WO2004035819A3 - Oligonucleotide analogues for detecting and analyzing nucleic acids - Google Patents

Oligonucleotide analogues for detecting and analyzing nucleic acids Download PDF

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Publication number
WO2004035819A3
WO2004035819A3 PCT/DK2003/000715 DK0300715W WO2004035819A3 WO 2004035819 A3 WO2004035819 A3 WO 2004035819A3 DK 0300715 W DK0300715 W DK 0300715W WO 2004035819 A3 WO2004035819 A3 WO 2004035819A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
detecting
oligonucleotide analogues
analyzing nucleic
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DK2003/000715
Other languages
French (fr)
Other versions
WO2004035819A2 (en
WO2004035819A8 (en
Inventor
Sakari Kauppinen
Carsten Alsbo
Peter Stein Nielsen
Daniel Charlton Jeffares
Tobias Mourier
Soeren Moerk
Peter Arctander
Niels Tommerup
Niels Tolstrup
Henrik Vissing
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exiqon AS
Original Assignee
Exiqon AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon AS filed Critical Exiqon AS
Priority to AU2003273778A priority Critical patent/AU2003273778A1/en
Priority to EP03757735A priority patent/EP1556510A2/en
Publication of WO2004035819A2 publication Critical patent/WO2004035819A2/en
Publication of WO2004035819A3 publication Critical patent/WO2004035819A3/en
Publication of WO2004035819A8 publication Critical patent/WO2004035819A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention features improved non-naturally occurring nucleic acids with higher melting temperature than control nucleic acid with 2’ deoxynukleotides, and methods for expression profiling of mRNAs, identifying and profiling of particular mRNA splice variants, and detecting mutations, deletions, or duplications of particular exons or other splice variants, e.g., alterations associated with a disease such as cancer, in a nucleic acid sample, e.g., a biological sample or a patient sample.
PCT/DK2003/000715 2002-10-21 2003-10-21 Oligonucleotide analogues for detecting and analyzing nucleic acids Ceased WO2004035819A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003273778A AU2003273778A1 (en) 2002-10-21 2003-10-21 Oligonucleotide analogues for detecting and analyzing nucleic acids
EP03757735A EP1556510A2 (en) 2002-10-21 2003-10-21 Oligonucleotide analogues for detecting and analyzing nucleic acids

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US42027802P 2002-10-21 2002-10-21
US60/420,278 2002-10-21
DKPA200300752 2003-05-19
DKPA200300752 2003-05-19

Publications (3)

Publication Number Publication Date
WO2004035819A2 WO2004035819A2 (en) 2004-04-29
WO2004035819A3 true WO2004035819A3 (en) 2004-08-26
WO2004035819A8 WO2004035819A8 (en) 2004-09-30

Family

ID=32109043

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000715 Ceased WO2004035819A2 (en) 2002-10-21 2003-10-21 Oligonucleotide analogues for detecting and analyzing nucleic acids

Country Status (3)

Country Link
EP (1) EP1556510A2 (en)
AU (1) AU2003273778A1 (en)
WO (1) WO2004035819A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106068329A (en) * 2014-01-08 2016-11-02 基诺泰科有限公司 Utilize 5 ' the repressed archaeal dna polymerases of flap endonuclease activity the method detecting mutant gene by real-time polymerase chain reaction

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8232055B2 (en) 2002-12-23 2012-07-31 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide features and compositions for practicing the same
US8321138B2 (en) 2005-07-29 2012-11-27 Agilent Technologies, Inc. Method of characterizing quality of hybridized CGH arrays
WO2006076025A2 (en) * 2004-05-14 2006-07-20 Amaox, Inc. Immune cell biosensors and methods of using same
JP2008505649A (en) * 2004-07-09 2008-02-28 アモークス リミテッド Immune cell biosensor and method of use thereof
WO2008111908A1 (en) * 2007-03-15 2008-09-18 Jyoti Chattopadhyaya Five- and six-membered conformationally locked 2',4'- carbocyclic ribo-thymidines for the treatment of infections and cancer
DK2548962T3 (en) 2007-09-19 2016-04-11 Applied Biosystems Llc Sirna sequence-independent modification formats to reduce off-target phenotype effects in RNAI and stabilized forms thereof
US20140031250A1 (en) 2010-10-07 2014-01-30 David Tsai Ting Biomarkers of Cancer
US8273235B2 (en) 2010-11-05 2012-09-25 Roshan V Chapaneri Dark colored chromium based electrodeposits
US10301624B2 (en) 2014-06-25 2019-05-28 The General Hospital Corporation Targeting human satellite II (HSATII)
JP7138349B2 (en) 2017-02-21 2022-09-16 国立大学法人大阪大学 Nucleic acid compounds and oligonucleotides
CN112041439A (en) 2018-02-14 2020-12-04 深度基因组学公司 Oligonucleotide therapy for Wilson's disease
CN121674413A (en) * 2026-02-11 2026-03-17 中国科学院海洋研究所 Material for regulating and controlling carotenoid enrichment in crustacean body and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014226A2 (en) * 1997-09-12 1999-03-25 Exiqon A/S Bi- and tri-cyclic nucleoside, nucleotide and oligonucleotide analogues
WO2001081632A1 (en) * 2000-04-25 2001-11-01 Affymetrix, Inc. Methods for monitoring the expression of alternatively spliced genes
WO2002061387A2 (en) * 2000-10-25 2002-08-08 Exiqon A/S Open substrate platforms suitable for analysis of biomolecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999014226A2 (en) * 1997-09-12 1999-03-25 Exiqon A/S Bi- and tri-cyclic nucleoside, nucleotide and oligonucleotide analogues
WO2001081632A1 (en) * 2000-04-25 2001-11-01 Affymetrix, Inc. Methods for monitoring the expression of alternatively spliced genes
WO2002061387A2 (en) * 2000-10-25 2002-08-08 Exiqon A/S Open substrate platforms suitable for analysis of biomolecules

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DWAINE A. BRAASCH ET AL: "Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA", CHEMISTRY & BIOLOGY, vol. 8, 2001, pages 1 - 7, XP002285000 *
NIELS TOLSTRUP ET AL: "OligoDesign: optimal design of LNA (locked nucleic acid) oligonucleotide capture probes for gene expression profiling", NUCLEIC ACIDS RESEARCH, vol. 31, no. 13, 2003, pages 3758 - 3762, XP002285001 *
See also references of EP1556510A2 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106068329A (en) * 2014-01-08 2016-11-02 基诺泰科有限公司 Utilize 5 ' the repressed archaeal dna polymerases of flap endonuclease activity the method detecting mutant gene by real-time polymerase chain reaction
CN106068329B (en) * 2014-01-08 2020-03-27 基诺泰科有限公司 Method for detecting mutant gene by real-time polymerase chain reaction

Also Published As

Publication number Publication date
WO2004035819A2 (en) 2004-04-29
AU2003273778A1 (en) 2004-05-04
AU2003273778A8 (en) 2004-05-04
WO2004035819A8 (en) 2004-09-30
EP1556510A2 (en) 2005-07-27

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