WO2004052606A3 - Detection, identification and differentiation of eubacterial taxa using a hybridization assay - Google Patents

Detection, identification and differentiation of eubacterial taxa using a hybridization assay Download PDF

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Publication number
WO2004052606A3
WO2004052606A3 PCT/EP2003/013907 EP0313907W WO2004052606A3 WO 2004052606 A3 WO2004052606 A3 WO 2004052606A3 EP 0313907 W EP0313907 W EP 0313907W WO 2004052606 A3 WO2004052606 A3 WO 2004052606A3
Authority
WO
WIPO (PCT)
Prior art keywords
identification
detection
differentiation
nucleic acid
hybridization assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/013907
Other languages
French (fr)
Other versions
WO2004052606A2 (en
Inventor
Sofie Claeys
Geert Jannes
Gerd Haberhausen
Thomas Emrich
Lia Verdoodt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Europe NV SA
Roche Diagnostics GmbH
Original Assignee
Innogenetics NV SA
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AU2003289984A priority Critical patent/AU2003289984A1/en
Priority to US10/537,787 priority patent/US20060115819A1/en
Priority to JP2004558030A priority patent/JP2006508671A/en
Priority to DE60327462T priority patent/DE60327462D1/en
Priority to EP03782334A priority patent/EP1567668B1/en
Priority to HK06102673.4A priority patent/HK1085159B/en
Priority to DK03782334T priority patent/DK1567668T3/en
Priority to CA2504911A priority patent/CA2504911C/en
Application filed by Innogenetics NV SA, Roche Diagnostics GmbH filed Critical Innogenetics NV SA
Priority to AT03782334T priority patent/ATE430209T1/en
Publication of WO2004052606A2 publication Critical patent/WO2004052606A2/en
Publication of WO2004052606A3 publication Critical patent/WO2004052606A3/en
Anticipated expiration legal-status Critical
Priority to US12/105,380 priority patent/US8945850B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a method for the specific detection and/or identification of Staphylococcus species, in particular Staphylococcus aureus, using new nucleic acid sequences derived from the ITS (Internal Transcribed Spacer) region.The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Staphylococcus species, in particular of S. aureus, in a biological sample.It relates also to nucleic acid primers to be used for the amplification of said spacer region of Staphylococcus species in a sample.
PCT/EP2003/013907 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay Ceased WO2004052606A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
DK03782334T DK1567668T3 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
JP2004558030A JP2006508671A (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial populations using hybridization assays
DE60327462T DE60327462D1 (en) 2002-12-06 2003-12-08 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EU BACTERIA USING A HYBRIDIZATION ASSAY
EP03782334A EP1567668B1 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
HK06102673.4A HK1085159B (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
AU2003289984A AU2003289984A1 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
US10/537,787 US20060115819A1 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
CA2504911A CA2504911C (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay
AT03782334T ATE430209T1 (en) 2002-12-06 2003-12-08 DETECTION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIA USING A HYBRIDIZATION ASSAY
US12/105,380 US8945850B2 (en) 2002-12-06 2008-04-18 Detection, identification and differentiation of Staphylococcus using a hybridization assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02447247 2002-12-06
EP02447247.4 2002-12-06

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10537787 A-371-Of-International 2003-12-08
US12/105,380 Continuation US8945850B2 (en) 2002-12-06 2008-04-18 Detection, identification and differentiation of Staphylococcus using a hybridization assay

Publications (2)

Publication Number Publication Date
WO2004052606A2 WO2004052606A2 (en) 2004-06-24
WO2004052606A3 true WO2004052606A3 (en) 2004-07-29

Family

ID=32479868

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/013907 Ceased WO2004052606A2 (en) 2002-12-06 2003-12-08 Detection, identification and differentiation of eubacterial taxa using a hybridization assay

Country Status (10)

Country Link
US (2) US20060115819A1 (en)
EP (1) EP1567668B1 (en)
JP (2) JP2006508671A (en)
AT (1) ATE430209T1 (en)
AU (1) AU2003289984A1 (en)
CA (1) CA2504911C (en)
DE (1) DE60327462D1 (en)
DK (1) DK1567668T3 (en)
ES (1) ES2326316T3 (en)
WO (1) WO2004052606A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003289984A1 (en) * 2002-12-06 2004-06-30 Innogenetics N.V. Detection, identification and differentiation of eubacterial taxa using a hybridization assay
US7865739B2 (en) * 2003-09-02 2011-01-04 Sony Ericsson Mobile Communications Ab Methods and devices for transferring security data between memories
US20060099618A1 (en) * 2004-09-30 2006-05-11 Innogenetics, N.V. Detection, identification and differentiation of Serratia species using the spacer region
JP4967095B2 (en) * 2005-08-12 2012-07-04 財団法人ヒューマンサイエンス振興財団 Method for measuring the concentration of Staphylococcus aureus and method for determining the possibility of food poisoning
EP1977001A4 (en) * 2006-01-20 2009-12-02 Genein Co Ltd Oligonucleotide for detection of bacteria associated with sepsis and microarrays and a methods for detection of the bacteria using the oligonucleotide
WO2008084889A1 (en) * 2007-01-08 2008-07-17 Medigenes Co., Ltd Dna chip for detection of staphylococcus aureus
WO2008129428A2 (en) 2007-04-19 2008-10-30 Molecular Detection Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0786519A2 (en) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences
US5849488A (en) * 1996-02-27 1998-12-15 Oulutech Ltd. DNA-sequence-based diagnosis of mastitis from a milk sample
US6312903B1 (en) * 1994-06-24 2001-11-06 Innogenetics N.V. Simulataneous detection, identification and differentiation of eubacterial taxa using a hybridization assay

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP3844996B2 (en) * 2000-11-15 2006-11-15 エフ．ホフマン−ラ ロシュ アーゲー Melting curve analysis method for repetitive PCR products
AU2003289984A1 (en) 2002-12-06 2004-06-30 Innogenetics N.V. Detection, identification and differentiation of eubacterial taxa using a hybridization assay

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312903B1 (en) * 1994-06-24 2001-11-06 Innogenetics N.V. Simulataneous detection, identification and differentiation of eubacterial taxa using a hybridization assay
EP0786519A2 (en) * 1996-01-05 1997-07-30 Human Genome Sciences, Inc. Staphylococcus aureus polynucleotides and sequences
US5849488A (en) * 1996-02-27 1998-12-15 Oulutech Ltd. DNA-sequence-based diagnosis of mastitis from a milk sample

Also Published As

Publication number Publication date
DK1567668T3 (en) 2009-08-17
JP5156726B2 (en) 2013-03-06
JP2006508671A (en) 2006-03-16
DE60327462D1 (en) 2009-06-10
HK1085159A1 (en) 2006-08-18
US20060115819A1 (en) 2006-06-01
ES2326316T3 (en) 2009-10-07
EP1567668B1 (en) 2009-04-29
ATE430209T1 (en) 2009-05-15
WO2004052606A2 (en) 2004-06-24
CA2504911C (en) 2015-08-11
AU2003289984A8 (en) 2004-06-30
AU2003289984A1 (en) 2004-06-30
US20080199878A1 (en) 2008-08-21
JP2010088447A (en) 2010-04-22
CA2504911A1 (en) 2004-06-24
EP1567668A2 (en) 2005-08-31
US8945850B2 (en) 2015-02-03

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