WO2004058391A3 - Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren - Google Patents

Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren Download PDF

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Publication number
WO2004058391A3
WO2004058391A3 PCT/EP2003/014822 EP0314822W WO2004058391A3 WO 2004058391 A3 WO2004058391 A3 WO 2004058391A3 EP 0314822 W EP0314822 W EP 0314822W WO 2004058391 A3 WO2004058391 A3 WO 2004058391A3
Authority
WO
WIPO (PCT)
Prior art keywords
protective groups
synthesis
stage protective
photoactivatable
biopolymers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2003/014822
Other languages
English (en)
French (fr)
Other versions
WO2004058391A2 (de
Inventor
Barbro Beijer
Ramon Gueimil
Matthias Scheffler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Febit AG
Original Assignee
Febit AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10260592A external-priority patent/DE10260592A1/de
Priority claimed from DE10260591A external-priority patent/DE10260591A1/de
Application filed by Febit AG filed Critical Febit AG
Priority to AU2003290117A priority Critical patent/AU2003290117A1/en
Priority to EP03782479A priority patent/EP1585830A2/de
Priority to US10/540,396 priority patent/US7737089B2/en
Publication of WO2004058391A2 publication Critical patent/WO2004058391A2/de
Publication of WO2004058391A3 publication Critical patent/WO2004058391A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nanotechnology (AREA)
  • Composite Materials (AREA)
  • General Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Structural Engineering (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Indole Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Synthese von Biopolymeren durch schrittweisen Aufbau aus geschützten Synthesebausteinen, die zweistufige Schutzgruppen tragen. Die zweistufigen Schutzgruppen werden durch einen ersten Belichtungsschritt aktiviert und einen anschliessenden chemischen Behandlungsschritt abgespalten. Es werden photoaktivierbare Komponenten verwendet, die über intramolekulare Triplettsensibilisatoren den Aktivierungsprozess erheblich beschleunigen oder/und über Fluoreszenzeigenschaften verfügen. Die zweistufigen Schutzgruppen können insbesondere im Rahmen einer Qualitätskontrolle Anwendung finden.
PCT/EP2003/014822 2002-12-23 2003-12-23 Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren Ceased WO2004058391A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003290117A AU2003290117A1 (en) 2002-12-23 2003-12-23 Photoactivatable two-stage protective groups for the synthesis of biopolymers
EP03782479A EP1585830A2 (de) 2002-12-23 2003-12-23 Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren
US10/540,396 US7737089B2 (en) 2002-12-23 2003-12-23 Photoactivatable two-stage protective groups for the synthesis of biopolymers

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10260592.0 2002-12-23
DE10260592A DE10260592A1 (de) 2002-12-23 2002-12-23 Intramolekular triplettsensibilisierte o-Nitrophenylethyl-Photoschutzgruppen
DE10260591A DE10260591A1 (de) 2002-12-23 2002-12-23 Photoaktivierbare zweistufige Schutzgruppen für die Synthese von Biopolymeren
DE10260591.2 2002-12-23

Publications (2)

Publication Number Publication Date
WO2004058391A2 WO2004058391A2 (de) 2004-07-15
WO2004058391A3 true WO2004058391A3 (de) 2004-08-26

Family

ID=32683475

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2003/014823 Ceased WO2004058392A2 (de) 2002-12-23 2003-12-23 Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen
PCT/EP2003/014822 Ceased WO2004058391A2 (de) 2002-12-23 2003-12-23 Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/014823 Ceased WO2004058392A2 (de) 2002-12-23 2003-12-23 Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen

Country Status (4)

Country Link
US (1) US7737089B2 (de)
EP (1) EP1585830A2 (de)
AU (2) AU2003298235A1 (de)
WO (2) WO2004058392A2 (de)

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WO2004058392A2 (de) * 2002-12-23 2004-07-15 Febit Ag Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen
DE102005027667A1 (de) * 2005-06-15 2006-12-28 Febit Biotech Gmbh Verfahren zur Qualitätskontrolle funktionalisierter Oberflächen
TWI707038B (zh) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
KR102514213B1 (ko) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 면역 시냅스의 변이체 라이브러리 및 그의 합성
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Ｃｒｔｈ２のバリアント核酸ライブラリー
IL293670A (en) 2019-12-09 2022-08-01 Twist Bioscience Corp Variable nucleic acid libraries for adenosine receptors

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066259A1 (en) * 1999-05-05 2000-11-09 Ut-Battelle, Llc Method and apparatus for combinatorial chemistry
WO2002020150A2 (en) * 2000-09-11 2002-03-14 Affymetrix, Inc. Photocleavable protecting groups
WO2002062815A1 (de) * 2001-02-05 2002-08-15 Febit Ag Hybrid-schutzgruppe
DE10122357A1 (de) * 2001-05-09 2002-11-14 Febit Ferrarius Biotech Gmbh Hybridverfahren zur Herstellung von Trägern für die Analytbestimmung
WO2003004510A1 (de) * 2001-07-03 2003-01-16 Febit Ag Zweistufige schutzgruppen für die synthese von biopolymeren

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DE4444996A1 (de) * 1994-12-16 1996-06-20 Wolfgang Prof Dr Dr Pfleiderer Nucleosid-Derivate mit photolabilen Schutzgruppen
DE19620170A1 (de) * 1996-05-20 1997-11-27 Wolfgang Prof Dr Dr Pfleiderer Nucleosid-Derivate mit photolabilen Schutzgruppen
AU5059800A (en) * 1999-04-08 2000-11-14 Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts Nucleoside derivatives with photo-unstable protective groups
DE10105079A1 (de) * 2001-02-05 2002-08-08 Febit Ferrarius Biotech Gmbh Fotolabile Schutzgruppen für die Synthese von Biopolymeren
WO2004058392A2 (de) * 2002-12-23 2004-07-15 Febit Ag Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066259A1 (en) * 1999-05-05 2000-11-09 Ut-Battelle, Llc Method and apparatus for combinatorial chemistry
WO2002020150A2 (en) * 2000-09-11 2002-03-14 Affymetrix, Inc. Photocleavable protecting groups
WO2002062815A1 (de) * 2001-02-05 2002-08-15 Febit Ag Hybrid-schutzgruppe
DE10122357A1 (de) * 2001-05-09 2002-11-14 Febit Ferrarius Biotech Gmbh Hybridverfahren zur Herstellung von Trägern für die Analytbestimmung
WO2003004510A1 (de) * 2001-07-03 2003-01-16 Febit Ag Zweistufige schutzgruppen für die synthese von biopolymeren

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAPP E ET AL: "NEW TRITYL-BASED PROTECTING GROUPS WITH A MILD TWO-STEP REMOVAL", NUCLEOSIDES & NUCLEOTIDES, DEKKER, NEW YORK,NY,, US, vol. 7, no. 5/6, 1988, pages 813 - 816, XP008011020, ISSN: 0732-8311 *

Also Published As

Publication number Publication date
US20060111564A1 (en) 2006-05-25
AU2003298235A1 (en) 2004-07-22
WO2004058392A3 (de) 2004-08-26
WO2004058392A2 (de) 2004-07-15
AU2003290117A1 (en) 2004-07-22
US7737089B2 (en) 2010-06-15
AU2003290117A8 (en) 2004-07-22
EP1585830A2 (de) 2005-10-19
AU2003298235A8 (en) 2004-07-22
WO2004058391A2 (de) 2004-07-15

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