WO2003102585A3 - Biomolecular kinetics method using a flow-through microarray - Google Patents

Biomolecular kinetics method using a flow-through microarray Download PDF

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Publication number
WO2003102585A3
WO2003102585A3 PCT/EP2003/005748 EP0305748W WO03102585A3 WO 2003102585 A3 WO2003102585 A3 WO 2003102585A3 EP 0305748 W EP0305748 W EP 0305748W WO 03102585 A3 WO03102585 A3 WO 03102585A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
molecules
analyte
methods
interaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/005748
Other languages
French (fr)
Other versions
WO2003102585A2 (en
Inventor
Beuningen Marinus Gerardus Van
Robby Ruijtenbeek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PamGene BV
Original Assignee
PamGene BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PamGene BV filed Critical PamGene BV
Priority to DE60309317T priority Critical patent/DE60309317T2/en
Priority to CA002487944A priority patent/CA2487944A1/en
Priority to AU2003273530A priority patent/AU2003273530A1/en
Priority to JP2004509419A priority patent/JP2005528612A/en
Priority to EP03740171A priority patent/EP1512012B1/en
Priority to US10/515,302 priority patent/US20060234229A1/en
Publication of WO2003102585A2 publication Critical patent/WO2003102585A2/en
Publication of WO2003102585A3 publication Critical patent/WO2003102585A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/557—Immunoassay; Biospecific binding assay; Materials therefor using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/40—Flow patterns using back flushing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)

Abstract

The present invention relates to methods for monitoring interactions between target-molecules and at least one analyte comprising: (a) contacting a plurality of target-molecules, said target-molecules being immobilized on a porous substrate, with a sample, said sample comprising at least one analyte; (b) incubating said target-molecules with said sample under conditions supporting target-molecule/analyte interaction; (c) monitoring said interaction as defined in step (b), said monitoring being in function of time of incubation as defined in step (b); (d) optionally determining at least one interaction parameter; (e) varying said conditions as defined in step (b) by varying a reaction parameter; and; repeating, at least once, steps (c) to (e). The present invention further relates to the uses of said methods as well as to the use of a porous substrate for the preparation of a microarray kit for carrying out said methods.
PCT/EP2003/005748 2002-06-03 2003-06-02 Biomolecular kinetics method using a flow-through microarray Ceased WO2003102585A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE60309317T DE60309317T2 (en) 2002-06-03 2003-06-02 BIOMOLECULAR KINETICS MEASUREMENT PROCEDURE USING A FLOW MICRO ARRAY
CA002487944A CA2487944A1 (en) 2002-06-03 2003-06-02 Biomolecular kinetics method using a flow-through microarray
AU2003273530A AU2003273530A1 (en) 2002-06-03 2003-06-02 Biomolecular kinetics method using a flow-through microarray
JP2004509419A JP2005528612A (en) 2002-06-03 2003-06-02 A novel method for monitoring biomolecular interactions
EP03740171A EP1512012B1 (en) 2002-06-03 2003-06-02 Biomolecular kinetics method using a flow-through microarray
US10/515,302 US20060234229A1 (en) 2002-06-03 2003-06-02 Novel method for monitoring biomolecular interactions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02447107.0 2002-06-03
EP02447107 2002-06-03

Publications (2)

Publication Number Publication Date
WO2003102585A2 WO2003102585A2 (en) 2003-12-11
WO2003102585A3 true WO2003102585A3 (en) 2004-04-08

Family

ID=29595070

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/005748 Ceased WO2003102585A2 (en) 2002-06-03 2003-06-02 Biomolecular kinetics method using a flow-through microarray

Country Status (8)

Country Link
US (1) US20060234229A1 (en)
EP (1) EP1512012B1 (en)
JP (1) JP2005528612A (en)
AT (1) ATE343789T1 (en)
AU (1) AU2003273530A1 (en)
CA (1) CA2487944A1 (en)
DE (1) DE60309317T2 (en)
WO (1) WO2003102585A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6800453B2 (en) 2001-01-23 2004-10-05 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
DE602005023286D1 (en) * 2004-03-31 2010-10-14 Kazuo Shinya PROCEDURE FOR BIOSUBSTANCE INTEGRATION, FOR STRUCTURE ANALYSIS OR / AND IDENTIFICATION USING A MARKING SUBSTANCE
AU2005241003B2 (en) * 2004-04-14 2011-05-19 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
US8178316B2 (en) * 2006-06-29 2012-05-15 President And Fellows Of Harvard College Evaluating proteins
JP4751261B2 (en) * 2006-07-18 2011-08-17 セイコーインスツル株式会社 Dissociation constant measurement apparatus, dissociation constant calculation method, and dissociation constant calculation program
WO2008015645A2 (en) * 2006-08-02 2008-02-07 Koninklijke Philips Electronics N.V. A method of determining the concentration of an analyte using analyte sensor molecules coupled to a porous membrane
JP2010507384A (en) * 2006-10-27 2010-03-11 ジヤンセン・フアーマシユーチカ・ナームローゼ・フエンノートシヤツプ Methods for profiling kinase inhibitors
WO2009063408A1 (en) * 2007-11-16 2009-05-22 Nxp B.V. A biosensor device and a method of manufacturing the same
EP2103352A1 (en) 2008-03-18 2009-09-23 Koninklijke Philips Electronics N.V. Membranes suited for immobilizing biomolecules
EP2424971A4 (en) * 2009-05-01 2013-07-31 Univ Utah Res Found METHODS AND COMPOSITIONS FOR MEASURING HIGH-AFFINITY INTERACTIONS WITH SINGLE MOLECULE INTERACTION KINETICS (KISMI) IMAGING
EP2773964A4 (en) * 2011-11-03 2015-06-24 Stc Biolog Inc Method for determination of pharmacological properties of recombinant proteins
US20180292408A1 (en) * 2015-04-20 2018-10-11 Qoolabs, Inc. Camelid single-domain hcv antibodies and methods of use
US20180092993A1 (en) 2016-09-01 2018-04-05 Life Technologies Corporation Compositions and methods for enhanced fluorescence
ES2968625T3 (en) * 2017-07-27 2024-05-13 Univ Cornell A method for the attachment and retention of extracellular vesicles, fixed extracellular vesicles and a method for diagnosing a disease
AU2019343188A1 (en) 2018-09-21 2021-05-20 Aufbau Medical Innovations Limited Compositions and methods for glaucoma

Citations (5)

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Publication number Priority date Publication date Assignee Title
GB2261283A (en) * 1991-11-11 1993-05-12 Bunce Roger A Liquid transfer device for diagnostic assays
WO1997025619A1 (en) * 1996-01-11 1997-07-17 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Displacement assay on a porous membrane
US5726010A (en) * 1991-07-31 1998-03-10 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
WO1999002266A1 (en) * 1997-07-11 1999-01-21 Akzo Nobel N.V. A device for performing an assay, a method for manufacturing said device, and use of a membrane in the manufacture of said device
WO2000031304A1 (en) * 1998-11-25 2000-06-02 The Perkin-Elmer Corporation Methods and apparatus for flow-through hybridization

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US5324633A (en) * 1991-11-22 1994-06-28 Affymax Technologies N.V. Method and apparatus for measuring binding affinity
GB9416002D0 (en) * 1994-08-08 1994-09-28 Univ Cranfield Fluid transport device
US6287874B1 (en) * 1998-02-02 2001-09-11 Signature Bioscience, Inc. Methods for analyzing protein binding events
US6303325B1 (en) * 1998-05-29 2001-10-16 Dade Behring Inc. Method for detecting analytes
US6383748B1 (en) * 1999-09-14 2002-05-07 Pamgene B.V. Analytical test device with substrate having oriented through going channels and improved methods and apparatus for using same
EP1246698B1 (en) * 2000-01-06 2006-09-20 Caliper Life Sciences, Inc. Ultra high throughput sampling and analysis systems and methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5726010A (en) * 1991-07-31 1998-03-10 Idexx Laboratories, Inc. Reversible flow chromatographic binding assay
GB2261283A (en) * 1991-11-11 1993-05-12 Bunce Roger A Liquid transfer device for diagnostic assays
WO1997025619A1 (en) * 1996-01-11 1997-07-17 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Displacement assay on a porous membrane
WO1999002266A1 (en) * 1997-07-11 1999-01-21 Akzo Nobel N.V. A device for performing an assay, a method for manufacturing said device, and use of a membrane in the manufacture of said device
WO2000031304A1 (en) * 1998-11-25 2000-06-02 The Perkin-Elmer Corporation Methods and apparatus for flow-through hybridization

Non-Patent Citations (1)

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Title
SAPSFORD K E ET AL: "Kinetics of antigen binding to arrays of antibodies in different sized spots", ANAL. CHEM.;ANALYTICAL CHEMISTRY NOV 15 2001, vol. 73, no. 22, 15 November 2001 (2001-11-15), pages 5518 - 5524, XP002263066 *

Also Published As

Publication number Publication date
US20060234229A1 (en) 2006-10-19
AU2003273530A1 (en) 2003-12-19
EP1512012B1 (en) 2006-10-25
DE60309317T2 (en) 2007-05-31
JP2005528612A (en) 2005-09-22
ATE343789T1 (en) 2006-11-15
CA2487944A1 (en) 2003-12-11
DE60309317D1 (en) 2006-12-07
EP1512012A2 (en) 2005-03-09
WO2003102585A2 (en) 2003-12-11

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