WO2004002216A3 - Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof - Google Patents

Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof Download PDF

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Publication number
WO2004002216A3
WO2004002216A3 PCT/CA2003/000964 CA0300964W WO2004002216A3 WO 2004002216 A3 WO2004002216 A3 WO 2004002216A3 CA 0300964 W CA0300964 W CA 0300964W WO 2004002216 A3 WO2004002216 A3 WO 2004002216A3
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WO
WIPO (PCT)
Prior art keywords
alfalfa
harvest
methods
medicago sativa
genes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CA2003/000964
Other languages
French (fr)
Other versions
WO2004002216A2 (en
Inventor
Larry Erickson
Jian Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Guelph
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University of Guelph
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Guelph filed Critical University of Guelph
Priority to EP03737803A priority Critical patent/EP1517605A2/en
Priority to AU2003245772A priority patent/AU2003245772A1/en
Priority to CA002490781A priority patent/CA2490781A1/en
Priority to US10/519,811 priority patent/US7388091B2/en
Priority to US10/745,721 priority patent/US7329798B2/en
Publication of WO2004002216A2 publication Critical patent/WO2004002216A2/en
Publication of WO2004002216A3 publication Critical patent/WO2004002216A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to novel inducible genes from alfalfa. These genes are highly induced following mechanical harvesting or wounding of alfalfa. The genes are useful for inducible production of heterologous proteins following harvesting.
PCT/CA2003/000964 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof Ceased WO2004002216A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP03737803A EP1517605A2 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof
AU2003245772A AU2003245772A1 (en) 2002-06-28 2003-06-27 Harvest-inducible genes form alfalfa (medicago sativa) and methods of use thereof
CA002490781A CA2490781A1 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof
US10/519,811 US7388091B2 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (Medicago sativa) and methods of use thereof
US10/745,721 US7329798B2 (en) 2002-06-28 2003-12-23 Harvest-inducible regulatory elements and methods of using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39244402P 2002-06-28 2002-06-28
US60/392,444 2002-06-28

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/745,721 Continuation-In-Part US7329798B2 (en) 2002-06-28 2003-12-23 Harvest-inducible regulatory elements and methods of using same

Publications (2)

Publication Number Publication Date
WO2004002216A2 WO2004002216A2 (en) 2004-01-08
WO2004002216A3 true WO2004002216A3 (en) 2004-07-01

Family

ID=30000871

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/000964 Ceased WO2004002216A2 (en) 2002-06-28 2003-06-27 Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof

Country Status (5)

Country Link
US (1) US7388091B2 (en)
EP (1) EP1517605A2 (en)
AU (1) AU2003245772A1 (en)
CA (1) CA2490781A1 (en)
WO (1) WO2004002216A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329798B2 (en) * 2002-06-28 2008-02-12 University Of Guelph Harvest-inducible regulatory elements and methods of using same
DE102004057291C5 (en) * 2004-11-26 2010-08-26 Südzucker AG Mannheim/Ochsenfurt Storage-induced promoters
CN115399286B (en) * 2022-07-21 2024-05-28 广西壮族自治区畜牧研究所 A livestock breeding method using alfalfa powder as feed

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001073090A2 (en) * 2000-03-24 2001-10-04 The Samuel Roberts Noble Foundation, Inc. Method for modifying lignin composition and increasing in vivo digestibility of forages

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8300698A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; AGROBACTERIUM TUMEFACIENS BACTERIA AND METHOD FOR PRODUCTION THEREOF; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
NL8300699A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; METHOD FOR PRODUCING AGROBACTERIUM TUMEFACIENS BACTERIEN; STABLE COINTEGRATE PLASMIDS; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
US5504200A (en) 1983-04-15 1996-04-02 Mycogen Plant Science, Inc. Plant gene expression
DE3321346C2 (en) 1983-06-13 1987-04-09 Philips Patentverwaltung Gmbh, 2000 Hamburg Optical print head
JPS6083584A (en) 1983-10-13 1985-05-11 Rikagaku Kenkyusho Method for transferring substances into living cells
US4784949A (en) 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US5231019A (en) 1984-05-11 1993-07-27 Ciba-Geigy Corporation Transformation of hereditary material of plants
US5149645A (en) 1984-06-04 1992-09-22 Rijksuniversiteit Leiden Process for introducing foreign DNA into the genome of plants
US4743548A (en) 1984-09-25 1988-05-10 Calgene, Inc. Plant cell microinjection technique
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1340766C (en) 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
ATE57390T1 (en) 1986-03-11 1990-10-15 Plant Genetic Systems Nv PLANT CELLS OBTAINED BY GENOLOGICAL TECHNOLOGY AND RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS.
US5120657A (en) 1986-12-05 1992-06-09 Agracetus, Inc. Apparatus for genetic transformation
AU2811889A (en) 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
US5179022A (en) 1988-02-29 1993-01-12 E. I. Du Pont De Nemours & Co. Biolistic apparatus for delivering substances into cells and tissues in a non-lethal manner
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
CA1339307C (en) 1988-09-06 1997-08-19 Roy Curtiss, Iii Oral immunization by transgenic plants
DE3843627A1 (en) 1988-12-21 1990-07-05 Inst Genbiologische Forschung POTATO TUBE-SPECIFIC TRANSCRIPTIONAL REGULATION
US5723757A (en) 1988-12-21 1998-03-03 Hoechst Schering Agrevo Gmbh Plant promoters specific for sink organ expression of genes
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
NL8901932A (en) 1989-07-26 1991-02-18 Mogen Int PRODUCTION OF heterologous PROTEINS IN PLANTS OR PLANTS.
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US5202422A (en) 1989-10-27 1993-04-13 The Scripps Research Institute Compositions containing plant-produced glycopolypeptide multimers, multimeric proteins and method of their use
JPH04506155A (en) 1990-03-16 1992-10-29 カルジーン,インコーポレイティド Novel sequences preferentially expressed in early seed formation and related methods
US5543576A (en) 1990-03-23 1996-08-06 Mogen International Production of enzymes in seeds and their use
ES2260886T3 (en) 1990-11-23 2006-11-01 Bayer Bioscience N.V. PROCEDURE FOR TRANSFORMING MONOCOTILEDONE PLANTS.
US5948682A (en) 1991-02-22 1999-09-07 Sembiosys Genetics Inc. Preparation of heterologous proteins on oil bodies
IL101508A0 (en) 1991-04-08 1992-12-30 Rhone Poulenc Agrochimie Chimeric plant genes based on upstream regulatory elements of helianthinin
US5612487A (en) 1991-08-26 1997-03-18 Edible Vaccines, Inc. Anti-viral vaccines expressed in plants
US6034298A (en) 1991-08-26 2000-03-07 Prodigene, Inc. Vaccines expressed in plants
US5484719A (en) 1991-08-26 1996-01-16 Edible Vaccines, Inc. Vaccines produced and administered through edible plants
AU678154B2 (en) 1992-04-02 1997-05-22 Sembiosys Genetics Inc. Oil-body protein cis-elements as regulatory signals
ATE398679T1 (en) 1992-07-07 2008-07-15 Japan Tobacco Inc METHOD FOR TRANSFORMING A MONOCOTYLEDON PLANT
US5670349A (en) * 1993-08-02 1997-09-23 Virginia Tech Intellectual Properties, Inc. HMG2 promoter expression system and post-harvest production of gene products in plants and plant cell cultures
DK0690732T3 (en) 1994-01-21 2003-05-19 Powderject Vaccines Inc Gas-powered re-entry instrument
US6046037A (en) 1994-12-30 2000-04-04 Hiatt; Andrew C. Method for producing immunoglobulins containing protection proteins in plants and their use
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
FR2736285B1 (en) 1995-07-07 1997-08-22 Valois DEVICE FOR DISPENSING FLUID PRODUCT WITH BACTERIOSTATIC ACTION
US5859327A (en) 1995-08-22 1999-01-12 Genetronics, Inc. Electroporation-mediated molecular transfer in intact plants
ES2326705T3 (en) 1995-09-14 2009-10-16 Virginia Tech Intellectual Properties, Inc. PRODUCTION OF LISOSOMIC ENZYMES IN EXPRESSION SYSTEMS BASED ON PLANTS.
US5824870A (en) 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
US5767379A (en) 1995-11-06 1998-06-16 John Howard Commercial production of avidin in plants
US5804694A (en) 1995-11-06 1998-09-08 Prodigene, Inc. Commercial production of β-glucuronidase in plants
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5981835A (en) 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes
CA2221843A1 (en) 1998-01-27 1999-07-27 Her Majesty The Queen, In Right Of Canada, As Represented By The Ministe Ture Porcine reproductive and respiratory syndrome oral vaccine production in plants
ES2336861T3 (en) 1998-06-22 2010-04-16 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food PRODUCTION OF IL-10 IN BIORREACTOR OF GROWTH PLANT NON-FOOD.
AU1418300A (en) 1998-11-26 2000-06-13 New Zealand Institute For Crop & Food Research Limited Control of post harvest gene expression in plants by the use of harvest-regulated gene promoters
JP3427036B2 (en) 2000-03-30 2003-07-14 富士通日立プラズマディスプレイ株式会社 Display panel driving method and panel display device
US6783394B1 (en) 2003-03-18 2004-08-31 Randall A. Holliday Universal multi-stage compression connector

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001073090A2 (en) * 2000-03-24 2001-10-04 The Samuel Roberts Noble Foundation, Inc. Method for modifying lignin composition and increasing in vivo digestibility of forages

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FERULLO JEAN-MARC ET AL: "Post-harvest alteration of in vitro translatable mRNA population in alfalfa (Medicago sativa L.)", CROP SCIENCE, CROP SCIENCE SOCIETY OF AMERICA, MADISON, WI, US, vol. 36, no. 4, 1996, pages 1011 - 1016, XP002146910, ISSN: 0011-183X *
KUHN EKKEHARD: "From library screening to microarray technology: Strategies to determine gene expression profiles and to identify differentially regulated genes in plants", ANNALS OF BOTANY (LONDON), vol. 87, no. 2, February 2001 (2001-02-01), pages 139 - 155, XP002259520, ISSN: 0305-7364 *
MATZ M V ET AL: "Different strategies of differential display: Areas of application", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 24, 15 December 1998 (1998-12-15), pages 5537 - 5543, XP002245467, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
AU2003245772A1 (en) 2004-01-19
CA2490781A1 (en) 2004-01-08
WO2004002216A2 (en) 2004-01-08
EP1517605A2 (en) 2005-03-30
US20060168676A1 (en) 2006-07-27
US7388091B2 (en) 2008-06-17

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