WO2004092214A3 - Affinity purification system using troponin molecules as affinity ligands - Google Patents

Affinity purification system using troponin molecules as affinity ligands Download PDF

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Publication number
WO2004092214A3
WO2004092214A3 PCT/US2004/010887 US2004010887W WO2004092214A3 WO 2004092214 A3 WO2004092214 A3 WO 2004092214A3 US 2004010887 W US2004010887 W US 2004010887W WO 2004092214 A3 WO2004092214 A3 WO 2004092214A3
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WO
WIPO (PCT)
Prior art keywords
troponin
affinity
molecules
methods
purification system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/010887
Other languages
French (fr)
Other versions
WO2004092214A2 (en
Inventor
Randolph A Moeckli
Christopher C Chadwick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ARISTEX
Original Assignee
ARISTEX
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ARISTEX filed Critical ARISTEX
Priority to EP04759297A priority Critical patent/EP1618199A2/en
Publication of WO2004092214A2 publication Critical patent/WO2004092214A2/en
Publication of WO2004092214A3 publication Critical patent/WO2004092214A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4716—Muscle proteins, e.g. myosin, actin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention pertains to the use of a novel set of tags for the immobilization and/or purification of proteins or other biological or organic molecules. The invention provides troponin C, troponin C binding peptide, and troponin I, or active fragments or analogues thereof as convenient tags and affinity ligands for immobilizing, attaching, or purifying proteins or other molecules. Methods for producing troponin-tagged molecules, such as recombinant fusion proteins, are described. Methods for preparing a troponin affinity matrix that is capable of specifically binding its cognate ligand in the presence of calcium, and methods for using such a matrix to purify troponin-tagged molecules are also described.
PCT/US2004/010887 2003-04-10 2004-04-07 Affinity purification system using troponin molecules as affinity ligands Ceased WO2004092214A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04759297A EP1618199A2 (en) 2003-04-10 2004-04-07 Affinity purification system using troponin molecules as affinity ligands

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46248303P 2003-04-10 2003-04-10
US60/462,483 2003-04-10

Publications (2)

Publication Number Publication Date
WO2004092214A2 WO2004092214A2 (en) 2004-10-28
WO2004092214A3 true WO2004092214A3 (en) 2004-11-18

Family

ID=33299948

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/010887 Ceased WO2004092214A2 (en) 2003-04-10 2004-04-07 Affinity purification system using troponin molecules as affinity ligands

Country Status (3)

Country Link
US (2) US20050043510A1 (en)
EP (1) EP1618199A2 (en)
WO (1) WO2004092214A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100173367A1 (en) * 2006-10-27 2010-07-08 Marner Ii Wesley D Functionalized molecules comprising an autosilification moiety and methods of making and using same
WO2012028697A1 (en) 2010-09-01 2012-03-08 Eth Zürich, Institute Of Molecular Biology And Biophysics Affinity purification system based on donor strand complementation
US20200236971A1 (en) * 2019-01-29 2020-07-30 Bond Pet Foods, Inc. Compositions and methods for producing recombinant animal proteins in prokaryotic organisms for use in food and feed

Citations (1)

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Publication number Priority date Publication date Assignee Title
JP2002058479A (en) * 2000-08-14 2002-02-26 Canon Inc Method for obtaining structure-recognition amino acid sequence

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US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US4671958A (en) * 1982-03-09 1987-06-09 Cytogen Corporation Antibody conjugates for the delivery of compounds to target sites
US4625014A (en) * 1984-07-10 1986-11-25 Dana-Farber Cancer Institute, Inc. Cell-delivery agent
US4542225A (en) * 1984-08-29 1985-09-17 Dana-Farber Cancer Institute, Inc. Acid-cleavable compound
US4659839A (en) * 1984-10-10 1987-04-21 Mallinckrodt, Inc. Coupling agents for radiolabeled antibody fragments
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5166320A (en) * 1987-04-22 1992-11-24 University Of Connecticut Carrier system and method for the introduction of genes into mammalian cells
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5386023A (en) * 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) * 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
CA2130280C (en) * 1993-08-24 1999-08-31 Lilian Lee Method for purification of cardiac troponin i
US6117976A (en) * 1993-11-04 2000-09-12 Medical Research Council Manufacture and use of polypeptides tagged using binding molecules
US5637684A (en) * 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
DE19524572A1 (en) * 1995-07-06 1997-01-09 Bayer Ag Method for producing a synthetic calibrator for use in immunoassays, consisting of the analytes or partial sequences thereof, which are conjugated to inert carrier molecules
GB9608510D0 (en) * 1996-04-25 1996-07-03 Medical Res Council Calcium dependent binding ligands
GB9608540D0 (en) * 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
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US6475785B1 (en) * 1997-12-18 2002-11-05 Spectral Diagnostics, Inc. Single-chain polypeptides comprising troponin I N-terminal fragments and troponin C
WO2000023585A1 (en) * 1998-10-21 2000-04-27 Spectral Diagnostics, Inc. Cardiac troponin i polypeptide fragments and uses in diagnostics
AU2001278968A1 (en) * 2000-07-19 2002-01-30 Pointilliste, Inc. Nested sorting and high throughput screening

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002058479A (en) * 2000-08-14 2002-02-26 Canon Inc Method for obtaining structure-recognition amino acid sequence

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
MORJANA N ET AL: "Expression and equilibrium denaturation of cardiac troponin I: stabilization of a folding intermediate during denaturation by urea.", BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. AUG 1998, vol. 28 ( Pt 1), August 1998 (1998-08-01), pages 7 - 17, XP002298413, ISSN: 0885-4513 *
PATENT ABSTRACTS OF JAPAN vol. 2002, no. 06 4 June 2002 (2002-06-04) *
PIERCE H H ET AL: "Identification of troponin C antagonists from a phage-displayed random peptide library.", THE JOURNAL OF BIOLOGICAL CHEMISTRY. 4 SEP 1998, vol. 273, no. 36, 4 September 1998 (1998-09-04), pages 23448 - 23453, XP002298416, ISSN: 0021-9258 *
SYSKA H ET AL: "A NEW METHOD OF PREPARATION OF TROPONIN I INHIBITORY PROTEIN USING AFFINITY CHROMATOGRAPHY EVIDENCE FOR 3 DIFFERENT FORMS OF TROPONIN I IN STRIATED MUSCLE", FEBS LETTERS, vol. 40, no. 2, 1974, pages 253 - 257, XP002298414, ISSN: 0014-5793 *
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WANG C-L A ET AL: "BINDING OF LANTHANIDE IONS TO TROPONIN C", BIOCHEMISTRY, vol. 20, no. 9, 1981, pages 2439 - 2444, XP008036132, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
US20060155118A1 (en) 2006-07-13
WO2004092214A2 (en) 2004-10-28
EP1618199A2 (en) 2006-01-25
US20050043510A1 (en) 2005-02-24

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