WO2003093426A3 - In vitro mutagenesis, phenotyping, and gene mapping - Google Patents

In vitro mutagenesis, phenotyping, and gene mapping Download PDF

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Publication number
WO2003093426A3
WO2003093426A3 PCT/US2003/013625 US0313625W WO03093426A3 WO 2003093426 A3 WO2003093426 A3 WO 2003093426A3 US 0313625 W US0313625 W US 0313625W WO 03093426 A3 WO03093426 A3 WO 03093426A3
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WIPO (PCT)
Prior art keywords
phenotyping
gene mapping
homozygous
cells
cellular
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Ceased
Application number
PCT/US2003/013625
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French (fr)
Other versions
WO2003093426A2 (en
Inventor
David W Threadgill
Daekee Lee
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University of North Carolina at Chapel Hill
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University of North Carolina at Chapel Hill
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Filing date
Publication date
Application filed by University of North Carolina at Chapel Hill filed Critical University of North Carolina at Chapel Hill
Priority to AU2003231244A priority Critical patent/AU2003231244A1/en
Priority to IL16468703A priority patent/IL164687A0/en
Priority to EP03724380A priority patent/EP1501927A2/en
Priority to CA002484360A priority patent/CA2484360A1/en
Publication of WO2003093426A2 publication Critical patent/WO2003093426A2/en
Publication of WO2003093426A3 publication Critical patent/WO2003093426A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cellular libraries useful for in vitro phenotyping and gene mapping. In a representative approach, a method for preparing a homozygous cellular library includes the steps of providing a heterozygous cellular library comprising a plurality of isolated parent cells; including site-specific mitotic recombination in the plurality of isolated parent cells; culturing the plurality of isolated parent cells, whereby a population of daughter cells is produced; and selecting daughter cells comprising a homozygous genetic modification, whereby a homozygous cellular library is prepared.
PCT/US2003/013625 2002-05-02 2003-05-02 In vitro mutagenesis, phenotyping, and gene mapping Ceased WO2003093426A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2003231244A AU2003231244A1 (en) 2002-05-02 2003-05-02 In vitro mutagenesis, phenotyping, and gene mapping
IL16468703A IL164687A0 (en) 2002-05-02 2003-05-02 Cellular libraries and methods for the preparationthereof
EP03724380A EP1501927A2 (en) 2002-05-02 2003-05-02 In vitro mutagenesis, phenotyping, and gene mapping
CA002484360A CA2484360A1 (en) 2002-05-02 2003-05-02 In vitro mutagenesis, phenotyping, and gene mapping

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37786402P 2002-05-02 2002-05-02
US60/377,864 2002-05-02

Publications (2)

Publication Number Publication Date
WO2003093426A2 WO2003093426A2 (en) 2003-11-13
WO2003093426A3 true WO2003093426A3 (en) 2004-03-18

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PCT/US2003/013625 Ceased WO2003093426A2 (en) 2002-05-02 2003-05-02 In vitro mutagenesis, phenotyping, and gene mapping

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US (1) US7208317B2 (en)
EP (1) EP1501927A2 (en)
AU (1) AU2003231244A1 (en)
CA (1) CA2484360A1 (en)
IL (1) IL164687A0 (en)
WO (1) WO2003093426A2 (en)

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US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
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Also Published As

Publication number Publication date
IL164687A0 (en) 2005-12-18
AU2003231244A1 (en) 2003-11-17
US7208317B2 (en) 2007-04-24
US20040033596A1 (en) 2004-02-19
WO2003093426A2 (en) 2003-11-13
EP1501927A2 (en) 2005-02-02
CA2484360A1 (en) 2003-11-13

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