent/US20050130223A1/en
Priority to EP03746394A priority patent/EP1497652B1/en
Priority to AU2003215881A priority patent/AU2003215881A1/en
Priority to CA002482849A priority patent/CA2482849A1/en
Publication of WO2003087825A2 publication Critical patent/WO2003087825A2/en
Publication of WO2003087825A3 publication Critical patent/WO2003087825A3/en
Priority to IL164592A priority patent/IL164592A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The present invention concerns a rapid and easy in vitro method of diagnosing an analyte in a sample obtained from a subject, without the need of any sophisticated equipment. The method of the invention comprises in general detection of an analyte in a fluid sample, the method comprising mixing the fluid sample with a reagent comprising a capturing agent which is a first member of a binding couple that can bind to an analyte, the analyte being a second member of the binding couple, such that if the analyte is present in the fluid sample, particulates of the binding couple are formed; treating said mixture so as to form on a solid substrate a thin layer of said particulates, if formed as a result of said mixing; obtaining an optical image of the thin layer; and analyzing said optical image so as to determine therefrom the absence or presence of particulates formed as a result of the association between the binding couple, the presence of particulates in the sample indicating the presence of said analyte in the sample; or to determine from said image at least one parameter of said particulates.
PCT/IL2003/000257 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample Ceased WO2003087825A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2003584718A JP2005523434A (en) 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample
DE60317497T DE60317497T2 (en) 2002-04-16 2003-03-27 METHOD, SYSTEM AND KIT FOR DETECTING AN ANALYTE IN A SAMPLE
US10/511,705 US20050130223A1 (en) 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample
EP03746394A EP1497652B1 (en) 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample
AU2003215881A AU2003215881A1 (en) 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample
CA002482849A CA2482849A1 (en) 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample
IL164592A IL164592A (en) 2002-04-16 2004-10-14 Method for detecting an analyte in a sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37243202P 2002-04-16 2002-04-16
US60/372,432 2002-04-16

Publications (2)

Publication Number Publication Date
WO2003087825A2 WO2003087825A2 (en) 2003-10-23
WO2003087825A3 true WO2003087825A3 (en) 2004-01-29

Family

ID=29250851

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2003/000257 Ceased WO2003087825A2 (en) 2002-04-16 2003-03-27 Method, system and kit for detecting an analyte in a sample

Country Status (8)

Country Link
US (1) US20050130223A1 (en)
EP (1) EP1497652B1 (en)
JP (1) JP2005523434A (en)
AT (1) ATE378598T1 (en)
AU (1) AU2003215881A1 (en)
CA (1) CA2482849A1 (en)
DE (1) DE60317497T2 (en)
WO (1) WO2003087825A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109146165B (en) * 2018-08-09 2021-11-26 安徽建筑大学 Tropism clustering analysis method for Huizhou landscape settlement
US12529094B2 (en) * 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894590B1 (en) 2018-12-10 2025-10-22 10X Genomics, Inc. Methods of using master / copy arrays for spatial detection
CN115932247A (en) * 2022-12-07 2023-04-07 南京九川科学技术有限公司 Digital immunoassay method for unmarked magnetic bead planar microscopic counting

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3770380A (en) * 1971-04-19 1973-11-06 Us Army Article and method for multiple immune adherence assay
US4692412A (en) * 1972-10-06 1987-09-08 Livingston Virginia W Method of preparing an autogenous vaccine
WO1995004930A1 (en) * 1993-08-06 1995-02-16 The Government Of The United States, Represented By The Secretary Of The Navy Optical immunoassay for microbial analytes using non-specific dyes
US5487112A (en) * 1990-02-20 1996-01-23 Board Of Regents, The University Of Texas System Method and apparatus for time-resolved measurements of lymphocyte function and aggregate structure using computer-automated microscopy
WO1996012966A1 (en) * 1994-10-19 1996-05-02 Temple University - Of The Commonwealth System Of Higher Education Agglutination test
US5541417A (en) * 1995-05-18 1996-07-30 Abbott Laboratories Quantative agglutination reaction analysis method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5602005A (en) * 1989-03-03 1997-02-11 The University Of Virginia Alumni Patents Foundation Primate intra-acrosomal sperm antigen for use in a contraceptive vaccine
US6159748A (en) * 1995-03-13 2000-12-12 Affinitech, Ltd Evaluation of autoimmune diseases using a multiple parameter latex bead suspension and flow cytometry
AU2121497A (en) * 1996-02-22 1997-09-10 Dexall Biomedical Labs, Inc. Non-captive substrate liquid phase immunoassay
IL132687A0 (en) * 1999-11-01 2001-03-19 Keren Mechkarim Ichilov Pnimit System and method for evaluating body fluid samples
US7172804B2 (en) * 2001-07-17 2007-02-06 Northwestern University Film-immobilized capture particles

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3770380A (en) * 1971-04-19 1973-11-06 Us Army Article and method for multiple immune adherence assay
US4692412A (en) * 1972-10-06 1987-09-08 Livingston Virginia W Method of preparing an autogenous vaccine
US5487112A (en) * 1990-02-20 1996-01-23 Board Of Regents, The University Of Texas System Method and apparatus for time-resolved measurements of lymphocyte function and aggregate structure using computer-automated microscopy
WO1995004930A1 (en) * 1993-08-06 1995-02-16 The Government Of The United States, Represented By The Secretary Of The Navy Optical immunoassay for microbial analytes using non-specific dyes
WO1996012966A1 (en) * 1994-10-19 1996-05-02 Temple University - Of The Commonwealth System Of Higher Education Agglutination test
US5541417A (en) * 1995-05-18 1996-07-30 Abbott Laboratories Quantative agglutination reaction analysis method

Also Published As

Publication number Publication date
AU2003215881A1 (en) 2003-10-27
CA2482849A1 (en) 2003-10-23
US20050130223A1 (en) 2005-06-16
WO2003087825A2 (en) 2003-10-23
EP1497652B1 (en) 2007-11-14
ATE378598T1 (en) 2007-11-15
DE60317497D1 (en) 2007-12-27
EP1497652A2 (en) 2005-01-19
JP2005523434A (en) 2005-08-04
DE60317497T2 (en) 2008-06-12

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