WO2004019038A3 - Verfahren zum nachweis von antikörpern und/oder antigenen in einer testflüssigkeit, insbesondere bei der blutgruppenbestimmung - Google Patents

Verfahren zum nachweis von antikörpern und/oder antigenen in einer testflüssigkeit, insbesondere bei der blutgruppenbestimmung Download PDF

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Publication number
WO2004019038A3
WO2004019038A3 PCT/EP2003/008995 EP0308995W WO2004019038A3 WO 2004019038 A3 WO2004019038 A3 WO 2004019038A3 EP 0308995 W EP0308995 W EP 0308995W WO 2004019038 A3 WO2004019038 A3 WO 2004019038A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigens
buffer
antibodies
mixture
test liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/008995
Other languages
English (en)
French (fr)
Other versions
WO2004019038A2 (de
Inventor
Joerg Spindler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Rotes Kreuz Blutspendendienst Baden-Wurt Gemeinnu GmbH
Original Assignee
Deutsches Rotes Kreuz Blutspendendienst Baden-Wurt Gemeinnu GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Rotes Kreuz Blutspendendienst Baden-Wurt Gemeinnu GmbH filed Critical Deutsches Rotes Kreuz Blutspendendienst Baden-Wurt Gemeinnu GmbH
Priority to AU2003260413A priority Critical patent/AU2003260413A1/en
Priority to CA2495728A priority patent/CA2495728C/en
Priority to EP03792313A priority patent/EP1532454A2/de
Publication of WO2004019038A2 publication Critical patent/WO2004019038A2/de
Publication of WO2004019038A3 publication Critical patent/WO2004019038A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum Nachweis von Antikörpern oder Antigenen in einer Testflüssigkeit durch Reaktion mit einem vorgegebenen spezifischen Bindungspartner, wobei die Antigene an einen Träger gebunden sind und das dergestalt hergestellte Probengemisch einem Inkubationsschritt ausgesetzt wird und anschließend durch ein Partikel-Puffer-Gemisch zentrifugiert wird, wobei bei positiver Antigen-Antikörper-Reaktion ein Agglutinat aus nachzuweisendem Antigen, Träger und Antikörpern gebildet wird, wobei eine vorbestimmte Menge des inkubierten Probengemisches auf eine vorbestimmte Menge eines Puffer-Partikel-Gemisches, welches sich innerhalb eines Nachweisgefäßes befindet, zugegeben wird, wobei das Puffer-Partikel-Gemisch einen Einbettpuffer aufweist, der aus einer wässrigen Lösung von Dextran und/oder Polyethylenglycol, Glycin und/oder Trisodiumcitrat besteht.
PCT/EP2003/008995 2002-08-23 2003-08-13 Verfahren zum nachweis von antikörpern und/oder antigenen in einer testflüssigkeit, insbesondere bei der blutgruppenbestimmung Ceased WO2004019038A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003260413A AU2003260413A1 (en) 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood type
CA2495728A CA2495728C (en) 2002-08-23 2003-08-13 Method for the detection of antibodies and/or antigens in a test liquid, particularly for determining the blood group
EP03792313A EP1532454A2 (de) 2002-08-23 2003-08-13 Verfahren zum nachweis von antikörpern und/oder antigenen in einer testflüssigkeit, insbesondere bei der blutgruppenbestimmung

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2002139568 DE10239568A1 (de) 2002-08-23 2002-08-23 Verfahren zum Nachweis von Antikörpern und/oder Antigenen in einer Testflüssigkeit sowie zur Blutgruppenbestimmung
DE10239568.3 2002-08-23

Publications (2)

Publication Number Publication Date
WO2004019038A2 WO2004019038A2 (de) 2004-03-04
WO2004019038A3 true WO2004019038A3 (de) 2004-04-22

Family

ID=31197475

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/008995 Ceased WO2004019038A2 (de) 2002-08-23 2003-08-13 Verfahren zum nachweis von antikörpern und/oder antigenen in einer testflüssigkeit, insbesondere bei der blutgruppenbestimmung

Country Status (5)

Country Link
EP (1) EP1532454A2 (de)
AU (1) AU2003260413A1 (de)
CA (1) CA2495728C (de)
DE (1) DE10239568A1 (de)
WO (1) WO2004019038A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006024927B4 (de) * 2006-05-25 2008-12-11 DRK-Blutspendedienst Baden-Württemberg-Hessen gemeinnützige GmbH Verfahren zum Nachweis von Antikörpern und/oder Antigenen sowie zur Blutgruppenbestimmung in einer Testsubstanz
CN102445550B (zh) * 2010-10-09 2014-02-05 苏州苏大赛尔免疫生物技术有限公司 一种ABO、RhD血型定型试剂卡及其制备方法
CN103926415B (zh) * 2014-03-24 2015-11-25 上海市血液中心 人血液中血型抗体联合快速检测体系
CN105628940A (zh) * 2015-12-30 2016-06-01 合肥天一生物技术研究所 一种abo亚型血型检测卡
DE102017114537A1 (de) * 2017-06-29 2019-01-03 Endress+Hauser Conducta Gmbh+Co. Kg Sensormembran, Sensorkappe und optischer Sensor

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552064A (en) * 1993-02-26 1996-09-03 Ortho Diagnostic Systems, Inc. Column agglutination assay and device using biphasic centrifugation
EP0849595A1 (de) * 1996-12-18 1998-06-24 Stiftung Für Diagnostische Forschung Synthetische Partikel als Agglutinationsreagenzien
ES2126521A1 (es) * 1997-06-05 1999-03-16 Transfusion De La Comunidad Va Metodo para la deteccion de antigenos presentes en la membrana de hematies, propios o acoplados y de anticuerpos irregulares en muestras de suero.
US5905028A (en) * 1994-05-17 1999-05-18 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5552064A (en) * 1993-02-26 1996-09-03 Ortho Diagnostic Systems, Inc. Column agglutination assay and device using biphasic centrifugation
US5905028A (en) * 1994-05-17 1999-05-18 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies
EP0849595A1 (de) * 1996-12-18 1998-06-24 Stiftung Für Diagnostische Forschung Synthetische Partikel als Agglutinationsreagenzien
ES2126521A1 (es) * 1997-06-05 1999-03-16 Transfusion De La Comunidad Va Metodo para la deteccion de antigenos presentes en la membrana de hematies, propios o acoplados y de anticuerpos irregulares en muestras de suero.

Also Published As

Publication number Publication date
AU2003260413A8 (en) 2004-03-11
CA2495728C (en) 2012-08-21
CA2495728A1 (en) 2004-03-04
AU2003260413A1 (en) 2004-03-11
EP1532454A2 (de) 2005-05-25
WO2004019038A2 (de) 2004-03-04
DE10239568A1 (de) 2004-03-04

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