WO2005030983A3 - Amplification of polynucleotides by rolling circle amplification - Google Patents

Amplification of polynucleotides by rolling circle amplification Download PDF

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Publication number
WO2005030983A3
WO2005030983A3 PCT/US2004/031652 US2004031652W WO2005030983A3 WO 2005030983 A3 WO2005030983 A3 WO 2005030983A3 US 2004031652 W US2004031652 W US 2004031652W WO 2005030983 A3 WO2005030983 A3 WO 2005030983A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
amplification
acid molecule
rolling circle
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/031652
Other languages
French (fr)
Other versions
WO2005030983A2 (en
Inventor
Youxiang Wang
Yaping Zong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CIRCLEAMP Inc
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CIRCLEAMP Inc
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Filing date
Publication date
Application filed by CIRCLEAMP Inc filed Critical CIRCLEAMP Inc
Priority to CA002540087A priority Critical patent/CA2540087A1/en
Priority to EP04785129A priority patent/EP1664328A2/en
Publication of WO2005030983A2 publication Critical patent/WO2005030983A2/en
Anticipated expiration legal-status Critical
Publication of WO2005030983A3 publication Critical patent/WO2005030983A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Rolling circle amplification is used to amplify and detect target nucleic acid molecules by affixing a first and/or second linker nucleic acid molecule or a second linker nucleic acid molecule to the target nucleic acid molecule, then circularizing the target nucleic acid molecule, and then amplifying the circularized nucleic acid molecule to generate reiterated nucleic acid sequences. The methods may be used to amplify nucleic acids, particularly RNA, for detection and cloning.
PCT/US2004/031652 2003-09-26 2004-09-27 Amplification of polynucleotides by rolling circle amplification Ceased WO2005030983A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002540087A CA2540087A1 (en) 2003-09-26 2004-09-27 Amplification of polynucleotides by rolling circle amplification
EP04785129A EP1664328A2 (en) 2003-09-26 2004-09-27 Amplification of polynucleotides by rolling circle amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50621803P 2003-09-26 2003-09-26
US60/506,218 2003-09-26

Publications (2)

Publication Number Publication Date
WO2005030983A2 WO2005030983A2 (en) 2005-04-07
WO2005030983A3 true WO2005030983A3 (en) 2009-04-16

Family

ID=34393125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/031652 Ceased WO2005030983A2 (en) 2003-09-26 2004-09-27 Amplification of polynucleotides by rolling circle amplification

Country Status (5)

Country Link
US (4) US20050069939A1 (en)
EP (1) EP1664328A2 (en)
CN (1) CN101415838A (en)
CA (1) CA2540087A1 (en)
WO (1) WO2005030983A2 (en)

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Also Published As

Publication number Publication date
US20050069938A1 (en) 2005-03-31
WO2005030983A2 (en) 2005-04-07
US20050074804A1 (en) 2005-04-07
US20050112639A1 (en) 2005-05-26
US20050069939A1 (en) 2005-03-31
CA2540087A1 (en) 2005-04-07
EP1664328A2 (en) 2006-06-07
CN101415838A (en) 2009-04-22

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