WO2005033283A3 - Methods for enhancing the analysis of particle detection - Google Patents

Methods for enhancing the analysis of particle detection Download PDF

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Publication number
WO2005033283A3
WO2005033283A3 PCT/US2004/032244 US2004032244W WO2005033283A3 WO 2005033283 A3 WO2005033283 A3 WO 2005033283A3 US 2004032244 W US2004032244 W US 2004032244W WO 2005033283 A3 WO2005033283 A3 WO 2005033283A3
Authority
WO
WIPO (PCT)
Prior art keywords
enhancing
analysis
methods
particle
particle detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/032244
Other languages
French (fr)
Other versions
WO2005033283A2 (en
Inventor
Robert S Puskas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Singulex Inc
Original Assignee
Singulex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Singulex Inc filed Critical Singulex Inc
Priority to JP2006534113A priority Critical patent/JP2007533971A/en
Priority to US10/574,055 priority patent/US20080021674A1/en
Priority to EP04789395A priority patent/EP1676122A2/en
Publication of WO2005033283A2 publication Critical patent/WO2005033283A2/en
Publication of WO2005033283A3 publication Critical patent/WO2005033283A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0092—Monitoring flocculation or agglomeration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1438—Using two lasers in succession

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Methods for enhancing the analysis of particle detection are provided comprising measuring a first electromagnetic radiation signal provided by a particle, comparing by cross-correlation the electromagnetic radiation signal emitted by the particle, and further applying an analytical filter to the cross-correlation events, thereby enhancing the analysis of the particle emission.
PCT/US2004/032244 2003-09-30 2004-09-30 Methods for enhancing the analysis of particle detection Ceased WO2005033283A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2006534113A JP2007533971A (en) 2003-09-30 2004-09-30 How to improve the accuracy of particle detection
US10/574,055 US20080021674A1 (en) 2003-09-30 2004-09-30 Methods for Enhancing the Analysis of Particle Detection
EP04789395A EP1676122A2 (en) 2003-09-30 2004-09-30 Methods for enhancing the analysis of particle detection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50724303P 2003-09-30 2003-09-30
US60/507,243 2003-09-30

Publications (2)

Publication Number Publication Date
WO2005033283A2 WO2005033283A2 (en) 2005-04-14
WO2005033283A3 true WO2005033283A3 (en) 2005-08-04

Family

ID=34421599

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/032244 Ceased WO2005033283A2 (en) 2003-09-30 2004-09-30 Methods for enhancing the analysis of particle detection

Country Status (3)

Country Link
EP (1) EP1676122A2 (en)
JP (1) JP2007533971A (en)
WO (1) WO2005033283A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8634075B2 (en) 2007-12-19 2014-01-21 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US9182405B2 (en) 2006-04-04 2015-11-10 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US9494598B2 (en) 2006-04-04 2016-11-15 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US11397163B2 (en) 2015-09-07 2022-07-26 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E. V. Method and apparatus for detecting particles, like biological macromolecules or nanoparticles

Families Citing this family (36)

* Cited by examiner, † Cited by third party
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US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US9040305B2 (en) 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US7333197B2 (en) * 2004-11-17 2008-02-19 Honeywell International Inc. Raman detection based flow cytometer
US9452429B2 (en) * 2006-02-02 2016-09-27 E. I. Spectra, Llc Method for mutiplexed microfluidic bead-based immunoassay
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
EP3156799B1 (en) * 2006-04-04 2024-01-24 Novilux, LLC Analyzer and method for highly sensitive detection of analytes
JP6325022B2 (en) * 2006-04-04 2018-05-16 シングレックス，インコーポレイテッド Sensitive system and method for the analysis of troponin
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2041550A4 (en) 2006-06-27 2011-08-24 Biovigilant Systems Inc Pathogen detection by simultaneous size/fluorescence measurement
WO2009029073A1 (en) 2007-08-30 2009-03-05 The Trustees Of Tufts College Methods for determining the concentration of an analyte in solution.
EP2204643B1 (en) * 2007-10-29 2019-08-21 Sysmex Corporation Cell analysis apparatus and cell analysis method
WO2009108223A2 (en) * 2007-11-09 2009-09-03 Biovigilant Systems, Inc. Pathogen detection by simultaneous size/fluorescence measurement
MY174816A (en) 2008-06-10 2020-05-17 Xtralis Technologies Ltd Particle detection
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US8394251B2 (en) * 2008-10-07 2013-03-12 The Board Of Trustees Of The Leland Stanford Junior University Control of chemical reactions using isotachophoresis
KR102032863B1 (en) 2009-05-01 2019-10-16 엑스트랄리스 테크놀로지 리미티드 Improvements to Particle Detectors
JP5678045B2 (en) 2009-06-08 2015-02-25 シンギュレックス・インコーポレイテッド High sensitivity biomarker panel
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
WO2011109379A1 (en) 2010-03-01 2011-09-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
ES2928973T3 (en) 2012-01-11 2022-11-23 Takeda Pharmaceuticals Co Characterization of subvisible particles using a particle analyzer
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
WO2014141994A1 (en) * 2013-03-15 2014-09-18 国立大学法人 東京大学 Particle analyzing method and particle analyzing device
JP7277050B2 (en) 2016-01-29 2023-05-18 ピューリジェン バイオシステムズ， インコーポレイテッド Isotachophoresis for purification of nucleic acids
EP3579973A4 (en) 2017-02-07 2020-07-29 Nodexus Inc. MICROFLUIDIC SYSTEM WITH COMBINED ELECTRICAL AND OPTICAL DETECTION FOR HIGHLY ACCURATE PARTICLE SORTING AND METHOD THEREFOR
US11525767B2 (en) 2017-05-24 2022-12-13 Sony Corporation Optimizing method of suction condition of microparticle and microparticle fractionating device
SG11202000871WA (en) 2017-08-02 2020-02-27 Purigen Biosystems Inc Systems, devices, and methods for isotachophoresis
US20220236164A1 (en) * 2021-01-25 2022-07-28 Becton, Dickinson And Company Method and systems for determing drop delay using scatter signals across spatially separated lasers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5528045A (en) * 1995-04-06 1996-06-18 Becton Dickinson And Company Particle analyzer with spatially split wavelength filter

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DE3208919A1 (en) * 1982-03-12 1983-09-22 Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München ARRANGEMENT FOR MEASURING THE FLUORESCENT POLARISATION
JPH04188045A (en) * 1990-11-21 1992-07-06 Canon Inc Sample measuring device
JPH03128434A (en) * 1989-10-13 1991-05-31 Canon Inc Particle measuring instrument
JPH0792076A (en) * 1993-09-24 1995-04-07 Canon Inc Particle analyzer
JPH0792077A (en) * 1993-09-24 1995-04-07 Canon Inc Particle analyzer
JPH11258144A (en) * 1998-03-13 1999-09-24 Sysmex Corp Particle image-analyzing apparatus
CA2463420A1 (en) * 2001-10-24 2003-05-01 Singulex, Inc. Methods for detecting genetic haplotypes by interaction with probes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US3826364B1 (en) * 1972-05-22 1984-09-25
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5528045A (en) * 1995-04-06 1996-06-18 Becton Dickinson And Company Particle analyzer with spatially split wavelength filter

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9182405B2 (en) 2006-04-04 2015-11-10 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US9494598B2 (en) 2006-04-04 2016-11-15 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US8634075B2 (en) 2007-12-19 2014-01-21 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US8917392B2 (en) 2007-12-19 2014-12-23 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US9239284B2 (en) 2007-12-19 2016-01-19 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US11397163B2 (en) 2015-09-07 2022-07-26 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E. V. Method and apparatus for detecting particles, like biological macromolecules or nanoparticles

Also Published As

Publication number Publication date
WO2005033283A2 (en) 2005-04-14
JP2007533971A (en) 2007-11-22
EP1676122A2 (en) 2006-07-05

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