WO2004065573A3 - Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis - Google Patents

Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis Download PDF

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Publication number
WO2004065573A3
WO2004065573A3 PCT/US2004/000538 US2004000538W WO2004065573A3 WO 2004065573 A3 WO2004065573 A3 WO 2004065573A3 US 2004000538 W US2004000538 W US 2004000538W WO 2004065573 A3 WO2004065573 A3 WO 2004065573A3
Authority
WO
WIPO (PCT)
Prior art keywords
generating
gene expression
high throughput
expression analysis
novel high
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/000538
Other languages
French (fr)
Other versions
WO2004065573A2 (en
Inventor
Joseph Peter Luciano Jr
Eugene L Brown
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth LLC
Original Assignee
Wyeth LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth LLC filed Critical Wyeth LLC
Priority to EP04701455A priority Critical patent/EP1583831A2/en
Priority to CA002509512A priority patent/CA2509512A1/en
Priority to AU2004206206A priority patent/AU2004206206A1/en
Publication of WO2004065573A2 publication Critical patent/WO2004065573A2/en
Publication of WO2004065573A3 publication Critical patent/WO2004065573A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of generating substantially pure polynucleotides in a multiple-compartment container using a multiple-compartment purification filter is described. In particular, a method of generating cDNA or cRNA labeled with biotin for the ease of detection is described.
PCT/US2004/000538 2003-01-15 2004-01-12 Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis Ceased WO2004065573A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP04701455A EP1583831A2 (en) 2003-01-15 2004-01-12 Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis
CA002509512A CA2509512A1 (en) 2003-01-15 2004-01-12 Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis
AU2004206206A AU2004206206A1 (en) 2003-01-15 2004-01-12 Novel high throughput method of generating and purifying labeled cRNA targets for gene expression analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34240103A 2003-01-15 2003-01-15
US10/342,401 2003-01-15

Publications (2)

Publication Number Publication Date
WO2004065573A2 WO2004065573A2 (en) 2004-08-05
WO2004065573A3 true WO2004065573A3 (en) 2005-03-31

Family

ID=32770201

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/000538 Ceased WO2004065573A2 (en) 2003-01-15 2004-01-12 Novel high throughput method of generating and purifying labeled crna targets for gene expression analysis

Country Status (4)

Country Link
EP (1) EP1583831A2 (en)
AU (1) AU2004206206A1 (en)
CA (1) CA2509512A1 (en)
WO (1) WO2004065573A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005003375A2 (en) * 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
EP1930422B1 (en) * 2005-08-19 2013-01-23 Sumitomo Bakelite Company, Ltd. Method for producing cdna and rna chains and nucleotide-immobilized support
ES2358473T3 (en) 2007-05-11 2011-05-11 Decathlon ARTICLE OF CONFECTION WITH EFFECT OF HETEROGENIC CONTAINMENT FOR THE PRACTICE OF A SPORT.

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002002741A2 (en) * 2000-07-05 2002-01-10 Rosetta Inpharmatics, Inc. Methods for genetic interpretation and prediction of phenotype

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002002741A2 (en) * 2000-07-05 2002-01-10 Rosetta Inpharmatics, Inc. Methods for genetic interpretation and prediction of phenotype

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Protocol for Amino-aallyl Reverse Transcription and NHS-Cy Dye Labeling", INTERNET ARTICLE, 18 May 2002 (2002-05-18), XP002288467, Retrieved from the Internet <URL:http://web.archive.org/web/20020618004616/ubik.microbiol.washington.edu/protocols/arrays/aminoallylprobe.htm> [retrieved on 20040810] *
JEREMY HASSEMAN: "Aminoallyl labeling of RNA for microarrays", INTERNET ARTICLE, 3 April 2002 (2002-04-03), pages 1 - 8, XP002288466, Retrieved from the Internet <URL:http://web.archive.org/web/20040713090708/http://pga.tigr.org/sop/M004_1a.pdf> [retrieved on 20040810] *
QIAGEN: "Qiaquick Multiwell PCR Purification Handbook", March 2001, QIAGEN, XP002288468 *

Also Published As

Publication number Publication date
CA2509512A1 (en) 2004-08-05
AU2004206206A1 (en) 2004-08-05
EP1583831A2 (en) 2005-10-12
WO2004065573A2 (en) 2004-08-05

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