WO2003089663A3 - Methods for measuring enzyme activity - Google Patents

Methods for measuring enzyme activity Download PDF

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Publication number
WO2003089663A3
WO2003089663A3 PCT/GB2003/001669 GB0301669W WO03089663A3 WO 2003089663 A3 WO2003089663 A3 WO 2003089663A3 GB 0301669 W GB0301669 W GB 0301669W WO 03089663 A3 WO03089663 A3 WO 03089663A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme activity
measuring enzyme
methods
relates
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2003/001669
Other languages
French (fr)
Other versions
WO2003089663A2 (en
Inventor
John Gerard Whateley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GE Healthcare UK Ltd
Original Assignee
Amersham Biosciences UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences UK Ltd filed Critical Amersham Biosciences UK Ltd
Priority to CA2484467A priority Critical patent/CA2484467C/en
Priority to EP03722751A priority patent/EP1497452B1/en
Priority to JP2003586372A priority patent/JP4713835B2/en
Priority to AU2003229914A priority patent/AU2003229914A1/en
Priority to IL16434703A priority patent/IL164347A0/en
Priority to DE60336217T priority patent/DE60336217D1/en
Priority to AT03722751T priority patent/ATE500342T1/en
Publication of WO2003089663A2 publication Critical patent/WO2003089663A2/en
Publication of WO2003089663A3 publication Critical patent/WO2003089663A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/9015—Ligases (6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to fluorescence methods for measuring enzyme activity, in particular enzyme cleaving and joining activities. The invention also relates to fluorogenic substrates which are useful for measuring enzyme activity and as in vitro and in vivo imaging probes.
PCT/GB2003/001669 2002-04-19 2003-04-17 Methods for measuring enzyme activity Ceased WO2003089663A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA2484467A CA2484467C (en) 2002-04-19 2003-04-17 Fluorescence-based methods for measuring enzyme activity
EP03722751A EP1497452B1 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity
JP2003586372A JP4713835B2 (en) 2002-04-19 2003-04-17 Method for measuring enzyme activity
AU2003229914A AU2003229914A1 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity
IL16434703A IL164347A0 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity
DE60336217T DE60336217D1 (en) 2002-04-19 2003-04-17 METHOD FOR DETERMINING ENZYME ACTIVITY
AT03722751T ATE500342T1 (en) 2002-04-19 2003-04-17 METHOD FOR DETERMINING ENZYME ACTIVITY

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0208989.4A GB0208989D0 (en) 2002-04-19 2002-04-19 Methods for measuring enzyme activity
GB0208989.4 2002-04-19

Publications (2)

Publication Number Publication Date
WO2003089663A2 WO2003089663A2 (en) 2003-10-30
WO2003089663A3 true WO2003089663A3 (en) 2004-05-27

Family

ID=9935152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/001669 Ceased WO2003089663A2 (en) 2002-04-19 2003-04-17 Methods for measuring enzyme activity

Country Status (9)

Country Link
US (2) US7727739B2 (en)
EP (1) EP1497452B1 (en)
JP (1) JP4713835B2 (en)
AT (1) ATE500342T1 (en)
AU (1) AU2003229914A1 (en)
CA (1) CA2484467C (en)
DE (1) DE60336217D1 (en)
GB (2) GB0208989D0 (en)
WO (1) WO2003089663A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101372632B1 (en) 2011-08-26 2014-03-14 동국대학교 산학협력단 Method for analysis of xpg endonuclease activity

Families Citing this family (18)

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GB0113434D0 (en) * 2001-06-04 2001-07-25 Amersham Pharm Biotech Uk Ltd Quinacridone derivatives as labels for fluorescence detection of biological materials
JP4637565B2 (en) * 2004-11-02 2011-02-23 一般社団法人オンチップ・セロミクス・コンソーシアム Biological sample labeling and biological substance labeling method and biological substance inspection method
US7741431B2 (en) * 2005-02-01 2010-06-22 The United States Of America As Represented By The Secretary Of The Army Liposomes containing novel targeting and/or fusogenic peptides, preparations containing them and therapeutic use thereof
US20090220587A1 (en) * 2005-02-01 2009-09-03 United State Army Liposomal drug delivery constructs targeted by lipid-conjugated peptide ligands
EP1900822B1 (en) * 2005-05-24 2016-02-03 Suzuki, Miho Method for simultaneous analysis of multiple biological reactions or changes in in vivo conditions
CA2627628A1 (en) * 2005-10-28 2007-05-03 Iti Scotland Limited Novel fluorescent dyes and uses thereof
US8691939B2 (en) 2007-06-08 2014-04-08 General Electric Company Compositions, methods, and kits for assaying complement activation
US20110039289A1 (en) * 2008-03-14 2011-02-17 Pierre Graves Fluorogenic peptides and their method of production
JP2010215572A (en) * 2009-03-17 2010-09-30 Japan Health Science Foundation Pulpitis diagnostic marker and pulpitis diagnostic system
JP5697129B2 (en) * 2010-03-24 2015-04-08 国立大学法人埼玉大学 Enzyme activity measurement substrate using FRET and method for producing the same
WO2012110833A2 (en) 2011-02-18 2012-08-23 Almac Sciences (Scotland) Limited Fluorescence modulators
US9180209B2 (en) 2011-02-28 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
WO2012131345A2 (en) 2011-03-25 2012-10-04 Almac Sciences (Scotland) Limited Enzyme assays
CN103765215A (en) * 2011-06-07 2014-04-30 生命科技公司 Fluorogenic semiconductor nanocrystals
GB201122099D0 (en) 2011-12-22 2012-02-01 Almac Sciences Scotland Ltd Dyes
US9229005B2 (en) 2012-03-23 2016-01-05 Surmodics Ivd, Inc. Compositions and methods for in vitro diagnostic tests including sulfonic acid compound
RU2622753C1 (en) * 2016-11-17 2017-06-19 Федеральное государственное бюджетное учреждение "Национальный научно-практический центр сердечно-сосудистой хирургии имени А.Н. Бакулева" Министерства здравоохранения Российской Федерации (ФГБУ "ННПЦССХ им. А.Н. Бакулева" Минздрава России) Method for angiotensin converting enzyme activity determination in heart tissues
CN114605405B (en) * 2022-03-09 2023-09-05 吉林大学 Cell lipid drop fluorescence imaging probe based on quinacridone skeleton and application thereof

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US20010005752A1 (en) * 1998-12-21 2001-06-28 Manfred Auer Fluorescent dyes (AIDA) for solid phase and solution phase screening

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US20010005752A1 (en) * 1998-12-21 2001-06-28 Manfred Auer Fluorescent dyes (AIDA) for solid phase and solution phase screening
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101372632B1 (en) 2011-08-26 2014-03-14 동국대학교 산학협력단 Method for analysis of xpg endonuclease activity

Also Published As

Publication number Publication date
AU2003229914A1 (en) 2003-11-03
GB2388190A (en) 2003-11-05
WO2003089663A2 (en) 2003-10-30
JP2005527212A (en) 2005-09-15
EP1497452B1 (en) 2011-03-02
DE60336217D1 (en) 2011-04-14
GB0208989D0 (en) 2002-05-29
GB0308910D0 (en) 2003-05-21
EP1497452A2 (en) 2005-01-19
CA2484467C (en) 2012-01-10
CA2484467A1 (en) 2003-10-30
GB2388190B (en) 2004-11-17
ATE500342T1 (en) 2011-03-15
US20030228609A1 (en) 2003-12-11
JP4713835B2 (en) 2011-06-29
US7727739B2 (en) 2010-06-01
US20100184098A1 (en) 2010-07-22

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