WO2005026368A3 - Dihydroflavonol-4-reductase - Google Patents

Dihydroflavonol-4-reductase Download PDF

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Publication number
WO2005026368A3
WO2005026368A3 PCT/US2004/029541 US2004029541W WO2005026368A3 WO 2005026368 A3 WO2005026368 A3 WO 2005026368A3 US 2004029541 W US2004029541 W US 2004029541W WO 2005026368 A3 WO2005026368 A3 WO 2005026368A3
Authority
WO
WIPO (PCT)
Prior art keywords
dihydroflavonol
reductase
recombinant dna
dna construct
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/029541
Other languages
French (fr)
Other versions
WO2005026368A2 (en
Inventor
Gary M Fader
Omolayo O Famodu
Lisa L Huang
Alexandre Da Silva Conceicao
Brian Mcgonigle
Yong Tao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to CA002538314A priority Critical patent/CA2538314A1/en
Priority to EP04783682A priority patent/EP1668138A2/en
Publication of WO2005026368A2 publication Critical patent/WO2005026368A2/en
Publication of WO2005026368A3 publication Critical patent/WO2005026368A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a dihydroflavonol-4-reductase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of dihydroflavonol-4-reductase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the dihydroflavonol-4-reductase in a transformed host cell.
PCT/US2004/029541 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase Ceased WO2005026368A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002538314A CA2538314A1 (en) 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase
EP04783682A EP1668138A2 (en) 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50178803P 2003-09-10 2003-09-10
US60/501,788 2003-09-10

Publications (2)

Publication Number Publication Date
WO2005026368A2 WO2005026368A2 (en) 2005-03-24
WO2005026368A3 true WO2005026368A3 (en) 2005-06-02

Family

ID=34312307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/029541 Ceased WO2005026368A2 (en) 2003-09-10 2004-09-09 Dihydroflavonol-4-reductase

Country Status (3)

Country Link
EP (1) EP1668138A2 (en)
CA (1) CA2538314A1 (en)
WO (1) WO2005026368A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113755460B (en) * 2021-09-10 2023-04-14 浙江华睿生物技术有限公司 Flavone reductase for preparing dihydroquercetin

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996036716A1 (en) * 1995-05-16 1996-11-21 International Flower Developments Pty. Ltd. Transgenic plants exhibiting altered flower colour and methods for producing same
EP1182257A1 (en) * 2000-08-14 2002-02-27 Korea Kumho Petrochemical Co. Ltd. Nucleic acids encoding dihydroflavonol 4-reductase with altered specificity and uses thereof
WO2002101023A2 (en) * 2001-06-13 2002-12-19 E.I. Du Pont De Nemours And Company A method for altering the isoflavonoid profile in the plant parts of an isoflavonoid-producing plant
WO2003031622A1 (en) * 2001-10-05 2003-04-17 Agriculture Victoria Services Pty Ltd Manipulation of flavonoid biosynthesis in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996036716A1 (en) * 1995-05-16 1996-11-21 International Flower Developments Pty. Ltd. Transgenic plants exhibiting altered flower colour and methods for producing same
EP1182257A1 (en) * 2000-08-14 2002-02-27 Korea Kumho Petrochemical Co. Ltd. Nucleic acids encoding dihydroflavonol 4-reductase with altered specificity and uses thereof
WO2002101023A2 (en) * 2001-06-13 2002-12-19 E.I. Du Pont De Nemours And Company A method for altering the isoflavonoid profile in the plant parts of an isoflavonoid-producing plant
WO2003031622A1 (en) * 2001-10-05 2003-04-17 Agriculture Victoria Services Pty Ltd Manipulation of flavonoid biosynthesis in plants

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 10 September 1999 (1999-09-10), "Glycine max dihydroflavonol-4-reductase DFR1 mRNA, complete cds.", XP002311362, retrieved from EBI accession no. EM_PRO:AF167556 Database accession no. AF167556 *
MEYER P ET AL: "A NEW PETUNIA FLOWER COLOR GENERATED BY TRANSFORMATION OF A MUTANT WITH A MAIZE GENE", NATURE (LONDON), vol. 330, no. 6149, 1987, pages 677 - 678, XP002311408, ISSN: 0028-0836 *
SPARVOLI F ET AL: "CLONING AND MOLECULAR ANALYSIS OF STRUCTURAL GENES INVOLVED IN FLAVONOID AND STILBENE BIOSYNTHESIS IN GRAPE (VITIS VINIFERA L.)", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 24, no. 5, 1994, pages 743 - 755, XP001161123, ISSN: 0167-4412 *
TANAKA Y ET AL: "MOLECULAR CLONING AND CHARACTERIZATION OF ROSA HYBRIDA DIHYDROFLAVONOL 4-REDUCTASE GENE", PLANT AND CELL PHYSIOLOGY, JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS, XX, vol. 36, no. 6, September 1995 (1995-09-01), pages 1023 - 1031, XP001079999, ISSN: 0032-0781 *

Also Published As

Publication number Publication date
EP1668138A2 (en) 2006-06-14
WO2005026368A2 (en) 2005-03-24
CA2538314A1 (en) 2005-03-24

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