WO2003096018A3 - Kit zur assay-entwicklung und für serien-analysen - Google Patents

Kit zur assay-entwicklung und für serien-analysen Download PDF

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Publication number
WO2003096018A3
WO2003096018A3 PCT/EP2003/004717 EP0304717W WO03096018A3 WO 2003096018 A3 WO2003096018 A3 WO 2003096018A3 EP 0304717 W EP0304717 W EP 0304717W WO 03096018 A3 WO03096018 A3 WO 03096018A3
Authority
WO
WIPO (PCT)
Prior art keywords
carrier substrate
kit
immobilized
bonding partners
array
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/004717
Other languages
English (en)
French (fr)
Other versions
WO2003096018A2 (de
Inventor
Gert L Duveneck
Peter Oroszlan
Michael Pawlak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zeptosens AG
Original Assignee
Zeptosens AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeptosens AG filed Critical Zeptosens AG
Priority to EP03729981A priority Critical patent/EP1506403A2/de
Priority to AU2003242251A priority patent/AU2003242251A1/en
Priority to US10/514,166 priority patent/US20050163659A1/en
Publication of WO2003096018A2 publication Critical patent/WO2003096018A2/de
Publication of WO2003096018A3 publication Critical patent/WO2003096018A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Die Erfindung betrifft einen Kit zurAssay-Entwicklung und zur Durchführung einer Vielzahl von Analysen, umfassend - ein Trägersubstrat und - einen Aufsatzkörper, welche zusammen eine Anordnung einer Vielzahl von Probenbehältnissen, mit besagtem Trägersubstrat als Grundplatte, bilden, sowie - eine Vielzahl immobilisierter Bindungspartner für den Nachweis eines oder mehrerer Analyten in einer oder mehreren Proben in einem Bioaffinitätsassay, wobei besagte Bindungspartner auf dem Trägersubstrat innerhalb der Probenbehältnisse jeweils in zweidimensionalen Arrays von diskreten Messbereichen angeordnet immobilisiert sind, wobei - jeweils mindestens ein Messbereich eines Arrays oder eine Teilfläche innerhalb eines Arrays bzw. Probenbehältnisses auf dem Trägersubstrat zu einer Referenzierung vorgesehen ist und - die Oberflächendichte der immobilisierten Bindungspartner, bezogen auf die Fläche der Messbereiche, weniger als der Oberflächendichte einer vollständigen, d.h. flächenhaften, Monoschicht besagter Bindungspartner entspricht. Diese Zusammensetzung des erfindungsgemässen Kits ermöglicht überraschenderweise die Durchführung kompletter Messreihen auf einem einzigen Trägersubstrat. Die Erfindung betrifft auch ein analytisches System, in dem ein erfindungsgemässer Kit verwendet wird, sowie darauf basierende analytische Nachweisverfahren und deren Verwendung.
PCT/EP2003/004717 2002-05-13 2003-05-06 Kit zur assay-entwicklung und für serien-analysen Ceased WO2003096018A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03729981A EP1506403A2 (de) 2002-05-13 2003-05-06 Kit for the development of analysis in parallel
AU2003242251A AU2003242251A1 (en) 2002-05-13 2003-05-06 Kit for assay development and serial analysis
US10/514,166 US20050163659A1 (en) 2002-05-13 2003-05-06 Kit for assay development and serial analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH791/02 2002-05-13
CH7912002 2002-05-13

Publications (2)

Publication Number Publication Date
WO2003096018A2 WO2003096018A2 (de) 2003-11-20
WO2003096018A3 true WO2003096018A3 (de) 2004-03-18

Family

ID=29410159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/004717 Ceased WO2003096018A2 (de) 2002-05-13 2003-05-06 Kit zur assay-entwicklung und für serien-analysen

Country Status (4)

Country Link
US (1) US20050163659A1 (de)
EP (1) EP1506403A2 (de)
AU (1) AU2003242251A1 (de)
WO (1) WO2003096018A2 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7101508B2 (en) * 2002-07-31 2006-09-05 Agilent Technologies, Inc. Chemical array fabrication errors
AU2003258683A1 (en) * 2002-09-03 2004-03-29 Zeptosens Ag Analytical platform and detection method with analytes which are to be detected in a sample in the form of immobilized specific binding partners
FR2847593A1 (fr) * 2002-11-26 2004-05-28 St Microelectronics Sa Procede et dispositif de realisation d'une couche de pentoxyde de tantale sur un materiau porteur, en particulier du niture de titane, et circuit integre incorporant une couche de pentoxyde de tantale
DE102004022484B4 (de) * 2004-05-07 2007-12-20 P.A.L.M. Microlaser Technologies Ag Mikroskoptisch
DE102004023262B8 (de) * 2004-05-11 2013-01-17 Carl Zeiss Microimaging Gmbh Verfahren zur Bearbeitung einer Masse mittels Laserbestrahlung und Steuersystem
CA2475456A1 (en) 2004-07-20 2006-01-20 Biophys, Inc. Method and device to optimize analyte and antibody substrate binding by least energy adsorption
CA2475240A1 (en) * 2004-07-20 2006-01-20 Biophys, Inc. Method and device to measure dynamic internal calibration true dose response curves
US7830507B2 (en) * 2006-02-13 2010-11-09 Optopo Inc. Spatially patterned substrates for chemical and biological sensing
US9976192B2 (en) 2006-03-10 2018-05-22 Ldip, Llc Waveguide-based detection system with scanning light source
US9528939B2 (en) 2006-03-10 2016-12-27 Indx Lifecare, Inc. Waveguide-based optical scanning systems
US8675199B2 (en) 2006-03-10 2014-03-18 Plc Diagnostics, Inc. Waveguide-based detection system with scanning light source
US9423397B2 (en) 2006-03-10 2016-08-23 Indx Lifecare, Inc. Waveguide-based detection system with scanning light source
US8288157B2 (en) 2007-09-12 2012-10-16 Plc Diagnostics, Inc. Waveguide-based optical scanning systems
US7532128B2 (en) * 2006-10-25 2009-05-12 Alverix, Inc. Position sensitive indicator detection
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
US20130165335A1 (en) * 2008-12-29 2013-06-27 Peter Lea Multiplex measure of isotype antigen response
KR102213461B1 (ko) * 2013-02-15 2021-02-08 삼성전자 주식회사 무선 셀룰라 통신 시스템에서 단말 대 단말 통신 수행을 위한 전력 제어 및 다중화 방법 및 장치
US10018566B2 (en) 2014-02-28 2018-07-10 Ldip, Llc Partially encapsulated waveguide based sensing chips, systems and methods of use
WO2016138427A1 (en) 2015-02-27 2016-09-01 Indx Lifecare, Inc. Waveguide-based detection system with scanning light source
DE102016110750A1 (de) * 2016-06-10 2017-12-14 Leica Microsystems Cms Gmbh Trägermembran für die Lasermikrodissektion einer auf die Trägermembran aufgebrachten Probe, Lasermikrodissektionseinrichtung und Lasermikrodissektionsverfahren unter Verwendung einer solchen Trägermembran
US11346839B2 (en) * 2018-08-03 2022-05-31 Insingulo Ab Method for determining the interaction between a ligand and a receptor
CN111398239B (zh) * 2020-05-19 2022-11-25 威海精讯畅通电子科技有限公司 一种基于荧光淬灭法的溶解氧测量装置
CN115046927A (zh) * 2022-06-29 2022-09-13 湖南超亟检测技术有限责任公司 一种基于光栅扫描识别的单分子多联检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0345789A2 (de) * 1988-06-10 1989-12-13 Keith E. Taylor Verfahren und Vorrichtung zur Befestigung von biologisch nützlichen Stoffen an eine Festphase
EP0789242A2 (de) * 1996-02-06 1997-08-13 Roche Diagnostics GmbH Werkstoffe und Verfahren für Bindungsassays
WO2001092870A2 (de) * 2000-06-02 2001-12-06 Zeptosens Ag Kit und verfahren zur multianalytbestimmung
WO2002040998A2 (de) * 2000-11-17 2002-05-23 Zeptosens Ag Kit und verfahren zur multianalytbestimmung

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8803000D0 (en) * 1988-02-10 1988-03-09 Ekins Roger Philip Determination of ambient concentrations of several analytes
US5807755A (en) * 1987-08-06 1998-09-15 Multilyte Limited Determination of ambient concentrations of several analytes
US5604105B1 (en) * 1990-10-12 1999-08-24 Spectral Diagnostics Inc Method and device for diagnosingand distinguishing chest pain in early onset thereof
CA2069537A1 (en) * 1991-06-07 1992-12-08 Thomas A. Cook Multiple output referencing system for evanescent wave sensor
GB9326450D0 (en) * 1993-12-24 1994-02-23 Multilyte Ltd Binding assay
JP4663824B2 (ja) * 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド 多重化分子分析装置および方法
US6087102A (en) * 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0345789A2 (de) * 1988-06-10 1989-12-13 Keith E. Taylor Verfahren und Vorrichtung zur Befestigung von biologisch nützlichen Stoffen an eine Festphase
EP0789242A2 (de) * 1996-02-06 1997-08-13 Roche Diagnostics GmbH Werkstoffe und Verfahren für Bindungsassays
WO2001092870A2 (de) * 2000-06-02 2001-12-06 Zeptosens Ag Kit und verfahren zur multianalytbestimmung
WO2002040998A2 (de) * 2000-11-17 2002-05-23 Zeptosens Ag Kit und verfahren zur multianalytbestimmung

Also Published As

Publication number Publication date
EP1506403A2 (de) 2005-02-16
AU2003242251A1 (en) 2003-11-11
WO2003096018A2 (de) 2003-11-20
US20050163659A1 (en) 2005-07-28

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