WO2004022779A3 - Verfahren zum nachweis von nukleinsäuresequenzen mittels spaltbarer sondenmoleküle - Google Patents

Verfahren zum nachweis von nukleinsäuresequenzen mittels spaltbarer sondenmoleküle Download PDF

Info

Publication number
WO2004022779A3
WO2004022779A3 PCT/DE2003/002936 DE0302936W WO2004022779A3 WO 2004022779 A3 WO2004022779 A3 WO 2004022779A3 DE 0302936 W DE0302936 W DE 0302936W WO 2004022779 A3 WO2004022779 A3 WO 2004022779A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
probe molecules
crackable
acid sequences
hybridized
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2003/002936
Other languages
English (en)
French (fr)
Other versions
WO2004022779A2 (de
Inventor
Philipp Schatz
Matthias Schuster
Kurt Berlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Priority to US10/526,277 priority Critical patent/US20060166201A1/en
Priority to EP03793600A priority patent/EP1534864A2/de
Priority to AU2003266197A priority patent/AU2003266197A1/en
Publication of WO2004022779A2 publication Critical patent/WO2004022779A2/de
Publication of WO2004022779A3 publication Critical patent/WO2004022779A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung beschreibt ein Verfahren zum Nachweis von Nukleinsäuresequenzen, dadurch gekennzeich-net, dass folgende Schritte ausgeführt werden: a) mindestens eine Nukleinsäure wird auf einer Festphase gebunden; b) Sondenmoleküle werden sequenzspezifisch an die Nuk-leinsäure hybridisiert, wobei die Sondenmoleküle mit ei-ner spaltbaren Bindung und einer Massenmarkierung verse-hen sind, welche für das Sondenmolekül spezifisch ist; c) Entfernung der nicht hybridisierten Sondenmoleküle; d) Kontaktieren der hybridisierten Sondenmoleküle mit ei-ner Matrix, die besagte spaltbaren Bindungen spaltet und zugleich als Matrix in einem MALDI-Massenspektrometer dient; e) Nachweis der Massenmarkierung an den Positionen, an denen die Nukleinsäure gebunden wurde.
PCT/DE2003/002936 2002-09-01 2003-09-01 Verfahren zum nachweis von nukleinsäuresequenzen mittels spaltbarer sondenmoleküle Ceased WO2004022779A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/526,277 US20060166201A1 (en) 2002-09-01 2003-09-01 Method for the detection of nucleic acid sequences by means of crackable probe molecules
EP03793600A EP1534864A2 (de) 2002-09-01 2003-09-01 Verfahren zum nachweis von nukleinsäuresequenzen mittels spaltbarer sondenmoleküle
AU2003266197A AU2003266197A1 (en) 2002-09-01 2003-09-01 Method for the detection of nucleic acid sequences by means of crackable probe molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10240746A DE10240746A1 (de) 2002-09-01 2002-09-01 Verfahren zum Nachweis von Nukleinsäuresequenzen mittels spaltbarer Sondenmoleküle
DE10240746.0 2002-09-01

Publications (2)

Publication Number Publication Date
WO2004022779A2 WO2004022779A2 (de) 2004-03-18
WO2004022779A3 true WO2004022779A3 (de) 2004-06-03

Family

ID=31724302

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2003/002936 Ceased WO2004022779A2 (de) 2002-09-01 2003-09-01 Verfahren zum nachweis von nukleinsäuresequenzen mittels spaltbarer sondenmoleküle

Country Status (5)

Country Link
US (1) US20060166201A1 (de)
EP (1) EP1534864A2 (de)
AU (1) AU2003266197A1 (de)
DE (1) DE10240746A1 (de)
WO (1) WO2004022779A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0423873D0 (en) * 2004-10-27 2004-12-01 Univ Erasmus Nucleic acid analysis
GB0811574D0 (en) * 2008-06-24 2008-07-30 Trillion Genomics Ltd Characterising planar samples by mass spectrometry
WO2010056513A2 (en) * 2008-10-30 2010-05-20 Sequenom, Inc. Products and processes for multiplex nucleic acid identification
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
WO2016172571A1 (en) 2015-04-24 2016-10-27 Agena Bioscience, Inc. Multiplexed method for the identification and quantitation of minor alleles and polymorphisms
US10640817B2 (en) 2015-04-24 2020-05-05 Agena Bioscience, Inc. Multiplex methods for detection and quantification of minor variants

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997037041A2 (en) * 1996-03-18 1997-10-09 Sequenom, Inc. Dna sequencing by mass spectrometry
WO1999060007A2 (en) * 1998-05-15 1999-11-25 Isis Innovation Limited Libraries of oligomers labelled with different tags
WO2001098527A2 (de) * 2000-06-19 2001-12-27 Epigenomics Ag Verfahren zur hochparallelen analyse von polymorphismen
DE10104938A1 (de) * 2001-01-29 2002-08-14 Epigenomics Ag Fluorescence Polarisation 1
WO2003002760A2 (de) * 2001-06-27 2003-01-09 Epigenomics Ag Verfahren zum nachweis von cytosin-methylierung durch vergleichende analyse der einzelstränge von amplifikaten

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US5952654A (en) * 1997-10-29 1999-09-14 Northeastern University Field-release mass spectrometry

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997037041A2 (en) * 1996-03-18 1997-10-09 Sequenom, Inc. Dna sequencing by mass spectrometry
WO1999060007A2 (en) * 1998-05-15 1999-11-25 Isis Innovation Limited Libraries of oligomers labelled with different tags
WO2001098527A2 (de) * 2000-06-19 2001-12-27 Epigenomics Ag Verfahren zur hochparallelen analyse von polymorphismen
DE10104938A1 (de) * 2001-01-29 2002-08-14 Epigenomics Ag Fluorescence Polarisation 1
WO2003002760A2 (de) * 2001-06-27 2003-01-09 Epigenomics Ag Verfahren zum nachweis von cytosin-methylierung durch vergleichende analyse der einzelstränge von amplifikaten

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GRIFFIN T J ET AL: "SINGLE-NUCLEOTIDE POLYMORPHISM ANALYSIS BY MALDI-TOF MASS SPECTROMETRY", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 18, 2000, pages 77 - 84, XP002952305, ISSN: 0167-7799 *
HAHNER S ET AL: "Matrix-assisted laser desorption/ionization mass spectrometry of DNA using photocleavable biotin", BIOMOLECULAR ENGINEERING, ELSEVIER, NEW YORK, NY, US, vol. 16, no. 1-4, 31 December 1999 (1999-12-31), pages 127 - 133, XP004257809, ISSN: 1389-0344 *
OLEJNIK J ET AL: "Photocleavable peptide-DNA conjugates: synthesis and applications to DNA analysis using MALDI-MS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 23, 1 December 1999 (1999-12-01), pages 4626 - 4631, XP002220753, ISSN: 0305-1048 *
SAUER SASCHA ET AL: "Facile method for automated genotyping of single nucleotide polymorphisms by mass spectrometry.", NUCLEIC ACIDS RESEARCH. ENGLAND 1 MAR 2002, vol. 30, no. 5, 1 March 2002 (2002-03-01), pages e22, XP002258710, ISSN: 1362-4962 *
SHCHEPINOV MIKHAIL S ET AL: "Matrix-induced fragmentation of P3'-N5' phosphoramidate-containing DNA: High-throughput MALDI-TOF analysis of genomic sequence polymorphisms", NUCLEIC ACIDS RESEARCH, vol. 29, no. 18, 15 September 2001 (2001-09-15), pages 3864 - 3872, XP002274768, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2004022779A2 (de) 2004-03-18
EP1534864A2 (de) 2005-06-01
US20060166201A1 (en) 2006-07-27
DE10240746A1 (de) 2004-03-18
AU2003266197A1 (en) 2004-03-29

Similar Documents

Publication Publication Date Title
WO2007014045A3 (en) Methods for rapid identification and quantitation of nucleic acid variants
WO2002072889A3 (en) Methods and compositions for microarray control
WO2007056723A3 (en) Detection of nucleic acids by target-specific hybrid capture method
WO2006007567A3 (en) Methods and compositions to detect nucleic acids in a biological sample
WO2008045136A3 (en) Nucleic acid size detection method
WO2005111235A3 (en) Methods for detecting chromosome aberrations
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
WO2004081228A3 (en) Real time gene expression profiling
WO2004092708A3 (en) Methods for the electrochemical detection of target compounds
WO2004076621A3 (en) Compositions of nucleic acids for treating and detecting influenza virus
WO2001092579A3 (en) Methods for detecting target nucleic acids using coupled ligation and amplification
WO2002042486A3 (en) Methods for detection of a nucleic acid by sequential amplification
WO2005010210A3 (en) Determining the sequence of a polynucleotide
WO2006057918A3 (en) Detection of nucleic acid variation by cleavage-amplification method
WO2009072812A3 (en) Method for selective labeling and detection of target nucleic acids using immobilized peptide nucleic acid probes
WO2009039549A3 (de) Verfahren zur bestimmung von mikroorganismen
WO2004069184A3 (en) Methods for treating, preventing and detecting helicobacter infection
WO2006117161A3 (en) New labelling strategies for the sensitive detection of analytes
JP2005538380A5 (de)
WO2004022779A3 (de) Verfahren zum nachweis von nukleinsäuresequenzen mittels spaltbarer sondenmoleküle
WO2005003318A3 (en) Assay and process for labeling and detection of micro rna and small interfering rna sequences
WO2007092279A3 (en) Detection of contaminating dna via amplification of sequences of less than 100 bp
EP1672082A3 (de) Verfahren zum Nachweis einer Zielsubstanz unter Verwendung einer Nukleinsäuresonde
WO2005042709A3 (en) Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
WO2010059914A8 (en) Nucleic acid quantification products and processes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003793600

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003793600

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2006166201

Country of ref document: US

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 10526277

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 10526277

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP