WO2004001042A3 - Microarrays displaying encoded molecules - Google Patents

Microarrays displaying encoded molecules Download PDF

Info

Publication number
WO2004001042A3
WO2004001042A3 PCT/DK2003/000417 DK0300417W WO2004001042A3 WO 2004001042 A3 WO2004001042 A3 WO 2004001042A3 DK 0300417 W DK0300417 W DK 0300417W WO 2004001042 A3 WO2004001042 A3 WO 2004001042A3
Authority
WO
WIPO (PCT)
Prior art keywords
encoded molecules
molecule
template
codes
displaying encoded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2003/000417
Other languages
French (fr)
Other versions
WO2004001042A2 (en
Inventor
Per-Ola Freskgaard
Henrik Pedersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nuevolution AS
Original Assignee
Nuevolution AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/DK2002/000419 external-priority patent/WO2002103008A2/en
Priority claimed from US10/175,539 external-priority patent/US7727713B2/en
Application filed by Nuevolution AS filed Critical Nuevolution AS
Priority to US10/518,056 priority Critical patent/US20060234231A1/en
Priority to AU2003240436A priority patent/AU2003240436A1/en
Priority to EP03729909A priority patent/EP1520040A2/en
Publication of WO2004001042A2 publication Critical patent/WO2004001042A2/en
Publication of WO2004001042A3 publication Critical patent/WO2004001042A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Disclosed is a microarray comprising a plurality of single stranded nucleic acid probes immobilized in discrete areas of a solid support, said probes being hybridised to a library of complexes, wherein each complex comprises an encoded molecule and a template which codes for said molecule, said template comprising a number of codons which codes for chemical entities which upon reaction form a reaction product which at least partly form part of the encoded molecule.
PCT/DK2003/000417 2001-06-20 2003-06-20 Microarrays displaying encoded molecules Ceased WO2004001042A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/518,056 US20060234231A1 (en) 2001-06-20 2003-06-20 Microarrays displaying encoded molecules
AU2003240436A AU2003240436A1 (en) 2002-06-20 2003-06-20 Microarrays displaying encoded molecules
EP03729909A EP1520040A2 (en) 2002-06-20 2003-06-20 Microarrays displaying encoded molecules

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
PCT/DK2002/000419 WO2002103008A2 (en) 2001-06-20 2002-06-20 Templated molecules and methods for using such molecules
US10/175,539 US7727713B2 (en) 2001-06-20 2002-06-20 Templated molecules and methods for using such molecules
US10/175,539 2002-06-20
DKPCT/DK02/00419 2002-06-20
DKPA200201953 2002-12-19
DKPA200201953 2002-12-19
US43466402P 2002-12-20 2002-12-20
US60/434,664 2002-12-20

Publications (2)

Publication Number Publication Date
WO2004001042A2 WO2004001042A2 (en) 2003-12-31
WO2004001042A3 true WO2004001042A3 (en) 2004-03-18

Family

ID=30003869

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000417 Ceased WO2004001042A2 (en) 2001-06-20 2003-06-20 Microarrays displaying encoded molecules

Country Status (3)

Country Link
EP (1) EP1520040A2 (en)
AU (1) AU2003240436A1 (en)
WO (1) WO2004001042A2 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223545B2 (en) 2001-03-19 2007-05-29 President And Fellows Of Harvard College Evolving new molecular function
US7491494B2 (en) 2002-08-19 2009-02-17 President And Fellows Of Harvard College Evolving new molecular function
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10731151B2 (en) 2002-03-15 2020-08-04 Nuevolution A/S Method for synthesising templated molecules
WO2004013070A2 (en) 2002-08-01 2004-02-12 Nuevolution A/S Multi-step synthesis of templated molecules
US7915201B2 (en) 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
WO2005026387A1 (en) 2003-09-18 2005-03-24 Nuevolution A/S A method for obtaining structural information concerning an encoded molecule and method for selecting compounds
RU2470077C2 (en) 2003-12-17 2012-12-20 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Biologically active compound containing coding oligonucleotide (versions), method for synthesis thereof, library of compounds (versions), method for synthesis thereof, and method of searching for compound bound with biological target (versions)
US7972994B2 (en) 2003-12-17 2011-07-05 Glaxosmithkline Llc Methods for synthesis of encoded libraries
WO2005090566A2 (en) 2004-03-22 2005-09-29 Nuevolution A/S Ligational encoding using building block oligonucleotides
WO2006095941A1 (en) * 2005-03-05 2006-09-14 Seegene, Inc. Processes using dual specificity oligonucleotide and dual specificity oligonucleotide
EP1940755A2 (en) 2005-10-28 2008-07-09 Praecis Pharmaceuticals Inc. Methods for identifying compounds of interest using encoded libraries
GB0712245D0 (en) * 2007-06-25 2007-08-01 Cythera Technologies Ltd Combinatorial libraries and related methods
KR102231531B1 (en) 2009-02-13 2021-03-23 엑스-켐, 인크. Methods of creating and screening dna-encoded libraries
CA2832672A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
ES2675111T3 (en) 2011-09-07 2018-07-06 X-Chem, Inc. Methods for tagging libraries with DNA coding
CN104685115A (en) 2012-07-13 2015-06-03 X-化学有限公司 DNA-encoded library with polymerase-unreadable coding oligonucleotide ligation
JP6262848B2 (en) 2013-05-21 2018-01-17 成都先導薬物開発有限公司 How to capture drug targets

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994008051A1 (en) * 1992-10-01 1994-04-14 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
WO1996041011A1 (en) * 1995-06-07 1996-12-19 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
EP0778280A2 (en) * 1993-07-30 1997-06-11 Isis Innovation Limited Tag reagent and assay method
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
US5723598A (en) * 1992-03-30 1998-03-03 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1999042605A1 (en) * 1998-02-21 1999-08-26 Schwabacher Alan W One dimensional chemical compound arrays and methods for assaying them
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0971946T3 (en) * 1997-01-21 2006-10-30 Gen Hospital Corp Selection of proteins using RNA-protein fusions
US6261804B1 (en) * 1997-01-21 2001-07-17 The General Hospital Corporation Selection of proteins using RNA-protein fusions

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723598A (en) * 1992-03-30 1998-03-03 The Scripps Research Institute Encoded combinatorial chemical libraries
WO1994008051A1 (en) * 1992-10-01 1994-04-14 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
EP0778280A2 (en) * 1993-07-30 1997-06-11 Isis Innovation Limited Tag reagent and assay method
WO1996041011A1 (en) * 1995-06-07 1996-12-19 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
WO1997027317A1 (en) * 1996-01-23 1997-07-31 Affymetrix, Inc. Nucleic acid analysis techniques
WO1999042605A1 (en) * 1998-02-21 1999-08-26 Schwabacher Alan W One dimensional chemical compound arrays and methods for assaying them
WO2000023458A1 (en) * 1998-10-19 2000-04-27 The Board Of Trustees Of The Leland Stanford Junior University Dna-templated combinatorial library chemistry

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRUICK R K ET AL: "TEMPLATE-DIRECTED LIGATION OF PEPTIDES TO OLIGONUCLEOTIDES", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 3, no. 1, January 1996 (1996-01-01), pages 49 - 56, XP000856876, ISSN: 1074-5521 *
LOCKHART D J ET AL: "EXPRESSION MONITORING BY HYBRIDIZATION TO HIGH-DENSITY OLIGONUCLEOTIDE ARRAYS", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, vol. 14, no. 13, 1 December 1996 (1996-12-01), pages 1675 - 1680, XP002022521, ISSN: 0733-222X *
See also references of EP1520040A2 *
SUMMERER D ET AL: "DNA-TEMPLATED SYNTHESIS: MORE VERSATILE THAN EXPECTED", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, VERLAG CHEMIE. WEINHEIM, DE, vol. 41, no. 1, 4 January 2002 (2002-01-04), pages 89 - 90, XP001170352, ISSN: 0570-0833 *
WALDER J A ET AL: "COMPLEMENTARY CARRIER PEPTIDE SYNTHESIS: GENERAL STRATEGY AND IMPLICATIONS FOR PREBIOTIC ORIGIN OF PEPTIDE SYNTHESIS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 76, no. 1, January 1979 (1979-01-01), pages 51 - 55, XP000857351, ISSN: 0027-8424 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7223545B2 (en) 2001-03-19 2007-05-29 President And Fellows Of Harvard College Evolving new molecular function
US7442160B2 (en) 2001-03-19 2008-10-28 President And Fellows Of Harvard College Evolving new molecular function
US7557068B2 (en) 2001-03-19 2009-07-07 President And Fellows Of Harvard College Evolving new molecular function
US8932992B2 (en) 2001-06-20 2015-01-13 Nuevolution A/S Templated molecules and methods for using such molecules
US8808984B2 (en) 2002-03-15 2014-08-19 Neuvolution A/S Method for synthesising templated molecules
US7491494B2 (en) 2002-08-19 2009-02-17 President And Fellows Of Harvard College Evolving new molecular function
US8206901B2 (en) 2002-10-30 2012-06-26 Nuevolution A/S Method for the synthesis of a bifunctional complex
US8722583B2 (en) 2002-10-30 2014-05-13 Nuevolution A/S Method for selecting a chemical entity from a tagged library
US9109248B2 (en) 2002-10-30 2015-08-18 Nuevolution A/S Method for the synthesis of a bifunctional complex
US9121110B2 (en) 2002-12-19 2015-09-01 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US9096951B2 (en) 2003-02-21 2015-08-04 Nuevolution A/S Method for producing second-generation library
US9574189B2 (en) 2005-12-01 2017-02-21 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries

Also Published As

Publication number Publication date
AU2003240436A1 (en) 2004-01-06
AU2003240436A8 (en) 2004-01-06
WO2004001042A2 (en) 2003-12-31
EP1520040A2 (en) 2005-04-06

Similar Documents

Publication Publication Date Title
EP2336315A3 (en) Enzymatic encoding methods for efficient synthesis of large libraries
Kumar et al. Silanized nucleic acids: a general platform for DNA immobilization
AU767606B2 (en) Method for producing polymers
WO2005090566A3 (en) Ligational encoding using building block oligonucleotides
Shibata et al. DNA-mediated enantioselective carbon-fluorine bond formation
IS2651B (en) Microcoding methods for multiple gene combinations show a variety of gene sites
WO2004016767A3 (en) Evolving new molecular function
WO2004074501A3 (en) A method for obtaining structural information about an encoded molecule
WO2002046450A3 (en) Method for generating a library of mutant oligonucleotides using the linear cyclic amplification reaction
IL163788A0 (en) Method of error reduction in nucleic acid populations
WO2006026614A3 (en) Method of error reduction in nucleic acid populations
WO2002031182A3 (en) Method and apparatus for reading reporter labeled beads
WO2006079061A3 (en) Free reactant use in nucleic acid-templated synthesis
Saboulard et al. High-throughput site-directed mutagenesis using oligonucleotides synthesized on DNA chips
WO2002018655A3 (en) Compositions and methods for the generation of single stranded cdna microarrays with accurate universal quantitation reference
Cronin et al. Utilization of new technologies in drug trials and discovery
WO2002007870A3 (en) Use of combinatorial chemistry to optimize multi-step synthesis
Trachtenberg et al. A primer on the current state of microarray technologies
WO2002087754A3 (en) Method for conducting solid phase synthesis of molecule libraries using combinatorial sealing matrices
Borman New alternative to bead synthesis
EP2883963A1 (en) Method for generating of oligonucleotide arrays using in situ block synthesis approach
WO1998049341A3 (en) Polynucleotide sequencing using semi-degenerate primers
WO2002086441A3 (en) Amplification and detection of mycoplasma pneumoniae
Tullo Quick discovery
WO2003066549A3 (en) Synthesis of sulfamidates

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003729909

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003729909

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2006234231

Country of ref document: US

Ref document number: 10518056

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 10518056

Country of ref document: US