WO2003106684A3 - PROCESS FOR THE SIMULTANEOUS PRODUCTION OF SEVERAL PROTEINS; VECTORS AND CELLS USED IN THIS PROCESS - Google Patents

PROCESS FOR THE SIMULTANEOUS PRODUCTION OF SEVERAL PROTEINS; VECTORS AND CELLS USED IN THIS PROCESS Download PDF

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Publication number
WO2003106684A3
WO2003106684A3 PCT/NL2003/000432 NL0300432W WO03106684A3 WO 2003106684 A3 WO2003106684 A3 WO 2003106684A3 NL 0300432 W NL0300432 W NL 0300432W WO 03106684 A3 WO03106684 A3 WO 03106684A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
cell
vectors
cells used
simultaneous production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2003/000432
Other languages
French (fr)
Other versions
WO2003106684A2 (en
Inventor
Arie Pieter Otte
Arthur Leo Kruckeberg
Richard George Antonius Sewalt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chromagenics BV
Original Assignee
Chromagenics BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2489475A priority Critical patent/CA2489475C/en
Priority to AT03733632T priority patent/ATE500331T1/en
Priority to DE60336228T priority patent/DE60336228D1/en
Priority to NZ537073A priority patent/NZ537073A/en
Priority to DK03733632.8T priority patent/DK1513937T3/en
Priority to AU2003238719A priority patent/AU2003238719B2/en
Priority to JP2004513497A priority patent/JP4647309B2/en
Application filed by Chromagenics BV filed Critical Chromagenics BV
Priority to EP03733632A priority patent/EP1513937B1/en
Publication of WO2003106684A2 publication Critical patent/WO2003106684A2/en
Publication of WO2003106684A3 publication Critical patent/WO2003106684A3/en
Priority to US11/013,031 priority patent/US7364878B2/en
Anticipated expiration legal-status Critical
Priority to US11/978,134 priority patent/US7901908B2/en
Priority to US11/978,109 priority patent/US7960143B2/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne le domaine de la biochimie, de la biologie moléculaire, de la pharmacologie et du diagnostic. Plus particulièrement, cette invention concerne la production de protéines dans une cellule hôte, et plus précisément, encore, un procédé permettant d'améliorer l'expression d'au moins deux protéines dans une cellule (hôte). Ledit procédé convient à la production, par exemple, d'anticorps de recombinaison pouvant être utilisés dans une préparation pharmaceutique ou en tant qu'outil diagnostique. Un mode de réalisation concerne un procédé permettant d'obtenir une cellule exprimant au moins deux protéines, lequel procédé consiste à introduire dans la cellule au moins deux unités d'expression protéique codant pour lesdites protéines. Ces unités d'expression protéique présentent la caractéristique de contenir au moins une séquence STAR.The present invention relates to the field of biochemistry, molecular biology, pharmacology and diagnostics. More particularly, this invention relates to the production of proteins in a host cell, and more specifically, a method for improving the expression of at least two proteins in a cell (host). Said method is suitable for the production, for example, of recombinant antibodies which can be used in a pharmaceutical preparation or as a diagnostic tool. One embodiment relates to a method making it possible to obtain a cell expressing at least two proteins, which method comprises introducing into the cell at least two protein expression units coding for said proteins. These protein expression units have the characteristic of containing at least one STAR sequence.

PCT/NL2003/000432 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein Ceased WO2003106684A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
AU2003238719A AU2003238719B2 (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins, vectors and cells for use therein
AT03733632T ATE500331T1 (en) 2002-06-14 2003-06-13 METHOD FOR THE SIMULTANEOUS PRODUCTION OF MULTIPLE PROTEINS; VECTORS AND CELLS USED FOR THIS
DE60336228T DE60336228D1 (en) 2002-06-14 2003-06-13 PROCESS FOR THE SIMULTANEOUS PREPARATION OF MULTIPLE PROTEINS; VECTORS AND CELLS THAT APPLY TO THEM
NZ537073A NZ537073A (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
DK03733632.8T DK1513937T3 (en) 2002-06-14 2003-06-13 Process for the simultaneous production of multiple proteins; vectors and cells for use therewith
JP2004513497A JP4647309B2 (en) 2002-06-14 2003-06-13 Methods for simultaneous production of multiple proteins; vectors and cells for use therein
EP03733632A EP1513937B1 (en) 2002-06-14 2003-06-13 A method for the stimultaneaous production of multiple proteins; vectors and cells for use therein
CA2489475A CA2489475C (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein
US11/013,031 US7364878B2 (en) 2002-06-14 2004-12-14 Method for simultaneous production of multiple proteins; vectors and cells for use therein
US11/978,109 US7960143B2 (en) 2002-06-14 2007-10-26 Method for simultaneous production of multiple proteins; vectors and cells for use therein
US11/978,134 US7901908B2 (en) 2002-06-14 2007-10-26 Method for simultaneous production of multiple proteins; vectors and cells for use therein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02077350.3 2002-06-14
EP02077350 2002-06-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/013,031 Continuation US7364878B2 (en) 2002-06-14 2004-12-14 Method for simultaneous production of multiple proteins; vectors and cells for use therein

Publications (2)

Publication Number Publication Date
WO2003106684A2 WO2003106684A2 (en) 2003-12-24
WO2003106684A3 true WO2003106684A3 (en) 2004-04-08

Family

ID=29724494

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2003/000432 Ceased WO2003106684A2 (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Country Status (11)

Country Link
US (3) US7364878B2 (en)
EP (1) EP1513937B1 (en)
JP (3) JP4647309B2 (en)
AT (1) ATE500331T1 (en)
AU (1) AU2003238719B2 (en)
CA (3) CA2489475C (en)
DE (1) DE60336228D1 (en)
DK (1) DK1513937T3 (en)
ES (1) ES2362273T3 (en)
NZ (1) NZ537073A (en)
WO (1) WO2003106684A2 (en)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
US8945876B2 (en) 2011-11-23 2015-02-03 University Of Hawaii Auto-processing domains for polypeptide expression

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KR101271884B1 (en) * 2004-11-08 2013-06-05 크로마제닉스 비.브이. Selection of host cells expressing protein at high levels
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US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8945876B2 (en) 2011-11-23 2015-02-03 University Of Hawaii Auto-processing domains for polypeptide expression

Also Published As

Publication number Publication date
US7901908B2 (en) 2011-03-08
JP5095693B2 (en) 2012-12-12
US7364878B2 (en) 2008-04-29
ES2362273T3 (en) 2011-06-30
US20080227199A1 (en) 2008-09-18
EP1513937B1 (en) 2011-03-02
AU2003238719B2 (en) 2008-02-21
ATE500331T1 (en) 2011-03-15
DK1513937T3 (en) 2011-05-16
CA2489475A1 (en) 2003-12-24
JP2010011859A (en) 2010-01-21
US7960143B2 (en) 2011-06-14
US20050191723A1 (en) 2005-09-01
CA2723500A1 (en) 2003-12-24
JP2010004886A (en) 2010-01-14
CA2723500C (en) 2014-10-28
AU2003238719A1 (en) 2003-12-31
DE60336228D1 (en) 2011-04-14
CA2722998C (en) 2014-12-02
CA2489475C (en) 2011-03-29
WO2003106684A2 (en) 2003-12-24
JP5095690B2 (en) 2012-12-12
CA2722998A1 (en) 2003-12-24
NZ537073A (en) 2006-08-31
EP1513937A2 (en) 2005-03-16
US20080227151A1 (en) 2008-09-18
JP4647309B2 (en) 2011-03-09
JP2005529610A (en) 2005-10-06

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