WO2005059512A3 - Method for efficient transport of small liquid volumes to, from or within microfluidic devices - Google Patents

Method for efficient transport of small liquid volumes to, from or within microfluidic devices Download PDF

Info

Publication number
WO2005059512A3
WO2005059512A3 PCT/US2004/041923 US2004041923W WO2005059512A3 WO 2005059512 A3 WO2005059512 A3 WO 2005059512A3 US 2004041923 W US2004041923 W US 2004041923W WO 2005059512 A3 WO2005059512 A3 WO 2005059512A3
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic devices
small liquid
liquid volumes
efficient transport
zero
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/041923
Other languages
French (fr)
Other versions
WO2005059512A2 (en
WO2005059512A9 (en
Inventor
Roger A Kautz
Barry L Karger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northeastern University Boston
Original Assignee
Northeastern University Boston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northeastern University Boston filed Critical Northeastern University Boston
Priority to US10/582,599 priority Critical patent/US7687269B2/en
Priority to EP04814142A priority patent/EP1711263A2/en
Publication of WO2005059512A2 publication Critical patent/WO2005059512A2/en
Publication of WO2005059512A9 publication Critical patent/WO2005059512A9/en
Publication of WO2005059512A3 publication Critical patent/WO2005059512A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
    • G01R33/30—Sample handling arrangements, e.g. sample cells, spinning mechanisms
    • G01R33/307—Sample handling arrangements, e.g. sample cells, spinning mechanisms specially adapted for moving the sample relative to the MR system, e.g. spinning mechanisms, flow cells or means for positioning the sample inside a spectrometer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/28—Details of apparatus provided for in groups G01R33/44 - G01R33/64
    • G01R33/30—Sample handling arrangements, e.g. sample cells, spinning mechanisms
    • G01R33/302—Miniaturized sample handling arrangements for sampling small quantities, e.g. flow-through microfluidic NMR chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/207497—Molecular oxygen
    • Y10T436/208339—Fuel/air mixture or exhaust gas analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Methods are presented for realizing zero-dispersion segmented flow for transfer of small microfluidic samples onto or within microfluidic analysis or processing devices. Where fluidic systems are in whole or in part made of materials favorable to the zero-dispersion conditions for an indicated solvent/carrier fluid system, the system may be covalently coated to impart the necessary surface properties. This invention is demonstrated in an embodiment where 1 microliter samples (6) are robotically prepared and transferred through 3 meters of capillary tubing (4) to a microcoil NMR probe.
PCT/US2004/041923 2003-12-10 2004-12-10 Method for efficient transport of small liquid volumes to, from or within microfluidic devices Ceased WO2005059512A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/582,599 US7687269B2 (en) 2003-12-10 2004-12-10 Method for efficient transport of small liquid volumes to, from or within microfluidic devices
EP04814142A EP1711263A2 (en) 2003-12-10 2004-12-10 Method for efficient transport of small liquid volumes to, from or within microfluidic devices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52841003P 2003-12-10 2003-12-10
US60/528,410 2003-12-10

Publications (3)

Publication Number Publication Date
WO2005059512A2 WO2005059512A2 (en) 2005-06-30
WO2005059512A9 WO2005059512A9 (en) 2005-08-04
WO2005059512A3 true WO2005059512A3 (en) 2005-09-09

Family

ID=34699866

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/041923 Ceased WO2005059512A2 (en) 2003-12-10 2004-12-10 Method for efficient transport of small liquid volumes to, from or within microfluidic devices

Country Status (3)

Country Link
US (1) US7687269B2 (en)
EP (1) EP1711263A2 (en)
WO (1) WO2005059512A2 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9597644B2 (en) 2003-09-05 2017-03-21 Stokes Bio Limited Methods for culturing and analyzing cells
US8968659B2 (en) * 2003-09-05 2015-03-03 Stokes Bio Limited Sample dispensing
EP1663497B2 (en) 2003-09-05 2020-03-25 Stokes Bio Limited A microfluidic analysis system
US20050191665A1 (en) * 2003-12-29 2005-09-01 Xing Su Composite organic-inorganic nanoclusters
US7655470B2 (en) 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US9477233B2 (en) * 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US20090142853A1 (en) * 2005-08-11 2009-06-04 Eksigent Technologies, Llc Microfluidic system and methods
US7955864B2 (en) 2005-08-22 2011-06-07 Life Technologies Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
US7410763B2 (en) * 2005-09-01 2008-08-12 Intel Corporation Multiplex data collection and analysis in bioanalyte detection
US7704457B2 (en) 2005-11-18 2010-04-27 Patton Charles J Automatic, field portable analyzer using discrete sample aliquots
EP2363205A3 (en) * 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
WO2010068812A1 (en) * 2008-12-10 2010-06-17 Abqmr, Inc. Nuclear magnetic resonance apparatus, methods and associated technology
JP5763652B2 (en) 2009-10-06 2015-08-12 コーニンクレッカ フィリップス エヌ ヴェ Purification of magnetic samples
WO2011066110A1 (en) * 2009-11-25 2011-06-03 Waters Technologies Corporation Coordination of solvent delivery with pre-injection operations in a liquid chromatograph
US8236256B2 (en) * 2010-04-27 2012-08-07 Thomas Friedlander Apparatus and method for efficient and precise transfer of liquids
US9151709B2 (en) * 2010-09-10 2015-10-06 The Curators Of The University Of Missouri Multiple phase flow system for detecting and isolating substances
US20120150025A1 (en) * 2010-09-21 2012-06-14 Siemens Corporation Image Registration Using Interventional Devices
AU2013290020B2 (en) * 2012-07-11 2017-04-20 Nativis, Inc. Miniaturized molecular interrogation and data system
US9945820B2 (en) 2012-11-30 2018-04-17 Agilent Technologies, Inc. Mixer bypass sample injection for liquid chromatography
US9753026B1 (en) * 2012-12-31 2017-09-05 Techshot, Inc. Cell processing cartridge for miniature cytometer
GB201303913D0 (en) * 2013-03-05 2013-04-17 Touchlight Genetics Ltd Synthesis apparatus and method
JP6654132B2 (en) 2013-03-15 2020-02-26 ネイティヴィス、インコーポレイテッド Controller and flexible coil for administering treatment such as cancer treatment
CN104634805B (en) * 2013-11-13 2017-05-03 中国科学院苏州纳米技术与纳米仿生研究所 Method for measuring underwater contact angle of water strider leg simulated structure
WO2015116978A1 (en) 2014-01-31 2015-08-06 Carnegie Mellon University Device and method for clinical data sampling and specimen banking
KR102308590B1 (en) 2014-02-27 2021-10-01 엘리멘탈 사이언티픽, 인코포레이티드 System for collecting liquid samples from a distance
US10585075B2 (en) 2014-02-27 2020-03-10 Elemental Scientific, Inc. System for collecting liquid samples
DE112016002907T5 (en) * 2015-06-26 2018-03-08 Elemental Scientific, Inc. System for taking fluid samples
CN105929348B (en) * 2016-05-05 2018-09-07 中国科学技术大学 A kind of nuclear magnetic resonance spectrometer and the nuclear magnetic resonance spectrometer control device based on FPGA
US10258741B2 (en) 2016-12-28 2019-04-16 Cequr Sa Microfluidic flow restrictor and system
WO2019006190A1 (en) * 2017-06-28 2019-01-03 Bio-Rad Laboratories, Inc. System and method for droplet detection
CN107727838B (en) * 2017-09-30 2019-10-25 西南大学 A micro-volume waste liquid collection device suitable for microfluidic chips
WO2019089979A1 (en) 2017-11-01 2019-05-09 Bio-Rad Laboratories, Inc. Microfluidic system and method for arranging objects
EP3505232B1 (en) * 2017-12-28 2025-04-30 Satisloh AG Coating system with a device for mixing and/or dosing liquid coating materials and method for coloring optical glasses
FR3082440B1 (en) * 2018-06-14 2020-12-11 Paris Sciences Lettres Quartier Latin MATERIAL TRANSFER METHOD IN A MICROFLUIDIC OR MILLIFLUIDIC DEVICE
WO2021011884A1 (en) * 2019-07-18 2021-01-21 Board Of Regents, The University Of Texas System Nano flow sensors
GB2595519A (en) * 2020-05-29 2021-12-01 Univ Leiden Assemblies and methods

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130098A (en) * 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US20020187074A1 (en) * 2001-06-07 2002-12-12 Nanostream, Inc. Microfluidic analytical devices and methods
US20030006140A1 (en) * 2001-02-28 2003-01-09 Giacomo Vacca Microfluidic control using dielectric pumping
US6508988B1 (en) * 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US20030124736A1 (en) * 1998-08-05 2003-07-03 Caliper Technologies Corp. Open-field serial to parallel converter
US20030232450A1 (en) * 2002-06-13 2003-12-18 Yoshikazu Yoshida Microfluidic device and method for producing the same

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3479141A (en) * 1967-05-17 1969-11-18 Technicon Corp Method and apparatus for analysis
WO1996023220A1 (en) * 1995-01-27 1996-08-01 Northeastern University Polyvinyl alcohol (pva) based covalently bonded stable hydrophilic coating for capillary electrophoresis
US6706519B1 (en) * 1999-06-22 2004-03-16 Tecan Trading Ag Devices and methods for the performance of miniaturized in vitro amplification assays
US6524456B1 (en) * 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
GB2359765B (en) * 2000-03-02 2003-03-05 Univ Newcastle Capillary reactor distribution device and method
US6645432B1 (en) * 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US7129091B2 (en) * 2002-05-09 2006-10-31 University Of Chicago Device and method for pressure-driven plug transport and reaction
US6808745B2 (en) * 2002-08-22 2004-10-26 Eastman Kodak Company Method of coating micro-electromechanical devices
US6865939B2 (en) * 2002-09-16 2005-03-15 Sandia Naitonal Laboratories Fluorinated silica microchannel surfaces
GB2395196B (en) * 2002-11-14 2006-12-27 Univ Cardiff Microfluidic device and methods for construction and application

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130098A (en) * 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US20030124736A1 (en) * 1998-08-05 2003-07-03 Caliper Technologies Corp. Open-field serial to parallel converter
US6508988B1 (en) * 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US20030006140A1 (en) * 2001-02-28 2003-01-09 Giacomo Vacca Microfluidic control using dielectric pumping
US20020187074A1 (en) * 2001-06-07 2002-12-12 Nanostream, Inc. Microfluidic analytical devices and methods
US20030232450A1 (en) * 2002-06-13 2003-12-18 Yoshikazu Yoshida Microfluidic device and method for producing the same

Also Published As

Publication number Publication date
US7687269B2 (en) 2010-03-30
WO2005059512A2 (en) 2005-06-30
US20070117212A1 (en) 2007-05-24
EP1711263A2 (en) 2006-10-18
WO2005059512A9 (en) 2005-08-04

Similar Documents

Publication Publication Date Title
US7252928B1 (en) Methods for prevention of surface adsorption of biological materials to capillary walls in microchannels
US7314567B2 (en) Method for transferring heterogeneous liquids in microchannels without the occurrence of mixing
EP1322936A2 (en) Microfluidic devices and methods of use
WO2000030751A3 (en) Microplate sample and reagent loading system
US10926258B2 (en) Microfluidic probe head for processing a sequence of liquid volumes separated by spacers
US12540948B2 (en) Systems and methods for generating droplets and performing digital analyses
EP0964428A3 (en) Miniaturized device for sample processing and mass spectroscopic detection of liquid phase samples
ATE463732T1 (en) SYSTEM FOR TRANSPORTING MICROSCOPIC QUANTITIES OF LIQUID
WO2004042367A3 (en) Microfluidic system utilizing thin-film layers to route fluid
EP0961110A3 (en) Device for preparing thin liquid samples for microscopic analysis
WO2004111601A3 (en) Couette device and method to study solids deposition from flowing fluids
CA2555060C (en) Method and device for manipulating liquids in microfluidic systems
WO2007111651A3 (en) Systems and methods of sample processing and fluid control in a fluidic system
JPS59501994A (en) chemical drug drop reactor
US20140099241A1 (en) Novel Micropores and Methods of Making and Using Thereof
EP1489303A3 (en) Reducing working fluid dilution in liquid systems
US20190201903A1 (en) Improvements in or relating to a reagent cartridge
WO2009039239A3 (en) Lateral flow assay using centrifugal force
Chang et al. A microchannel immunoassay chip with ferrofluid actuation to enhance the biochemical reaction
AU2012304228B2 (en) Centrifugally-enhanced capture method and device
Grasso et al. Microfluidic networks for surface plasmon resonance imaging real-time kinetics experiments
AU2002229977A1 (en) Biochemical method and apparatus for detecting genetic characteristics
US10357770B2 (en) Microfluidic probe for modulating insertion of liquid spacers
WO2002065123A3 (en) Biochemical method and apparatus for detecting protein characteristics
WO2002079749A3 (en) System and method for generating a profile of particulate components of a body fluid sample

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

COP Corrected version of pamphlet

Free format text: PAGES 1/9-9/9, DRAWINGS, REPLACED BY NEW PAGES 1/9-9/9

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2007117212

Country of ref document: US

Ref document number: 10582599

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2004814142

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004814142

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2004814142

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 10582599

Country of ref document: US